专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US06357163B1 Use of nucleic acid analogues in diagnostics and analytical procedures 失效
标题翻译：在诊断和分析程序中使用核酸类似物
公开(公告)号：US06357163B1
公开(公告)日：2002-03-19
申请号：US08150156
申请日：1994-05-04
申请人： Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg
发明人： Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg
IPC分类号： C12Q168
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： Methods of capture, recognition, detection, identification or quantitation of nucleic acids and diagnostics uses generally are described in which are used: (a) a peptide nucleic acid (PNA) comprising a polyamide backbone bearing a plurality of ligands at respective spaced locations along said backbone, said ligands being each independently naturally occurring nucleobases, non-naturally occurring nucleobases or nucleobase-binding groups, each said ligand being bound directly or indirectly to a nitrogen atom in said backbone, and said ligand bearing nitrogen atoms mainly being separated from one another in said backbone by from 4 to 8 intervening atoms; or (b) a nucleic acid analogue capable of hybridizing to a nucleic acid of complementary sequence to form a hybrid which is more stable against denaturation by heat than a hybrid between the conventional deoxyribonucleotide corresponding to said analogue and said nucleic acid; or (c) a nucleic acid analogue capable of hybridizing to a double stranded nucleic acid in which one strand has a sequence complementary to said analogue, so as to displace the other strand from said one strand.
摘要翻译：通常描述了核酸和诊断用途的捕获，识别，检测，鉴定或定量的方法，其中使用：（a）肽核酸（PNA），其包含聚酰胺主链，所述聚酰胺主链在所述所述配体各自独立地是天然存在的核碱基，非天然存在的核碱基或核碱基结合基团，每个所述配体直接或间接地结合到所述主链中的氮原子，并且所述配位体氮原子主要彼此分离在所述骨架中通过4至8个插入的原子; 或（b）能够与互补序列核酸杂交的核酸类似物，以形成与对应于所述类似物的所述类似物和所述核酸的常规脱氧核糖核苷酸之间的杂交体相比，通过加热变性更稳定的杂交体; 或（c）能够与双链核酸杂交的核酸类似物，其中一条链具有与所述类似物互补的序列，以便从所述一条链取代另一条链。

2. 发明授权

US5641625A Cleaving double-stranded DNA with peptide nucleic acids 失效
标题翻译：用肽核酸切割双链DNA
公开(公告)号：US5641625A
公开(公告)日：1997-06-24
申请号：US88658
申请日：1993-07-02
申请人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
发明人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
IPC分类号： C12N15/09 , A61K31/00 , A61K31/52 , A61K31/522 , A61K31/70 , A61K31/7088 , A61K31/7125 , A61K38/00 , A61K48/00 , A61P31/00 , A61P31/12 , A61P35/00 , A61P35/02 , A61P43/00 , C07C233/46 , C07C237/22 , C07D239/24 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07H21/02 , C07H21/04 , C07K7/06 , C07K7/08 , C07K14/00 , C08G69/00 , C08G69/08 , C12N15/113 , C12Q1/68
CPC分类号： C12Q1/6813 , B82Y5/00 , C07H21/00 , C07K14/003 , C12N15/113 , A61K38/00 , C12N2310/15 , C12N2310/3181
摘要： Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.
摘要翻译：肽核酸和肽核酸的类似物用于与核酸形成双链体，三链体和其他结构并修饰核酸。肽核酸及其类似物也用于通过例如核酸的转录停止，转录起始和位点特异性切割来调节蛋白质活性。

3. 发明授权

US06770738B1 Higher order structure and binding of peptide nucleic acids 失效
标题翻译：高级结构和肽核酸的结合
公开(公告)号：US06770738B1
公开(公告)日：2004-08-03
申请号：US09442054
申请日：1999-11-16
申请人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
发明人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
IPC分类号： A61K3800
CPC分类号： C12N15/113 , A61K38/00 , B82Y5/00 , C07H21/00 , C07K14/003 , C12N2310/15 , C12N2310/3181 , C12Q1/6813 , C12Q2525/107 , C12Q2537/119
摘要： Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.
摘要翻译：肽核酸和肽核酸的类似物用于与核酸形成双链体，三链体和其他结构并修饰核酸。肽核酸及其类似物也用于通过例如核酸的转录停止，转录起始和位点特异性切割来调节蛋白质活性。

4. 发明授权

US5539082A Peptide nucleic acids 失效
公开(公告)号：US5539082A
公开(公告)日：1996-07-23
申请号：US54363
申请日：1993-04-26
申请人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
发明人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
IPC分类号： C12N15/09 , A61K31/00 , A61K31/52 , A61K31/522 , A61K31/70 , A61K31/7088 , A61K38/00 , A61K48/00 , A61P31/00 , A61P31/12 , A61P35/00 , C07C233/46 , C07C237/22 , C07D239/24 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07K5/06 , C07K7/06 , C07K7/08 , C07K14/00 , C08G69/08 , C12N15/113 , C12Q1/68 , C07K7/00 , C07D239/00 , C07H5/04 , C07H21/04
CPC分类号： C12N15/113 , B82Y5/00 , C07H21/00 , C07K14/003 , C07K5/06026 , C07K7/06 , C07K7/08 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , A61K38/00 , C12N2310/15 , C12N2310/3181
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.

5. 发明申请

US20120115136A1 PNA DIAGNOSTIC USE 审中-公开
标题翻译： PNA诊断用途
公开(公告)号：US20120115136A1
公开(公告)日：2012-05-10
申请号：US13190164
申请日：2011-07-25
申请人： Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg
发明人： Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg
IPC分类号： C12Q1/68 , C07K17/02 , C12N9/96 , C07K2/00
CPC分类号： C07H21/00 , A61K38/00 , C07K5/06026 , C07K5/06139 , C07K7/06 , C07K7/08 , C07K14/003 , C12Q1/68 , C12Q1/6813 , C12Q1/6832 , C12Q1/6869 , C12Q2535/107 , C12Q2525/107
摘要： The present invention pertains to certain nucleic acid analogs and related kits that are useful for the capture, recognition, detection, identification, or quantification of certain chemical or biological entities.
摘要翻译：本发明涉及可用于某些化学或生物实体的捕获，识别，检测，鉴定或定量的某些核酸类似物和相关试剂盒。

6. 发明授权

US06710164B1 Peptide nucleic acids having enhanced binding affinity, sequence specificity and solubility 失效
标题翻译：肽核酸
公开(公告)号：US06710164B1
公开(公告)日：2004-03-23
申请号：US09230088
申请日：1999-03-10
申请人： Peter E. Nielsen , Michael Egholm , Rolf H. Berg , Ole Buchardt
发明人： Peter E. Nielsen , Michael Egholm , Rolf H. Berg , Ole Buchardt
IPC分类号： A61K3800
CPC分类号： C07K5/06139 , C07K14/003 , C12Q1/68
摘要： A novel class of compounds known as peptide nucleic acids, bind complementary DNA and RNA strands, and generally do so more strongly than the corresponding DNA or RNA strands while exhibiting increased sequence specificity and solubility. The peptide nucleic acids comprise ligands selected from a group consisting of naturally-occurring nucleobases and non-naturally-occurring nucleobases, including 2,6-diaminopurine, attached to a polyamide backbone, and contain alkyl amine side chains.
摘要翻译：被称为肽核酸的新一类化合物结合互补DNA和RNA链，并且通常比相应的DNA或RNA链更强烈地显示出增加的序列特异性和溶解性。肽核酸包含选自天然存在的核碱基和非天然存在的核碱基的配体，包括连接到聚酰胺主链上的2,6-二氨基嘌呤，并且含有烷基胺侧链。

7. 发明授权

US5986053A Peptide nucleic acids complexes of two peptide nucleic acid strands and one nucleic acid strand 失效
标题翻译：两个肽核酸链和一个核酸链的肽核酸复合物
公开(公告)号：US5986053A
公开(公告)日：1999-11-16
申请号：US471907
申请日：1995-06-07
申请人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
发明人： David J. Ecker , Ole Buchardt , Michael Egholm , Peter E. Nielsen , Rolf H. Berg , Niels E. Mollegaard
IPC分类号： C12N15/09 , A61K31/00 , A61K31/52 , A61K31/522 , A61K31/70 , A61K31/7088 , A61K31/7125 , A61K38/00 , A61K48/00 , A61P31/00 , A61P31/12 , A61P35/00 , A61P35/02 , A61P43/00 , C07C233/46 , C07C237/22 , C07D239/24 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07H21/02 , C07H21/04 , C07K7/06 , C07K7/08 , C07K14/00 , C08G69/00 , C08G69/08 , C12N15/113 , C12Q1/68 , C07K2/00
CPC分类号： C12Q1/6813 , B82Y5/00 , C07H21/00 , C07K14/003 , C12N15/113 , A61K38/00 , C12N2310/15 , C12N2310/3181
摘要： Peptide nucleic acids and analogues of peptide nucleic acids are used to form duplex, triplex, and other structures with nucleic acids and to modify nucleic acids. The peptide nucleic acids and analogues thereof also are used to modulate protein activity through, for example, transcription arrest, transcription initiation, and site specific cleavage of nucleic acids.
摘要翻译：肽核酸和肽核酸的类似物用于与核酸形成双链体，三链体和其他结构并修饰核酸。肽核酸及其类似物也用于通过例如核酸的转录停止，转录起始和位点特异性切割来调节蛋白质活性。

8. 发明授权

US5773571A Peptide nucleic acids 失效
公开(公告)号：US5773571A
公开(公告)日：1998-06-30
申请号：US595387
申请日：1996-02-01
申请人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
发明人： Peter E. Nielsen , Ole Buchardt , Michael Egholm , Rolf H. Berg
IPC分类号： C12N15/09 , A61K31/00 , A61K31/52 , A61K31/522 , A61K31/70 , A61K31/7088 , A61K38/00 , A61K48/00 , A61P31/00 , A61P31/12 , A61P35/00 , C07C233/46 , C07C237/22 , C07D239/24 , C07D239/54 , C07D473/18 , C07D473/34 , C07H21/00 , C07K5/06 , C07K7/06 , C07K7/08 , C07K14/00 , C08G69/08 , C12N15/113 , C12Q1/68 , C07K5/00
CPC分类号： C12N15/113 , B82Y5/00 , C07H21/00 , C07K14/003 , C07K5/06026 , C07K7/06 , C07K7/08 , C12Q1/68 , C12Q1/6813 , C12Q1/6869 , A61K38/00 , C12N2310/15 , C12N2310/3181
摘要： A novel class of compounds, known as peptide nucleic acids, bind complementary ssDNA and RNA strands more strongly than a corresponding DNA. The peptide nucleic acids generally comprise ligands such as naturally occurring DNA bases attached to a peptide backbone through a suitable linker.

9. 发明授权

US6107470A Histidine-containing peptide nucleic acids 失效
标题翻译：含组氨酸的肽核酸
公开(公告)号：US6107470A
公开(公告)日：2000-08-22
申请号：US225146
申请日：1999-01-04
申请人： Peter E. Nielsen , Ole Buchardt, deceased , Rolf H. Berg , Michael Egholm
发明人： Peter E. Nielsen , Ole Buchardt, deceased , Rolf H. Berg , Michael Egholm
IPC分类号： C07K14/00 , C07H21/02 , C07H19/00 , C07H21/04 , C12Q1/68
CPC分类号： C07K14/003
摘要： Peptide nucleic acids containing histidine moieties are provided. These compounds have applications including diagnostics, research and potential therapeutics.
摘要翻译：提供含有组氨酸部分的肽核酸。这些化合物具有诊断，研究和潜在治疗的应用。

10. 发明授权

US5373053A Peptide synthesis method and solid support for use in the method 失效
标题翻译：肽合成方法和固体支持用于该方法
公开(公告)号：US5373053A
公开(公告)日：1994-12-13
申请号：US990584
申请日：1992-12-14
申请人： Rolf H. Berg , Kristoffer Almdal , Walther B. Pedersen , Arne Holm , James P. Tam , Robert B. Merrifield
发明人： Rolf H. Berg , Kristoffer Almdal , Walther B. Pedersen , Arne Holm , James P. Tam , Robert B. Merrifield
IPC分类号： C07K1/04 , G01N33/545 , C07C103/52 , C07D51/52
CPC分类号： G01N33/545 , C07K1/042
摘要： A method for the solid-phase synthesis of peptides or proteins in high yield and high purity uses a solid support consisting of a functionalized polystyrene-grafted polymer substrate, the grafted polystyrene chains being substantially non-cross-linked and having a chain molecular weight, not including optional non-reactive substituents, of at least 200,000, preferably in the range of 600,000-1,200,000. Particularly suitable polymer substrates are substrates of a polyolefin such as polyethylene. The method is particularly well-suited to the compartmentalized synthesis of a multitude of peptides or proteins in a parallel and substantially simultaneous fashion.Preferred embodiments of a solid support for performing the synthesis are prepared from thin polyethylene sheet or film which has been grafted with polystyrene chains in a radical-initiated process in which the polyethylene sheet or film is immersed in a solution of optionally substituted styrene monomer in an alcohol such as methanol, the volume percentage of styrene in the solution preferably being about 30% v/v, and subjected to gamma irradiation.
摘要翻译：以高产率和高纯度固相合成肽或蛋白质的方法使用由官能化聚苯乙烯接枝的聚合物基质组成的固体支持物，所述接枝聚苯乙烯链基本上是非交联并具有链分子量，不包括任选的非反应性取代基，为至少20万，优选在600,000-1200,000的范围内。特别合适的聚合物基材是聚烯烃如聚乙烯的基材。该方法特别适合于以平行和基本上同时的方式区分多种肽或蛋白质的合成。用于进行合成的固体支持物的优选实施方案由在自由基引发的方法中已经用聚苯乙烯链接枝的薄聚乙烯片或薄膜制备，其中聚乙烯片或薄膜浸渍在任选取代的苯乙烯单体的溶液中醇如甲醇，溶液中苯乙烯的体积百分比优选为约30％v / v，并进行γ照射。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式