专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20090011956A1 Versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways 有权
标题翻译：用于编程生物分子自组装途径的多功能核酸发夹基序
公开(公告)号：US20090011956A1
公开(公告)日：2009-01-08
申请号：US12152893
申请日：2008-05-16
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： C40B50/04 , C40B50/02 , C07H21/00
CPC分类号： C12N15/63 , B82Y5/00 , C12N15/10 , C12N15/1031 , C12Q1/6844 , C12Q2525/301 , C12Q2525/161
摘要： The present invention relates generally to programming of biomolecular self-assembly pathways and related methods and constructs. A versatile nucleic acid hairpin motif for programming biomolecular self-assembly pathways for a wide variety of dynamic functions, reaction graphs for specifying pathways, and methods of using the hairpin motif are provided.
摘要翻译：本发明一般涉及生物分子自组装途径的编程以及相关方法和结构。提供了用于编程用于各种动态功能的生物分子自组装途径的多功能核酸发夹基序，用于指定途径的反应图和使用发夹基序的方法。

2. 发明申请

US20080214488A1 TRIGGERED RNAi 有权
公开(公告)号：US20080214488A1
公开(公告)日：2008-09-04
申请号：US12040735
申请日：2008-02-29
申请人： Niles A. Pierce , Peng Yin
发明人： Niles A. Pierce , Peng Yin
IPC分类号： A61K31/7105 , C12N15/87 , A61P35/00
CPC分类号： A61K31/7105 , C12N15/111 , C12N2310/14 , C12N2310/16 , C12N2310/53 , C12N2320/50 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, a method of silencing a target gene is provided. The method comprises providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene; providing a first substrate monomer to the cell, wherein the first substrate monomer comprises a silencing target complement region that is substantially complementary to a portion of the silencing target gene, and an initiator complement region that is substantially complementary to a portion of the initiator; and providing a second substrate monomer to the cell, wherein the second substrate monomer comprises a silencing target region that is substantially complementary to the silencing target complement region; wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA) which silences the silencing target gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默靶基因的方法。该方法包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同; 向所述细胞提供第一底物单体，其中所述第一底物单体包含与所述沉默靶基因的一部分基本上互补的沉默靶补体区和与所述引发剂的一部分基本上互补的引发剂补体区; 以及向所述细胞提供第二底物单体，其中所述第二底物单体包含与沉默靶补体区基本上互补的沉默靶区; 其中检测靶与引发剂的结合引发了使沉默靶基因沉默的失活剂双链RNA（灭活酶dsRNA）的形成。

3. 发明授权

US08318921B2 Triggered RNAi 有权
标题翻译：触发RNAi
公开(公告)号：US08318921B2
公开(公告)日：2012-11-27
申请号：US12040735
申请日：2008-02-29
申请人： Niles A. Pierce , Peng Yin
发明人： Niles A. Pierce , Peng Yin
IPC分类号： C07H21/04 , C07H21/02 , C12N15/85 , A61K48/00
CPC分类号： A61K31/7105 , C12N15/111 , C12N2310/14 , C12N2310/16 , C12N2310/53 , C12N2320/50 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, a method of silencing a target gene is provided. The method comprises providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene; providing a first substrate monomer to the cell, wherein the first substrate monomer comprises a silencing target complement region that is substantially complementary to a portion of the silencing target gene, and an initiator complement region that is substantially complementary to a portion of the initiator; and providing a second substrate monomer to the cell, wherein the second substrate monomer comprises a silencing target region that is substantially complementary to the silencing target complement region; wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA) which silences the silencing target gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默靶基因的方法。该方法包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同; 向所述细胞提供第一底物单体，其中所述第一底物单体包含与所述沉默靶基因的一部分基本上互补的沉默靶补体区和与所述引发剂的一部分基本上互补的引发剂补体区; 以及向所述细胞提供第二底物单体，其中所述第二底物单体包含与沉默靶补体区基本上互补的沉默靶区; 其中检测靶与引发剂的结合引发了使沉默靶基因沉默的失活剂双链RNA（灭活酶dsRNA）的形成。

4. 发明授权

US09834439B2 Biomolecular self-assembly 有权
公开(公告)号：US09834439B2
公开(公告)日：2017-12-05
申请号：US13186228
申请日：2011-07-19
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： C07H21/02 , B82Y15/00
CPC分类号： B82Y15/00
摘要： The present invention relates generally to programming of biomolecular self-assembly pathways and related methods and constructs for self-assembly of prescribed two and three-dimensional structures.

5. 发明授权

US08962241B2 Triggered molecular geometry based bioimaging probes 有权
标题翻译：触发的基于分子几何的生物成像探针
公开(公告)号：US08962241B2
公开(公告)日：2015-02-24
申请号：US13186315
申请日：2011-07-19
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04 , C12P19/14 , C07K1/13 , G01N21/64
CPC分类号： C07K1/13 , G01N2021/6441
摘要： The present embodiments relate to engineering imaging probes based on “triggered molecular geometry.” Upon detection of a molecular signal, nucleic acid hairpin monomers assemble an imageable molecular shape with prescribed geometry. In some embodiments the prescribed shape can be imaged directly. In some embodiments, the prescribed shape can serve as a spatial organizer or amplification scheme for other imaging entities, such as fluorophore and fluorescent proteins.
摘要翻译：本实施例涉及基于“触发分子几何”的工程成像探针。在检测到分子信号时，核酸发夹单体组装具有规定几何形状的可成像分子形状。在一些实施例中，可以直接成像规定的形状。在一些实施例中，规定的形状可以用作其它成像实体（例如荧光团和荧光蛋白）的空间组织器或放大方案。

6. 发明授权

US08497364B2 Triggered RNAi 有权
标题翻译：触发RNAi
公开(公告)号：US08497364B2
公开(公告)日：2013-07-30
申请号：US12395489
申请日：2009-02-27
申请人： Niles A. Pierce , Peng Yin
发明人： Niles A. Pierce , Peng Yin
IPC分类号： C07H21/04 , C07H21/02 , A61K48/00
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/14 , C12N2310/531 , C12N2320/11 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, methods of silencing or modulating the expression of a target gene are provided. The methods generally comprise providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene, wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA). The inactivator dsRNA can silence or modulate the expression of the silencing target gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默或调节靶基因表达的方法。所述方法通常包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同，其中检测靶与引发剂的结合引发灭活双链RNA的形成（灭活酶dsRNA）。灭活剂dsRNA可以沉默或调节沉默靶基因的表达。

7. 发明授权

US08241854B2 Triggered RNAi 有权
标题翻译：触发RNAi
公开(公告)号：US08241854B2
公开(公告)日：2012-08-14
申请号：US12454743
申请日：2009-05-22
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： C12Q1/68 , C07H21/02 , C07H21/04 , C12N15/11 , C12N15/00
CPC分类号： C12Q1/6897 , C12N15/113 , C12N2310/14 , C12N2310/16 , C12N2310/3519 , C12N2310/531 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, methods of silencing or modulating the expression of a marker gene are provided. The methods generally comprise providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene, wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA). The inactivator dsRNA can silence the silencing target gene to modulate the expression of a marker gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默或调节标记基因表达的方法。所述方法通常包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同，其中检测靶与引发剂的结合引发灭活双链RNA的形成（灭活酶dsRNA）。灭活dsRNA可以使沉默靶基因沉默，调节标记基因的表达。

8. 发明申请

US20120021410A1 TRIGGERED MOLECULAR GEOMETRY BASED BIOIMAGING PROBES 有权
标题翻译：基于激发分子几何的生物探针探针
公开(公告)号：US20120021410A1
公开(公告)日：2012-01-26
申请号：US13186315
申请日：2011-07-19
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： G01N21/64 , C07K2/00 , C12Q1/68 , C07H21/00 , C07H1/00
CPC分类号： C07K1/13 , G01N2021/6441
摘要： The present embodiments relate to engineering imaging probes based on “triggered molecular geometry.” Upon detection of a molecular signal, nucleic acid hairpin monomers assemble an imageable molecular shape with prescribed geometry. In some embodiments the prescribed shape can be imaged directly. In some embodiments, the prescribed shape can serve as a spatial organizer or amplification scheme for other imaging entities, such as fluorophore and fluorescent proteins.
摘要翻译：本实施例涉及基于“触发分子几何”的工程成像探针。在检测到分子信号时，核酸发夹单体组装具有规定几何形状的可成像分子形状。在一些实施例中，可以直接成像规定的形状。在一些实施例中，规定的形状可以用作其它成像实体（例如荧光团和荧光蛋白）的空间组织器或放大方案。

9. 发明申请

US20100035233A1 Triggered RNAi 有权
标题翻译：触发RNAi
公开(公告)号：US20100035233A1
公开(公告)日：2010-02-11
申请号：US12454743
申请日：2009-05-22
申请人： Peng Yin , Niles A. Pierce
发明人： Peng Yin , Niles A. Pierce
IPC分类号： C12Q1/70 , C12Q1/68
CPC分类号： C12Q1/6897 , C12N15/113 , C12N2310/14 , C12N2310/16 , C12N2310/3519 , C12N2310/531 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, methods of silencing or modulating the expression of a marker gene are provided. The methods generally comprise providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene, wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA). The inactivator dsRNA can silence the silencing target gene to modulate the expression of a marker gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默或调节标记基因表达的方法。所述方法通常包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同，其中检测靶与引发剂的结合引发灭活双链RNA的形成（灭活酶dsRNA）。灭活dsRNA可以使沉默靶基因沉默，调节标记基因的表达。

10. 发明申请

US20090247615A1 TRIGGERED RNAi 有权
公开(公告)号：US20090247615A1
公开(公告)日：2009-10-01
申请号：US12395489
申请日：2009-02-27
申请人： Niles A. Pierce , Peng Yin
发明人： Niles A. Pierce , Peng Yin
IPC分类号： A61K31/7105 , C07H21/02 , C12N5/06
CPC分类号： C12N15/113 , C12N15/111 , C12N2310/14 , C12N2310/531 , C12N2320/11 , Y02P20/582
摘要： The present application relates to methods and compositions for triggering RNAi. Triggered RNAi is highly versatile because the silencing targets are independent of the detection targets. In some embodiments, methods of silencing or modulating the expression of a target gene are provided. The methods generally comprise providing an initiator to a cell comprising a detection target and a silencing target gene, wherein the detection target is different from the silencing target gene, wherein binding of the detection target to the initiator initiates formation of an inactivator double-stranded RNA (inactivator dsRNA). The inactivator dsRNA can silence or modulate the expression of the silencing target gene.
摘要翻译：本申请涉及用于触发RNAi的方法和组合物。触发的RNAi是非常通用的，因为沉默靶标与检测目标无关。在一些实施方案中，提供沉默或调节靶基因表达的方法。所述方法通常包括向包含检测靶和沉默靶基因的细胞提供引发剂，其中检测靶与沉默靶基因不同，其中检测靶与引发剂的结合引发灭活双链RNA的形成（灭活酶dsRNA）。灭活剂dsRNA可以沉默或调节沉默靶基因的表达。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式