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1. 发明授权

US07696408B2 Method to enhance Agrobacterium-mediated transformation of plants 失效
标题翻译：增强农杆菌介导的植物转化的方法
公开(公告)号：US07696408B2
公开(公告)日：2010-04-13
申请号：US10813482
申请日：2004-03-30
申请人： Paula Olhoft , David A. Somers
发明人： Paula Olhoft , David A. Somers
IPC分类号： C12N15/82 , A01H4/00
CPC分类号： C12N15/8205 , C12N5/0025
摘要： The invention provides a method to enhance Agrobacterium-mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.
摘要翻译：本发明提供了一种增强农杆菌介导的植物细胞，部分和组织转化从而增强转基因植物生产的方法。

2. 发明授权

US06759573B2 Method to enhance agrobacterium-mediated transformation of plants 有权
标题翻译：增强农杆菌介导的植物转化的方法
公开(公告)号：US06759573B2
公开(公告)日：2004-07-06
申请号：US09738398
申请日：2000-12-15
申请人： Paula Olhoft , David A. Somers
发明人： Paula Olhoft , David A. Somers
IPC分类号： A04L500
CPC分类号： C12N15/8205 , C12N5/0025
摘要： The invention provides a method to enhance Agrobacterium-mediated transformation of plant cells, parts and tissues, thereby enhancing the production of transgenic plants.
摘要翻译：本发明提供了一种增强农杆菌介导的植物细胞，部分和组织转化从而增强转基因植物生产的方法。

3. 发明授权

US5994123A Sugarcane bacilliform virus promoter 失效
公开(公告)号：US5994123A
公开(公告)日：1999-11-30
申请号：US694869
申请日：1996-08-09
申请人： Neil Olszewski , Iris Tzafrir , David A. Somers , Benham Lockhart , Kimberly Torbert
发明人： Neil Olszewski , Iris Tzafrir , David A. Somers , Benham Lockhart , Kimberly Torbert
IPC分类号： A01H5/00 , C07K14/01 , C12N15/82 , A01H4/00 , C07H21/04 , C12N5/14 , C12P19/34
CPC分类号： C07K14/005 , C12N15/8216 , C12N15/8222 , C12N2730/00022
摘要： An isolated and purified DNA molecule comprising a sugarcane bacilliform virus promoter and expression cassettes comprising said promoter are provided. Also provided is a method of using a sugarcane bacilliform virus promoter to express proteins, RNA transcripts, or mixtures thereof, in transgenic plants.

4. 发明授权

US06414222B1 Gene combinations for herbicide tolerance in corn 失效
标题翻译：玉米除草剂耐受性的基因组合
公开(公告)号：US06414222B1
公开(公告)日：2002-07-02
申请号：US08697826
申请日：1996-08-30
申请人： Burle G. Gengenbach , David A. Somers , Margaret A. Egli , Lorelei C. Marshall , Donald L. Wyse , Shelia M. Lutz , Kevin L. Van Dee , William B. Parker
发明人： Burle G. Gengenbach , David A. Somers , Margaret A. Egli , Lorelei C. Marshall , Donald L. Wyse , Shelia M. Lutz , Kevin L. Van Dee , William B. Parker
IPC分类号： A01H500
CPC分类号： C12N9/93 , C12N15/8247 , C12N15/8274
摘要： The present invention provides methods for preparing herbicide tolerant corn plants. Also provided are herbicide tolerant corn plants, as well as seeds and progeny derived from these plants.
摘要翻译：本发明提供了制备耐除草剂玉米植物的方法。还提供了除草剂耐受性玉米植物，以及源自这些植物的种子和后代。

5. 发明授权

US6143952A Modified pseudomonas oleovorans phaC1 nucleic acids encoding bispecific polyhydroxyalkanoate polymerase 失效
标题翻译：编码双特异性聚羟基链烷酸酯聚合酶的改良油菜素phaC1核酸
公开(公告)号：US6143952A
公开(公告)日：2000-11-07
申请号：US52689
申请日：1998-03-31
申请人： Friedrich Srienc , John K. Jackson , David A. Somers
发明人： Friedrich Srienc , John K. Jackson , David A. Somers
IPC分类号： C12N9/10 , C12N15/31 , C12P7/62 , A01H5/00 , C12N1/21 , C12N15/52 , C12N15/70 , C12N15/81 , C12N15/82
CPC分类号： C12N9/1025 , C12P7/625
摘要： A genetically engineered Pseudomonas oleovorans phaC1 polyhydroxyalkanoate (PHA) polymerase having tailored substrate specificity is provided. The modified PHA polymerase is preferably a "bispecific" PHA polymerase capable of copolymerizing a short chain length monomer and a medium chain length monomer is provided. Methods for making the modified PHA polymerase and for making nucleic acids encoding the modified PHA polymerase are also disclosed, as are methods of producing PHA using the modified PHA polymerase. The invention further includes methods to assay for altered substrate specificity.
摘要翻译：提供了具有定制底物特异性的遗传工程化油菜素聚合酶多聚羟基链烷酸酯（PHA）聚合酶。修饰的PHA聚合酶优选提供能够共聚短链长度单体和中链长度单体的“双特异性”PHA聚合酶。还公开了制备修饰的PHA聚合酶和制备编码修饰的PHA聚合酶的核酸的方法，以及使用修饰的PHA聚合酶制备PHA的方法。本发明还包括测定改变的底物特异性的方法。

6. 发明授权

US5545545A Lysine-insensitive maize dihydrodipicolinic acid synthase 失效
标题翻译：赖氨酸不敏感玉米二氢吡啶二酸合成酶
公开(公告)号：US5545545A
公开(公告)日：1996-08-13
申请号：US53867
申请日：1993-04-27
申请人： Burle G. Gengenbach , David A. Somers , Douglas C. Bittel , Jonathan M. Shaver , Juantia M. Sellner, deceased
发明人： Burle G. Gengenbach , David A. Somers , Douglas C. Bittel , Jonathan M. Shaver , Juantia M. Sellner, deceased
IPC分类号： C12N9/88 , C12N15/29 , C12N15/82 , A01H5/00 , C07K14/415
CPC分类号： C12N15/8254 , C12N15/8209 , C12N15/8251 , C12N15/8253 , C12N9/88
摘要： A DNA sequence is provided which encodes an altered form of native Zea mays dihydrodipicolinic acid synthase (DHPS) which is substantially resistant to concentrations of L-lysine which inhibit the activity of native Zea mays DHPS, either in vitro or in transformed plant cells or whole plants.
摘要翻译：提供了一种DNA序列，其编码改变形式的天然玉米二氢二吡啶甲酸合酶（DHPS），其基本上对在体外或转化的植物细胞或整体中抑制天然Zea mays DHPS活性的L-赖氨酸的浓度具有抗性植物。

7. 发明授权

US06489462B1 Sugarcane bacilliform virus promoter 有权
标题翻译：甘蔗杆菌病毒启动子
公开(公告)号：US06489462B1
公开(公告)日：2002-12-03
申请号：US09502831
申请日：2000-02-11
申请人： Neil Olszewski , Iris Tzafrir , David A. Somers , Benham Lockhart , Kimberly A. Torbert
发明人： Neil Olszewski , Iris Tzafrir , David A. Somers , Benham Lockhart , Kimberly A. Torbert
IPC分类号： A01H100
CPC分类号： C07K14/005 , C12N15/8216 , C12N15/8222 , C12N2730/00022
摘要： An isolated and purified DNA molecule comprising a sugarcane bacilliform virus promoter and expression cassettes comprising said promoter are provided. Also provided is a method of using a sugarcane bacilliform virus promoter to express proteins, RNA transcripts, or mixtures thereof, in transgenic plants.
摘要翻译：提供了包含甘蔗杆状病毒启动子和包含所述启动子的表达盒的分离和纯化的DNA分子。还提供了在转基因植物中使用甘蔗杆状病毒启动子来表达蛋白质，RNA转录物或其混合物的方法。

8. 发明授权

US06222099B1 Transgenic plants expressing maize acetyl COA carboxylase gene and method of altering oil content 失效
标题翻译：表达玉米乙酰COA羧化酶基因的转基因植物和改变油含量的方法
公开(公告)号：US06222099B1
公开(公告)日：2001-04-24
申请号：US08930285
申请日：1998-04-13
申请人： Burle G. Gengenbach , David A. Somers , Donald L. Wyse , John W. Gronwald , Margaret A. Egli , Sheila M. Lutz
发明人： Burle G. Gengenbach , David A. Somers , Donald L. Wyse , John W. Gronwald , Margaret A. Egli , Sheila M. Lutz
IPC分类号： A01H500
CPC分类号： C12N9/93 , C12N15/8247 , C12N15/8274
摘要： The present invention provides a complete cDNA sequence and partial DNA sequences encoding maize acetyl CoA carboxylase and methods for altering the oil content of plants by introducing and expressing a maize acetyl CoA carboxylase gene in plant cells.
摘要翻译：本发明提供了完整的cDNA序列和编码玉米乙酰辅酶A羧化酶的部分DNA序列和通过在植物细胞中引入和表达玉米乙酰CoA羧化酶基因来改变植物油含量的方法。

9. 发明授权

US5498544A Method and an acetyl CoA carboxylase gene for conferring herbicide tolerance 失效
标题翻译：方法和用于赋予除草剂耐受性的乙酰CoA羧化酶基因
公开(公告)号：US5498544A
公开(公告)日：1996-03-12
申请号：US014326
申请日：1993-02-05
申请人： Burle G. Gengenbach , David A. Somers , Donald L. Wyse , John W. Gronwald , Margaret A. Egli , Sheila M. Lutz
发明人： Burle G. Gengenbach , David A. Somers , Donald L. Wyse , John W. Gronwald , Margaret A. Egli , Sheila M. Lutz
IPC分类号： A01H1/02 , A01H5/10 , C12N5/04 , C12N9/00 , C12N15/82
CPC分类号： C12N9/93 , A01H1/02 , A01H5/10 , C12N15/8247 , C12N15/8274 , C12N5/04
摘要： The present invention provides an expression cassette encoding a plant acetyl CoA carboxylase gene and methods for conferring herbicide tolerance and/or altering the oil content of plants by introducing and expressing a plant acetyl CoA carboxylase gene in plant cells. An expression cassette of the invention can comprise a gene coding a plant acetyl CoA carboxylase or a functional mutant thereof or an antisense DNA sequence complementary to the sequence for a plant acetyl CoA carboxylase gene combined with a promoter functional in a plant cell. The method of imparting herbicide tolerance to a plant includes the steps of introducing the expression cassette into the cells of a plant tissue and expressing the plant acetyl CoA carboxylase gene in an amount effective to render the acetyl CoA carboxylase and/or plant cell substantially tolerant to the herbicides. The method of altering the oil content in a plant includes the steps of introducing an expression cassette into plant cells and expressing the acetyl CoA carboxylase gene in an amount effective to alter the oil content of the cells. The expression cassette can also be introduced into a prokaryotic cell to increase yield of a plant acetyl CoA carboxylase so that crystallized enzyme can be used to screen and identify other herbicides that bind to and inhibit the enzyme.
摘要翻译：本发明提供了编码植物乙酰辅酶A羧化酶基因的表达盒和通过在植物细胞中引入和表达植物乙酰辅酶A羧化酶基因来赋予植物耐受性和/或改变植物油含量的方法。本发明的表达盒可以包含编码植物乙酰辅酶A羧化酶或其功能突变体的基因或与植物乙酰辅酶A羧化酶基因与在植物细胞中起作用的启动子组合的序列互补的反义DNA序列。赋予植物除草剂耐受性的方法包括以下步骤：将表达盒引入植物组织的细胞中，并以有效使得乙酰辅酶A羧化酶和/或植物细胞基本上耐受的量表达植物乙酰辅酶A羧化酶基因除草剂。改变植物油含量的方法包括将表达盒引入植物细胞并以有效改变细胞油含量的量表达乙酰CoA羧化酶基因的步骤。也可以将表达盒引入原核细胞以增加植物乙酰辅酶A羧化酶的产量，使得结晶的酶可用于筛选和鉴定结合并抑制酶的其它除草剂。

10. 发明授权

US5428001A Method for combating weeds among maize plants 失效
标题翻译：防治玉米杂草的方法
公开(公告)号：US5428001A
公开(公告)日：1995-06-27
申请号：US179946
申请日：1994-01-11
申请人： David A. Somers , William B. Parker , Donald L. Wyse , John W. Gronwald , Burle G. Gengenbach
发明人： David A. Somers , William B. Parker , Donald L. Wyse , John W. Gronwald , Burle G. Gengenbach
IPC分类号： A01H1/02 , A01H5/10 , C12N5/04 , C12N9/00 , C12N15/82 , A01N43/40 , A01H4/00
CPC分类号： A01H1/02 , A01H5/10 , C12N15/8247 , C12N15/8274 , C12N5/04 , C12N9/93
摘要： Maize plants are grown in a planting area that are tolerant of inhibition by a cyclohexanedione herbicide, an aryloxyphenoxypropanoic acid herbicide, or mixtures thereof, at levels which normally inhibit growth and development, wherein said tolerance is conferred by an altered acetylcoenzyme A carboxylase (ACCase) tolerant of inhibition by the herbicide at levels which normally inhibit the activity of an unaltered ACCase. Additionally, an effective quantity of the named herbicide is applied so that the weeds (e.g., grass) are killed and the maize plants are uninjured. A representative cyclohexandione herbicide is sethoxydim and a representative aryloxyphenoxypropanoic acid herbicide is haloxyfop. Competition for the maize crop posed by weeds effectively is eliminated.
摘要翻译：玉米植物在通常抑制生长和发育的水平下在耐受环己二酮除草剂，芳氧基苯氧基丙酸除草剂或其混合物的耐受性的种植区域生长，其中所述耐受性由改变的乙酰辅酶A羧化酶（ACCase）耐受除草剂在通常抑制未改变的ACCase活性的水平下的抑制作用。此外，施用有效量的命名除草剂，使得杂草（例如草）被杀死并且玉米植物未受损。一种代表性的环己二酮除草剂是稀有氧化物，代表性的芳氧基苯氧基丙酸除草剂是卤代氧氟烷。有效减少了杂草对玉米作物的竞争。

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