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1. 发明申请

US20090305342A1 Solubilization and Purification of a Target Protein fused to a Mutant Maltose-Binding Protein 有权
标题翻译：与突变体麦芽糖结合蛋白融合的靶蛋白的溶解和纯化
公开(公告)号：US20090305342A1
公开(公告)日：2009-12-10
申请号：US12297105
申请日：2007-04-14
申请人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C12P21/00 , C07K14/00 , C12N15/11 , C12N15/00 , C12N1/21 , C12N1/19
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
摘要翻译：提供了用于增加以下至少一种的方法和组合物：（i）靶蛋白与麦芽糖糊精底物的结合亲和力和/或（ii）靶蛋白质的溶解度。所述方法和组合物涉及修饰的麦芽糖结合蛋白。

2. 发明授权

US07883867B1 Solubilization and purification of a target protein fused to a mutant maltose-binding protein 有权
标题翻译：与突变体麦芽糖结合蛋白融合的目标蛋白质的溶解和纯化
公开(公告)号：US07883867B1
公开(公告)日：2011-02-08
申请号：US12857177
申请日：2010-08-16
申请人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C12N15/00 , C12N1/20 , C12P21/06
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
摘要翻译：提供了用于增加以下至少一种的方法和组合物：（i）靶蛋白与麦芽糖糊精底物的结合亲和力和/或（ii）靶蛋白质的溶解度。所述方法和组合物涉及修饰的麦芽糖结合蛋白。

3. 发明申请

US20110020911A1 SOLUBILIZATION AND PURIFICATION OF A TARGET PROTEIN FUSED TO A MUTANT MALTOSE-BINDING PROTEIN 有权
标题翻译：融合和纯化融合到突变型MALTOSE结合蛋白的目标蛋白
公开(公告)号：US20110020911A1
公开(公告)日：2011-01-27
申请号：US12857177
申请日：2010-08-16
申请人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C12N1/21 , C12N1/19 , C12N15/63 , C07H21/04
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
摘要翻译：提供了用于增加以下至少一种的方法和组合物：（i）靶蛋白与麦芽糖糊精底物的结合亲和力和/或（ii）靶蛋白质的溶解度。所述方法和组合物涉及修饰的麦芽糖结合蛋白。

4. 发明授权

US07825218B2 Solubilization and purification of a target protein fused to a mutant maltose-binding protein 有权
标题翻译：与突变体麦芽糖结合蛋白融合的目标蛋白质的溶解和纯化
公开(公告)号：US07825218B2
公开(公告)日：2010-11-02
申请号：US12297105
申请日：2007-04-14
申请人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Pei-Chung Hsieh , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C07K14/00 , C07H21/04 , C12P1/00 , C12P21/06
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided for increasing at least one of: (i) binding affinity of a target protein for a maltodextrin substrate and/or (ii) solubility of a target protein. The methods and compositions relate to a modified maltose-binding protein.
摘要翻译：提供了用于增加以下至少一种的方法和组合物：（i）靶蛋白与麦芽糖糊精底物的结合亲和力和/或（ii）靶蛋白质的溶解度。所述方法和组合物涉及修饰的麦芽糖结合蛋白。

5. 发明申请

US20120028305A1 Solubilization and Purification of a Target Protein Fused to a Mutant Maltose-Binding Protein 失效
标题翻译：融合到突变体麦芽糖结合蛋白的靶蛋白的溶解和纯化
公开(公告)号：US20120028305A1
公开(公告)日：2012-02-02
申请号：US13259837
申请日：2009-04-01
申请人： Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C12P21/02 , C12N1/21 , C12N15/63 , C07K14/00 , C07H21/04
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility, The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consist of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
摘要翻译：提供了与包含修饰的麦芽糖结合蛋白（MBP）的组合物相关的方法和组合物，当与蛋白质融合时，与蛋白质融合的野生型MBP相比，与蛋白质融合导致与麦芽糖糊精结合亲和力的增加，修饰的MBP 维持增加的溶解度。该修饰包括选自以下的突变：F68L，I318V，Q326R，V344M和T372TTTITITTTLGIEGR387，或由两个或更多个选自F68L，S146T，A313V，I318V，I318A， Q326R，V344M和T372TTTITITTTLGIEGR387突变体。

6. 发明授权

US08623615B2 Solubilization and purification of a target protein fused to a mutant maltose-binding protein 失效
标题翻译：与突变体麦芽糖结合蛋白融合的目标蛋白质的溶解和纯化
公开(公告)号：US08623615B2
公开(公告)日：2014-01-07
申请号：US13259837
申请日：2009-04-01
申请人： Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
发明人： Paul Riggs , Iris Walker , Paul A. Colussi , Mehul Ganatra , Christopher H. Taron
IPC分类号： C12P21/06 , C07K14/00 , C07H21/02
CPC分类号： C07K14/245 , C07K2319/24
摘要： Methods and compositions are provided that relate to a composition that includes a modified maltose-binding protein (MBP) which when fused to a protein results in an increase in binding affinity for maltodextrin compared with the wild type MBP fused to the protein, the modified MBP maintaining enhanced solubility. The modification includes a mutation selected from the group consisting of: F68L, I318V, Q326R, V344M, and T372TTTITITTTLGIEGR387 or consists of two or more mutations selected from the group consisting of: F68L, S146T, A313V, I318V, I318A, Q326R, V344M and T372TTTITITTTLGIEGR387 mutants.
摘要翻译：提供了与包含修饰的麦芽糖结合蛋白（MBP）的组合物相关的方法和组合物，当与蛋白质融合时，与蛋白质融合的野生型MBP相比，与蛋白质融合导致与麦芽糖糊精结合亲和力的增加，修饰的MBP 保持增强的溶解度。修饰包括选自以下的突变：F68L，I318V，Q326R，V344M和T372TTTITITTTLGIEGR387，或由两个或多个选自以下的突变组成：F68L，S146T，A313V，I318V，I318A，Q326R，V344M和 T372TTTITITTTLGIEGR387突变体。

7. 发明申请

US20050003420A1 Recycled mutagenesis of restriction endonuclease toward enhanced catalytic activity 审中-公开
标题翻译：限制性内切核酸酶的再循环诱变增强催化活性
公开(公告)号：US20050003420A1
公开(公告)日：2005-01-06
申请号：US10874527
申请日：2004-06-23
申请人： Shuang-yong Xu , Zhenyu Zhu , Paul Riggs , Pei-chung Hsieh
发明人： Shuang-yong Xu , Zhenyu Zhu , Paul Riggs , Pei-chung Hsieh
IPC分类号： C12N9/22 , C12Q1/68 , C07H21/04 , C12P21/04
CPC分类号： C12N9/22
摘要： A method is described for increasing the activity of restriction endonuclease mutants that have altered binding or cleavage activities. Restriction endonuclease variants can carry one or more amino acid substitutions that change substrate specificity and at the same time decrease the enzyme catalytic activity. A method is described for isolating derivatives of the endonuclease variants by subjecting them to additional rounds of mutagenesis and screening in a dinD::lacZ indicator strain, such that second-site mutations within the nucleotide coding sequence of the endonuclease are obtained that increased the enzyme specific activity.
摘要翻译：描述了增加具有改变的结合或切割活性的限制性内切核酸酶突变体的活性的方法。限制性内切核酸酶变体可以携带一个或多个改变底物特异性并同时降低酶催化活性的氨基酸取代。描述了通过在dinD :: lacZ指示菌株中进行额外的诱变和筛选来分离内切核酸酶变体的衍生物的方法，使得得到增加酶的核酸内切酶的核苷酸编码序列内的第二位点突变具体活动。

8. 发明申请

US20110068496A1 THERMAL CURING METHODS AND SYSTEMS FOR FORMING CONTACT LENSES 有权
标题翻译：用于形成接触透镜的热固化方法和系统
公开(公告)号：US20110068496A1
公开(公告)日：2011-03-24
申请号：US12955787
申请日：2010-11-29
申请人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
发明人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
IPC分类号： B29D11/00 , B28B5/02
CPC分类号： B29D11/00134 , B29C35/0277 , B29C35/045 , B29C37/0089 , B29C2035/048 , B29L2011/0041
摘要： Contact lens curing systems and methods are described. A contact lens curing system includes an oven that has a plurality of curing zones, a mold advancement system for moving the contact lens mold assemblies between the plurality of zones, and a controlled atmosphere within the curing zones that provides a substantially chemically inert environment in which contact lens precursor materials can be polymerized in contact lens mold assemblies located in the curing zones. Methods of producing contact lenses include curing contact lens precursor materials in contact lens mold assemblies in the lens curing system. As an example, an oven for curing contact precursor materials in molds, the curing process being done in an inert atmosphere, includes a plurality of heating zones, such as three heating zones 106, 107, 108 or two heating zones 307, 308 and a mold advancement system for moving the molds between the plurality of zones, wherein a first zone and second zone of the plurality of zones are maintained at different temperatures 106, 107 or 307, 308.
摘要翻译：描述了隐形眼镜固化系统和方法。隐形眼镜固化系统包括具有多个固化区的烘箱，用于在多个区域之间移动隐形眼镜模具组件的模具前进系统以及固化区域内的受控气氛，其提供基本上化学惰性的环境，其中隐形眼镜前体材料可以在位于固化区域的隐形眼镜模具组件中聚合。制造隐形眼镜的方法包括在透镜固化系统中的隐形眼镜模具组件中固化隐形眼镜前体材料。作为示例，用于在模具中固化接触前体材料的烘箱，固化过程在惰性气氛中进行，包括多个加热区，例如三个加热区106,107,108或两个加热区307,308和用于在多个区域之间移动模具的模具前进系统，其中多个区域中的第一区域和第二区域保持在不同的温度106,107或307,308。

9. 发明申请

US20080284049A1 THERMAL CURING METHODS AND SYSTEMS FOR FORMING CONTACT LENSES 有权
标题翻译：用于形成接触透镜的热固化方法和系统
公开(公告)号：US20080284049A1
公开(公告)日：2008-11-20
申请号：US12121404
申请日：2008-05-15
申请人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
发明人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
IPC分类号： B29D11/00
CPC分类号： B29D11/00134 , B29C35/0277 , B29C35/045 , B29C37/0089 , B29C2035/048 , B29L2011/0041
摘要： Contact lens curing systems and methods are described. A contact lens curing system includes an oven that has a plurality of curing zones a mold advancement system for moving the contact lens mold assemblies between the plurality of zones and a controlled atmosphere within the curing zones that provides a substantially chemically inert environment in which contact lens precursor materials can be polymerized in contact lens mold assemblies located in the curing zones. Methods of producing contact lenses include curing contact lens precursor materials in contact lens mold assemblies in the lens curing system. As an example, an oven for curing contact precursor materials in molds the curing process being done in an inert atmosphere, includes a plurality of heating zones, such as three heating zones 106, 107, 108 or two heating zones 307, 308 and a mold advancement system for moving the molds between the plurality of zones, wherein a first zone and second zone of the plurality of zones are maintained at different temperatures 106, 107 or 307, 308.
摘要翻译：描述了隐形眼镜固化系统和方法。隐形眼镜固化系统包括具有多个固化区的烘箱，用于在多个区域之间移动隐形眼镜模具组件的模具前进系统以及固化区域内的受控气氛，其提供基本上化学惰性的环境，其中隐形眼镜前体材料可以在位于固化区域的隐形眼镜模具组件中聚合。制造隐形眼镜的方法包括在透镜固化系统中的隐形眼镜模具组件中固化隐形眼镜前体材料。作为示例，用于在模具中固化接触前体材料的烘箱，固化过程在惰性气氛中进行，包括多个加热区，例如三个加热区106,107,108或两个加热区307,308和用于在多个区域之间移动模具的模具前进系统，其中多个区域中的第一区域和第二区域保持在不同的温度106,107或307,308。

10. 发明申请

US20120219387A1 THERMAL CURING METHODS AND SYSTEMS FOR FORMING CONTACT LENSES 有权
标题翻译：用于形成接触透镜的热固化方法和系统
公开(公告)号：US20120219387A1
公开(公告)日：2012-08-30
申请号：US13452522
申请日：2012-04-20
申请人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
发明人： Hayden Atkinson , Paul Riggs , John Gibson , Tim Warren , Ronnie Dover , Arthur Williams , Jennifer Cowden , Michael Gunner
IPC分类号： F27B9/38 , F27B9/02 , F27D3/00 , F27B9/39
CPC分类号： B29D11/00134 , B29C35/0277 , B29C35/045 , B29C37/0089 , B29C2035/048 , B29L2011/0041
摘要： Contact lens curing systems and methods are described. A contact lens curing system includes an oven that has a plurality of curing zones, a mold advancement system for moving contact lens mold assemblies between the plurality of zones, and a controlled atmosphere the curing zones that provides a substantially chemically inert environment in which contact lens precursor materials can be polymerized in contact mold assemblies located in the curing zones. Methods of producing contact lenses include curing contact lens precursor materials in contact lens mold assemblies in the lens curing system.
摘要翻译：描述了隐形眼镜固化系统和方法。隐形眼镜固化系统包括具有多个固化区的烘箱，用于在多个区域之间移动隐形眼镜模具组件的模具前进系统和可控气氛，提供基本上化学惰性的环境的固化区域，其中隐形眼镜前体材料可以在位于固化区域的接触模具组件中聚合。制造隐形眼镜的方法包括在透镜固化系统中的隐形眼镜模具组件中固化隐形眼镜前体材料。

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