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1. 发明申请

US20120040428A1 PROCEDURE FOR EXTRACTING OF LIPIDS FROM ALGAE WITHOUT CELL SACRIFICE 审中-公开
标题翻译：在没有细胞刺激的情况下从脂肪提取脂肪的程序
公开(公告)号：US20120040428A1
公开(公告)日：2012-02-16
申请号：US13207298
申请日：2011-08-10
申请人： Paul Reep , Michael Phillip Green
发明人： Paul Reep , Michael Phillip Green
IPC分类号： C12N13/00 , C12M1/42
CPC分类号： C12M47/06 , C12N13/00 , C12P7/6463
摘要： A system and method are disclosed for extracting lipids from algal cells. In the method, lipids are extracted from algal cells by exposing the algal cells in an aqueous medium to an electric field sufficient to cause release of lipids from said cells. In the system, an electric field is formed between two electrodes connected with an electrical power supply and configured such that during use an aqueous medium containing the algal cells passes between the electrodes to extract lipids therefrom.
摘要翻译：公开了从藻类细胞中提取脂质的系统和方法。在该方法中，通过将水性介质中的藻类细胞暴露于足以引起脂质从所述细胞释放的电场，从藻类细胞中提取脂质。在该系统中，在与电源连接的两个电极之间形成电场，并配置为使得在使用期间，含有藻类细胞的水性介质在电极之间通过以从其中提取脂质。

2. 发明申请

US20130211113A1 Systems and Methods for Extracting Non-Polar Lipids from an Aqueous Algae Slurry and Lipids Produced Therefrom 有权
标题翻译：从水藻类浆液中提取非极性脂质的体系和方法
公开(公告)号：US20130211113A1
公开(公告)日：2013-08-15
申请号：US13642096
申请日：2010-10-19
申请人： Nicholas Eckelberry , Michael Phillip Green , Scott Alexander Fraser
发明人： Nicholas Eckelberry , Michael Phillip Green , Scott Alexander Fraser
IPC分类号： C11B3/00
CPC分类号： C11B1/10 , A23D9/02 , B01D57/02 , B03C1/0335 , B03C1/288 , B03C1/30 , B03C2201/18 , B03D1/02 , B03D1/1462 , B03D1/24 , B03D2203/003 , C11B1/00 , C11B1/106 , C11B3/005 , C12M47/06 , C12M47/10 , C12N1/066 , C12N13/00 , C25B1/04 , Y02E60/366 , Y02W10/37
摘要： Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0.5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll.
摘要翻译：使用具有阳极和阴极的脂质提取装置来实现从微藻中提取非极性脂质的方法，系统和装置，所述脂质提取装置形成通道并限定水浆通过的流体流动路径。以0.5mm至200mm的间隙距离在通道上施加电动势，使得非极性脂质从藻类细胞中释放出来。非极性脂质可以高通量和低浓度的极性脂质例如磷脂和叶绿素提取。

3. 发明申请

US20120129244A1 SYSTEMS, METHODS AND APPARATUSES FOR DEWATERING, FLOCCULATING AND HARVESTING ALGAE CELLS 审中-公开
标题翻译：用于脱水，絮凝和收集细胞的系统，方法和装置
公开(公告)号：US20120129244A1
公开(公告)日：2012-05-24
申请号：US13274094
申请日：2011-10-14
申请人： Michael Phillip Green , Nicholas Eckelberry , Scott Fraser , Brian Goodall
发明人： Michael Phillip Green , Nicholas Eckelberry , Scott Fraser , Brian Goodall
IPC分类号： C12N1/12 , C12M1/42
CPC分类号： C11B1/02 , B03C3/0175 , C11B1/04 , C11B1/06 , C11B1/10 , C12M21/02 , C12M47/10
摘要： Described herein are systems, methods and apparatuses, for dewatering, flocculating and harvesting microorganisms. In various implementations, at least one intracellular product from the microorganisms is recovered and processed according to methods disclosed herein. In some implementations, an electromagnetic field is applied to an aqueous suspension of microorganisms thereby causing flocculation of the microorganisms. In various implementations, the microorganisms are harvested, such as by causing the microorganisms to flocculate and sink to the bottom of a container. Alternatively, the microorganisms are caused to flocculate and float to the surface of the aqueous medium where they can be recovered by skimming. In other implementations, the microorganisms are lysed as they are passed through a second electromagnetic field to release intracellular products, among other desired components of the microorganisms. In some implementations, the intracellular contents of the microorganisms, as well as cellular mass and debris, are recovered and utilized as useful products.
摘要翻译：本文描述了用于脱水，絮凝和收获微生物的系统，方法和装置。在各种实施方案中，根据本文公开的方法回收和处理来自微生物的至少一种细胞内产物。在一些实施方案中，将电磁场施加到微生物的水悬浮液，从而引起微生物的絮凝。在各种实施方案中，收获微生物，例如通过使微生物絮凝并沉降到容器的底部。或者，使微生物絮凝并漂浮到水性介质的表面，在那里它们可以通过撇去来回收。在其它实施方案中，当微生物通过第二电磁场以释放细胞内产物以及微生物的其它所需组分时，它们被裂解。在一些实施方案中，微生物的细胞内含量以及细胞质量和碎片被回收并用作有用的产物。

4. 发明授权

US09085745B2 Systems and methods for extracting non-polar lipids from an aqueous algae slurry and lipids produced therefrom 有权
标题翻译：从水性藻浆中提取非极性脂质和由其制备的脂质的系统和方法
公开(公告)号：US09085745B2
公开(公告)日：2015-07-21
申请号：US13642096
申请日：2010-10-19
申请人： Nicholas Eckelberry , Michael Phillip Green , Scott Alexander Fraser
发明人： Nicholas Eckelberry , Michael Phillip Green , Scott Alexander Fraser
IPC分类号： C11B1/00 , B01D57/00 , B01D57/02 , C02F1/40 , C11B3/00 , A23D9/02 , C11B1/10 , C12M1/00 , C12N1/06 , C12N13/00
CPC分类号： C11B1/10 , A23D9/02 , B01D57/02 , B03C1/0335 , B03C1/288 , B03C1/30 , B03C2201/18 , B03D1/02 , B03D1/1462 , B03D1/24 , B03D2203/003 , C11B1/00 , C11B1/106 , C11B3/005 , C12M47/06 , C12M47/10 , C12N1/066 , C12N13/00 , C25B1/04 , Y02E60/366 , Y02W10/37
摘要： Methods, systems, and apparatuses for extracting non-polar lipids from microalgae are achieved using a lipid extraction device having an anode and a cathode that forms a channel and defines a fluid flow path through which an aqueous slurry is passed. An electromotive force is applied across the channel at a gap distance in a range from 0.5 mm to 200 mm to cause the non-polar lipids to be released from the algae cells. The non-polar lipids can be extracted at a high throughput rate and with low concentrations of polar lipids such as phospholipids and chlorophyll.
摘要翻译：使用具有阳极和阴极的脂质提取装置来实现从微藻中提取非极性脂质的方法，系统和装置，所述脂质提取装置形成通道并限定水浆通过的流体流动路径。以0.5mm至200mm的间隙距离在通道上施加电动势，使得非极性脂质从藻类细胞中释放出来。非极性脂质可以高通量和低浓度的极性脂质例如磷脂和叶绿素提取。

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