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1. 发明授权

US5395754A Membrane-based immunoassay method 失效
标题翻译：基于膜的免疫测定法
公开(公告)号：US5395754A
公开(公告)日：1995-03-07
申请号：US923339
申请日：1992-07-31
申请人： Paul P. Lambotte , Robert C. Darter , Mark J. Sarno
发明人： Paul P. Lambotte , Robert C. Darter , Mark J. Sarno
IPC分类号： G01N33/543 , G01N33/573
CPC分类号： G01N33/54313 , G01N33/573 , Y10S435/967 , Y10S435/969 , Y10S435/97
摘要： The present invention is directed to a membrane-based immunoassay method for an analyte of interest having at least two sterically separate antigenic sites. The method comprises providing a reactive membrane having a calibration zone and a test zone, wherein the calibration zone is characterized by having a predetermined amount of the analyte of interest immobilized via a first antibody as a first specific binding pair to a solid phase, the immobilized first binding pair being covalently cross-linked such that any remaining binding sites on said first immobilized antibody are substantially incapable of further specifically binding to any additional analyte, but at least some of said analyte is capable of specifically binding to a preselected amount of a labelled second antibody. The method further includes the steps of contacting the reactive membrane with a predetermined amount of sample and allowing any analyte in the test sample to become specifically bound to immobilized first antibody in the test zone; contacting the immobilized analyte in the test and calibrator zones with a labelled second antibody capable of binding to a second antigen site on the immobilized analyte; and determining the presence or amount of analyte in the test sample by comparing the amount of labelled second antibody specifically bound in the test zone versus the amount of labelled second antibody specifically bound in the calibration zone.
摘要翻译：本发明涉及具有至少两个立体分离的抗原位点的目的分析物的基于膜的免疫测定方法。该方法包括提供具有校准区和测试区的反应性膜，其中校准区的特征在于具有通过第一抗体固定的预定量的目标分析物作为固相的第一特异性结合对，固定化第一结合对共价交联，使得所述第一固定化抗体上的任何剩余结合位点基本上不能进一步特异性结合任何其他分析物，但是所述分析物中的至少一些能够特异性结合预选量的标记第二抗体。该方法还包括以下步骤：使反应性膜与预定量的样品接触，并允许测试样品中的任何分析物与测试区中的固定的第一抗体特异性结合; 使测试和校准区中的固定化分析物与能够结合固定分析物上的第二抗原位点的标记的第二抗体接触; 以及通过比较在测试区中特异性结合的标记的第二抗体的量与在校准区中特异结合的标记的第二抗体的量来确定测试样品中分析物的存在或量。

2. 发明申请

US20130224752A1 METHOD FOR EARLY DETERMINATION OF RECURRENCE AFTER THERAPY FOR PROSTATE CANCER 审中-公开
标题翻译：早期确诊治疗前列腺癌复发的方法
公开(公告)号：US20130224752A1
公开(公告)日：2013-08-29
申请号：US13844790
申请日：2013-03-16
申请人： Robert Klem , Russ Saunders , Edward Jablonski , Thomas Adams , Mark J. Sarno
发明人： Robert Klem , Russ Saunders , Edward Jablonski , Thomas Adams , Mark J. Sarno
IPC分类号： G01N33/574
CPC分类号： G01N33/57488 , G01N33/57434 , G01N2333/96455 , G01N2800/54
摘要： This invention describes compositions and methods for use in PSA assays having low functional sensitivity which are useful, for example, in the detection of early stage recurrence of prostate disease following treatment and in the determination of whether patients have early stage biochemical reoccurrence (ES-BCR) or stable disease.
摘要翻译：本发明描述了用于具有低功能敏感性的PSA测定中的组合物和方法，其可用于例如在治疗后检测前列腺疾病的早期复发和确定患者是否具有早期生化复发（ES-BCR ）或疾病稳定。

3. 发明申请

US20120329713A1 METHOD FOR DETERMINING LIKELIHOOD OF CLINICAL RECURRENCE OR CLINICALLY STABLE DISEASE OF PROSTATE CANCER AFTER RADICAL PROSTATECTOMY 审中-公开
标题翻译：确定临床复发的临床症状或放射性前列腺癌临床稳定性疾病的方法
公开(公告)号：US20120329713A1
公开(公告)日：2012-12-27
申请号：US13489430
申请日：2012-06-05
申请人： Robert E. Klem , Russell Saunders , Edward Jablonski , Thomas H. Adams , Mark J. Sarno , Jonathan E. McDermed
发明人： Robert E. Klem , Russell Saunders , Edward Jablonski , Thomas H. Adams , Mark J. Sarno , Jonathan E. McDermed
IPC分类号： A61K38/22 , A61P35/00 , C12Q1/68
CPC分类号： G01N33/57434 , C07K16/3069 , G01N33/57488 , G01N2333/96455 , G01N2800/54
摘要： This invention describes compositions and methods for use in PSA assays having low functional sensitivity which are useful, for example, in the detection of early stage recurrence of prostate disease following treatment and in the determination of whether patients have early stage biochemical reoccurrence (ES-BCR) or stable disease.
摘要翻译：本发明描述了用于具有低功能敏感性的PSA测定中的组合物和方法，其可用于例如在治疗后检测前列腺疾病的早期复发和确定患者是否具有早期生化复发（ES-BCR ）或疾病稳定。

4. 发明申请

US20090246781A1 Method for early determination of recurrence after therapy for prostate cancer 审中-公开
标题翻译：早期确定前列腺癌治疗后复发的方法
公开(公告)号：US20090246781A1
公开(公告)日：2009-10-01
申请号：US12378965
申请日：2009-02-19
申请人： Robert Klem , Russ Saunders , Edward Jablonski , Thomas Adams , Mark J. Sarno
发明人： Robert Klem , Russ Saunders , Edward Jablonski , Thomas Adams , Mark J. Sarno
IPC分类号： C12Q1/68 , G01N33/53
CPC分类号： G01N33/57488 , G01N33/57434 , G01N2333/96455 , G01N2800/54
摘要： This invention describes compositions and methods for use in PSA assays having low functional sensitivity which are useful, for example, in the detection of early stage recurrence of prostate disease following treatment and in the determination of whether patients have early stage biochemical reoccurrence (ES-BCR) or stable disease.
摘要翻译：本发明描述了用于具有低功能敏感性的PSA测定中的组合物和方法，其可用于例如在治疗后检测前列腺疾病的早期复发和确定患者是否具有早期生化复发（ES-BCR ）或疾病稳定。

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