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1. 发明授权

US07232666B2 Optimized bacterial host strains of Methylomonas sp. 16a 失效
标题翻译：优化的细菌宿主菌株Methylomonas sp。 16a
公开(公告)号：US07232666B2
公开(公告)日：2007-06-19
申请号：US10997844
申请日：2004-11-24
申请人： Pamela L. Sharpe , Qiong Cheng , Melissa D. Bosak , Luan Tao , Natalia Sedkova
发明人： Pamela L. Sharpe , Qiong Cheng , Melissa D. Bosak , Luan Tao , Natalia Sedkova
IPC分类号： C12P23/00 , C07H21/04 , C12N9/10 , C12N15/74 , C12N1/21
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/74 , C12P23/00 , C12R1/26 , C12Y205/01029
摘要： Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, ald, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented C30 carotenoid compounds, and show an increase in the production of C40 carotenoids. The use of the optimized host strains for the production of the C40 carotenoids canthaxanthin and astaxanthin is also described.
摘要翻译：提供了通过下调类胡萝卜素生物合成途径的一个或多个crtN1，ald，crtN2和crtN3基因来优化用于生产类胡萝卜素化合物的甲烷营养型细菌菌株。所得的菌株缺乏C 30 N类胡萝卜素化合物的色素沉着，并显示出C 40 类胡萝卜素的产生增加。还描述了优化的宿主菌株用于生产C 40 H类胡萝卜素角黄素和虾青素的用途。

2. 发明授权

US06929928B2 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US06929928B2
公开(公告)日：2005-08-16
申请号：US10808807
申请日：2004-03-24
申请人： Qiong Cheng , Natalia Sedkova , Luan Tao
发明人： Qiong Cheng , Natalia Sedkova , Luan Tao
IPC分类号： C07H21/04 , C12N20060101 , C12N1/14 , C12N1/20 , C12N1/21 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/90 , C12N15/52 , C12N15/82 , C12P5/00 , C12P5/02 , C12P7/26 , C12P23/00
CPC分类号： C12N9/00 , C12N9/0004 , C12N9/001 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/825 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A unique carotenogenic biosynthetic gene cluster has been isolated from Panteoa agglomerans strain DC404, wherein the genetic organization of the cluster is crtE-idi-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：已经从成团Panthena菌株DC404中分离出独特的致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crtY-crtI-crtB-crtZ。包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ）。基因簇，基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

3. 发明授权

US07070952B2 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US07070952B2
公开(公告)日：2006-07-04
申请号：US10804677
申请日：2004-03-19
申请人： Qiong Cheng , Natalia Sedkova , Luan Tao
发明人： Qiong Cheng , Natalia Sedkova , Luan Tao
IPC分类号： C12P5/00 , C12P5/02 , C12N9/00 , C12N1/20 , C12N1/14
CPC分类号： C12N9/001 , C07H21/04 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： Genes have been isolated from Pectobacterium cypripedii encoding geranylgeranyl pyrophosphate (GGPP) synthase (CrtE), phytoene synthase (CrtB), phytoene desaturase (Crtl), lycopene cyclase (CrtY), β-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：已经从编码香叶基香叶基焦磷酸（GGPP）合成酶（CrtE），八氢番茄红素合酶（CrtB），八氢番茄红素去饱和酶（Crt1），番茄红素环化酶（CrtY），β-胡萝卜素羟化酶（CrtZ）和玉米黄质葡萄糖基转移酶（CrtX ）活动。这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

4. 发明申请

US20060035312A1 Carotenoid hydroxylase enzymes 有权
标题翻译：类胡萝卜素羟化酶
公开(公告)号：US20060035312A1
公开(公告)日：2006-02-16
申请号：US11200394
申请日：2005-08-09
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
IPC分类号： C12P23/00 , C07H21/04 , C12N9/02 , C12N1/18 , C12N15/74 , C12N1/16
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/52 , C12P23/00
摘要： A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.
摘要翻译：提供了从Brevundimonas vesicularis DC263中分离的新型CrtZ类胡萝卜素羟化酶，其可用于生产羟基化类胡萝卜素。另外，以前鉴定出来自新西伯替尼葡萄球菌的假想蛋白质已经发现具有类胡萝卜素羟化酶活性。与以前报道的其他CrtZ羟化酶相比，羟化酶基因都表现出低的同源性。异源宿主细胞中羟化酶的表达使得能够产生羟化的类胡萝卜素。

5. 发明授权

US07288387B2 Genes of strain DC413 encoding enzymes involved in biosynthesis of carotenoid compounds 失效
标题翻译：编码参与类胡萝卜素化合物生物合成的酶的DC413菌株基因
公开(公告)号：US07288387B2
公开(公告)日：2007-10-30
申请号：US10810733
申请日：2004-03-26
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
IPC分类号： C07H21/02 , C12P23/00 , C12N15/00 , C12N1/12
CPC分类号： C12N9/001 , C07H21/04 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crtX-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (CrtI), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：已经从Panteoa stewartii菌株DC413中分离出了一种类胡萝卜素生物合成基因簇，其中簇的遗传组织为crtE-idi-crtX-crtY-crtI-crtB-crtZ。包含在该簇内的基因编码er牛儿基焦磷酸（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），玉米黄质葡萄糖基转移酶（CrtX），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（CrtI），八氢番茄红素合酶（CrtB） β-胡萝卜素羟化酶（CrtZ）。基因簇，基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

6. 发明申请

US20050124033A1 Optimized bacterial host strains of methylomonas sp. 16a 失效
标题翻译：优化的甲基单胞菌属细菌宿主菌株 16a
公开(公告)号：US20050124033A1
公开(公告)日：2005-06-09
申请号：US10997844
申请日：2004-11-24
申请人： Pamela Sharpe , Qiong Cheng , Melissa Bosak , Luan Tao , Natalia Sedkova
发明人： Pamela Sharpe , Qiong Cheng , Melissa Bosak , Luan Tao , Natalia Sedkova
IPC分类号： C12N9/00 , C12N9/02 , C12N9/10 , C12N9/90 , C12N15/52 , C12P23/00 , C07H21/04 , C12N1/21 , C12N15/74
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12N15/74 , C12P23/00 , C12R1/26 , C12Y205/01029
摘要： Methanotrophic bacterial strains are provided that have been optimized for the production of carotenoid compounds through the down-regulation of one or more of the crtN1, aid, crtN2 and crtN3 genes of the carotenoid biosynthetic pathway. The resulting strains lack pigmented C30 carotenoid compounds, and show an increase in the production of C40 carotenoids. The use of the optimized host strains for the production of the C40 carotenoids canthaxanthin and astaxanthin is also described.
摘要翻译：提供了通过下调类胡萝卜素生物合成途径的一种或多种crtN1，辅助，crtN2和crtN3基因来优化用于生产类胡萝卜素化合物的甲烷营养型细菌菌株。所得的菌株缺乏C 30 N类胡萝卜素化合物的色素沉着，并显示出C 40 类胡萝卜素的产生增加。还描述了优化的宿主菌株用于生产C 40 H类胡萝卜素角黄素和虾青素的用途。

7. 发明申请

US20050014219A1 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US20050014219A1
公开(公告)日：2005-01-20
申请号：US10810733
申请日：2004-03-26
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
IPC分类号： C07H21/04 , C12N1/21 , C12N9/00 , C12N9/02 , C12N9/10 , C12N9/90 , C12N15/52 , C12N15/74 , C12P7/26 , C12P23/00
CPC分类号： C12N9/001 , C07H21/04 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00 , C12Y205/01029 , Y02E50/343
摘要： A carotenogenic biosynthetic gene cluster has been isolated from Panteoa stewartii strain DC413, wherein the genetic organization of the cluster is crtE-idi-crt)(-crtY-crtI-crtB-crtZ. The genes contained within this cluster encode geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), isopentenyl pyrophosphate isomerase (Idi), zeaxanthin glucosyl transferase (CrtX), lycopene cyclase (CrtY), phytoene desaturase (Crtl), phytoene synthase (CrtB), and β-carotene hydroxylase (CrtZ). The gene cluster, genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：从Panteoa stewartii菌株DC413中分离出一种致病基因生物合成基因簇，其中簇的遗传组织为crtE-idi-crt（-crtY-crtI-crtB-crtZ），该簇内包含的基因编码geranylgeranyl pyrophosphate（GGPP）合成酶（CrtE），异戊烯焦磷酸异构酶（Idi），玉米黄质葡萄糖基转移酶（CrtX），番茄红素环化酶（CrtY），八氢番茄红素去饱和酶（Crtl），八氢番茄红素合酶（CrtB）和β-胡萝卜素羟化酶（CrtZ）公开了基因及其产物可用于将焦磷酸法呢基转化为类胡萝卜素。含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

8. 发明授权

US07091031B2 Carotenoid hydroxylase enzymes 有权
公开(公告)号：US07091031B2
公开(公告)日：2006-08-15
申请号：US11200394
申请日：2005-08-09
申请人： Qiong Cheng , Luan Tao , Natalia Sedkova
发明人： Qiong Cheng , Luan Tao , Natalia Sedkova
IPC分类号： C12N1/21 , C12N1/13 , C12N1/15 , C12N1/19 , C12N5/10 , C12N15/63 , C12N9/02
CPC分类号： C12N9/0071 , C12N9/0004 , C12N15/52 , C12P23/00
摘要： A novel CrtZ carotenoid hydroxylase, isolated from Brevundimonas vesicularis DC263, is provided that is useful for the production of hydroxylated carotenoids. Additionally, a previously identified hypothetical protein from Novosphingobium aromaticivorans has found to have carotenoid hydroxylase activity. Both hydroxylase genes exhibit low homology in comparison to other CrtZ hydroxylases previously reported. Expression of the hydroxylases in heterologous host cells enabled production of hydroxylated carotenoids.

9. 发明授权

US07098000B2 Method for production of C30-aldehyde carotenoids 失效
标题翻译：生产C30-醛类胡萝卜素的方法
公开(公告)号：US07098000B2
公开(公告)日：2006-08-29
申请号：US10860291
申请日：2004-06-03
申请人： Qiong Cheng , Luan Tao
发明人： Qiong Cheng , Luan Tao
IPC分类号： A01H1/00 , C12N15/32 , C12N1/21 , C12Q1/68 , C07H21/04
CPC分类号： C12N9/0083 , C12N9/1085 , C12N15/52 , C12P23/00
摘要： The present invention provides methods to engineer microorganisms for the production of C30-aldehyde carotenoids. Specifically, various combinations of crtM, sqs, crtN and crtN2 genes from Staphylococcus aureus and Methylomonas sp. 16a can be co-expressed in transformant hosts, leading to the production of diaponeurosporene monoaldehyde, diapocarotene monoaldehyde, and/or diapocarotene dialdehyde. In a preferred embodiment, the genetically engineered pathway is introduced into a strain of Escherichia coli that has been engineered for the expression of carotenoids, and the C30-carotenoid product is diapocarotene dialdehyde.
摘要翻译：本发明提供了工程微生物用于生产C 30 N-醛类胡萝卜素的方法。具体来说，来自金黄色葡萄球菌和Methylomonas sp。的crtM，sqs，crtN和crtN2基因的各种组合。 16a可以在转化体宿主中共表达，导致产生二头孢噻吩一醛，二卡非罗芬单醛和/或二卡必康二醛。在优选的实施方案中，将经遗传工程改造的途径引入已被改造用于表达类胡萝卜素的大肠杆菌菌株中，并且C 30-30-类胡萝卜素产物是二卡必康二醛。

10. 发明授权

US07064196B2 Genes encoding carotenoid compounds 失效
标题翻译：编码类胡萝卜素的基因
公开(公告)号：US07064196B2
公开(公告)日：2006-06-20
申请号：US10808979
申请日：2004-03-25
申请人： Qiong Cheng , Luan Tao
发明人： Qiong Cheng , Luan Tao
IPC分类号： C12N15/31 , C12N15/54 , C12N15/55
CPC分类号： C12N9/001 , C12N9/00 , C12N9/0004 , C12N9/0071 , C12N9/0083 , C12N9/1051 , C12N9/1085 , C12N9/90 , C12N15/52 , C12P23/00
摘要： Genes have been isolated from strain DC260, a member of the Enterobacteriaceae family, encoding geranylgeranyl pyrophosphate (GGPP) synthetase (CrtE), phytoene synthase (CrtB), phytoene desaturase (CrtI), lycopene cyclase (CrtY), β-carotene hydroxylase (CrtZ), and zeaxanthin glucosyl transferase (CrtX) activity. The genes and their products are useful for the conversion of farnesyl pyrophosphate to carotenoids. Vectors containing those DNA segments, host cells containing the vectors and methods for producing those enzymes by recombinant DNA technology in transformed host organisms are disclosed.
摘要翻译：基因已经从肠杆菌科家族的成员DC260中分离出来，编码香叶基香叶基焦磷酸（GGPP）合成酶（CrtE），八氢番茄红素合酶（CrtB），八氢番茄红素去饱和酶（CrtI），番茄红素环化酶（CrtY），β-胡萝卜素羟化酶）和玉米黄质葡萄糖基转移酶（CrtX）活性。这些基因及其产物可用于将焦磷酸法呢基转化成类胡萝卜素。公开了含有这些DNA片段的载体，含有载体的宿主细胞和通过重组DNA技术在转化的宿主生物体中产生这些酶的方法。

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