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1. 发明申请

US20060189797A1 Magnetic polymer particles 审中-公开
标题翻译：磁性聚合物颗粒
公开(公告)号：US20060189797A1
公开(公告)日：2006-08-24
申请号：US10562694
申请日：2004-06-28
申请人： Pal Songe , Geir Fonnum , Nini Kjus , Marcel Sandeerg , Solveig Nordstrand , Inger Aukrust
发明人： Pal Songe , Geir Fonnum , Nini Kjus , Marcel Sandeerg , Solveig Nordstrand , Inger Aukrust
IPC分类号： C07K14/47 , C07F5/00 , B01J20/22
CPC分类号： C07K1/22 , B01D15/3828 , B01J20/28009 , B01J20/3242 , B01J20/3251 , B01J20/3265 , B01J45/00 , C08F8/00 , C08F8/42 , H01F1/42
摘要： A conjugate comprising a magnetic polymer particle bound to a carboxymethylated aspartate chelating ligand, optionally chelating a metal ion.
摘要翻译：包含与羧甲基化天冬氨酸螯合配体结合的磁性聚合物颗粒的缀合物，任选地螯合金属离子。

2. 发明申请

US20080255004A1 METHODS OF REVERSIBLY BINDING A BIOTIN COMPOUND TO A SUPPORT 审中-公开
标题翻译：将生物素化合物可逆地结合到支持物的方法
公开(公告)号：US20080255004A1
公开(公告)日：2008-10-16
申请号：US11940914
申请日：2007-11-15
申请人： Axl A. Neurauter , Lars Norderhaug , Ole Henrik Brekke , Pal Songe
发明人： Axl A. Neurauter , Lars Norderhaug , Ole Henrik Brekke , Pal Songe
IPC分类号： C40B50/18
CPC分类号： G01N33/82 , C12M23/20 , C12M47/02 , C12N13/00 , C40B50/14 , G01N33/543 , G01N33/54326
摘要： Methods of reversal of the binding between a biotin compound and a biotin-binding compound are disclosed. A method of reversibly releasing a biotinylated moiety from a streptavidin (or avidin) coated support is shown as an example. The strong interaction between streptavidin or avidin-biotin is made much weaker by using a combination of modified streptavidin or avidin and modified biotin like desthiobiotin or a derivative thereof like DSB-X Biotin. A protein, such as an antibody may be biotinylated with the modified biotin. When this protein is isolated by binding the modified biotin to the modified streptavidin or avidin bound to an solid surface, it may be released under very gently and very rapid conditions by addition of free biotin. In contrast to proteins obtained by the prior art release methods the protein obtained using the previously available release methods, the proteins obtained using the methods disclosed herein will maintain their native conformation. Uses of the methods in various procedures including cell detachment procedures and techniques of detection, identification, determination, purification, separation and/or isolation of target proteins or nucleic acid molecules are also described.
摘要翻译：公开了生物素化合物与生物素结合化合物之间的结合的逆转方法。示出了从链霉抗生物素蛋白（或抗生物素蛋白）包被的载体中可逆释放生物素化部分的方法。通过使用经修饰的链霉亲和素或抗生物素蛋白和修饰的生物素如脱硫生物素或其衍生物如DSB-X生物素的组合，链霉抗生物素蛋白或抗生物素蛋白 - 生物素之间的强相互作用变得非常弱。蛋白质，例如抗体可以用修饰的生物素生物素化。当通过将修饰的生物素结合到与固体表面结合的经修饰的链霉抗生物素蛋白或抗生物素蛋白来分离该蛋白质时，其可以通过添加游离生物素在非常温和和非常快速的条件下释放。与通过现有技术释放方法获得的蛋白质相比，使用先前可获得的释放方法获得的蛋白质，使用本文公开的方法获得的蛋白质将保持其天然构象。还描述了在各种方法中使用方法，包括细胞分离程序以及靶蛋白或核酸分子的检测，鉴定，测定，纯化，分离和/或分离技术。

3. 发明申请

US20070299249A1 Polymer Particles 审中-公开
标题翻译：聚合物颗粒
公开(公告)号：US20070299249A1
公开(公告)日：2007-12-27
申请号：US10599039
申请日：2005-03-17
申请人： Pal Songe
发明人： Pal Songe
IPC分类号： C07K1/13 , C07K14/00
CPC分类号： B01J20/26 , B01J20/265 , B01J20/28009 , B01J20/3242 , B01J20/3265 , B01J45/00
摘要： A process for covalently binding a tagged protein to a polymer particle comprising: contacting a tagged protein with a chelating agent-polymer particle conjugate wherein said tag comprises at least two histidine residues and at least two lysine residues and said chelating agent is tridentate, tetradentate or pentadentate and comprises at least two carboxyl groups and is coordinated by Co2+ ions, to form particle-chelating agent Co2+ complex: contacting said complex with a carbodiimide; and optionally removing the Co2+ ions.
摘要翻译：一种将标记蛋白质与聚合物颗粒共价结合的方法，包括：使标记的蛋白质与螯合剂 - 聚合物颗粒缀合物接触，其中所述标签包含至少两个组氨酸残基和至少两个赖氨酸残基，并且所述螯合剂是三齿的，四齿的或四戊二酸酯，并且包含至少两个羧基并且被Co 2+ +离子配位以形成颗粒螯合剂Co 2+络合物：使所述络合物与碳二亚胺接触; 并任选地除去Co 2+离子。

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