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1. 发明授权

US09758774B2 Recombinant polymerases with increased phototolerance 有权
公开(公告)号：US09758774B2
公开(公告)日：2017-09-12
申请号：US15259471
申请日：2016-09-08
申请人： Pacific Biosciences of California, Inc.
发明人： Satwik Kamtekar , Arek Bibillo , Walter Lee , Erik Miller , Insil Park
IPC分类号： C12N9/12 , C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

2. 发明申请

US20210261928A1 RECOMBINANT POLYMERASES FOR IMPROVED SINGLE MOLECULE SEQUENCING 有权
公开(公告)号：US20210261928A1
公开(公告)日：2021-08-26
申请号：US17191141
申请日：2021-03-03
申请人： Pacific Biosciences of California, Inc.
发明人： Satwik Kamtekar , Lei Jia , Robin Emig , Erik Miller , Walter H. Lee , Molly He , Insil Park
IPC分类号： C12N9/12 , C12Q1/6869 , C12P19/34
摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

3. 再颁专利

USRE47476E1 Recombinant polymerases with increased phototolerance 有权
公开(公告)号：USRE47476E1
公开(公告)日：2019-07-02
申请号：US15879240
申请日：2018-01-24
申请人： Pacific Biosciences of California, Inc.
发明人： Satwik Kamtekar , Arek Bibillo , Walter Lee , Erik Miller , Insil Park
IPC分类号： C12N9/12 , C12Q1/68 , C12P19/34 , C12Q1/6869
摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

4. 发明授权

US10717968B2 Polymerases for nucleotide analogue incorporation 有权
公开(公告)号：US10717968B2
公开(公告)日：2020-07-21
申请号：US15894157
申请日：2018-02-12
申请人： Pacific Biosciences of California, Inc.
发明人： David R. Rank , Paul S. Peluso , David K. Hanzel , Geoff Otto , Thang Pham , Fred Christians , Arekadiusz Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/6827 , C12Q1/6869
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

5. 发明授权

US09951321B2 Polymerases for nucleotide analogue incorporation 有权
公开(公告)号：US09951321B2
公开(公告)日：2018-04-24
申请号：US15370461
申请日：2016-12-06
申请人： Pacific Biosciences of California, Inc.
发明人： David K. Hanzel , Geoff Otto , Paul S. Peluso , Thang Pham , David R. Rank , Fred Christians , Arekadiusz Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogs into active site regions and for coordinating with the nucleotide analogs in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

6. 发明申请

US20160237412A1 RECOMBINANT POLYMERASES WITH INCREASED PHOTOTOLERANCE 有权
标题翻译：具有增加光敏性的重组聚合物
公开(公告)号：US20160237412A1
公开(公告)日：2016-08-18
申请号：US15049512
申请日：2016-02-22
申请人： Pacific Biosciences of California, Inc.
发明人： Satwik Kamtekar , Arek Bibillo , Walter Lee , Erik Miller , Insil Park
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252 , C12P19/34 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties include increased resistance to photodamage, and can also include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.
摘要翻译：提供了包含重组DNA聚合酶的组合物，其包括赋予聚合酶修饰性质以增强单分子测序的氨基酸取代，插入，缺失和/或外源特征。这些性质包括增加的光损伤抗性，并且还可以包括增强的金属离子配位，减少的核酸外切酶活性，在聚合酶动力学循环的一个或多个步骤中降低的反应速率，降低的支化部分，改变的辅因子选择性，增加的产率，增加的热稳定性，增加精度，提高速度，增加读长度等。还提供了编码具有上述表型的聚合酶的核酸，以及使用这种聚合酶制备DNA或顺序DNA模板的方法。

7. 发明授权

US11299720B2 Polymerases for nucleotide analogue incorporation 有权
公开(公告)号：US11299720B2
公开(公告)日：2022-04-12
申请号：US16928940
申请日：2020-07-14
申请人： Pacific Biosciences of California, Inc.
发明人： David K. Hanzel , Geoff Otto , Paul S. Peluso , Thang Pham , David R. Rank , Fred Christians , Arekadiusz Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/6827 , C12Q1/6869
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

8. 发明申请

US20170152492A1 POLYMERASES FOR NUCLEOTIDE ANALOGUE INCORPORATION 审中-公开
公开(公告)号：US20170152492A1
公开(公告)日：2017-06-01
申请号：US15370461
申请日：2016-12-06
申请人： Pacific Biosciences of California, Inc.
发明人： David K. Hanzel , Geoff Otto , Paul S. Peluso , Thang Pham , David R. Rank , Fred Christians , Arekadiusz Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12N9/12 , C12Q1/68
CPC分类号： C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.

9. 发明授权

US09556479B2 Polymerases for nucleotide analogue incorporation 有权
标题翻译：用于核苷酸类似物掺入的聚合酶
公开(公告)号：US09556479B2
公开(公告)日：2017-01-31
申请号：US14538653
申请日：2014-11-11
申请人： Pacific Biosciences of California, Inc.
发明人： David K. Hanzel , Geoff Otto , Paul Peluso , Thang Pham , David R. Rank , Paul Mitsis , Fred Christians , Arekadiusz Bibillo , Insil Park , Sonya Clark , John Lyle
IPC分类号： C12P19/34 , C12Q1/68 , C12N9/12
CPC分类号： C12N9/1252 , C07K2319/20 , C12P19/34 , C12Q1/6827 , C12Q1/6869 , C12Y207/07007 , Y02P20/52
摘要： Compositions that include polymerases with features for improving entry of nucleotide analogues into active site regions and for coordinating with the nucleotide analogues in the active site region are provided. Methods of making the polymerases and of using the polymerases in sequencing and DNA replication and amplification as well as kinetic models of polymerase activity and computer-implemented methods of using the models are also provided.
摘要翻译：提供了包含具有改善核苷酸类似物进入活性位点区域并与活性位点区域中的核苷酸类似物配位的特征的聚合酶的组合物。还提供了制备聚合酶和在测序和DNA复制和扩增中使用聚合酶的方法以及聚合酶活性的动力学模型和使用该模型的计算机实现的方法。

10. 发明申请

US20190249153A1 RECOMBINANT POLYMERASES FOR IMPROVED SINGLE MOLECULE SEQUENCING 审中-公开
公开(公告)号：US20190249153A1
公开(公告)日：2019-08-15
申请号：US16182268
申请日：2018-11-06
申请人： Pacific Biosciences of California, Inc.
发明人： Satwik Kamtekar , Lei Jia , Robin Emig , Erik Miller , Walter H. Lee , Molly He , Insil Park
IPC分类号： C12N9/12 , C12Q1/6869 , C12P19/34
CPC分类号： C12N9/1252 , C12N9/1241 , C12P19/34 , C12Q1/6869
摘要： Provided are compositions comprising recombinant DNA polymerases that include amino acid substitutions, insertions, deletions, and/or exogenous features that confer modified properties upon the polymerase for enhanced single molecule sequencing. Such properties can include enhanced metal ion coordination, reduced exonuclease activity, reduced reaction rates at one or more steps of the polymerase kinetic cycle, decreased branching fraction, altered cofactor selectivity, increased yield, increased thermostability, increased accuracy, increased speed, increased readlength, and the like. Also provided are nucleic acids which encode the polymerases with the aforementioned phenotypes, as well as methods of using such polymerases to make a DNA or to sequence a DNA template.

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