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1. 发明申请

US20100029503A1 ANALYSIS CHIP AND ANALYSIS METHOD 审中-公开
标题翻译：分析芯片和分析方法
公开(公告)号：US20100029503A1
公开(公告)日：2010-02-04
申请号：US12524058
申请日：2008-01-23
申请人： Osamu Nomura , Toshihiko Kuroda , Hitoshi Nobumasa , Yasuo Murao
发明人： Osamu Nomura , Toshihiko Kuroda , Hitoshi Nobumasa , Yasuo Murao
IPC分类号： C40B30/04 , C40B40/04
CPC分类号： G01N33/54393 , B01F13/0052 , B01F13/0059 , B01J19/0046 , B01J2219/00317 , B01J2219/00387 , B01J2219/00479 , B01J2219/00509 , B01J2219/00596 , B01J2219/00608 , B01J2219/0061 , B01J2219/00621 , B01J2219/00626 , B01J2219/00659 , B01J2219/00722 , C12Q1/6837 , C12Q2527/125 , C12Q2563/155
摘要： This invention is directed to an analysis chip comprising a substrate having a surface on which a selective binding substance is immobilized; a cover member adhered to the substrate; and particles movably contained or injected in a void between the substrate and the cover member; wherein the surfaces of the particles are coated with a surfactant. By this invention, generation of bubbles which inhibit the selective reaction between the test substance and the immobilized selective binding substance is suppressed, thereby reducing the deviation of data, suppressing the lowering of sensitivity, and promoting the reproducibility of the measurement.
摘要翻译：本发明涉及一种分析芯片，其包括具有其上固定有选择性结合物质的表面的基材; 盖构件，其粘附到所述基板; 以及可移动地容纳或注入到基板和盖构件之间的空隙中的颗粒; 其中颗粒的表面被表面活性剂涂覆。通过本发明，可以抑制抑制被检物质与固定化选择性结合物质之间的选择性反应的气泡的产生，从而减少数据的偏差，抑制灵敏度的降低，提高测定的再现性。

2. 发明授权

US09206472B2 Method for analyzing RNA 有权
标题翻译：分析RNA的方法
公开(公告)号：US09206472B2
公开(公告)日：2015-12-08
申请号：US13515377
申请日：2010-12-15
申请人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
发明人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
IPC分类号： G06F19/20 , C12Q1/68
CPC分类号： C12Q1/6851 , G06F19/20 , C12Q2565/125 , C12Q2545/101 , C12Q2525/207
摘要： A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A≦1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.
摘要翻译：一种分析从用固定剂固定的组织或细胞提取的RNA的方法，所述方法包括确定步骤，所述确定步骤是否满足以下条件：B / A＆nlE; 1其中A表示RNA在该范围内的重量比（％）相对于通过电泳确定的RNA的总重量为1000至4000个核苷酸; B表示通过电泳测定的相对于RNA总重量超过4000个核苷酸的RNA的重量比（％）。

3. 发明申请

US20120196768A1 METHOD FOR PREPARING aRNA AND METHOD FOR ANALYSIS OF GENE EXPRESSION 审中-公开
标题翻译：制备aRNA的方法和基因表达分析方法
公开(公告)号：US20120196768A1
公开(公告)日：2012-08-02
申请号：US13499824
申请日：2011-04-21
申请人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
发明人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
IPC分类号： C40B30/04 , C12P19/34
CPC分类号： C12N15/1003 , C12N15/1096 , C12Q1/6827 , C12Q1/6865 , C12Q2527/137
摘要： A method for preparing aRNA to be used for gene expression analysis from an RNA sample extracted from a tissue or cell(s) fixed with a fixative includes an amplification step of the RNA sample by reverse transcription and in vitro transcription, the ratio of aminoallyl uridine 5′-triphosphate (AA-UTP) in a nucleotide reagent used in the in vitro transcription is not less than 5 mol % and less than 25 mol % with respect to the total of uridine 5′-triphosphate (UTP) and AA-UTP.
摘要翻译：从用固定剂固定的组织或细胞提取的RNA样品制备用于基因表达分析的RNA的方法包括通过逆转录和体外转录的RNA样品的扩增步骤，氨基烯丙尿苷的比例用于体外转录的核苷酸试剂中的5'-三磷酸（AA-UTP）相对于尿苷5'-三磷酸（UTP）和AA-UTP的总量不低于5mol％且小于25mol％。

4. 发明申请

US20120253687A1 METHOD FOR ANALYZING RNA 有权
标题翻译：分析RNA的方法
公开(公告)号：US20120253687A1
公开(公告)日：2012-10-04
申请号：US13515377
申请日：2010-12-15
申请人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
发明人： Toshihiko Kuroda , Osamu Nomura , Hitoshi Nobumasa
IPC分类号： G06F19/00
CPC分类号： C12Q1/6851 , G06F19/20 , C12Q2565/125 , C12Q2545/101 , C12Q2525/207
摘要： A method of analyzing RNA extracted from a tissue or cell(s) fixed with a fixative, said method comprising a determining step of whether said RNA satisfies: B/A≦1 wherein A represents the weight ratio (%) of RNA within the range of 1000 to 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis; and B represents the weight ratio (%) of RNA within the range of more than 4000 nucleotides with respect to the total weight of RNA as determined by electrophoresis.
摘要翻译：一种分析从用固定剂固定的组织或细胞提取的RNA的方法，所述方法包括确定步骤，所述确定步骤是否满足以下条件：B / A＆nlE; 1其中A表示RNA在该范围内的重量比（％）相对于通过电泳确定的RNA的总重量为1000至4000个核苷酸; B表示通过电泳测定的相对于RNA总重量超过4000个核苷酸的RNA的重量比（％）。

5. 发明授权

US07795010B2 Fractionator and method of fractionation 有权
$Fractionator and method of fractionation$
标题翻译：分馏器和分馏方法
公开(公告)号：US07795010B2
公开(公告)日：2010-09-14
申请号：US10586678
申请日：2005-01-20
申请人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
发明人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
IPC分类号： C12M3/00
CPC分类号： B01D63/04 , B01D61/142 , B01D61/20 , B01D63/08 , B01D2313/243 , B01D2319/022 , C07K1/22 , C07K1/34 , C07K1/36
摘要： The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.
摘要翻译：以下公开了：（1）包括过滤部分，浓缩部分，回收部分和液体供给泵的膜分馏器，其中将过滤部分，浓缩部分和回收部分彼此连接的流动通道构成闭合电路 ; （2）一种生物成分分离方法，其特征在于，将来自生物成分的样品加入抗体吸附膜分离系统中，所述抗体吸附膜分离系统具有能够吸附在膜分离系统的中部或后部内部容纳的特定蛋白质的抗体，不存在吸附蛋白质的抗体，人α1-微球蛋白与人白蛋白之间的渗透比为1.5〜1000，从而分离部分生物成分; （3）蛋白质分级法的方法，其特征在于，使含有2种以上蛋白质和水的溶液与中空丝分离膜接触，从而进行蛋白质分馏，其特征在于，分离液含有有机溶剂。

6. 发明申请

US20070244306A1 Fractionator and Method of Fractionation 有权
标题翻译：分馏器和分馏方法
公开(公告)号：US20070244306A1
公开(公告)日：2007-10-18
申请号：US10586678
申请日：2005-01-20
申请人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
发明人： Kazuhiro Tanahashi , Ichiro Kumo , Nobuyuki Kuroki , Hiroyuki Sugaya , Satoko Yamada , Shigehisa Wada , Giman Jung , Toshihiko Kuroda , Shuji Sekiguchi
IPC分类号： C07K1/34 , B01D37/00 , B01D63/00
CPC分类号： B01D63/04 , B01D61/142 , B01D61/20 , B01D63/08 , B01D2313/243 , B01D2319/022 , C07K1/22 , C07K1/34 , C07K1/36
摘要： The following is disclosed: (1) a membrane fractionator including a filtration section, a concentrating section, a recovery section and a liquid feed pump, wherein a flow channel connecting the filtration section, concentrating section and recovery section to each other constitutes a closed circuitry; (2) a method of biocomponent separation, characterized in that a sample derived from biocomponents is fed into an antibody adsorption membrane separation system having an antibody capable of adsorbing specified protein internally accommodated in the middle or a rear part of a membrane separation system that in the absence of antibodies adsorbing proteins, exhibits a permeation ratio between human alpha1-microglobulin and human albumin of 1.5 to 1000, thereby separating part of the biocomponents; and (3) a method of protein fractionation, comprising bringing a solution containing two or more types of proteins and water into contact with a hollow yarn separation membrane to thereby attain protein fractionation, characterized in that the fractionation solution contains an organic solvent.
摘要翻译：以下公开了：（1）包括过滤部分，浓缩部分，回收部分和液体供给泵的膜分馏器，其中将过滤部分，浓缩部分和回收部分彼此连接的流动通道构成闭合电路 ; （2）一种生物成分分离方法，其特征在于，将来自生物成分的样品加入抗体吸附膜分离系统中，所述抗体吸附膜分离系统具有能够吸附在膜分离系统的中部或后部内部容纳的特定蛋白质的抗体，不存在吸附蛋白质的抗体，人α1-微球蛋白与人白蛋白之间的渗透比为1.5〜1000，从而分离部分生物成分; （3）蛋白质分级法的方法，其特征在于，使含有2种以上蛋白质和水的溶液与中空丝分离膜接触，从而进行蛋白质分馏，其特征在于，分离液含有有机溶剂。

7. 发明授权

US4363005A Power conserving blocking oscillator power supply circuit 失效
标题翻译：省电阻塞振荡器电源电路
公开(公告)号：US4363005A
公开(公告)日：1982-12-07
申请号：US243941
申请日：1981-02-27
申请人： Toshihiko Kuroda , Kazuo Sugiyama
发明人： Toshihiko Kuroda , Kazuo Sugiyama
IPC分类号： H02M3/338 , H03K3/012 , H03K3/30 , G05F1/64 , H02M3/335
CPC分类号： H03K3/012 , H02M3/338 , H03K3/30
摘要： A power source circuit for a discharge type sensing circuit, comprising a blocking oscillator (1 ) and a feedback circuit (2) for changing the period of oscillation of the blocking oscillator (1). The sensing circuit as a load is connected to a secondary winding (L.sub.2) of a transformer (T) of the blocking oscillator (1). The base bias of a transistor (Tr.sub.1) of the blocking oscillator is controlled by the feedback circuit (2) to which is applied a negative voltage produced in the primary winding (L.sub.1) in an off state of the transistor (Tr.sub.1). As a result, when the sensing circuit is in the sensing mode of operation, the period of oscillation becomes shorter for rapid supply of electric power and, when in the waiting mode of operation, the period of oscillation becomes longer for reducing supply of power, so that the power consumption is reduced as a whole.
摘要翻译： PCT No.PCT / JP79 / 00176 Sec。 371日期1981年2月27日 102（e）日期1981年2月27日PCT提交1979年7月3日PCT公布。公开号WO81 / 00178 日期：1981年1月22日。一种用于放电型感测电路的电源电路，包括用于改变阻塞振荡器（1）的振荡周期的阻塞振荡器（1）和反馈电路（2）。作为负载的感测电路连接到阻塞振荡器（1）的变压器（T）的次级绕组（L2）。阻塞振荡器的晶体管（Tr1）的基极偏置由在晶体管（Tr1）的截止状态下施加在初级绕组（L1）中产生的负电压的反馈电路（2）控制。结果，当感测电路处于感测操作模式时，快速电力供应的振荡周期变短，并且当处于等待操作模式时，振荡周期变得更长以减少电力供应，从而整体上降低了功耗。

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