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1. 发明授权

US08673609B2 Buttiauxella sp. phytase variants 有权
标题翻译： Buttiauxella sp。植酸酶变体
公开(公告)号：US08673609B2
公开(公告)日：2014-03-18
申请号：US12988363
申请日：2009-04-17
申请人： Oliver Kensch , Klaus Schulze Pellengahr , Birgitta Leuthner , Jayarama K. Shetty , Michael Pepsin , Søren Dalsgaard , Mai Faurschou Isaksen
发明人： Oliver Kensch , Klaus Schulze Pellengahr , Birgitta Leuthner , Jayarama K. Shetty , Michael Pepsin , Søren Dalsgaard , Mai Faurschou Isaksen
IPC分类号： C12N9/16 , C07K14/00 , C12Q1/44 , C12P21/00
CPC分类号： A23K20/189 , A23L29/06 , A23V2002/00 , C12N9/16 , C12P7/06 , C12P19/02 , C12P19/14 , C12Y301/03026 , Y02E50/17
摘要： Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.
摘要翻译：本文提供了Buttiauxella sp。可用于工业应用的植酸酶，包括淀粉液化，酒精发酵和增强食品和动物饲料中磷酸盐消化的方法。

2. 发明申请

US20110201081A1 Buttiauxella Sp. Phytase Variants 有权
标题翻译： Buttiauxella Sp。植酸酶变体
公开(公告)号：US20110201081A1
公开(公告)日：2011-08-18
申请号：US12988363
申请日：2009-04-17
申请人： Oliver Kensch , Klaus Schulze Pellengahr , Birgitta Leuthner , Jayarama K. Shetty , Michael Pepsin , Søren Dalsgaard , Mai Faurschou Isaksen
发明人： Oliver Kensch , Klaus Schulze Pellengahr , Birgitta Leuthner , Jayarama K. Shetty , Michael Pepsin , Søren Dalsgaard , Mai Faurschou Isaksen
IPC分类号： C12N9/02 , C12N9/16 , C07H21/00 , C12N15/63 , C12N5/10 , C12N9/20 , C12N9/10
CPC分类号： A23K20/189 , A23L29/06 , A23V2002/00 , C12N9/16 , C12P7/06 , C12P19/02 , C12P19/14 , C12Y301/03026 , Y02E50/17
摘要： Provided herein are variants of Buttiauxella sp. phytases that may be used in industrial applications including methods for starch liquefaction, alcohol fermentations and for enhancing phosphate digestion in foods and animal feeds.
摘要翻译：本文提供了Buttiauxella sp。可用于工业应用的植酸酶，包括淀粉液化，酒精发酵和增强食品和动物饲料中磷酸盐消化的方法。

3. 发明申请

US20120196002A1 METHOD OF PRODUCING A LIPOLYTIC ENZYME 有权
标题翻译：生产脂肪酶的方法
公开(公告)号：US20120196002A1
公开(公告)日：2012-08-02
申请号：US13265712
申请日：2010-04-23
申请人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
发明人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
IPC分类号： A23L1/48 , C12N1/15 , C12N9/20
CPC分类号： C12N9/20 , A23K20/189 , A23L29/06 , A23L33/14 , A23V2002/00 , C12N15/80 , C12Y301/01003
摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of said heterologous nucleotide sequence(s) encoding said lipolytic enzyme.
摘要翻译：本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码所述脂肪分解酶的所述异源核苷酸序列的条件下培养细胞。

4. 发明申请

US20140322727A1 ASSAY 审中-公开
标题翻译：测定
公开(公告)号：US20140322727A1
公开(公告)日：2014-10-30
申请号：US14131144
申请日：2012-06-22
申请人： Mai Faurschou Isaksen , Anja Hemmingsen Kellett-Smith , Neil McLennan , Ceinwen Gilbert , Majbritt Hauge Kyneb
发明人： Mai Faurschou Isaksen , Anja Hemmingsen Kellett-Smith , Neil McLennan , Ceinwen Gilbert , Majbritt Hauge Kyneb
IPC分类号： G01N33/573
CPC分类号： G01N33/573 , C12Q1/00 , C12Q1/34 , C12Q1/42 , G01N33/558 , G01N2333/914 , G01N2333/916 , G01N2333/924
摘要： The present invention discloses an assay device (1) for detecting active enzyme in a sample. Said assay device (1) comprises the following components:—(a) a placement region (10) onto which the sample can be placed; (b) a matrix (20) operably connected to said placement region (10) such that the sample when present (such as placed) on said placement region (10) can migrate along said matrix (20); (c) at least one distinct capture location (30) on said matrix (20), wherein each distinct capture location (30) is distanced away from the placement region (10), and wherein the sample can migrate across said distinct capture location (30); (d) capture means (40) being present at or defining each distinct capture location (30), wherein said capture means (40) are capable of binding to said enzyme such that at least a portion of said sample of said enzyme is retained at at least one distinct capture location (30); and (e) selective indication means (50), or at least a component thereof, to provide selective indication of the presence of active enzyme bound to said capture means (40).
摘要翻译：本发明公开了一种用于检测样品中活性酶的测定装置（1）。所述测定装置（1）包括以下组件： - （a）放置样品的放置区域（10）; （b）可操作地连接到所述放置区域（10）的矩阵（20），使得当所述放置区域（10）上存在的样品（例如放置）可以沿所述基质（20）迁移; （c）所述矩阵（20）上的至少一个不同的捕获位置（30），其中每个不同的捕获位置（30）远离所述放置区域（10）远离，并且其中所述样品可以迁移穿过所述不同的捕获位置 30）; （d）存在于或限定每个不同捕获位置（30）的捕获装置（40），其中所述捕获装置（40）能够结合所述酶，使得所述酶的所述样品的至少一部分保留在至少一个不同的捕获位置（30）; （e）选择性指示装置（50）或其至少一个组分，以提供与所述捕获装置（40）结合的活性酶的存在的选择性指示。

5. 发明申请

US20130171296A1 METHOD 审中-公开
标题翻译：方法
公开(公告)号：US20130171296A1
公开(公告)日：2013-07-04
申请号：US13808884
申请日：2011-07-07
申请人： Mai Faurschou Isaksen
发明人： Mai Faurschou Isaksen
IPC分类号： C12Q1/40 , A23K1/165
CPC分类号： C12N9/2417 , A23K20/174 , A23K20/189 , A23K20/20 , A23K40/10 , A23K40/25 , A23K40/30 , A23K50/30 , A23K50/75 , A23K50/80 , C07K2319/02 , C12Q1/40 , C12Y302/01001 , Y02A40/818 , Y02P60/877
摘要： The present invention relates to a method for identifying a pepsin resistant alpha amylase enzyme for use in a feed supplement comprising: i) providing an alpha amylase enzyme; ii) admixing said alpha amylase with corn based feed and buffer solution comprising a pepsin concentration of 9000 U/ml at pH 3, 40° C., 500 rpm for at least 120 minutes and analysing alpha amylase activity on said alpha amylase compared to a control sample; wherein said control sample differs in that no pepsin is present during incubation; and iii) selecting an alpha amylase enzyme which substantially maintains alpha amylase activity under the assay conditions; feed supplements and feedstuffs comprising a pepsin resistant alpha amylase and the use of pepsin resistant alpha amylases in feed.
摘要翻译：本发明涉及用于鉴定用于饲料添加物的胃蛋白酶抗性α淀粉酶的方法，其包括：i）提供α淀粉酶; ii）将所述α淀粉酶与基于玉米的饲料和缓冲溶液混合，所述饲料和缓冲溶液在pH 3，40℃，500rpm下包含9000U / ml的胃蛋白酶浓度至少120分钟，并分析所述α淀粉酶上的α淀粉酶活性，与对照样品; 其中所述对照样品的差异在于孵育期间不存在胃蛋白酶; 和iii）选择在测定条件下基本上保持α-淀粉酶活性的α淀粉酶; 饲料添加剂和饲料，其包含胃蛋白酶抗性α淀粉酶和在饲料中使用胃蛋白酶抗性α淀粉酶。

6. 发明申请

US20120107296A1 FEED SUPPLEMENT 有权
标题翻译：饲料补充
公开(公告)号：US20120107296A1
公开(公告)日：2012-05-03
申请号：US13265725
申请日：2010-04-23
申请人： Mai Faurschou Isaksen , Rikke Høegh Lorentsen , Peter Plumstead , Luis Fernando Romero Millán , Susan Madrid , Cherry Lin , Michael Ward , Masoud Rajabi Zargahi
发明人： Mai Faurschou Isaksen , Rikke Høegh Lorentsen , Peter Plumstead , Luis Fernando Romero Millán , Susan Madrid , Cherry Lin , Michael Ward , Masoud Rajabi Zargahi
IPC分类号： A61K38/54 , A61P3/02 , A61P1/00
CPC分类号： A23L1/30 , A23K20/189 , A23K50/30 , A23K50/75 , A23L33/10 , A61K38/465 , C12N9/16 , C12N9/20
摘要： The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.
摘要翻译：本发明涉及包含植酸酶和脂肪分解酶的饲料添加剂，其中所述脂肪分解酶在约pH1.5至约pH3.5的pH范围内具有脂肪酶活性。

7. 发明授权

US08940519B2 Method of producing a lipolytic enzyme 有权
标题翻译：产生脂肪分解酶的方法
公开(公告)号：US08940519B2
公开(公告)日：2015-01-27
申请号：US13265712
申请日：2010-04-23
申请人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
发明人： Susan Madrid , Cherry Lin , Masoud Rajabi Zargahi , Rikke Høegh Lorentsen , Mai Faurschou Isaksen , Michael Ward
IPC分类号： C12N1/20 , C12N9/20 , C12N15/00 , A23L1/03 , A23K1/165 , C12N15/80
CPC分类号： C12N9/20 , A23K20/189 , A23L29/06 , A23L33/14 , A23V2002/00 , C12N15/80 , C12Y301/01003
摘要： The present invention provides a transformed or transfected Trichoderma reesei cell comprising one or more of: (i) at least one heterologous nucleotide sequence encoding a lipolytic enzyme comprising an amino acid sequence shown as SEQ ID NO: 1 or SEQ ID NO:2, (ii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence shown as SEQ ID NO: 3 or SEQ ID NO: 4, or (iii) at least one heterologous nucleotide sequence encoding a lipolytic enzyme wherein the nucleotide sequence comprises the nucleotide sequence which hybridizes to SEQ ID NO: 3 or SEQ ID NO: 4 or a nucleotide sequence which is at least 40% sequence identity to SEQ ID NO: 3 or SEQ ID NO: 4; and culturing the cell under conditions to allow for expression of the heterologous nucleotide sequence(s) encoding the lipolytic enzyme.
摘要翻译：本发明提供转化或转染的里氏木霉细胞，其包含以下一种或多种：（i）至少一种编码脂解酶的异源核苷酸序列，其包含SEQ ID NO：1或SEQ ID NO：2所示的氨基酸序列（ ii）至少一个编码脂解酶的异源核苷酸序列，其中所述核苷酸序列包含如SEQ ID NO：3或SEQ ID NO：4所示的核苷酸序列，或（iii）至少一个编码脂解酶的异源核苷酸序列，其中核苷酸序列包含与SEQ ID NO：3或SEQ ID NO：4杂交的核苷酸序列或与SEQ ID NO：3或SEQ ID NO：4具有至少40％序列同一性的核苷酸序列; 并在允许表达编码脂肪分解酶的异源核苷酸序列的条件下培养细胞。

8. 发明授权

US08445250B2 Feed supplement 有权
标题翻译：饲料补充
公开(公告)号：US08445250B2
公开(公告)日：2013-05-21
申请号：US13265725
申请日：2010-04-23
申请人： Mai Faurschou Isaksen , Rikke Høegh Lorentsen , Peter Plumstead , Luis Fernando Romero Millán , Susan Madrid , Cherry Lin , Michael Ward , Masoud Rajabi Zargahi
发明人： Mai Faurschou Isaksen , Rikke Høegh Lorentsen , Peter Plumstead , Luis Fernando Romero Millán , Susan Madrid , Cherry Lin , Michael Ward , Masoud Rajabi Zargahi
IPC分类号： C12N9/14
CPC分类号： A23L1/30 , A23K20/189 , A23K50/30 , A23K50/75 , A23L33/10 , A61K38/465 , C12N9/16 , C12N9/20
摘要： The present invention relates to a feed supplement comprising a phytase and a lipolytic enzyme, wherein said lipolytic enzyme has lipase activity at a pH in the range of about pH1.5 to about pH3.5.
摘要翻译：本发明涉及包含植酸酶和脂肪分解酶的饲料添加剂，其中所述脂肪分解酶在约pH1.5至约pH3.5的pH范围内具有脂肪酶活性。

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