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1. 发明申请

US20130078737A1 THYROXINE IMMUNOASSAY USING FLUORESCENT PARTICLES 有权
标题翻译：使用荧光颗粒进行甲状腺素免疫
公开(公告)号：US20130078737A1
公开(公告)日：2013-03-28
申请号：US13610497
申请日：2012-09-11
申请人： Noriyuki KASAGI , Junichi KATADA , Tadahiro MATSUNO
发明人： Noriyuki KASAGI , Junichi KATADA , Tadahiro MATSUNO
IPC分类号： G01N33/78 , G01N21/64
CPC分类号： G01N33/54373 , G01N33/54306 , G01N33/585 , G01N33/78
摘要： Provided are a base plate and a method for thyroxine immunoassay, which are capable of decreasing the signal change rate caused by temperature change in a high-sensitivity immunoassay method for thyroxine. A base plate for thyroxine immunoassay which has a conjugate of thyroxine or a derivative thereof and albumin immobilized thereon, and in which the ratio of the molecule number of thyroxine or a derivative thereof to the molecule number of albumin in the immobilized conjugate, is disclosed.
摘要翻译：本发明提供甲状腺素免疫测定法的基板和甲基荧光素免疫测定方法，能够降低甲状腺素的高灵敏度免疫测定法中的温度变化引起的信号变化率。公开了一种甲状腺素免疫测定的基板，其具有甲状腺素或其衍生物和固定在其上的白蛋白的缀合物，其中甲状腺素或其衍生物的分子数与固定化缀合物中白蛋白的分子数之比。

2. 发明申请

US20120322165A1 HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHY METHOD AND IMMUNOCHROMATOGRAPHY KIT 有权
标题翻译：高敏感性免疫印迹法和免疫印迹试剂盒
公开(公告)号：US20120322165A1
公开(公告)日：2012-12-20
申请号：US13523388
申请日：2012-06-14
申请人： Junichi KATADA , Hiroyuki CHIKU
发明人： Junichi KATADA , Hiroyuki CHIKU
IPC分类号： G01N33/53
CPC分类号： G01N33/54393 , G01N33/5308
摘要： The present invention is to provide an immunochromatography method which is capable of performing a detection with high-sensitivity or reduced false-positives by suppressing the occurrence of false-positive when a signal is amplified. An immunochromatography method includes in a state of forming a complex from a test substance and a labeling substance containing a metal modified with a first binding substance for the test substance, developing the complex on an insoluble carrier in presence of a protease hydrolyzate of protein; capturing the test substance and the labeling substance in a detection site on the insoluble carrier containing a substance which has a binding property to a second binding substance for the test substance or the first binding substance for the test substance; and detecting the test substance by amplifying the labeling substance captured.
摘要翻译：本发明提供一种免疫色谱法，其能够通过抑制信号放大时的假阳性发生而通过高灵敏度或降低的假阳性进行检测。免疫色谱法包括形成来自测试物质的复合物和含有用于测试物质的第一结合物质修饰的金属的标记物质，在蛋白质蛋白酶水解产物存在下，在不溶性载体上形成复合物; 在检测部位的不溶性载体上捕获检测物质和标记物质，所述不溶性载体含有与被检物质的第二结合物质或测试物质的第一结合物质具有结合性的物质; 并通过放大捕获的标记物质来检测被检物质。

3. 发明申请

US20090203147A1 BLOTTING DETECTION METHOD 审中-公开
标题翻译： BLOTTING检测方法
公开(公告)号：US20090203147A1
公开(公告)日：2009-08-13
申请号：US12369518
申请日：2009-02-11
申请人： Junichi KATADA
发明人： Junichi KATADA
IPC分类号： G01N33/20
CPC分类号： G01N33/54306 , G01N33/585
摘要： It is an object of the present invention to provide a blotting detection method capable of quick detection of a very tiny amount of an analysis target. The present invention provides a blotting detection method which comprises moving an analyte held on a first carrier with a developing solution, and adsorbing the analyte onto a second carrier, wherein the analyte is labeled with metal fine particle and is detected by sensitization with use of a silver-containing compound and a reducing agent for silver ion, and the labeling substance having a size of not less than 1 μm and not more than 20 μm in the average particle size at the time of detection is detected.
摘要翻译：本发明的目的是提供能够快速检测非常小量的分析对象的印迹检测方法。本发明提供了一种印迹检测方法，其包括使用显影液移动保持在第一载体上的分析物，并将分析物吸附到第二载体上，其中分析物用金属微粒标记，并通过使用含银化合物和银离子还原剂，检测出平均粒径为1μm以上20μm以下的标记物质。

4. 发明申请

US20120252004A1 HIGHLY SENSITIVE IMMUNOCHROMATOGRAPHY METHOD 审中-公开
标题翻译：高敏感免疫印迹法
公开(公告)号：US20120252004A1
公开(公告)日：2012-10-04
申请号：US13419153
申请日：2012-03-13
申请人： Atsuhiko WADA , Junichi KATADA
发明人： Atsuhiko WADA , Junichi KATADA
IPC分类号： G01N30/00
CPC分类号： G01N33/558
摘要： Provided is an immunochromatography method that enables highly sensitive detection by reducing the background density of a non-measurement site in the immunochromatography method. The immunochromatography method includes developing a complex of a substance to be tested and a labeling substance containing a metal modified with a first binding substance for the substance to be tested on an insoluble carrier in the presence of a surfactant having a steroid skeleton while the substance to be tested and the labeling substance form the complex; and detecting the complex of the substance to be tested by capturing the substance to be tested and the labeling substance on a detection site of the insoluble carrier containing a second binding substance for the substance to be tested or a substance that can bind to the first binding substance for the substance to be tested.
摘要翻译：提供了一种免疫色谱法，其通过在免疫色谱法中降低非测量位点的背景密度来实现高度灵敏的检测。免疫色谱方法包括在具有类固醇骨架的表面活性剂的存在下，将待测物质的复合物和含有用待测物质的第一结合物修饰的金属在不溶性载体上开发的物质与被测试，标签物质形成复合物; 以及通过在被检物质中含有第二结合物质的不溶性载体的检测部位或能够与第一结合物结合的物质捕获待测试物质和标记物质来检测待测物质的复合物待测物质的物质。

5. 发明申请

US20110072885A1 CHROMATOGRAPHIC MEASUREMENT APPARATUS 有权
标题翻译：色谱测量装置
公开(公告)号：US20110072885A1
公开(公告)日：2011-03-31
申请号：US12895003
申请日：2010-09-30
申请人： Katsuya INANA , Tomonori NISHIO , Junichi KATADA
发明人： Katsuya INANA , Tomonori NISHIO , Junichi KATADA
IPC分类号： G01N30/02
CPC分类号： G01N21/78 , G01N21/8483 , G01N30/95
摘要： A chromatographic measurement apparatus for measuring a color development state of an insoluble carrier with a sample solution and a label solution developed thereon to test a test article, the insoluble carrier including a test detection area where a material that binds specifically to a test article is immobilized and a control detection area used for determining an end of measurement. The chromatographic measurement apparatus includes: a determining unit for carrying out determination of validity and determination of necessity of amplification of a test result of the test article; and an amplifying unit for amplifying the color development state according to the result of the determination of necessity of amplification by the determining unit. The amplification is carried out only when it is determined that amplification is necessary.
摘要翻译：一种用于测量不溶性载体的显色状态的色谱测量装置，其中样品溶液和其上形成的标记溶液在其上显影以测试测试制品，所述不溶性载体包括测试检测区域，其中固定有特异性地与测试制品结合的材料以及用于确定测量结束的控制检测区域。色谱测量装置包括：确定单元，用于确定测试物品的测试结果的有效性和放大必要性的确定; 以及放大单元，用于根据由确定单元确定放大的必要性的结果来放大显色状态。只有当确定需要扩增时才进行扩增。

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