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    • 1. 发明申请
    • CONTINUOUS PRODUCTION OF BIODIESEL FUEL BY ENZYMATIC METHOD
    • 通过酶联免疫法连续生产生物柴油
    • US20100261235A1
    • 2010-10-14
    • US12745962
    • 2008-11-28
    • Nobuyuki KurataniShinji HamaHideo NodaHideki Fukuda
    • Nobuyuki KurataniShinji HamaHideo NodaHideki Fukuda
    • C12P7/64C12M1/02
    • C12P7/649C10G2300/1011C11C3/003C12P7/62Y02E50/13Y02P30/20
    • A method for continuously producing a fatty acid ester of the present invention comprises (a) mixing and agitating an oil and fat starting material and a lower alcohol, and supplying a mixture to one of the catalyst reaction tubes filled with a lipase; (b) producing a fatty acid ester and glycerin in the catalyst reaction tube; (c) introducing an outflowing liquid from the catalyst reaction tube into a glycerin separation tank, thereby collecting the glycerin; (d) adding a lower alcohol to a separated liquid obtained by separating the glycerin from the outflowing liquid, mixing and agitating an obtained material, and supplying a mixture to a following catalyst reaction tube; (e) repeating the steps (b) to (d) until supply to a last catalyst reaction tube is performed; and (f) collecting a fatty acid ester from the separated liquid obtained from the last catalyst reaction tube. According to the method of the present invention, the concentration of a lower alcohol can be strictly controlled and by product glycerin can be automatically removed.
    • 本发明的连续生产脂肪酸酯的方法包括:(a)将油脂原料和低级醇混合搅拌,向填充有脂肪酶的催化剂反应管之一供给混合物; (b)在催化剂反应管中制备脂肪酸酯和甘油; (c)将来自催化剂反应管的流出液引入甘油分离槽中,从而收集甘油; (d)向从流出液中分离甘油得到的分离液中加入低级醇,混合搅拌所得物质,向后述催化剂反应管供给混合物; (e)重复步骤(b)至(d),直到进行最后一个催化剂反应管的供应; 和(f)从最后一个催化剂反应管获得的分离液中收集脂肪酸酯。 根据本发明的方法,可以严格控制低级醇的浓度,并且可以自动除去产物甘油。