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1. 发明授权

US5151268A Methods of using recombinant human protein C 失效
标题翻译：使用重组人蛋白C的方法
公开(公告)号：US5151268A
公开(公告)日：1992-09-29
申请号：US215112
申请日：1988-07-05
申请人： Nils U. Bang , Robert J. Beckmann , S. Richard Jaskunas , Mei-Huei T. Lai , Sheila P. Little , George L. Long , Robert F. Santerre
发明人： Nils U. Bang , Robert J. Beckmann , S. Richard Jaskunas , Mei-Huei T. Lai , Sheila P. Little , George L. Long , Robert F. Santerre
IPC分类号： A61K35/12 , A61K31/00 , A61K35/64 , A61K35/74 , A61K38/16 , A61K38/43 , A61K38/46 , A61K38/48 , A61P7/00 , A61P7/02 , C07H21/04 , C07K14/00 , C07K14/47 , C07K14/745 , C12N1/20 , C12N1/21 , C12N5/00 , C12N5/10 , C12N9/64 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/57 , C12P21/00 , C12P21/02 , C12R1/07 , C12R1/19 , C12R1/91
CPC分类号： C12N9/6464 , C12Y304/21069 , Y10S435/832 , Y10S435/849 , Y10S435/886
摘要： The present invention comprises novel DNA compounds which encode human protein C activity. A variety of eukaryotic and prokaryotic recombinant DNA expression vectors have been constructed that comprise the novel protein C activity-encoding DNA and drive expression of protein C activity when transformed into an appropriate host cell. The novel expression vectors can be used to produce protein C derivatives, such as non-carboxylated, non-glycosylated, or non-hydroxylated protein C, and to produce protein C precursors, such as nascent or zymogen protein C, and to produce sub-fragments of protein C, such as active or inactive light and heavy chain. The recombinant-produced protein C activity is useful in the treatment and prevention of a variety of vascular disorders.
摘要翻译：本发明包括编码人蛋白C活性的新型DNA化合物。已经构建了多种真核和原核重组DNA表达载体，其包含新的蛋白质C活性编码DNA，并在转化到合适的宿主细胞中时驱动蛋白C活性的表达。新型表达载体可用于产生蛋白C衍生物，例如非羧基化，非糖基化或非羟基化蛋白质C，并产生蛋白质C前体，例如新生或酶原蛋白C，蛋白C片段，如活性或非活性轻链和重链。重组产生的蛋白质C活性可用于治疗和预防多种血管疾病。

2. 发明授权

US4775624A Vectors and compounds for expression of human protein C 失效
标题翻译：用于表达人蛋白C的载体和化合物
公开(公告)号：US4775624A
公开(公告)日：1988-10-04
申请号：US699967
申请日：1985-02-08
申请人： Nils U. Bang , Robert J. Beckmann , S. Richard Jaskunas , Mei-Huei T. Lai , Shelia P. Little , George L. Long , Robert F. Santerre
发明人： Nils U. Bang , Robert J. Beckmann , S. Richard Jaskunas , Mei-Huei T. Lai , Shelia P. Little , George L. Long , Robert F. Santerre
IPC分类号： A61K35/12 , A61K31/00 , A61K35/64 , A61K35/74 , A61K38/16 , A61K38/43 , A61K38/46 , A61K38/48 , A61P7/00 , A61P7/02 , C07H21/04 , C07K14/00 , C07K14/47 , C07K14/745 , C12N1/20 , C12N1/21 , C12N5/00 , C12N5/10 , C12N9/64 , C12N15/00 , C12N15/09 , C12N15/12 , C12N15/57 , C12P21/00 , C12P21/02 , C12R1/07 , C12R1/19 , C12R1/91 , C07H15/12
CPC分类号： C12N9/6464 , C12Y304/21069 , Y10S435/832 , Y10S435/849 , Y10S435/886
摘要： The present invention comprises novel DNA compounds which encode human protein C activity. A variety of eukaryotic and prokaryotic recombinant DNA expression vectors have been constructed that comprise the novel protein C activity-encoding DNA and drive expression of protein C activity when transformed into an appropriate host cell. The novel expression vectors can be used to produce protein C derivatives, such as non-carboxylated, non-glycosylated, or non-hydroxylated protein C, and to produce protein C precursors, such as nascent or zymogen protein C, and to produce sub-fragments of protein C, such as active or inactive light and heavy chain. The recombinant-produced protein C activity is useful in the treatment and prevention of a variety of vascular disorders.
摘要翻译：本发明包括编码人蛋白C活性的新型DNA化合物。已经构建了多种真核和原核重组DNA表达载体，其包含新的蛋白质C活性编码DNA，并在转化到合适的宿主细胞中时驱动蛋白C活性的表达。新型表达载体可用于产生蛋白C衍生物，例如非羧基化，非糖基化或非羟基化蛋白质C，并产生蛋白质C前体，例如新生或酶原蛋白C，蛋白C片段，如活性或非活性轻链和重链。重组产生的蛋白质C活性可用于治疗和预防多种血管疾病。

3. 发明授权

US5270040A Vectors and compounds for expression of human protein C 失效
标题翻译：用于表达人蛋白C的载体和化合物
公开(公告)号：US5270040A
公开(公告)日：1993-12-14
申请号：US907499
申请日：1992-07-01
申请人： Nils U. Bang , Robert J. Beckmann , S. R. Jaskunas , Mei-Huei T. Lai , Sheila P. Little , George L. Long , Robert F. Santerre
发明人： Nils U. Bang , Robert J. Beckmann , S. R. Jaskunas , Mei-Huei T. Lai , Sheila P. Little , George L. Long , Robert F. Santerre
IPC分类号： C12N9/64 , A61K37/547
CPC分类号： C12N9/6464 , C12Y304/21069
摘要： The present invention comprises novel DNA compounds which encode human protein C activity. A variety of eukaryotic and prokaryotic recombinant DNA expression vectors have been constructed that comprise the novel protein C activity-encoding DNA and drive expression of protein C activity when transformed into an appropriate host cell. The novel expression vectors can be used to produce protein C derivatives, such as non-carboxylated, non-glycosylated, or non-hydroxylated protein C, and to produce protein C precursors, such as nascent or zymogen protein C, and to produce subfragments of protein C, such as active or inactive light and heavy chain. The recombinant-produced protein C activity is useful in the treatment and prevention of a variety of vascular disorders.
摘要翻译：本发明包括编码人蛋白C活性的新型DNA化合物。已经构建了多种真核和原核重组DNA表达载体，其包含新的蛋白质C活性编码DNA，并在转化到合适的宿主细胞中时驱动蛋白C活性的表达。新的表达载体可用于产生蛋白质C衍生物，例如非羧化的，非糖基化的或非羟基化的蛋白质C，并产生蛋白质C前体，例如新生或酶原蛋白C，并产生亚克隆蛋白C，如活性或非活性轻链和重链。重组产生的蛋白质C活性可用于治疗和预防多种血管疾病。

4. 发明授权

US4727028A Recombinant DNA cloning vectors and the eukaryotic and prokaryotic transformants thereof 失效
标题翻译：重组DNA克隆载体及其真核和原核转化体
公开(公告)号：US4727028A
公开(公告)日：1988-02-23
申请号：US538051
申请日：1983-09-30
申请人： Robert F. Santerre , Ramachandra N. Rao
发明人： Robert F. Santerre , Ramachandra N. Rao
IPC分类号： C12N15/00 , C12N5/00 , C07H21/04 , C12N1/00 , C12N1/14 , C12N1/16 , C12N1/20 , C12N9/12 , C12P19/34 , C12P21/00
CPC分类号： C12N15/00
摘要： The present invention comprises novel recombinant DNA cloning and expression vectors which confer hygromycin B and/or G418 resistance to eukaryotic and prokaryotic host cells. The novel recombinant DNA vectors are derived from plasmid pKC203, a plasmid which can be isolated from E. coli JR225 (ATCC 31912). The hygromycin B and G418 resistance-conferring genes can be isolated on the 7.5 kb BglII restriction fragment or the 2.5 kb SalI-BglII restriction fragment of plasmid pKC203. The eukaryotic recombinant DNA vectors of the present invention are prepared by inserting such resistance-conferring restriction fragments into a vector, such as plasmid pSV5gpt, that comprises a eukaryotic promoter and the necessary functions for maintenance of the vector as an extrachromosomal element or as an integrated sequence in the host cell chromosomal DNA. Furthermore, the present invention comprises useful derivatives of plasmid pKC203 which, although comprising no eukaryotic elements, are useful recombinant vectors for E. coli and other prokaryotes and serve as starting material for the construction of eukaryotic vectors that confer hygromycin B and/or G418 resistance to eukaryotic host cells. One useful derivative of plasmid pKC203 is constructed by circularizing the .about.7.5 kb BglIII restriction fragment of plasmid pKC203 to form plasmid pSC701, which can be further digested with HaeII or SauIIIA1 to form smaller plasmids. The present invention also comprises the novel transformants of the aforementioned recombinant DNA vectors.
摘要翻译：本发明包括赋予真核细胞和原核宿主细胞的潮霉素B和/或G418抗性的新型重组DNA克隆和表达载体。新型重组DNA载体衍生自质粒pKC203，该质粒可从大肠杆菌JR225（ATCC 31912）中分离。可以在质粒pKC203的7.5kb BglII限制性片段或2.5kb SalI-BglII限制性片段上分离潮霉素B和G418抗性赋予基因。本发明的真核重组DNA载体是通过将这种产生抗性的限制性片段插入到包含真核启动子的载体（例如质粒pSV5gpt）中，以及维持作为染色体外元件的载体的必需功能宿主细胞染色体DNA序列。此外，本发明包括质粒pKC203的有用衍生物，其虽然不含真核生物元件，但是是用于大肠杆菌和其他原核生物的有用的重组载体，并且用作构建赋予潮霉素B和/或G418抗性的真核载体的起始材料到真核宿主细胞。通过使质粒pKC203的DIFFERENCE 7.5kb BglIII限制性片段环化形成质粒pSC701构建质粒pKC203的一个有用的衍生物，其可以用HaeII或SauIIIA1进一步消化以形成较小的质粒。本发明还包括上述重组DNA载体的新型转化体。

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