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    • 5. 发明申请
    • Branched-Chain Fatty Acids And Biological Production Thereof
    • 分支链脂肪酸及其生物生产
    • US20110151526A1
    • 2011-06-23
    • US12972822
    • 2010-12-20
    • Charles Winston SaundersJun XuPhillip Richard GreenDavid Blair CodyZubin Saresh Khambatta
    • Charles Winston SaundersJun XuPhillip Richard GreenDavid Blair CodyZubin Saresh Khambatta
    • C12P7/64C12N1/21
    • C12N9/1029C12N9/0008C12N9/88C12N15/70C12P7/6409C12Y102/04004C12Y203/0118C12Y203/01182C12Y403/01019
    • A method for producing anteiso fatty acid is provided. The method comprises culturing a cell comprising at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes at least one of the following reactions: conversion of pyruvate to citramalate; conversion of citramalate to citraconate; conversion of citraconate to β-methyl-D-malate; conversion of β-methyl-D-malate to 2-oxobutanoate; or conversion of threonine to 2-oxobutanoate, under conditions allowing expression of the polynucleotide(s) and production of anteiso fatty acid. Optionally the cell further comprises at least one exogenous or overexpressed polynucleotide comprising a nucleic acid sequence encoding a polypeptide that catalyzes conversion of 2-oxobutanoate to 2-aceto-2-hydroxy-butyrate, conversion of 2-aceto-2-hydroxy-butyrate to 2,3-dihydroxy-3-methylvalerate, and/or conversion of 2,3-dihydroxy-3-methylvalerate to α-keto-3-methylvalerate. A cell that produces anteiso fatty acid and a method of using the cell to produce anteiso fatty acid also are provided.
    • 提供了制备异头物脂肪酸的方法。 该方法包括培养包含至少一种外源或过表达多核苷酸的细胞,所述多核苷酸包含编码多肽的核酸序列,所述核酸序列催化至少一个以下反应:将丙酮酸转化成柠檬酸盐; 柠檬酸盐向柠康酸盐的转化; 柠檬酸酯转化为β-马来酸甲酯; 将β-马来酸甲酯转化为2-氧代丁酸酯; 或在允许表达多核苷酸和生产异构脂肪酸的条件下将苏氨酸转化为2-氧代丁酸酯。 任选地,细胞还包含至少一种外源或过表达的多核苷酸,其包含编码催化2-氧代丁酸酯转化为2-乙酰-2-羟基丁酸的多肽的核酸序列,将2-乙酰-2-羟基 - 丁酸酯转化为 2,3-二羟基-3-甲基戊酸酯,和/或2,3-二羟基-3-甲基戊酸酯转化为α-酮-3-甲基戊酸酯。 还提供了产生异头物脂肪酸的细胞和使用该细胞产生异头物脂肪酸的方法。