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1. 发明授权

US08241893B2 Method and device for separating molecular targets in a complex mixture 有权
标题翻译：在复杂混合物中分离分子靶的方法和装置
公开(公告)号：US08241893B2
公开(公告)日：2012-08-14
申请号：US11892672
申请日：2007-08-24
申请人： Nicolas Ugolin , Sylvie Chevillard , Alexadre Coutant , Jerome LeBeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Alexadre Coutant , Jerome LeBeau
IPC分类号： C12M1/36 , G01N15/06 , C07H21/04
CPC分类号： G01N33/5438 , B01J2219/00497 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/502761 , B01L3/502792 , B01L2300/0636 , B01L2300/0645 , B01L2300/0822 , B01L2300/0864 , B01L2300/0867 , C12Q1/6837 , C12Q2565/513 , C12Q2545/114
摘要： The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.
摘要翻译：本发明涉及一种分析复合混合物中所含的分子靶的方法，包括以下步骤：a）使待分析的分子靶标的混合物与不同类型的主要探针的阵列接触，由此每种形成阵列的主要探针可以在能够在分子靶标和主要探针之间进行特异性结合的条件下特异性地结合选自分子靶标的靶标类型; b）任选地消除不与分子靶特异性结合的主要探针; c）分离特异性结合在探针/靶复合体中的分子靶和主要探针，例如恢复代表要分析的分子靶标的指纹的主要探针阵列; 和d）定量分析步骤c中洗脱的主要探针。

2. 发明申请

US20130012400A1 METHOD AND DEVICE FOR SEPARATING MOLECULAR TARGETS IN A COMPLEX MIXTURE 审中-公开
标题翻译：用于在复合混合物中分离分子靶的方法和装置
公开(公告)号：US20130012400A1
公开(公告)日：2013-01-10
申请号：US13465636
申请日：2012-05-07
申请人： Nicolas Ugolin , Sylvie Chevillard , Alexadre Coutant , Jerome Lebeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Alexadre Coutant , Jerome Lebeau
IPC分类号： C40B20/04 , C40B30/04
CPC分类号： G01N33/5438 , B01J2219/00497 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/502761 , B01L3/502792 , B01L2300/0636 , B01L2300/0645 , B01L2300/0822 , B01L2300/0864 , B01L2300/0867 , C12Q1/6837 , C12Q2565/513 , C12Q2545/114
摘要： The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.
摘要翻译：本发明涉及一种分析复合混合物中所含的分子靶的方法，包括以下步骤：a）使待分析的分子靶标的混合物与不同类型的主要探针的阵列接触，由此每种形成阵列的主要探针可以在能够在分子靶标和主要探针之间进行特异性结合的条件下特异性地结合选自分子靶标的靶标类型; b）任选地消除不与分子靶特异性结合的主要探针; c）分离特异性结合在探针/靶复合体中的分子靶和主要探针，例如恢复代表要分析的分子靶标的指纹的主要探针阵列; 和d）定量分析步骤c中洗脱的主要探针。

3. 发明授权

US07687257B2 Device for separating and/or analyzing several molecular targets dissolved in a complex mixture 失效
标题翻译：用于分离和/或分析溶解在复杂混合物中的若干分子靶的装置
公开(公告)号：US07687257B2
公开(公告)日：2010-03-30
申请号：US11386781
申请日：2006-03-23
申请人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01L3/502761 , B01L2300/0819 , B01L2400/0406 , B01L2400/0415 , B01L2400/0478 , G01N1/405 , G01N27/44773 , G01N27/44782
摘要： The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.
摘要翻译：本发明涉及用于分离和/分析溶解在复合混合物中的几种分子靶的装置，其特征在于其包括a）微柱基质，其中每个微柱（2）包含固定的分子探针，用于保留通过特异性探针/靶连接物包含在复合物混合物中的特定分子靶，b）用于将引入本发明装置的复合物混合物循环到a），（c）中定义的基质的每个微柱的第一毛细管网（3）第二毛细管网络（4），用于在洗脱后使保留在微柱上的分子靶朝向传感器（5）循环，用于进行其回收和/或分析，以及d）如果需要，传感器（5），优选以质谱仪的形式，用于进行不同分子靶标的回收和/或分析。

4. 发明申请

US20060239864A1 Device for separating and/or analyzing several molecular targets dissolved in a complex mixture 失效
标题翻译：用于分离和/或分析溶解在复杂混合物中的若干分子靶的装置
公开(公告)号：US20060239864A1
公开(公告)日：2006-10-26
申请号：US11386781
申请日：2006-03-23
申请人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
IPC分类号： G01N1/00
CPC分类号： B01L3/502715 , B01L3/502761 , B01L2300/0819 , B01L2400/0406 , B01L2400/0415 , B01L2400/0478 , G01N1/405 , G01N27/44773 , G01N27/44782
摘要： The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.
摘要翻译：本发明涉及用于分离和/分析溶解在复合混合物中的几种分子靶的装置，其特征在于其包括a）微柱基质，其中每个微柱（2）包含固定的分子探针，用于保留通过特异性探针/靶连接物包含在复合物混合物中的特定分子靶，b）用于将引入本发明装置的复合物混合物循环到a），（c）中定义的基质的每个微柱的第一毛细管网（3）第二毛细管网络（4），用于在洗脱后使保留在微柱上的分子靶朝向传感器（5）循环，用于进行其回收和/或分析，以及d）如果需要，传感器（5），优选以质谱仪的形式，用于进行不同分子靶标的回收和/或分析。

5. 发明授权

US08211693B2 Device for separating and/or analyzing several molecular targets dissolved in a complex mixture 失效
标题翻译：用于分离和/或分析溶解在复杂混合物中的若干分子靶的装置
公开(公告)号：US08211693B2
公开(公告)日：2012-07-03
申请号：US12700680
申请日：2010-02-04
申请人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Catherine Ory , Jerome Lebeau
IPC分类号： C12M1/34
CPC分类号： B01L3/502715 , B01L3/502761 , B01L2300/0819 , B01L2400/0406 , B01L2400/0415 , B01L2400/0478 , G01N1/405 , G01N27/44773 , G01N27/44782
摘要： The invention relates to a device for separating and/analyzing several molecular targets dissolved in a complex mixture which is characterized in that it comprises a) a matrix of micro-columns, wherein each micro-column (2) comprises an immobilized molecular probe for retaining a specific molecular target contained in the complex mixture by specific probe/target linkage, b) a first capillary network (3) for circulating the complex mixture introduced into the inventive device towards each micro-column of the matrix defined in a), c) a second capillary network (4) for circulating, after elution, the molecular targets retained on the micro-columns towards a sensor (5) for carrying out the recovery and/or analysis thereof, and d) if necessary, a sensor (5), preferably in the form of a mass spectrometer, for carrying out the recovery and/or analysis of different molecular targets.
摘要翻译：本发明涉及用于分离和/分析溶解在复合混合物中的几种分子靶的装置，其特征在于其包括a）微柱基质，其中每个微柱（2）包含固定的分子探针，用于保留通过特异性探针/靶连接物包含在复合物混合物中的特定分子靶，b）用于将引入本发明装置的复合物混合物循环到a），c）中定义的基质的每个微柱的第一毛细管网（3）第二毛细管网络（4），用于在洗脱后使保留在微柱上的分子靶朝向传感器（5）循环，用于进行其回收和/或分析，以及d）如果需要，传感器（5），优选以质谱仪的形式，用于进行不同分子靶标的回收和/或分析。

6. 发明申请

US20080182758A1 Method and device for separating molecular targets in a complex mixture 有权
标题翻译：在复杂混合物中分离分子靶的方法和装置
公开(公告)号：US20080182758A1
公开(公告)日：2008-07-31
申请号：US11892672
申请日：2007-08-24
申请人： Nicolas Ugolin , Sylvie Chevillard , Alexandre Coutant , Jerome Lebeau
发明人： Nicolas Ugolin , Sylvie Chevillard , Alexandre Coutant , Jerome Lebeau
IPC分类号： C40B30/04 , C40B60/12 , C40B40/08 , C40B50/00
CPC分类号： G01N33/5438 , B01J2219/00497 , B01J2219/005 , B01J2219/00527 , B01J2219/00605 , B01J2219/00653 , B01J2219/00722 , B01J2219/00725 , B01J2219/00729 , B01L3/502761 , B01L3/502792 , B01L2300/0636 , B01L2300/0645 , B01L2300/0822 , B01L2300/0864 , B01L2300/0867 , C12Q1/6837 , C12Q2565/513 , C12Q2545/114
摘要： The invention relates to a method of analysing molecular targets contained in a complex mixture, comprising the following steps consisting in: a) bringing the mixture of molecular targets to be analysed into contact with an array of different types of primary probes, whereby each type of primary probe forming the array can bind specifically to a type of target selected from among the molecular targets, under conditions that enable specific binding between the molecular targets and the primary probes; b) optionally eliminating the primary probes that are not bound specifically to a molecular target; c) separating the molecular targets and the primary probes which are bound specifically in a probe/target complex, such as to recover the array of primary probes representing a fingerprint of the molecular targets to be analysed; and d) quantitatively analysing the primary probes eluted in step c.
摘要翻译：本发明涉及一种分析复合混合物中所含的分子靶的方法，包括以下步骤：a）使待分析的分子靶标的混合物与不同类型的主要探针的阵列接触，由此每种形成阵列的主要探针可以在能够在分子靶标和主要探针之间进行特异性结合的条件下特异性地结合选自分子靶标的靶标类型; b）任选地消除不与分子靶特异性结合的主要探针; c）分离特异性结合在探针/靶复合体中的分子靶和主要探针，例如恢复代表要分析的分子靶标的指纹的主要探针阵列; 和d）定量分析步骤c中洗脱的主要探针。

7. 发明申请

US20090054264A1 Method of Fabricating an Array of Capillaries on a Chip 审中-公开
标题翻译：在芯片上制造毛细管阵列的方法
公开(公告)号：US20090054264A1
公开(公告)日：2009-02-26
申请号：US12224423
申请日：2007-02-26
申请人： Nicolas Ugolin , Pascal Wodling , Julien Le Meur , Pierre-Yves Tro , Sylvie Chevillard
发明人： Nicolas Ugolin , Pascal Wodling , Julien Le Meur , Pierre-Yves Tro , Sylvie Chevillard
IPC分类号： C40B40/00 , C40B50/18 , C40B60/12
CPC分类号： B01L3/502707 , B01L2200/12 , B01L2300/0816 , B01L2300/0887
摘要： The invention relates to a method of fabricating an array of capillaries of a chip, the method comprising the steps consisting in depositing at least one layer of a meltable or polymerizable construction material on a support plate, focusing and moving a laser beam on and over said layer respectively to melt or polymerize the material so as to form the side walls of the capillaries, and then fastening a cover plate on the side walls of the capillaries. The invention also provides a chip including an array of capillaries in which chemical or biological molecules are fixed, and a chip including an array of chromatography and/or electrophoresis capillaries.
摘要翻译：本发明涉及一种制造芯片的毛细管阵列的方法，该方法包括以下步骤：在支撑板上沉积至少一层可熔化或可聚合的建筑材料，聚焦并移动所述的层以熔化或聚合材料以形成毛细管的侧壁，然后将盖板紧固在毛细管的侧壁上。本发明还提供一种芯片，其包括固定有化学或生物分子的毛细管阵列，以及包括色谱和/或电泳毛细管阵列的芯片。

8. 发明申请

US20100022400A1 METHOD FOR THE QUANTITATIVE MEASUREMENT OF BIOMOLECULAR TARGETS DEPOSITED ON A BIOCHIP, AND DEVICE FOR IMPLEMENTING IT 有权
标题翻译：生物分子量目标定量测定方法，以及生物分子实验装置
公开(公告)号：US20100022400A1
公开(公告)日：2010-01-28
申请号：US12441225
申请日：2007-09-14
申请人： Nicolas Ugolin , Denis Menut , Julien Le Meur , Pascal Wodling , Sylvie Chevillard
发明人： Nicolas Ugolin , Denis Menut , Julien Le Meur , Pascal Wodling , Sylvie Chevillard
IPC分类号： C40B30/00 , C40B60/12
CPC分类号： G01N21/718 , B33Y80/00
摘要： The present invention relates in particular to a method for the quantitative measurement of biomolecular targets that have been deposited on a biochip (1) of the type with a matrix of probes hybridized by the targets, the matrix comprising a multitude of measurement points (2) each comprising a plurality of probes, characterized in that it comprises the following steps: a) at least one laser beam (18) is focused onto each measurement point, in order to extract therefrom a hot confined plasma comprising a chemical element to be quantified that is present in the targets and optionally in the probes; b) the light emission lines from the plasma are detected and analysed for each measurement point, by measuring the respective intensities of these lines; and then c) the concentration in each measurement point of the element or of a group incorporating it within the targets is determined via a prior calibration of the lines establishing a correlation between the intensities of the lines specific to the element to be quantified and given concentrations of this element.
摘要翻译：本发明特别涉及一种用于定量测量生物分子靶标的方法，该方法已经沉积在具有由靶标杂交的探针基质的类型的生物芯片（1）上，该基质包含多个测量点（2）每个包括多个探针，其特征在于，其包括以下步骤：a）至少一个激光束（18）聚焦到每个测量点上，以便从其中提取包含要量化的化学元素的热限制等离子体，存在于目标物中，任选地存在于探针中; b）通过测量这些线的相应强度，对每个测量点检测和分析来自等离子体的发光线; 然后c）通过先前校准线确定要量化的元素和给定浓度的特异性线之间的相关性来确定元件或其中包含在靶中的组的每个测量点中的浓度的元素。

9. 发明授权

US07136517B2 Image analysis process for measuring the signal on biochips 失效
标题翻译：用于测量生物芯片上信号的图像分析过程
公开(公告)号：US07136517B2
公开(公告)日：2006-11-14
申请号：US10173672
申请日：2002-06-19
申请人： Nicolas Ugolin , Mory Doukoure , Olivier Alibert , Sylvie Chevillard
发明人： Nicolas Ugolin , Mory Doukoure , Olivier Alibert , Sylvie Chevillard
IPC分类号： G06K9/00
CPC分类号： G06T1/0007 , G06T7/0012 , G06T7/11 , G06T2207/10056 , G06T2207/10064 , G06T2207/20056 , G06T2207/30072
摘要： The invention relates to an image analysis process for measuring the signal on biochips organized into one or several blocks each comprising a large number of spots each composed of at least one probe, the said process being characterized in that it comprises the following steps: a deposit is made with a constant pitch, the spots appearing in the form of a periodic and regular signal; this periodicity property is used to identify the position of the blocks and the spots, this periodicity being searched for on projections of the image in the x and y directions.
摘要翻译：本发明涉及一种用于测量组织成一个或多个块的生物芯片上的信号的图像分析过程，每个块包括大量由至少一个探针组成的点，所述方法的特征在于其包括以下步骤：沉积物以恒定音调制成，斑点以周期性和规则信号的形式出现; 该周期特性用于识别块和斑点的位置，在x和y方向上的图像的投影上搜索该周期。

10. 发明授权

US08675192B2 Method and device for high speed quantitative measurement of biomolecular targets on or in biological analysis medium 失效
标题翻译：在生物分析培养基中或生物分析培养基中高速定量测定生物分子靶标的方法和装置
公开(公告)号：US08675192B2
公开(公告)日：2014-03-18
申请号：US12933350
申请日：2009-03-20
申请人： Nicolas Ugolin , Denis Menut , Julien Le Meur , Nadine Coulon , Sylvie Chevillard , Emilie Bosc , Jean-Marc Joseph Desaulniers
发明人： Nicolas Ugolin , Denis Menut , Julien Le Meur , Nadine Coulon , Sylvie Chevillard , Emilie Bosc , Jean-Marc Joseph Desaulniers
IPC分类号： G01J3/30
CPC分类号： G01N21/718 , G01N21/6452
摘要： The present invention relates to a device and method for the high speed quantitative measurement of biomolecular targets on the surface or in the body of a planar medium for biological analysis. The method, according to the invention, includes the following, steps: a) at least two laser beams (F″) are focused and overlaid on each measuring point of said medium by the simultaneous intersection of these beams to extract a contained hot plasma (P), including a measured chemical element present in the targets and another chemical element exogenous to the targets and present in a known quantity on this medium; b) luminous emission rays for each plasma, corresponding to the quantified element and exogenous element, are detected and analyzed for each measuring point while measuring the brightness of these rays; then c) the concentration in each measuring point of the quantified element is determined through prior calibration of the rays of the qualified element to determine a correlation between the brightness of the rays, specific to said element, and the concentrations of the latter in mixtures of the quantified element and exogenous element in known proportions.
摘要翻译：本发明涉及用于生物分析的平面介质的表面或体内的生物分子靶的高速定量测定的装置和方法。根据本发明的方法包括以下步骤：a）至少两个激光束（F“）通过这些光束的同时交叉来聚焦并重叠在所述介质的每个测量点上以提取所含的热等离子体（P），包括目标中存在的测量化学元素和另外一种对目标外源的化学元素，并以该已知量存在于该介质上; b）在测量这些光线的亮度的同时，对每个测量点检测并分析对应于量化元素和外源元素的每个等离子体的发光射线; 然后c）通过先前校准合格元素的射线来确定量化元件的每个测量点中的浓度，以确定特定于所述元素的射线的亮度与后者的亮度之间的相关性，已知比例的量化元素和外源元素。

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