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    • 4. 发明申请
    • METHOD AND SYSTEM FOR CHARACTERIZING A PIGMENTED BIOLOGICAL TISSUE
    • 用于表征生物生物组织的方法和系统
    • US20100184117A1
    • 2010-07-22
    • US12602530
    • 2008-05-27
    • Zoran CerovicNicolae MoiseGwendal LatoucheYves Goulas
    • Zoran CerovicNicolae MoiseGwendal LatoucheYves Goulas
    • C12Q1/02
    • G01N21/64G01N21/274G01N21/645G01N2021/6419G01N2021/6421G01N2021/6491G01N2201/0221G01N2201/062G01N2201/0627
    • Method for determining the content of a non-fluorescent chromophorous first compound, in a biological tissue including a fluorescent chromophorous second compound, the method includes at least one iteration of the following operations: emission, in the direction of said tissue, of a first reference optical radiation, and a second measurement optical radiation, each chosen so as to induce a fluorescence radiation of the second compound, each of the first and second radiations being partially absorbed by the first compound, measuring the fluorescence radiations induced by each of the first and second radiations, and determining, from the measurement, of the content of the first compound in the tissue, wherein the method includes at least one compensation of a measurement saturation due to too high an absorption of the measurement radiation by the first compound, the compensation comprising a choice, for the measurement radiation, of a wavelength corresponding to a lower absorption in the absorption spectrum of the first compound.
    • 用于确定包含荧光色度第二化合物的生物组织中的非荧光色原子化合物的含量的方法,所述方法包括以下操作的至少一次迭代:在所述组织的方向上发射第一参考 光辐射和第二测量光辐射,每个被选择为引起第二化合物的荧光辐射,第一和第二辐射中的每一个被第一化合物部分地吸收,测量由第一和第二化合物中的每一个引起的荧光辐射, 第二辐射,以及从测量确定组织中第一化合物的含量,其中所述方法包括由于第一化合物对测量辐射的吸收太高而对测量饱和度的至少一次补偿,补偿 包括对于测量辐射的对应于较低吸收的波长的选择 第一化合物的吸收光谱。