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    • 1. 发明申请
    • METHODS AND SYSTEMS FOR IDENTIFICATION OF DNA PATTERNS THROUGH SPECTRAL ANALYSIS
    • 通过光谱分析识别DNA图案的方法和系统
    • US20090129647A1
    • 2009-05-21
    • US12282435
    • 2007-03-07
    • Nevenka DimitrovaYee Him Cheung
    • Nevenka DimitrovaYee Him Cheung
    • G06K9/00
    • G06F19/22G06F19/24G06F19/26
    • Spectrogram extraction from DNA sequence has been known since 2001. A DNA spectrogram is generated by applying Fourier transform to convert a symbolic DNA sequence consisting of letters A, T, C, G into a visual representation that highlights periodicities of co-occurrence of DNA patterns. Given a DNA sequence or whole genomes, with this method it is easy to generate a large number of spectrogram images. However, the difficult part is to elucidate where are the repetitive patterns and to associate a biological and clinical meaning to them. The present disclosure provides systems and methods that facilitate the location and/or identification of repetitive DNA patterns, such as CpG islands, Alu repeats, tandem repeats and various types of satellite repeats. These repetitive elements can be found within a chromosome, within a genome or across genomes of various species. The disclosed systems and methods apply image processing operators to find prominent features in the vertical and horizontal direction of the DNA spectrograms. Systems and methods for fast, full scale analysis of the derived images using supervised machine learning methods are also disclosed. The disclosed systems and methods for detecting and/or classifying repetitive DNA patterns include: (a) comparative histogram method, (b) feature selection and classification using support vector machines and genetic algorithms, and (c) generation of spectrovideo from a plurality of spectral images.
    • 从2001年开始,DNA序列的谱图提取是已知的。通过应用傅立叶变换将由字母A,T,C,G组成的符号DNA序列转换成突出显示DNA图案共同出现周期的视觉表示,生成DNA谱图 。 给定一个DNA序列或整个基因组,用这种方法很容易产生大量的谱图。 然而,困难的部分是阐明重复模式在哪里,并将生物和临床意义与他们联系起来。 本公开提供了促进重复DNA模式(例如CpG岛,Alu重复序列,串联重复序列和各种类型的卫星重复)的定位和/或识别的系统和方法。 这些重复元件可以在染色体内,基因组内或各种物种的基因组中发现。 所公开的系统和方法应用图像处理算子以在DNA谱图的垂直和水平方向上发现显着特征。 还公开了使用监督机器学习方法对衍生图像进行快速,全面分析的系统和方法。 用于检测和/或分类重复DNA模式的所公开的系统和方法包括:(a)比较直方图方法,(b)使用支持向量机和遗传算法的特征选择和分类,以及(c)从多个光谱 图片。
    • 4. 发明授权
    • Methods and systems for identification of DNA patterns through spectral analysis
    • 通过光谱分析鉴定DNA模式的方法和系统
    • US08189892B2
    • 2012-05-29
    • US12282435
    • 2007-03-07
    • Nevenka DimirovaYee Him Cheung
    • Nevenka DimirovaYee Him Cheung
    • G06K9/00
    • G06F19/22G06F19/24G06F19/26
    • Systems and methods facilitate the location and/or identification of repetitive DNA patterns, such as CpG islands, Alu repeats, tandem repeats and various types of satellite repeats. These repetitive elements can be found within a chromosome, within a genome or across genomes of various species. The systems and methods apply image processing operators to find prominent features in the vertical and horizontal direction of the DNA spectrograms. The systems and methods for detecting and/or classifying repetitive DNA patterns include: (a) a comparative histogram method, (b) feature selection and classification using support vector machines and genetic algorithms, and (c) generation of a spectrovideo from a plurality of spectral images.
    • 系统和方法有助于重复DNA模式的定位和/或识别,例如CpG岛,Alu重复序列,串联重复和各种类型的卫星重复。 这些重复元件可以在染色体内,基因组内或各种物种的基因组中发现。 系统和方法应用图像处理算子来查找DNA谱图的垂直和水平方向的突出特征。 用于检测和/或分类重复DNA模式的系统和方法包括:(a)比较直方图方法,(b)使用支持向量机和遗传算法的特征选择和分类,以及(c)从多个 光谱图像。