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    • 5. 发明授权
    • Detection of mutational frequency and related methods
    • 检测突变频率及相关方法
    • US07255993B1
    • 2007-08-14
    • US10704283
    • 2003-11-06
    • Neal S. YoungSachiko Kajigaya
    • Neal S. YoungSachiko Kajigaya
    • C12Q1/68C12P19/34C07H21/04
    • C12Q1/6883C12Q2600/156
    • Methods are provided herein for measuring the mutational frequency of a DNA molecule in cells, for example stem cells or hematopoietic cells such as CD34+ cells or granulocytes. The method includes sequencing corresponding regions of mtDNA from a set of hematopoietic cells, or a set of clonal populations of hematopoietic cells, and comparing the sequence of the corresponding regions of mtDNA from the cells, or clonal populations of cells. The method also includes the comparison of mtDNA sequences with genomic DNA sequences. Also provided are methods for screening for an agent that has a mutagenic effect on a cell. The method includes contacting, or treating, clonal populations of cells with an agent and comparing the sequence of the mtDNA obtained from the treated clonal populations of cells, with the sequence of the corresponding region of mtDNA obtained from a control clonal populations of cells.
    • 本文提供了用于测量细胞中DNA分子的突变频率的方法,例如干细胞或造血细胞例如CD34 +细胞或粒细胞。 该方法包括从一组造血细胞或一组造血细胞的克隆群体中对相应的mtDNA区域进行测序,并比较来自细胞或细胞的克隆群体的mtDNA的相应区域的序列。 该方法还包括mtDNA序列与基因组DNA序列的比较。 还提供了筛选对细胞具有诱变作用的试剂的方法。 该方法包括使细胞与克隆的细胞群接触或处理,并将从经处理的细胞群体获得的mtDNA的序列与从对照克隆细胞群获得的mtDNA的相应区域的序列进行比较。
    • 9. 发明申请
    • DETECTION OF MUTATIONAL FREQUENCY AND RELATED METHODS
    • 检测频率和相关方法
    • US20070259370A1
    • 2007-11-08
    • US11770456
    • 2007-06-28
    • Neal YoungSachiko Kajigaya
    • Neal YoungSachiko Kajigaya
    • C12Q1/68
    • C12Q1/6883C12Q2600/156
    • Methods are provided herein for measuring the mutational frequency of a DNA molecule in cells, for example stem cells or hematopoietic cells such as CD34+ cells or granulocytes. The method includes sequencing corresponding regions of mtDNA from a set of hematopoietic cells, or a set of clonal populations of hematopoietic cells, and comparing the sequence of the corresponding regions of mtDNA from the cells, or clonal populations of cells. The method also includes the comparison of mtDNA sequences with genomic DNA sequences. Also provided are methods for screening for an agent that has a mutagenic effect on a cell. The method includes contacting, or treating, clonal populations of cells with an agent and comparing the sequence of the mtDNA obtained from the treated clonal populations of cells, with the sequence of the corresponding region of mtDNA obtained from a control clonal populations of cells.
    • 本文提供了用于测量细胞中DNA分子的突变频率的方法,例如干细胞或造血细胞例如CD34 +细胞或粒细胞。 该方法包括从一组造血细胞或一组造血细胞的克隆群体中对相应的mtDNA区域进行测序,并比较来自细胞或细胞的克隆群体的mtDNA的相应区域的序列。 该方法还包括mtDNA序列与基因组DNA序列的比较。 还提供了筛选对细胞具有诱变作用的试剂的方法。 该方法包括使细胞与克隆的细胞群接触或处理,并将从经处理的细胞群体获得的mtDNA的序列与从对照克隆细胞群获得的mtDNA的相应区域的序列进行比较。