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1. 发明授权

US09371519B2 Complex type sugar chain hydrolase 有权
标题翻译：复合型糖链水解酶
公开(公告)号：US09371519B2
公开(公告)日：2016-06-21
申请号：US14349569
申请日：2012-10-03
申请人： National Institute of Advanced Industrial Science and Technology
发明人： Yasunori Chiba , Satoshi Murakami , Hisashi Narimatsu
IPC分类号： C12N9/24 , C12N9/42 , C12P21/00
CPC分类号： C12N9/2434 , C12N9/2402 , C12P21/005 , C12Y302/01052 , C12Y302/01096 , Y02P20/52
摘要： The present invention provides a novel endo-β-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-β-N-acetylglucosaminidase (Endo-Om) gene originated from a methylotrophic yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including complex type sugar chains, in glycoproteins and the modification of the sugar chains. Also provided are an endo-β-N-acetylglucosaminidase (Endo-Cp), an endo-β-N-acetylglucosaminidase (Endo-Pa) and an endo-β-N-acetylglucosamimidase (Endo Zr) which are produced from Candida parapolymorpha DL-1 ATCC26012 strain, Pichia anomala ATCC36904 strain and Zygosaccharomyces rouxii ATCC2623 strain, respectively, on the basis of an Endo-Om gene sequence, and each of which has a similar level of complex type sugar chain cleavage activity and a similar level of complex type sugar chain transfer activity to those of Endo-Om.
摘要翻译：本发明提供了一种利用克隆衍生于甲基营养酵母Ogataea minuta IFO10746菌株的内切-G-乙酰氨基葡萄糖苷酶（Endo-Om）基因产生的转化体的新型内切-N-乙酰氨基葡糖苷酶（Endo-Om）。根据本发明的内消旋体具有比已知Endo-M高13倍的比活性，和比已知Endo-M高55倍的Vmax值，并且可用于分析结构的糖链，包括复合型糖链，糖蛋白和糖链的修饰。还提供了内源性N-乙酰氨基葡萄糖苷酶（Endo-Cp），内含量 - 乙酰氨基葡糖苷酶（Endo-Pa）和内含量 - 乙酰葡糖苷酶（Endo Zr），其由基于Endo-Om基因序列，分别具有类似水平的复合型糖链切割活性和类似水平的Paradolymorpha DL-1 ATCC26012菌株，异
毕赤酵母ATCC36904菌株和接合酵母属（Lygosaccharomyces rouxii）ATCC2623菌株的复合型糖链转移活性与Endo-Om的活性。

2. 发明申请

US20140315246A1 COMPOSITE SUGAR CHAIN HYDROLASE 有权
标题翻译：复合糖链水解酶
公开(公告)号：US20140315246A1
公开(公告)日：2014-10-23
申请号：US14349569
申请日：2012-10-03
申请人： National Institute of Advanced Industrial Science and Technology
发明人： Yasunori Chiba , Satoshi Murakami , Hisashi Narimatsu
IPC分类号： C12N9/42 , C12P21/00
CPC分类号： C12N9/2434 , C12N9/2402 , C12P21/005 , C12Y302/01052 , C12Y302/01096 , Y02P20/52
摘要： The present invention provides a novel endo-β-N-acetylglucosaminidase (Endo-Om) using a transformant produced by cloning an endo-β-N-acetylglucosaminidase (Endo-Om) gene originated from a methylotrophic yeast Ogataea minuta IFO10746 strain. The Endo-Om according to the present invention has a specific activity 13-fold higher than that of known Endo-M and a Vmax value 55-fold higher than that of the known Endo-M, and is useful for the analysis of the structures of sugar chains, including complex type sugar chains, in glycoproteins and the modification of the sugar chains. Also provided are an endo-β-N-acetylglucosaminidase (Endo-Cp), an endo-β-N-acetylglucosaminidase (Endo-Pa) and an endo-β-N-acetylglucosamimidase (Endo Zr) which are produced from Candida parapolymorpha DL-1 ATCC26012 strain, Pichia anomala ATCC36904 strain and Zygosaccharomyces rouxii ATCC2623 strain, respectively, on the basis of an Endo-Om gene sequence, and each of which has a similar level of complex type sugar chain cleavage activity and a similar level of complex type sugar chain transfer activity to those of Endo-Om.
摘要翻译：本发明提供了一种利用克隆衍生于甲基营养酵母Ogataea minuta IFO10746菌株的内切-G-乙酰氨基葡萄糖苷酶（Endo-Om）基因产生的转化体的新型内切-N-乙酰氨基葡糖苷酶（Endo-Om）。根据本发明的内消旋体具有比已知Endo-M高13倍的比活性，和比已知Endo-M高55倍的Vmax值，并且可用于分析结构的糖链，包括复合型糖链，糖蛋白和糖链的修饰。还提供了内源性N-乙酰氨基葡萄糖苷酶（Endo-Cp），内含量 - 乙酰氨基葡糖苷酶（Endo-Pa）和内含量 - 乙酰葡糖苷酶（Endo Zr），其由基于Endo-Om基因序列，分别具有类似水平的复合型糖链切割活性和类似水平的Paradolymorpha DL-1 ATCC26012菌株，异
毕赤酵母ATCC36904菌株和接合酵母属（Lygosaccharomyces rouxii）ATCC2623菌株的复合型糖链转移活性与Endo-Om的活性。

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