专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明授权

US10156576B2 Method for enrichment and separation of spinal fluid glycoprotein, method for searching for marker for central nervous system diseases which utilizes the aforementioned method, and marker for central nervous system diseases 有权
公开(公告)号：US10156576B2
公开(公告)日：2018-12-18
申请号：US15071419
申请日：2016-03-16
申请人： National Institute of Advanced Industrial Science and Technology
发明人： Hisashi Narimatsu , Jun Hirabayashi , Atsushi Kuno , Hideki Matsuzaki , Yuzuru Ikehara , Hiromi Ito , Yasuhiro Hashimoto , Keiro Shirotani , Satoshi Futakawa , Hajime Arai , Masakazu Miyajima , Kazuo Fujihara
IPC分类号： G01N33/68 , H01J49/00
摘要： The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.

2. 发明授权

US09914908B2 Method of introducing a toxin or dye into mammalian pluripotent cells using rBC2LCN 有权
公开(公告)号：US09914908B2
公开(公告)日：2018-03-13
申请号：US14767880
申请日：2014-02-13
申请人： National Institute of Advanced Industrial Science and Technology
发明人： Hiroaki Tateno , Yuzuru Ito , Yasuko Onuma , Jun Hirabayashi , Makoto Asashima
IPC分类号： C12N5/00 , C07K14/195 , C12N9/10
CPC分类号： C12N5/0081 , C07K14/195 , C07K2319/33 , C07K2319/55 , C12N9/1077 , C12Y204/02036
摘要： An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.

3. 发明授权

US09279809B2 Cell differentiation assay method, cell isolation method, method for producing induced pluripotent stem cells, and method for producing differentiated cells 有权
标题翻译：细胞分化试验方法，细胞分离方法，诱导多能干细胞的制备方法和分化细胞的制备方法
公开(公告)号：US09279809B2
公开(公告)日：2016-03-08
申请号：US14381116
申请日：2013-02-27
申请人： National Institute of Advanced Industrial Science and Technology , Wako Pure Chemical Industries, Ltd.
发明人： Hiroaki Tateno , Jun Hirabayashi , Makoto Asashima , Yuzuru Ito , Yasuko Onuma , Masaki Warashina , Masakazu Fukuda
IPC分类号： G01N33/569
CPC分类号： G01N33/56966
摘要： Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc;” and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.”
摘要翻译：提供了通过仅选择性染色未分化状态的干细胞来精确评估干细胞的分化状态的方法，以及仅将未分化状态的干细胞仅阳性分离的方法。具体提供了一种用于确定细胞分化的方法，包括使测试细胞与包含以下蛋白质（A）或（B）的探针接触的步骤和检测探针与测试细胞结合的步骤的步骤。用于确定细胞分化的方法能够通过使用与未分化干细胞特异性反应并检测与测试细胞结合的存在的探针来检测测试细胞中是否存在未分化干细胞。（A）包含SEQ ID NO：1所示的氨基酸序列并识别“Fucα1-2Gal”和“3GlcNAc”和/或“Fucα1-2Gal”和“1-3GalNAc”的糖链结构的蛋白质和（B）包含与SEQ ID NO：1所示的氨基酸序列具有80％以上相似性的氨基酸序列的蛋白质，并且识别“Fucα1-2Gal”和“3GlcNAc”的糖链结构和/或“Fucα1-2Gal” -3GalNAc“。

4. 发明申请

US20150111218A1 CELL DIFFERENTIATION ASSAY METHOD, CELL ISOLATION METHOD, METHOD FOR PRODUCING INDUCED PLURIPOTENT STEM CELLS, AND METHOD FOR PRODUCING DIFFERENTIATED CELLS 有权
标题翻译：细胞分化测定方法，细胞分离方法，产生诱导型细胞干细胞的方法和生产差异细胞的方法
公开(公告)号：US20150111218A1
公开(公告)日：2015-04-23
申请号：US14381116
申请日：2013-02-27
申请人： National Institute of Advanced Industrial Science and Technology , Wako Pure Chemical Industries, Ltd.
发明人： Hiroaki Tateno , Jun Hirabayashi , Makoto Asashima , Yuzuru Ito , Yasuko Onuma , Masaki Warashina , Masakazu Fukuda
IPC分类号： G01N33/569
CPC分类号： G01N33/56966
摘要： Provided are a method for accurately evaluating the differentiation status of stem cells by selectively staining only stem cells in an undifferentiated state, and a method for positively isolating only stem cells in an undifferentiated state. Specifically provided is a method for determining differentiation of a cell comprising a step of contacting a test cell with a probe comprising protein (A) or (B) below and a step of detecting the presence of binding of the probe to the test cell. The method for determining differentiation of a cell is capable of detecting the presence or absence of an undifferentiated stem cell in test cells by using a probe that specifically reacts with undifferentiated stem cells and detecting the presence of bonding to the test cell. (A) A protein comprising an amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc;” and (B) a protein comprising an amino acid sequence showing 80% or more similarity to the amino acid sequence shown in SEQ ID NO: 1 and recognizing a sugar chain structure of “Fucα1-2Galβ1-3GlcNAc” and/or “Fucα1-2Galβ1-3GalNAc.”
摘要翻译：提供了通过仅选择性染色未分化状态的干细胞来精确评估干细胞的分化状态的方法，以及仅将未分化状态的干细胞仅阳性分离的方法。具体提供了一种用于确定细胞分化的方法，包括使测试细胞与包含以下蛋白质（A）或（B）的探针接触的步骤和检测探针与测试细胞结合的步骤的步骤。用于确定细胞分化的方法能够通过使用与未分化干细胞特异性反应并检测与测试细胞结合的存在的探针来检测测试细胞中是否存在未分化干细胞。（A）包含SEQ ID NO：1所示的氨基酸序列并识别“Fucα1-2Gal”和“3GlcNAc”和/或“Fucα1-2Gal”和“1-3GalNAc”的糖链结构的蛋白质和（B）包含与SEQ ID NO：1所示的氨基酸序列具有80％以上相似性的氨基酸序列的蛋白质，并且识别“Fucα1-2Gal”和“3GlcNAc”的糖链结构和/或“Fucα1-2Gal” -3GalNAc“。

5. 发明申请

US20150204870A1 UNDIFFERENTIATED CELL DETECTION METHOD AND COMPLEX CARBOHYDRATE DETECTION METHOD 有权
标题翻译：不稳定细胞检测方法和复合碳水化合物检测方法
公开(公告)号：US20150204870A1
公开(公告)日：2015-07-23
申请号：US14355413
申请日：2012-10-31
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY , WAKO PURE CHEMICAL INDUSTRIES, LTD.
发明人： Hiroaki Tateno , Jun Hirabayashi , Yuzuru Ito , Yasuko Onuma , Makoto Asashima , Atsushi Kuno , Masaki Warashina , Masakazu Fukuda
IPC分类号： G01N33/569 , C07K14/195
CPC分类号： G01N33/56966 , C07K14/195 , G01N2333/4724 , G01N2400/00
摘要： An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fucα1-2Galβ1-3GlcNAc” or “Fucα1-2Galβ1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.
摘要翻译：本发明的目的是提供一种使用干细胞的培养物上清液评估细胞分化状态的方法。提供由糖链结构“Fucα1-2Gal＆bgr; 1-3GlcNAc”或“Fucα1-2Gal＆bgr; 1-3GalNAc”组成的“未分化糖链标记”，其能够使用茎的培养物上清液敏感地测定干细胞的未分化状态细胞。还发现BC2LCN凝集素或其能够敏感识别“未分化糖链标记”的优化“能够用培养上清液测定细胞未分化状态的”未分化糖链标志物的探针“的修饰产物。

6. 发明授权

US09500650B2 Undifferentiated cell detection method and complex carbohydrate detection method 有权
标题翻译：未分化细胞检测方法和复杂碳水化合物检测方法
公开(公告)号：US09500650B2
公开(公告)日：2016-11-22
申请号：US14355413
申请日：2012-10-31
申请人： National Institute of Advanced Industrial Science and Technology , Wako Pure Chemical Industries, Ltd.
发明人： Hiroaki Tateno , Jun Hirabayashi , Yuzuru Ito , Yasuko Onuma , Makoto Asashima , Atsushi Kuno , Masaki Warashina , Masakazu Fukuda
IPC分类号： G01N33/569 , C07K14/195
CPC分类号： G01N33/56966 , C07K14/195 , G01N2333/4724 , G01N2400/00
摘要： An object of the present invention is to provide a method for evaluating a differentiation status of cells using a culture supernatant of stem cells. Provided is “undifferentiation sugar chain marker” composed of the sugar chain structure “Fucα1-2Galβ1-3GlcNAc” or “Fucα1-2Galβ1-3GalNAc” and capable of sensitively determining the undifferentiated state of stem cells using a culture supernatant of stem cells. Also found is BC2LCN lectin or a modified product thereof capable of sensitively recognizing the “undifferentiation sugar chain marker” as excellent “probe for detecting the undifferentiation sugar chain marker” capable of determining the undifferentiation status of cells using a culture supernatant.
摘要翻译：本发明的目的是提供一种使用干细胞的培养物上清液评估细胞分化状态的方法。提供由糖链结构“Fucα1-2Galβ1-3GlcNAc”或“Fucα1-2Galβ1-3GalNAc”组成的能够使用干细胞的培养上清液敏感地测定干细胞的未分化状态的“未分化糖链标记”。还发现BC2LCN凝集素或其能够敏感识别“未分化糖链标记”的优化“能够用培养上清液测定细胞未分化状态的”未分化糖链标志物的探针“的修饰产物。

7. 发明申请

US20160187356A1 METHOD FOR ENRICHMENT AND SEPARATION OF SPINAL FLUID GLYCOPROTEIN, METHOD FOR SEARCHING FOR MARKER FOR CENTRAL NERVOUS SYSTEM DISEASES WHICH UTILIZES THE AFOREMENTIONED METHOD, AND MARKER FOR CENTRAL NERVOUS SYSTEM DISEASES 审中-公开
标题翻译：用于增强和分离脊髓液糖蛋白的方法，用于使用利用方法的中央神经系统疾病的标记的方法和用于中央神经系统疾病的标记的方法
公开(公告)号：US20160187356A1
公开(公告)日：2016-06-30
申请号：US15071419
申请日：2016-03-16
申请人： National Institute of Advanced Industrial Science and Technology
发明人： Hisashi Narimatsu , Jun Hirabayashi , Atsushi Kuno , Hideki Matsuzaki , Yuzuru Ikehara , Hiromi Ito , Yasuhiro Hashimoto , Keiro Shirotani , Satoshi Futakawa , Hajime Arai , Masakazu Miyajima , Kazuo Fujihara
IPC分类号： G01N33/68 , H01J49/00
CPC分类号： G01N33/6896 , G01N33/6842 , G01N2333/47 , G01N2333/4728 , G01N2400/40 , G01N2800/285 , H01J49/0036
摘要： The purpose of the present invention is to develop: a method for selectively separating a glycoprotein derived from the central nervous system from a body fluid or a central nervous system cell; and a method for searching for an index marker for central nervous system diseases, which utilizes the aforementioned method. A protein derived from the central nervous system, which occurs in a trace amount in a body fluid or a central nervous system cell, can be selectively enriched by a two-stage separation procedure comprising removing a glycoprotein having sialic acid at a non-reducing terminal thereof from the body fluid or the central nervous system cell and then separating a glycoprotein having N-acetylglucosamine at a non-reducing terminal thereof.
摘要翻译：本发明的目的是开发：从体液或中枢神经系统细胞中选择性分离衍生自中枢神经系统的糖蛋白的方法; 以及利用上述方法搜索中枢神经系统疾病指标的方法。可以通过两步分离方法选择性地富集来自中枢神经系统的蛋白质，其在体液或中枢神经系统细胞中发生微量的蛋白质，其包括在非还原末端除去具有唾液酸的糖蛋白从体液或中枢神经系统细胞中分离，然后在其非还原末端分离具有N-乙酰葡糖胺的糖蛋白。

8. 发明申请

US20150376568A1 UNDIFFERENTIATED CELL ELIMINATION METHOD 有权
标题翻译：不完全细胞消除方法
公开(公告)号：US20150376568A1
公开(公告)日：2015-12-31
申请号：US14767880
申请日：2014-02-13
申请人： NATIONAL INSTITUTE OF ADVANCED INDUSTRIAL SCIENCE AND TECHNOLOGY
发明人： Hiroaki Tateno , Yuzuru Ito , Yasuko Onuma , Jun Hirabayashi , Makoto Asashima
IPC分类号： C12N5/00 , C12N9/10 , C07K14/195
CPC分类号： C12N5/0081 , C07K14/195 , C07K2319/33 , C07K2319/55 , C12N9/1077 , C12Y204/02036
摘要： An object of the present invention is to provide a method for introducing a target substance into undifferentiated cells, and a carrier therefor, whereby the target substance can be specifically introduced into the undifferentiated cells by contacting the undifferentiated cells with an rBC2LCN-target substance fusion product in which rBC2LCN lectin is fused to the target substance. Particularly, an rBC2LCN-toxin fusion product in which rBC2LCN lectin is fused to a toxin functioning in cells or its domain having the ability to kill cells functions as an agent for eliminating undifferentiated stem cells and can be administered into a medium after inducing the differentiation of the stem cells to reliably kill only the stem cells in an undifferentiated state.
摘要翻译：本发明的目的是提供一种将未靶标物质引入未分化细胞的方法及其载体，通过使未分化细胞与rBC2LCN靶物质聚合物接触，可以将目标物质特异性地引入未分化细胞中其中rBC2LCN凝集素与靶物质融合。特别地，其中rBC2LCN凝集素与在细胞中起作用的毒素或其具有杀死细胞功能的结构域的毒素融合的rBC2LCN-毒素融合产物用作消除未分化干细胞的药剂，并且可以在诱导分化后的细胞中施用于培养基干细胞可靠地只杀死未分化状态的干细胞。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式