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1. 发明申请

US20070048838A1 Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method 失效
公开(公告)号：US20070048838A1
公开(公告)日：2007-03-01
申请号：US11589842
申请日：2006-10-31
申请人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
发明人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
IPC分类号： C07H21/04 , C12P21/06 , C12N9/48 , C12N15/74 , C12N1/21
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.

2. 发明授权

US07288388B2 Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method 失效
标题翻译：肽形成酶基因，肽形成酶和肽生产方法
公开(公告)号：US07288388B2
公开(公告)日：2007-10-30
申请号：US10763249
申请日：2004-01-26
申请人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
发明人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
IPC分类号： C12P21/06 , C12N9/48 , C12N5/00 , C12N1/20 , C07H21/04
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
摘要翻译：本发明提供了通过工业上有利且简单的途径使用廉价可获得的原料生产二肽的方法。使用从培养物分离的微生物细胞，使用从L-氨基酸酯和L-氨基酸产生二肽的微生物培养物，由L-氨基酸酯和L-氨基酸产生二肽，或处理微生物细菌产物。

3. 发明申请

US20050037453A1 Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method 失效
标题翻译：肽形成酶基因，肽形成酶和肽生产方法
公开(公告)号：US20050037453A1
公开(公告)日：2005-02-17
申请号：US10763249
申请日：2004-01-26
申请人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
发明人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
IPC分类号： C07K5/062 , C07K5/06 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/48 , C12N9/52 , C12N9/80 , C12N15/57 , C12N15/63 , C12P21/00 , C12P21/02 , C12P21/06 , C07H21/04 , C12N9/64
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
摘要翻译：本发明提供了通过工业上有利且简单的途径使用廉价可获得的原料生产二肽的方法。使用从培养物分离的微生物细胞，使用从L-氨基酸酯和L-氨基酸产生二肽的微生物培养物，由L-氨基酸酯和L-氨基酸产生二肽，或处理微生物细菌产物。

4. 发明授权

US07618796B2 Peptide-forming enzyme gene, peptide-forming enzyme, and peptide producing method 失效
标题翻译：肽形成酶基因，肽形成酶和肽生产方法
公开(公告)号：US07618796B2
公开(公告)日：2009-11-17
申请号：US11589842
申请日：2006-10-31
申请人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
发明人： Naoto Tonouchi , Sonoko Suzuki , Kenzo Yokozeki , Hiroyuki Nozaki , Masakazu Sugiyama
IPC分类号： C12P21/02
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： The present invention provides a method for producing dipeptide using inexpensively acquirable starting materials by an industrially advantageous and simple pathway. Dipeptide is produced from L-amino acid ester and L-amino acid using a culture of microbes having the ability to produce a dipeptide from an L-amino acid ester and an L-amino acid, using microbial cells isolated from the culture, or a treated microbial cell product of the microbe.
摘要翻译：本发明提供了通过工业上有利且简单的途径使用廉价可获得的原料生产二肽的方法。使用从培养物分离的微生物细胞，使用从L-氨基酸酯和L-氨基酸产生二肽的微生物培养物，由L-氨基酸酯和L-氨基酸产生二肽，或处理微生物细菌产物。

5. 发明申请

US20070042459A1 Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 审中-公开
标题翻译：二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法
公开(公告)号：US20070042459A1
公开(公告)日：2007-02-22
申请号：US11249429
申请日：2005-10-14
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
IPC分类号： C12P21/06 , C12N9/48
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译：提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物。该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

6. 发明授权

US07037673B2 Dipeptide production method, L-amino acid amide hydrolase used therein, and production method of L-amino acid amide hydrolase 失效
标题翻译：二肽生产方法，其中使用的L-氨基酸酰胺水解酶，以及L-氨基酸酰胺水解酶的制备方法
公开(公告)号：US07037673B2
公开(公告)日：2006-05-02
申请号：US10481087
申请日：2002-07-26
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
IPC分类号： C12P21/00 , C12N9/58
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译：提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物。该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

7. 发明申请

US20050054067A1 Process for producing dipeptide,l-amino acid amide hydrolase to be used therein and process for producing l-amino acid amide hydrolase 失效
标题翻译：用于生产二肽，用于其中的1-氨基酰胺水解酶的方法和用于生产1-氨基酸酰胺水解酶的方法
公开(公告)号：US20050054067A1
公开(公告)日：2005-03-10
申请号：US10481087
申请日：2002-07-26
申请人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Ikuo Kira , Sonoko Suzuki , Kenzo Yokozeki
IPC分类号： C07K5/062 , C07K5/06 , C12N1/19 , C12N1/21 , C12N9/00 , C12N9/48 , C12N9/52 , C12N9/80 , C12N15/57 , C12N15/63 , C12P21/00 , C12P21/02 , C12P21/06 , C12P1/02
CPC分类号： C12P21/02 , C07K5/06026 , C12N9/48
摘要： A process for industrially advantageously producing a dipeptide via a convenient pathway starting with less expensive and easily available materials is provided. A dipeptide is produced from an L-amino acid amide and an L-amino acid by using a culture of a microbe capable of synthesizing the dipeptide from the L-amino acid amide and the L-amino acid, microbial cells separated from the culture or a treated microbial cell product from the microbe. An L-amino acid amide hydrolase is obtained from a microbe belonging to the genus erwinia, genus Rhodococcus, genus Chryseobacterium, genus Micrococcus, genus Cryptococcus, genus Trichosporion, genus Rhodosporidium, genus Sporobolomyces, genus Tremela, genus Torulaspora, genus Sterigmatomyces or genus Rhodotorula. The hydrolase catalyzes a reaction that produces a dipeptide from an L-amino acid amide and an L-amino acid.
摘要翻译：提供了一种工业上有利地通过便宜的途径产生二肽的方法，从较便宜且容易获得的材料开始。通过使用能够从L-氨基酸酰胺和L-氨基酸合成二肽的微生物培养物从L-氨基酸酰胺和L-氨基酸产生二肽，从培养物分离的微生物细胞或来自微生物的经处理的微生物细胞产物。 L-氨基酸酰胺水解酶是从属于欧文氏菌属，红球菌属，巧克氏杆菌属，微球菌属，隐球菌属，三孢球菌属，红球菌属，孢子属，金龟子属，紫花a属，紫锥体属或红酵母属的微生物。该水解酶催化从L-氨基酸酰胺和L-氨基酸产生二肽的反应。

8. 发明授权

US07745182B2 Method for producing β-hydroxy amino acid and enzyme used therefor 有权
标题翻译：用于生产β-羟基氨基酸和酶的方法
公开(公告)号：US07745182B2
公开(公告)日：2010-06-29
申请号：US12345922
申请日：2008-12-30
申请人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C12P13/04 , C12N9/00 , C12N9/10
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.
摘要翻译：提供了一种生产β-羟基氨基酸及其光学活性异构体的方法。 β-羟基氨基酸通过使预定的D-α-氨基酸和5,10-亚甲基四氢叶酸在衍生自属于副球菌属，氨基杆菌属或Ensifer属的微生物的酶存在下反应来制备。

9. 发明授权

US07556947B2 Method for producing β-hydroxy amino acid and enzyme used therefor 有权
公开(公告)号：US07556947B2
公开(公告)日：2009-07-07
申请号：US12345887
申请日：2008-12-30
申请人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C12P13/04 , C12N9/00 , C12N9/10 , C12N1/20 , C12N15/74 , C07H21/04
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.

10. 发明申请

US20060263861A1 METHOD FOR PRODUCING BETA-HYDROXY AMINO ACID AND ENZYME USED THEREFOR 有权
公开(公告)号：US20060263861A1
公开(公告)日：2006-11-23
申请号：US11419522
申请日：2006-05-22
申请人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
发明人： Hiroyuki Nozaki , Shinji Kuroda , Kunihiko Watanabe , Kenzo Yokozeki
IPC分类号： C12P13/04 , C07H21/04 , C12P21/06 , C12N9/10 , C12N1/21 , C12N15/74
CPC分类号： C12P13/04 , C12N9/1014
摘要： A method for producing β-hydroxy amino acid and its optically-active isomer is provided. The β-hydroxy amino acid is produced by reacting a predetermined D-α-amino acid and 5,10-methylene tetrahydrofolic acid in the presence of an enzyme derived from a microorganism belonging to the genera Paracoccus, Aminobacter, or Ensifer.

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