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1. 发明授权

US07432102B2 Transformants producing substance pf1022 derivatives, methods for producing the same, and novel biosynthesis genes 失效
标题翻译：产生物质pf1022衍生物的转化体，其制备方法和新的生物合成基因
公开(公告)号：US07432102B2
公开(公告)日：2008-10-07
申请号：US10472587
申请日：2002-03-22
申请人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
发明人： Koji Yanai , Naomi Sumida , Manabu Watanabe , Tatsuki Moriya , Takeshi Murakami
IPC分类号： C12N1/00 , C12P1/00 , C12P17/00 , C12P15/00 , C12P13/00 , C12P7/00 , C12P7/24 , C12P1/02
CPC分类号： C12P17/14 , C12N15/52
摘要： An objective of the present invention is to provide a method for producing substance PF1022 derivatives, in particular PF1022-220 and PF1022-260, by a direct fermentation method, and a transformant to be used for this method. According to the present invention, there is provided a transformant producing substance FF1022 derivatives, which can be obtained by introducing a genes involved in a biosynthetic pathway from chorismic acid to p-aminophenylpyruvic acid, including a papA gene encoding 4-amino-4-deoxychorismate synthase. which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 2; a papB gene encoding 4-amino-4-deoxyclaismate mutase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 4; and a papC gene encoding 4-amino-4-deoxyprepbenate dehydrogenase, which gene comprises the DNA sequence encoding the amino acid sequence of SEQ ID NO: 6, into a phenylalanine auxotrophic host induced from an organism that produces a substance PF1022. According to the present invention, there is also provided a process of producing substance PF1022 derivative, comprising steps of culturing the above-mentioned transformant and collecting the substance PF1022 derivatives.
摘要翻译：本发明的目的是提供通过直接发酵方法生产物质PF1022衍生物，特别是PF1022-220和PF1022-260的方法，以及用于该方法的转化体。根据本发明，提供了转化体生产物质FF1022衍生物，其可以通过将参与生物合成途径的基因从氯磺酸引入对氨基苯基丙酮酸而获得，包括编码4-氨基-4-脱氧胸苷酸的papA基因合酶。该基因包含编码SEQ ID NO：2的氨基酸序列的DNA序列; 编码4-氨基-4-脱氧胆酸酯变位酶的papB基因，该基因包含编码SEQ ID NO：4的氨基酸序列的DNA序列; 和编码4-氨基-4-脱氧降磷酸脱氢酶的papC基因，该基因包含编码SEQ ID NO：6的氨基酸序列的DNA序列，由产生PF1022的生物体诱导的苯丙氨酸营养缺陷型宿主中。根据本发明，还提供了生产物质PF1022衍生物的方法，包括培养上述转化体并收集物质PF1022衍生物的步骤。

2. 发明授权

US08383392B2 Transformant and method for production of non-natural antibiotic 有权
标题翻译：用于生产非天然抗生素的转化体和方法
公开(公告)号：US08383392B2
公开(公告)日：2013-02-26
申请号：US12678101
申请日：2008-09-12
申请人： Naomi Sumida , Manabu Watanabe , Koji Yanai
发明人： Naomi Sumida , Manabu Watanabe , Koji Yanai
IPC分类号： C12N1/15 , C12P29/00
CPC分类号： C12P19/56 , C12N9/0006 , C12P17/06 , C12P19/44
摘要： Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.
摘要翻译：公开了通过将参与L-赖氨替康的生物合成的酮还原酶基因导入本来能够产生柔红霉素的放线杆菌中制备的转化体。还公开了使用转化体有效生产非天然柔红霉素衍生物的方法。转化体能够有效地产生非天然柔红霉素衍生物如表柔比星。

3. 发明申请

US20100255543A1 METHOD FOR PRODUCTION OF NON-NATURAL ANTIBIOTIC 有权
标题翻译：生产非天然抗生素的方法
公开(公告)号：US20100255543A1
公开(公告)日：2010-10-07
申请号：US12678101
申请日：2008-09-12
申请人： Naomi Sumida , Manabu Watanabe , Koji Yanai
发明人： Naomi Sumida , Manabu Watanabe , Koji Yanai
IPC分类号： C12P19/56 , C12N1/21
CPC分类号： C12P19/56 , C12N9/0006 , C12P17/06 , C12P19/44
摘要： Disclosed is a transformant prepared by introducing a ketoreductase gene involved in the biosynthesis of L-epivancosamine into an actinobacterium originally capable of producing daunorubicin. Also disclosed is a process of efficiently producing a non-natural daunorubicin derivative using the transformant. The transformant is capable of efficiently producing a non-natural daunorubicin derivative such as epidaunorubicin.
摘要翻译：公开了通过将参与L-赖氨替康的生物合成的酮还原酶基因导入本来能够产生柔红霉素的放线杆菌中制备的转化体。还公开了使用转化体有效生产非天然柔红霉素衍生物的方法。转化体能够有效地产生非天然柔红霉素衍生物如表柔比星。

4. 发明授权

US07553640B2 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US07553640B2
公开(公告)日：2009-06-30
申请号：US12010055
申请日：2008-01-18
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C07H21/04 , C12N9/24 , C12Q1/68
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

5. 发明授权

US07144718B2 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US07144718B2
公开(公告)日：2006-12-05
申请号：US11320412
申请日：2005-12-29
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12N9/24 , C12N15/00 , C12P21/06
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

6. 发明申请

US20080187972A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US20080187972A1
公开(公告)日：2008-08-07
申请号：US12010055
申请日：2008-01-18
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P7/02 , C12N9/00
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

7. 发明申请

US20060275862A1 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US20060275862A1
公开(公告)日：2006-12-07
申请号：US11504646
申请日：2006-08-16
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12P33/00 , C12P21/06 , C07H21/04 , C12N9/24 , C12N15/74 , C12N1/16
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

8. 发明授权

US07022508B2 Saponin-digesting enzymes, genes thereof and soyasapogenol B mass production system 失效
标题翻译：皂苷消化酶，其基因和大豆皂甙B大量生产系统
公开(公告)号：US07022508B2
公开(公告)日：2006-04-04
申请号：US10479787
申请日：2002-06-06
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12Q1/68 , C07H21/04 , C12N9/24 , C12P33/00 , C12R1/885
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.
摘要翻译：本发明提供具有皂苷分解活性的蛋白质，更具体地说，可以分解具有大豆皂甙元酚B作为糖苷配基的糖苷以制备大豆皂甙元B的蛋白质，编码这种蛋白质的多核苷酸以及大规模生产大豆皂甙元B的方法的蛋白质使用相同根据本发明的蛋白质涉及（a），（b）或（c），即（a）包含选自SEQ ID NO：2所示的氨基酸序列的氨基酸序列的蛋白质，4和6; （b）与包含（a）中描述的序列的氨基酸序列的蛋白质具有至少50％同源性且具有皂苷分解活性的蛋白质; 或（c）包含（a）中描述的具有一个或多个氨基酸残基被缺失，取代，插入或添加并具有皂角苷分解活性的修饰的氨基酸序列的蛋白质。

9. 发明授权

US06277596B1 Regulatory sequence of cellulase cbh1 genes originating in trichoderma viride and system for mass-producing proteins or peptides therewith 有权
标题翻译：来源于绿叶病毒的纤维素酶cbh1基因的调控序列及其大量生产蛋白质或肽的系统
公开(公告)号：US06277596B1
公开(公告)日：2001-08-21
申请号：US09254733
申请日：1999-05-07
申请人： Manabu Watanabe , Tatsuki Moriya , Kaoru Aoyagi , Naomi Sumida , Takeshi Murakami
发明人： Manabu Watanabe , Tatsuki Moriya , Kaoru Aoyagi , Naomi Sumida , Takeshi Murakami
IPC分类号： C12P2106
CPC分类号： C12Y302/01091 , C12N9/2437 , C12N9/2482 , C12N15/80 , C12Y302/01004 , C12Y302/01008
摘要： A high-yield production system for proteins and peptides has been established, and especially a high-yield production system for cellulase in Trichoderma viride and similar filamentous fungi. The cellulase cbh1 gene regulator sequence derived from Trichoderma viride gives high expression of target proteins. The regulator sequence can therefore be used for high-yield expression of target proteins, especially cellulase. In particular, 15 g/L of an endoglucanase derived from Humicola insolens was successfully produced with this regulator sequence.
摘要翻译：已经建立了高产量的蛋白质和肽的生产系统，特别是在绿色木霉和类似丝状真菌中的纤维素酶的高产量生产系统。衍生自绿色木霉的纤维素酶cbh1基因调控序列提供了靶蛋白的高表达。因此，调节序列可用于靶蛋白，特别是纤维素酶的高产量表达。特别地，用该调节剂序列成功地产生了15g / L源自Humicola insolens的内切葡聚糖酶。

10. 发明授权

US07790425B2 Saponin-decomposing enzyme, gene thereof and large-scale production system for producing soyasapogenol B 失效
公开(公告)号：US07790425B2
公开(公告)日：2010-09-07
申请号：US12591586
申请日：2009-11-24
申请人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
发明人： Manabu Watanabe , Naoki Mido , Takayoshi Tamura , Naomi Sumida , Takashi Yaguchi
IPC分类号： C12N9/24 , C12N15/00 , C12P21/06
CPC分类号： C12N9/2402 , C12N15/8243 , C12P33/00
摘要： The present invention provides a protein having saponin-decomposing activity, more specifically a protein which can decompose a glycoside having soyasapogenol B as an aglycone to produce soyasapogenol B, a polynucleotide encoding such a protein, and a method of producing soyasapogenol B on a large scale using the same. A protein according to the present invention are concerned with (a), (b) or (c), namely (a) a protein comprising an amino acid sequence selected from the group consisting of the amino acid sequences shown in SEQ ID NOs: 2, 4, and 6; (b) a protein that has at least 50% homology to the protein comprising the amino acid sequence of the sequence described in (a) and having saponin-decomposing activity; or (c) a protein comprising a modified amino acid sequence of the sequence described in (a) that has one or more amino acid residues deleted, substituted, inserted, or added and having saponin-decomposing activity.

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