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1. 发明申请

US20150024513A1 NOVEL METHOD FOR DETECTING ANTIGEN, AND APPARATUS USING SAME 审中-公开
标题翻译：用于检测抗原的新方法，以及使用其的装置
公开(公告)号：US20150024513A1
公开(公告)日：2015-01-22
申请号：US14379664
申请日：2013-02-20
申请人： NANOENTEK, INC.
发明人： Chan II Chung , Chang Seop Lee , Jeoung Ku Hwang , Jong Sik Jeong
IPC分类号： G01N33/543
CPC分类号： G01N33/54306 , G01N33/53 , G01N33/543
摘要： The present invention provides a method for detecting an antigen in an analysis sample, the method including: (a) contacting an analysis sample with a detection antibody with which a marker generating a detectable signal is combined and which specifically binds to the antigen; (b) contacting a capture antibody with the resultant product of step (a), the capture antibody specifically binding to an antigen to be detected; (c) contacting the detection antibody, with which the marker generating a detectable signal is combined, with a reference substance which is bound to a surface of a solid substrate and which includes an epitope to which the detection antibody specifically binds; (d) measuring signals generated from the markers of the resultant product of step (b) and the resultant product of step (c); and (e) analyzing the measured signals to determine the presence or absence and amount of the antigen in the analysis sample. The method for detecting an antigen of the present invention can control the flow and the reaction time of an analysis sample, thereby improving sensitivity and minimizing the influences by the concentration of the analysis sample or the temperature of the detection reaction, and thus improving stability, reliability, and reproducibility of data, when compared with the conventional method for detecting an antigen. Accordingly, the method and apparatus for detecting an antigen of the present invention can be easily operated without specialized skills, thereby instantly obtaining the presence or absence and amount of detection antigen in the analysis sample through on-site diagnosis.
摘要翻译：本发明提供了一种检测分析样品中的抗原的方法，所述方法包括：（a）使分析样品与产生可检测信号的标记物组合并与抗原特异性结合的检测抗体接触; （b）将捕捉抗体与步骤（a）的所得产物接触，所述捕获抗体特异性结合待检测的抗原; （c）将产生可检测信号的标记与其结合的检测抗体与结合到固体底物表面并包括检测抗体特异性结合的表位的参考物质接触; （d）测量从步骤（b）的所得产物的标记产生的信号和步骤（c）的所得产物; 和（e）分析测量的信号以确定分析样品中抗原的存在或不存在和量。检测本发明的抗原的方法可以控制分析样品的流动和反应时间，从而提高灵敏度并使分析样品的浓度或检测反应的温度的影响最小化，从而提高稳定性，与传统的抗原检测方法相比，数据的可靠性和可重复性。因此，本发明的抗原检测方法和装置可以在没有专门技术的情况下容易地进行操作，从而通过现场诊断立即获得分析样品中检测抗原的存在或不存在和量。

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