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    • 8. 发明授权
    • Method for the detection of lateral flow assay and strip and laser-induced epifluorescence and compact scanner therefor
    • 检测横向流量测定和条带和激光诱导的落射荧光的方法和紧凑型扫描仪
    • US08011228B2
    • 2011-09-06
    • US10585467
    • 2005-01-05
    • Kie-Bong NahmEui-Yeol ChoiJae-Hoon Kim
    • Kie-Bong NahmEui-Yeol ChoiJae-Hoon Kim
    • G01N13/00
    • G01N33/558G01N21/6428G01N21/645G01N21/8483
    • Disclosed is a lateral flow quantitative assay method capable of quantitatively determining the concentration and analyzing the spatial distribution of a disease marker by employing the principle of the laser-induced fluorescence detection technique, which is based on detecting emitted fluorescence when laser light is focused to the disease marker deposited onto a lateral flow quantitative assay chip. The present invention discloses a strip, a laser-induced epifluorescence detection device and a small scanner for the assay method. The present assay method is advantageous in terms of allowing quantitative point-of-care diagnostics in hospitals, being capable of specifically detecting a disease marker by optimizing a lateral flow assay biochip for diagnosis of a specific disease, allowing more accurate quantitative analysis of analytes, and being capable of simultaneously analyzing several cancer markers, reducing the hook effect and expanding the detection range and accurately measuring concentration of analytes.
    • 公开了一种侧流定量测定方法,其能够通过采用激光诱导荧光检测技术的原理定量测定浓度并分析疾病标记物的空间分布,该方法基于当激光聚焦于 疾病标记物沉积在侧流定量测定芯片上。 本发明公开了一种用于测定方法的条带,激光诱导的落射荧光检测装置和小型扫描仪。 本测定方法在医院中允许定量点护理诊断方面是有利的,能够通过优化用于诊断特定疾病的侧流测定生物芯片来特异性检测疾病标记,从而允许对分析物进行更准确的定量分析, 并能够同时分析几种癌症标志物,减少钩效应,扩大检测范围,并准确测量分析物的浓度。