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1. 发明授权

US6156548A Immobilization of enzymes with a fluidized bed for use in an organic medium 有权
标题翻译：用流化床固定酶用于有机介质
公开(公告)号：US6156548A
公开(公告)日：2000-12-05
申请号：US210192
申请日：1998-12-11
申请人： Morten Wurtz Christensen , Ole Kirk , Christian Pedersen
发明人： Morten Wurtz Christensen , Ole Kirk , Christian Pedersen
IPC分类号： C12N11/00 , C12N11/08 , C12N11/14 , C12P7/64
CPC分类号： C12N11/08 , C12N11/00 , C12N11/14
摘要： An immobilized enzyme preparation for use in an organic medium essentially devoid of free water is prepared using a fluidized bed. An enzyme-containing liquid medium is contacted with a particulate porous carrier which preferably has a particle size of 200-1000 .mu.m and a surface area of 20-1000 m.sup.2 /g, and volatile components of the liquid medium are removed to fix or adsorb the enzyme on the carrier. The carrier may have a hydrophilic surface and an amount of liquid medium is used to prevent agglomeration of the carrier. The enzyme can be adsorbed on a carrier having a hydrophobic surface, and the addition of a hygroscopic substance suppresses agglomeration of the carrier by absorbing excess liquid. The hygroscopic substance may be removed during the removal of volatile components. Contacting of the enzyme-containing liquid and carrier is in a fluidized bed where immobilization and removing volatile components are conducted simultaneously, or contacting is in mixer followed by removing volatile components in a fluidized bed. The enzyme-containing liquid may be atomized onto the carrier in the fluidized bed or in the mixer. Enzymes immobilized include lipase, and the immobilized lipase is used in a trans-esterification reaction.
摘要翻译：使用流化床制备用于基本上不含游离水的有机介质中的固定化酶制剂。将含酶液体介质与优选粒径为200-1000μm，表面积为20-1000m 2 / g的颗粒状多孔载体接触，除去液体介质的挥发性成分以固定或吸附载体上的酶。载体可以具有亲水表面，并且使用一定量的液体介质来防止载体的附聚。酶可以吸附在具有疏水性表面的载体上，并且吸湿性物质的添加通过吸收多余的液体来抑制载体的聚集。在去除挥发性组分期间可以除去吸湿性物质。含酶液体和载体的接触在流化床中，其中同时进行固定和除去挥发性组分，或者在混合器中接触，然后在流化床中除去挥发性组分。含酶液体可以在流化床或混合器中雾化到载体上。固定的酶包括脂肪酶，并且固定的脂肪酶用于酯交换反应。

2. 发明授权

US09303256B2 Immobilization of enzymes 有权
标题翻译：固定酶
公开(公告)号：US09303256B2
公开(公告)日：2016-04-05
申请号：US11541615
申请日：2006-10-02
申请人： Isabelle Mazeaud , Poul Borge Rosenius Poulsen , Morten Wurtz Christensen , Jesper Brask
发明人： Isabelle Mazeaud , Poul Borge Rosenius Poulsen , Morten Wurtz Christensen , Jesper Brask
IPC分类号： C12N11/04 , C12N11/14
CPC分类号： C12N11/14
摘要： The present invention relates to the immobilization of enzymes by adsorbing enzymes, a polyfunctional amine and a cross-linking agent onto a particulate porous carrier in a mixer apparatus or in a fluid bed apparatus.
摘要翻译：本发明涉及通过在混合器装置或流化床装置中将酶，多官能胺和交联剂吸附到颗粒状多孔载体上来固定酶。

3. 发明申请

US20110201066A1 PRODUCTION OF FATTY ACID ALKYL ESTERS BY USE OF TWO LIPOLYTIC ENZYMES 审中-公开
标题翻译：通过使用两种脂肪酶生产脂肪酸酯
公开(公告)号：US20110201066A1
公开(公告)日：2011-08-18
申请号：US13096448
申请日：2011-04-28
申请人： Masnobu Abo , Morten Wurtz Christensen , Zhengyu Hu
发明人： Masnobu Abo , Morten Wurtz Christensen , Zhengyu Hu
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , Y02E50/13
摘要： A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride and alcohol is contacted with a first lipolytic enzyme having a relatively higher activity on free fatty acids than on triglyceride and a second lipolytic enzyme having a relatively higher activity on triglyceride than on free fatty acids.
摘要翻译：一种生产脂肪酸烷基酯的方法，其中包含甘油三酯和醇的溶液与对游离脂肪酸具有比在甘油三酯上具有相对较高活性的第一种脂肪分解酶和对甘油三酯具有相对较高活性的第二种脂肪分解酶脂肪酸。

4. 发明申请

US20100047884A1 Method for Producing Biodiesel 失效
标题翻译：生物柴油生产方法
公开(公告)号：US20100047884A1
公开(公告)日：2010-02-25
申请号：US12531742
申请日：2008-04-09
申请人： Wim De Greyt , Marc Kellens , Hans Christian Holm , Morten Wurtz Christensen , Per Munk Nielsen
发明人： Wim De Greyt , Marc Kellens , Hans Christian Holm , Morten Wurtz Christensen , Per Munk Nielsen
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , C10G2300/1011 , C10G2300/4081 , C10L1/026 , Y02E50/13 , Y02P30/20
摘要： The invention relates to the utilisation of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C1-C3 alkyl alcohols.
摘要翻译：本发明涉及利用在生产脂肪酸和C 1 -C 3烷基醇的无溶剂方法中有效的微生物酶来生产生物柴油中具有大量游离脂肪酸含量的脂肪物质。

5. 发明授权

US09593352B2 Production of fatty acid alkyl esters by use of two lipolytic enzymes 有权
标题翻译：通过使用两种脂肪分解酶生产脂肪酸烷基酯
公开(公告)号：US09593352B2
公开(公告)日：2017-03-14
申请号：US13096448
申请日：2011-04-28
申请人： Masnobu Abo , Morten Wurtz Christensen , Zhengyu Hu
发明人： Masnobu Abo , Morten Wurtz Christensen , Zhengyu Hu
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , Y02E50/13
摘要： A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride and alcohol is contacted with a first lipolytic enzyme having a relatively higher activity on free fatty acids than on triglyceride and a second lipolytic enzyme having a relatively higher activity on triglyceride than on free fatty acids.
摘要翻译：一种生产脂肪酸烷基酯的方法，其中包含甘油三酯和醇的溶液与对游离脂肪酸具有比在甘油三酯上具有相对较高活性的第一种脂肪分解酶和对甘油三酯具有相对较高活性的第二种脂肪分解酶脂肪酸。

6. 发明申请

US20080199924A1 Production of Degummed Fatty Acid Alkyl Esters 有权
标题翻译：脱脂脂肪酸烷基酯的生产
公开(公告)号：US20080199924A1
公开(公告)日：2008-08-21
申请号：US11916052
申请日：2006-06-13
申请人： Hans Christian Holm , Per Munk Nielson , Morten Wurtz Christensen
发明人： Hans Christian Holm , Per Munk Nielson , Morten Wurtz Christensen
IPC分类号： C12P7/62
CPC分类号： C12P7/649 , Y02E50/13
摘要： The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.
摘要翻译：本发明涉及脂肪酸烷基酯如脂肪酸甲酯（FAME）和具有低水平杂质如脂肪酸的脂肪酸乙酯的方法。通过将两个工艺步骤组合成一个单一工艺步骤来简化本发明的方法，因此经济上便宜。该方法包括将水，醇，甘油三酯和/或游离脂肪酸与脂肪分解酶和磷脂酶混合。随后，将含有甘油，残余酶和大部分水解磷脂的水相从非水相中分离出来，从而降低非水相中磷脂的含量。

7. 发明申请

US20080113419A1 Production of Fatty Acid Alkyl Esters by Use of Two Lipolytic Enzymes 审中-公开
标题翻译：通过使用两种脂肪分解酶生产脂肪酸烷基酯
公开(公告)号：US20080113419A1
公开(公告)日：2008-05-15
申请号：US11813121
申请日：2006-01-10
申请人： Masanobu Abo , Morten Wurtz Christensen
发明人： Masanobu Abo , Morten Wurtz Christensen
IPC分类号： C12P7/64
CPC分类号： C12P7/649 , Y02E50/13
摘要： A method for producing fatty acid alkyl esters, wherein a solution comprising triglyceride and alcohol is contacted with a first lipolytic enzyme having a relatively higher activity on free fatty acids than on triglyceride and a second lipolytic enzyme having a relatively higher activity on triglyceride than on free fatty acids.
摘要翻译：一种生产脂肪酸烷基酯的方法，其中包含甘油三酯和醇的溶液与对游离脂肪酸具有比在甘油三酯上具有相对较高活性的第一种脂肪分解酶和对甘油三酯具有相对较高活性的第二种脂肪分解酶脂肪酸。

8. 发明授权

US08178326B2 Producing esters of fatty acid and C1-C3 alkyl alcohols 失效
标题翻译：生产脂肪酸和C 1 -C 3烷基醇的酯
公开(公告)号：US08178326B2
公开(公告)日：2012-05-15
申请号：US12531742
申请日：2008-04-09
申请人： Wim De Greyt , Marc Kellens , Hans Christian Holm , Morten Wurtz Christensen , Per Munk Nielsen
发明人： Wim De Greyt , Marc Kellens , Hans Christian Holm , Morten Wurtz Christensen , Per Munk Nielsen
IPC分类号： C12P7/62
CPC分类号： C12P7/649 , C10G2300/1011 , C10G2300/4081 , C10L1/026 , Y02E50/13 , Y02P30/20
摘要： The invention relates to the utilization of fatty materials with substantial free fatty acid content in the production of biodiesel by the use of microbial enzymes that are effective in a solvent-free process for the production of esters of fatty acids and C1-C3 alkyl alcohols.
摘要翻译：本发明涉及利用在生产脂肪酸和C 1 -C 3烷基醇的无溶剂方法中有效的微生物酶来生产生物柴油中具有大量游离脂肪酸含量的脂肪物质。

9. 发明申请

US20110257123A1 ARYL/ALKYL VINYL SULFONE HYALURONIC ACID DERIVATIVES 失效
标题翻译： ARYL / ALKYL VINYL SULFONE HYALURONIC ACID DERIVATIVES
公开(公告)号：US20110257123A1
公开(公告)日：2011-10-20
申请号：US12161717
申请日：2007-03-02
申请人： Corinne Eenschooten , Morten Wurtz Christensen
发明人： Corinne Eenschooten , Morten Wurtz Christensen
IPC分类号： A61K31/728 , A61P17/14 , A61P17/02 , C08B37/08 , A61P19/02
CPC分类号： A61K8/735 , A61K31/728 , A61K47/36 , A61K2800/52 , A61Q7/00 , A61Q19/00 , A61Q19/08 , C08B37/0072 , C08L5/08
摘要： A hyaluronic acid derivative, and methods of producing and using said derivative, the derivative comprising n repeating units and having the general structural formula (I), wherein, in at least one repeating unit, one or more of R1, R2, R3, R4 comprises an etherbound aryl/alkyl sulfone having the general structural fomula (II), wherein R comprises an alkyl- or aryl-group, and otherwise R1, R2, R3, R4 are hydroxyl groups, OH.
摘要翻译：透明质酸衍生物，以及所述衍生物的制造和使用方法，所述衍生物包含n个重复单元并具有通式结构式（I），其中在至少一个重复单元中，R 1，R 2，R 3，R 4中的一个或多个包括具有通式结构式（II）的醚键芳基/烷基砜，其中R包括烷基或芳基，并且否则R 1，R 2，R 3，R 4为羟基。

10. 发明授权

US08012724B2 Production of degummed fatty acid alkyl esters using both lipase and phospholipase in a reaction mixture 有权
标题翻译：在反应混合物中使用脂肪酶和磷脂酶制造脱胶脂肪酸烷基酯
公开(公告)号：US08012724B2
公开(公告)日：2011-09-06
申请号：US11916052
申请日：2006-06-13
申请人： Hans Christian Holm , Per Munk Nielsen , Morten Wurtz Christensen
发明人： Hans Christian Holm , Per Munk Nielsen , Morten Wurtz Christensen
IPC分类号： C12P7/62
CPC分类号： C12P7/649 , Y02E50/13
摘要： The present invention relates to a method for producing fatty acid alkyl esters, such as fatty acid methyl esters (FAME) and fatty acid ethyl esters with a low level of impurities such as phospholipids. The method of the invention is simplified by combining two process steps into one single process step and is therefore economically cheaper. The method includes mixing water, alcohol, triglyceride and/or free fatty acids a lipolytic enzyme and a phospholipase. Subsequently the aqueous phase, which contains glycerine, residual enzyme and most of the hydrolyzed phospholipids, is separated from the non-aqueous phase, whereby the content of phospholipids in the non-aqueous phase is reduced.
摘要翻译：本发明涉及脂肪酸烷基酯如脂肪酸甲酯（FAME）和具有低水平杂质如脂肪酸的脂肪酸乙酯的方法。通过将两个工艺步骤组合成一个单一工艺步骤来简化本发明的方法，因此在经济上更便宜。该方法包括将水，醇，甘油三酯和/或游离脂肪酸与脂肪分解酶和磷脂酶混合。随后，将含有甘油，残余酶和大部分水解磷脂的水相从非水相中分离出来，从而降低非水相中磷脂的含量。

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