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1. 发明申请

US20140051847A1 METHOD FOR ISOLATING DNA 审中-公开
标题翻译：分离DNA的方法
公开(公告)号：US20140051847A1
公开(公告)日：2014-02-20
申请号：US13639496
申请日：2009-09-11
申请人： Mohd Ali Hassan , Meisam Tabatabaei , Mohd Rafein Zakaria , Abdul Raha Rahim , Ander-Denis G. Wright , Yoshihito Shirai , Norhani Abdullah , Mehdi Shamsara , Kenji Sakai
发明人： Mohd Ali Hassan , Meisam Tabatabaei , Mohd Rafein Zakaria , Abdul Raha Rahim , Ander-Denis G. Wright , Yoshihito Shirai , Norhani Abdullah , Mehdi Shamsara , Kenji Sakai
IPC分类号： C12N15/10
CPC分类号： C12N15/1003 , C12N15/1017
摘要： The present invention provides a method for the isolation of nucleic acid from microbial cells in an environmental sample. The method includes preparing a suspension of the environmental sample, lysing the suspended sample with a buffered solution, adding sodium dodecylsulfate solution to the lysed suspended sample, carrying out solvent extraction and separation to obtain an aqueous phase, reacting the aqueous phase with solvents to generate an insoluble precipitate containing nucleic acid, and isolating the nucleic acid therefrom, thereby releasing high molecular weight nucleic acid pellets from the cells.
摘要翻译：本发明提供了一种从环境样品中的微生物细胞中分离核酸的方法。该方法包括制备环境样品的悬浮液，用缓冲溶液裂解悬浮样品，向裂解的悬浮样品中加入十二烷基硫酸钠溶液，进行溶剂萃取和分离以获得水相，使水相与溶剂反应以产生含有核酸的不溶性沉淀物，从其中分离核酸，从而从细胞中释放出高分子量的核酸颗粒。

2. 发明申请

US20110160427A1 METHOD OF EXTRACTING AND PURIFYING POLYHYDROXYALKANOATE BIOPLASTIC 审中-公开
标题翻译：提取和纯化聚羟基烷基酯生物活性的方法
公开(公告)号：US20110160427A1
公开(公告)日：2011-06-30
申请号：US12678631
申请日：2009-07-06
申请人： Mohd Ali Hassan , Phooi Tee Voon , Lai Yee Phang , Yoshihito Shirai
发明人： Mohd Ali Hassan , Phooi Tee Voon , Lai Yee Phang , Yoshihito Shirai
IPC分类号： C08G63/06
CPC分类号： C08G63/90 , C08G63/06 , C12P7/625
摘要： Polyhydroxyalkanoates (PHA) is an intracellular polymer that is biologically synthesized by microorganisms as carbon and energy reserve material. This biodegradable material has properties similar to petrochemical based thermoplastics. Currently methods of PHA extraction and purification from bacterial suspension use solvent or halogenated-based approaches which are costly and pose severe environmental damage. This invention describes a combined treatment of alkaline and homogenization for extracting and purifying PHA, with higher PHA purity and recovery.
摘要翻译：聚羟基链烷酸酯（PHA）是由微生物作为碳和能量储备材料生物合成的细胞内聚合物。这种可生物降解的材料具有类似于石化基热塑性塑料的性能。目前PHA从细菌悬浮液中提取和纯化的方法使用昂贵的溶剂或卤化方法，并造成严重的环境破坏。本发明描述了用于提取和纯化PHA的碱性和均质化的组合处理，具有较高的PHA纯度和回收率。

3. 发明申请

US20080004454A1 Method for Recovering Lactide from Polylactic Acid or Derivative Thereof 失效
标题翻译：从聚乳酸或其衍生物中回收丙交酯的方法
公开(公告)号：US20080004454A1
公开(公告)日：2008-01-03
申请号：US11579144
申请日：2005-04-25
申请人： Haruo Nishida , Yujiang Fan , Yoshihito Shirai
发明人： Haruo Nishida , Yujiang Fan , Yoshihito Shirai
IPC分类号： C07D319/12
CPC分类号： C07D319/12 , C08J11/16 , C08J2367/04 , Y02W30/705
摘要： To provide an efficient method for recovering and producing lactide having high optical purity by depolymerizing a polylactic acid or derivative thereof in order to carry out chemical recycling of the polylactic acid or derivative thereof or of a resin composition comprising same, wherein a mixture of a polylactic acid or derivative thereof and aluminum hydroxide is thermally decomposed at a temperature in a range from at least the melting temperature of the polylactic acid or derivative thereof to no greater than 320° C., thus recovering lactide.
摘要翻译：为了提供一种通过解聚聚乳酸或其衍生物来回收和生产具有高光学纯度的丙交酯的有效方法，以便进行聚乳酸或其衍生物或包含其的树脂组合物的化学再循环，其中聚乳酸酸或其衍生物和氢氧化铝在至少从聚乳酸或其衍生物的熔融温度至不高于320℃的温度下热分解，从而回收丙交酯。

4. 发明申请

US20050229633A1 Process for producing concentrate by freezing and thawing and apparatus therefor 审中-公开
标题翻译：通过冷冻和解冻生产浓缩物的方法及其设备
公开(公告)号：US20050229633A1
公开(公告)日：2005-10-20
申请号：US10505817
申请日：2003-02-21
申请人： Yoshihito Shirai , Minato Wakisaka
发明人： Yoshihito Shirai , Minato Wakisaka
IPC分类号： B01D9/04 , C02F1/22
CPC分类号： B01D9/04
摘要： A process for producing a concentrate wherein a target component concentrate is obtained with low cost and in a simple manner from a solution containing a target component and unneeded suspended solid contents while unneeded suspended solid contents are removed. In particular, the process is one for obtaining a concentrate of first component from a solution containing a first component, which comprises a step of rapidly freezing the above solution to thereby obtain a frozen matter, a step of thawing all the frozen matter relatively slowly, and a first recovery step of recovering an initially thawed fraction obtained in the initial stage of thawing to thereby obtain a concentrate of first component.
摘要翻译：一种生产浓缩物的方法，其中以不需要的悬浮固体物质除去含有目标成分和不需要的悬浮固体物质的溶液，以低成本和简单的方式获得目标成分浓缩物。具体而言，该方法是从含有第一成分的溶液中获得第一成分浓缩液的方法，其特征在于，包括快速冷冻上述溶液从而获得冷冻物质的步骤，使所有冷冻物质相对缓慢地解冻的步骤，以及回收在解冻初期获得的初始解冻馏分以获得第一成分的浓缩物的第一回收步骤。

5. 发明授权

US07557224B2 Method for recovering lactide from polylactic acid or derivative thereof 失效
标题翻译：从聚乳酸或其衍生物中回收丙交酯的方法
公开(公告)号：US07557224B2
公开(公告)日：2009-07-07
申请号：US11579144
申请日：2005-04-25
申请人： Haruo Nishida , Yujiang Fan , Yoshihito Shirai
发明人： Haruo Nishida , Yujiang Fan , Yoshihito Shirai
IPC分类号： C07D323/04
CPC分类号： C07D319/12 , C08J11/16 , C08J2367/04 , Y02W30/705
摘要： To provide an efficient method for recovering and producing lactide having high optical purity by depolymerizing a polylactic acid or derivative thereof in order to carry out chemical recycling of the polylactic acid or derivative thereof or of a resin composition comprising same, wherein a mixture of a polylactic acid or derivative thereof and aluminum hydroxide is thermally decomposed at a temperature in a range from at least the melting temperature of the polylactic acid or derivative thereof to no greater than 320° C., thus recovering lactide.
摘要翻译：为了提供一种通过解聚聚乳酸或其衍生物来回收和生产具有高光学纯度的丙交酯的有效方法，以便进行聚乳酸或其衍生物或包含其的树脂组合物的化学再循环，其中聚乳酸酸或其衍生物和氢氧化铝在至少从聚乳酸或其衍生物的熔融温度至不高于320℃的温度下热分解，从而回收丙交酯。

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