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1. 发明申请

US20050260606A1 Use of whole blood in PCR reactions 审中-公开
标题翻译：在PCR反应中使用全血
公开(公告)号：US20050260606A1
公开(公告)日：2005-11-24
申请号：US10850816
申请日：2004-05-20
申请人： Milko Kermekchiev , Wayne Barnes
发明人： Milko Kermekchiev , Wayne Barnes
IPC分类号： C12N9/12 , C12P19/34 , C12Q1/68
CPC分类号： C12N9/1252
摘要： A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.
摘要翻译：一种从全血的体积获得核酸靶的DNA扩增的方法，其包括用抗血液聚合酶在PCR测定混合物中进行DNA扩增。

2. 发明申请

US20060084074A1 Use of whole blood in PCR reactions 有权
公开(公告)号：US20060084074A1
公开(公告)日：2006-04-20
申请号：US11005559
申请日：2004-12-06
申请人： Milko Kermekchiev , Wayne Barnes
发明人： Milko Kermekchiev , Wayne Barnes
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12N9/1252 , C12Q1/686 , C12Q2549/101 , C12Q2521/101
摘要： A method of obtaining DNA amplification of a nucleic acid target from a volume of whole blood comprising performing DNA amplification in a PCR assay mixture with a blood-resistant polymerase.

3. 发明授权

US09163246B2 T7 expression system 有权
标题翻译： T7表达系统
公开(公告)号：US09163246B2
公开(公告)日：2015-10-20
申请号：US12672606
申请日：2008-08-08
申请人： Wayne Barnes
发明人： Wayne Barnes
IPC分类号： C07H21/04 , C12N9/00 , C12N15/63 , C12N15/70
CPC分类号： C12N15/63 , C12N15/70
摘要： The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
摘要翻译：本发明提供改进的原核细胞表达系统，其采用严格控制的宿主菌株构建体，其控制蛋白质的未诱导的，泄漏的表达，同时仍然自动诱导。本发明的各个方面通过提供插入lac操纵子的lacZ和lacY（“ZRY”构型）的T7聚合酶基因及其突变体的宿主菌株来解决和克服未诱导的基础表达的问题，在野生型lac操纵子控制区域的下游。

4. 发明申请

US20110300576A1 T7 EXPRESSION SYSTEM 审中-公开
标题翻译： T7表达系统
公开(公告)号：US20110300576A1
公开(公告)日：2011-12-08
申请号：US12672606
申请日：2008-08-08
申请人： Wayne Barnes
发明人： Wayne Barnes
IPC分类号： C12P21/00 , C12N1/21 , C12N9/12 , C12N15/70 , C12N15/72
CPC分类号： C12N15/63 , C12N15/70
摘要： The present invention provides an improved prokaryotic cell expression system employing a tightly controlled host strain construct that controls uninduced, leaky expression of proteins while still auto-inducing well. Various aspects of the present invention address and overcome the problem of uninduced basal expression by providing a host strain that comprises a T7 polymerase gene, and mutants thereof, inserted between lac Z and lac Y of the lac operon (a “ZRY” construct), downstream of an otherwise wild-type lac operon control region.
摘要翻译：本发明提供改进的原核细胞表达系统，其采用严格控制的宿主菌株构建体，其控制蛋白质的未诱导的，泄漏的表达，同时仍然自动诱导。本发明的各个方面通过提供插入lac操纵子的lacZ和lacY（“ZRY”构型）的T7聚合酶基因及其突变体的宿主菌株来解决和克服未诱导的基础表达的问题，在野生型lac操纵子控制区域的下游。

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