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    • 2. 发明授权
    • Fusobacterium necrophorum vaccine and method for making such vaccine
    • 无菌杆菌疫苗及其制备方法
    • US06632439B2
    • 2003-10-14
    • US09408328
    • 1999-09-29
    • Adrian LiemGary A. AndersonDouglas L. Stine
    • Adrian LiemGary A. AndersonDouglas L. Stine
    • A61K4900
    • A61K39/114A61K2039/521
    • The present invention relates to a method of producing a vaccine for the prevention of F. necrophorum bacterial infections, comprising isolating the F. necrophorum bacteria from a bovine species, growing the bacteria in a suitable growth medium for a period equal to between about 10 hours and about 18 hours so as to achieve a bacterial population equal to at least 1×105 CFU/ml, terminating the growth, and using a whole cell culture to form the vaccine. Additionally, the present invention relates to the vaccine comprised of a killed whole cell population of the F. necrophorum bacteria taken from a bovine. The present invention further relates to a method for preventing footrot and liver abscesses caused by F. necrophorum bacteria.
    • 本发明涉及一种制备用于预防 F的疫苗的方法。 necrophorum 细菌感染,包括分离 F。 将细菌从牛种中生长至合适的生长培养基中等于约10小时至约18小时之间的时间,以达到等于至少1×10 5 HIL> CFU / ml,终止生长,并使用全细胞培养物形成疫苗。 另外,本发明涉及由 F的灭活全细胞群组成的疫苗。 necrophorum 从牛获取的细菌。 本发明还涉及一种防止由 F引起的脚rot和肝脓肿的方法。 necrophorum 细菌