专利快速检索-快速检索全球专利，免费商用专利数据库-IPRDB

1. 发明申请

US20120283421A1 METHODS AND REAGENTS FOR DECREASING CLINICAL REACTION TO ALLERGY 审中-公开
标题翻译：减少临床反应过敏的方法和试剂
公开(公告)号：US20120283421A1
公开(公告)日：2012-11-08
申请号：US13183892
申请日：2011-07-15
申请人： Michael J. Caplan , Howard B. Sosin , Hugh A. Sampson , Gary A. Bannon , A. Wesley Burks, JR. , Gael Cockrell , Cesar M. Compadre , Cathie Connaughton , Ricki M. Helm , Nina E. King , Randall A. Kopper , Soheila J. Maleki , Patrick A. Rabjohn , David S. Shin , J. Steven Stanley
发明人： Michael J. Caplan , Howard B. Sosin , Hugh A. Sampson , Gary A. Bannon , A. Wesley Burks, JR. , Gael Cockrell , Cesar M. Compadre , Cathie Connaughton , Ricki M. Helm , Nina E. King , Randall A. Kopper , Soheila J. Maleki , Patrick A. Rabjohn , David S. Shin , J. Steven Stanley
IPC分类号： C07K14/00
CPC分类号： C07K14/415 , A01K2217/05 , A61K38/168 , A61K39/00 , A61K2039/57 , C07K16/16 , C07K2317/34
摘要： It has been determined that allergens, which are characterized by both humoral (IgE) and cellular (T-cell) binding sites, can be modified to be less allergenic by modifying the IgE binding sites. The IgE binding sites can be converted to non-IgE binding sites by altering as little as a single amino acid within the protein, preferably a hydrophobic residue towards the center of the IgE epitope, to eliminate IgE binding. Additionally or alternatively a modified allergen with reduced IgE binding may be prepared by disrupting one or more of the disulfide bonds that are present in the natural allergen. The disulfide bonds may be disrupted chemically, e.g., by reduction and alkylation or by mutating one or more cysteine residues present in the primary amino acid sequence of the natural allergen. In certain embodiments, modified allergens are prepared by both altering one or more linear IgE epitopes and disrupting one or more disulfide bonds of the natural allergen. In certain embodiments, the methods of the present invention allow allergens to be modified while retaining the ability of the protein to activate T-cells, and, in some embodiments by not significantly altering or decreasing IgG binding capacity. The Examples provided herein use peanut allergens to illustrate applications of the invention.
摘要翻译：已经确定，通过体液（IgE）和细胞（T细胞）结合位点的特征的过敏原可以通过修饰IgE结合位点而被修饰为更低的过敏原。 IgE结合位点可以通过将蛋白质内的单个氨基酸，优选向IgE表位中心的疏水性残基改变，以消除IgE结合而转化为非IgE结合位点。另外或替代地，具有降低的IgE结合的修饰的变应原可以通过破坏天然过敏原中存在的一种或多种二硫键来制备。二硫键可以化学破坏，例如通过还原和烷基化或通过突变存在于天然过敏原的一级氨基酸序列中的一个或多个半胱氨酸残基来破坏。在某些实施方案中，通过改变一种或多种线性IgE表位并破坏天然过敏原的一个或多个二硫键来制备修饰的变应原。在某些实施方案中，本发明的方法允许改变变应原，同时保持蛋白质激活T细胞的能力，并且在一些实施方案中通过不显着改变或降低IgG结合能力。本文提供的实施例使用花生过敏原来说明本发明的应用。

2. 发明授权

US07485708B2 Nucleic acids encoding ara h 3 polypeptides 失效
标题翻译：编码ara h 3多肽的核酸
公开(公告)号：US07485708B2
公开(公告)日：2009-02-03
申请号：US10228806
申请日：2002-08-26
申请人： A. Wesley Burks, Jr. , Gary A. Bannon , Hugh A. Sampson , Ricki M. Helm , J. Steven Stanley , Patrick A. Rabjohn
发明人： A. Wesley Burks, Jr. , Gary A. Bannon , Hugh A. Sampson , Ricki M. Helm , J. Steven Stanley , Patrick A. Rabjohn
IPC分类号： C07H21/04
CPC分类号： C07K14/415 , A01K2217/05 , A61K38/00 , Y10S530/806 , Y10S530/868
摘要： It has been determined that allergens, which are characterized by both humoral (IgE) and cellular (T cell) binding sites, can be modified to be less allergenic by modifying the IgE binding sites. The IgE binding sites can be converted to non-IgE binding sites by masking the site with a compound that prevents IgE binding or by altering as little as a single amino acid within the protein, most typically a hydrophobic residue towards the center of the IgE binding epitope, to eliminate IgE binding. The method allows the protein to be altered as minimally as possible, other than within the IgE-binding sites, while retaining the ability of the protein to activate T cells, and, in some embodiments by not significantly altering or decreasing IgG binding capacity. The examples use peanut allergens to demonstrate alteration of IgE binding sites. The critical amino acids within each of the IgE binding epitopes of the peanut protein that are important to immunoglobulin binding have been determined. Substitution of even a single amino acid within each of the epitopes led to loss of IgE binding. Although the epitopes shared no common amino acid sequence motif, the hydrophobic residues located in the center of the epitope appeared to be most critical to IgE binding.
摘要翻译：已经确定，通过改变IgE结合位点，可以将以体液（IgE）和细胞（T细胞）结合位点为特征的过敏原修饰为较不敏感。通过用防止IgE结合的化合物掩蔽位点或通过改变蛋白质内的单个氨基酸，最通常的是向IgE结合中心的疏水性残基，将IgE结合位点转化为非IgE结合位点表位，以消除IgE结合。该方法允许尽可能最小化蛋白质，除了在IgE结合位点之内，同时保持蛋白质激活T细胞的能力，并且在一些实施方案中，不显着改变或降低IgG结合能力。实施例使用花生过敏原来证明IgE结合位点的改变。确定了对免疫球蛋白结合重要的花生蛋白的每个IgE结合表位内的关键氨基酸。每个表位中甚至单个氨基酸的取代导致IgE结合的丧失。尽管表位不具有共同的氨基酸序列基序，但位于该表位中心的疏水性残基似乎对IgE结合最为关键。

你已经成功收藏专利！

检索式保存成功!

IPRDB

热门服务

关于我们

友情链接

联系方式