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1. 发明授权

US10633684B2 Production of riboflavin 有权
公开(公告)号：US10633684B2
公开(公告)日：2020-04-28
申请号：US13127618
申请日：2009-11-09
申请人： Martin Lehmann , Hans-Peter Hohmann , Dietmar Laudert , Michael Hans
发明人： Martin Lehmann , Hans-Peter Hohmann , Dietmar Laudert , Michael Hans
IPC分类号： C12Q1/68 , C12P25/00 , C12N15/52 , C07K14/32
CPC分类号： C12P25/00 , C07K14/32 , C12N15/52
摘要： The present invention provides an improved biotechnological production of riboflavin (also referred herein as vitamin B2) through modification in the operon containing the riboflavin biosynthetic genes (rib operon), in particular modifications of/in the leader sequences (rib leader) upstream of the corresponding riboflavin biosynthetic genes (rib operon). Furthermore, the present invention relates to genetically engineered microorganisms carrying said modified sequences, processes to generate said modified sequences/microorganisms and the use thereof for production of riboflavin.

2. 发明申请

US20080311617A1 Modified Transketolase and Use Thereof 有权
标题翻译：改良转酮醇酯及其用途
公开(公告)号：US20080311617A1
公开(公告)日：2008-12-18
申请号：US12091279
申请日：2006-10-25
申请人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
发明人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
IPC分类号： C12P25/00 , C12N1/21
CPC分类号： C12N9/1022 , C12P17/12 , C12P25/00
摘要： The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
摘要翻译：本发明涉及核糖-5-磷酸，核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体如核黄素（维生素B2），FAD，FMN，吡哆醛磷酸酯（维生素B6），鸟苷，GMP，腺苷，AMP。本发明还涉及产生这些化合物的生物体的产生。此外，还涉及产生突变的转酮酶，其允许在葡萄糖上正常生长，但当被引入生产菌株中时葡萄糖酸盐的生长减少，以及编码它们的多核苷酸。

3. 发明申请

US20130189731A1 MODIFIED TRANSKETOLASE AND USE THEREOF 审中-公开
标题翻译：改性的转铁蛋白酶及其用途
公开(公告)号：US20130189731A1
公开(公告)日：2013-07-25
申请号：US13558517
申请日：2012-07-26
申请人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
发明人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
IPC分类号： C12N9/10 , C12P17/12 , C12P25/00
CPC分类号： C12N9/1022 , C12P17/12 , C12P25/00
摘要： The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
摘要翻译：本发明涉及核糖-5-磷酸，核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体如核黄素（维生素B2），FAD，FMN，吡哆醛磷酸酯（维生素B6），鸟苷，GMP，腺苷，AMP。本发明还涉及产生这些化合物的生物体的产生。此外，还涉及允许在葡萄糖上正常生长但在引入生产菌株中时降低葡萄糖酸盐生长并且编码它们的多核苷酸的突变转酮酶的产生。

4. 发明申请

US20110312025A1 IMPROVED PRODUCTION OF RIBOFLAVIN 审中-公开
标题翻译：改善RIBOFLAVIN的生产
公开(公告)号：US20110312025A1
公开(公告)日：2011-12-22
申请号：US13127618
申请日：2009-11-09
申请人： Martin Lehmann , Hans-Peter Hohmann , Dietmar Laudert , Michael Hans
发明人： Martin Lehmann , Hans-Peter Hohmann , Dietmar Laudert , Michael Hans
IPC分类号： C12P25/00 , C12P19/34 , C12N15/74 , C12N15/75 , C12N15/115 , C12N1/21
CPC分类号： C12P25/00 , C07K14/32 , C12N15/52
摘要： The present invention provides an improved biotechnological production of riboflavin (also referred herein as vitamin B2) through modification in the operon containing the riboflavin biosynthetic genes (rib operon), in particular modifications of/in the leader sequences (rib leader) upstream of the corresponding riboflavin biosynthetic genes (rib operon). Furthermore, the present invention relates to genetically engineered microorganisms carrying said modified sequences, processes to generate said modified sequences/microorganisms and the use thereof for production of riboflavin.
摘要翻译：本发明通过在含有核黄素生物合成基因（rib操纵子）的操纵子中进行修饰，特别是在对应的上游的前导序列（rib前导序列）中的修饰来提供核黄素（本文中也称为维生素B2）的改进的生物技术生产核黄素生物合成基因（rib操纵子）。此外，本发明涉及携带所述修饰序列的遗传工程微生物，产生所述修饰序列/微生物的方法及其用于生产核黄素的用途。

5. 发明授权

US09315784B2 Modified transketolase and use thereof 有权
标题翻译：改性转酮酶及其用途
公开(公告)号：US09315784B2
公开(公告)日：2016-04-19
申请号：US13558517
申请日：2012-07-26
申请人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
发明人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
IPC分类号： C12P17/18 , C12N9/10 , C12P25/00 , C12P17/12
CPC分类号： C12N9/1022 , C12P17/12 , C12P25/00
摘要： The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
摘要翻译：本发明涉及核糖-5-磷酸，核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体如核黄素（维生素B2），FAD，FMN，吡哆醛磷酸酯（维生素B6），鸟苷，GMP，腺苷，AMP。本发明还涉及产生这些化合物的生物体的产生。此外，还涉及产生突变的转酮酶，其允许在葡萄糖上正常生长，但当被引入生产菌株中时葡萄糖酸盐的生长减少，以及编码它们的多核苷酸。

6. 发明授权

US08257944B2 Modified transketolase and use thereof 有权
标题翻译：改性转酮酶及其用途
公开(公告)号：US08257944B2
公开(公告)日：2012-09-04
申请号：US12091279
申请日：2006-10-25
申请人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
发明人： Michael Hans , Dietmar Laudert , Hans-Peter Hohmann , Martin Lehmann
IPC分类号： C12P25/00 , C12N1/20 , C12N9/10 , C07D491/00
CPC分类号： C12N9/1022 , C12P17/12 , C12P25/00
摘要： The present invention relates to a improved process for the biotechnological production of compounds for which ribose-5-phosphate, ribulose-5-phosphate or xylulose-5-phosphate is biosynthetic precursor like riboflavin (vitamin B2), FAD, FMN, pyridoxal phosphate (vitamin B6), guanosine, GMP, adenosine, AMP. The invention further pertains to the generation of the organism producing those compounds. It furthermore relates to the generation of mutated transketolases that allow normal growth on glucose but reduced growth on gluconate when introduced into the production strains and to polynucleotides encoding them.
摘要翻译：本发明涉及核糖-5-磷酸，核酮糖-5-磷酸或木酮糖-5-磷酸是生物合成前体如核黄素（维生素B2），FAD，FMN，吡哆醛磷酸酯（维生素B6），鸟苷，GMP，腺苷，AMP。本发明还涉及产生这些化合物的生物体的产生。此外，它还涉及允许葡萄糖正常生长但在引入生产菌株中时减少葡萄糖酸盐生长并且编码它们的多核苷酸的突变转酮酶的产生。

7. 发明授权

US09624521B2 Production of non-yeast sterols by yeast 有权
公开(公告)号：US09624521B2
公开(公告)日：2017-04-18
申请号：US13510636
申请日：2010-11-24
申请人： Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
发明人： Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
IPC分类号： C12N9/02 , C12N9/10 , C12N1/19 , C12P33/06 , C12P33/00
CPC分类号： C12P33/00 , C12N9/001 , C12N9/0067 , C12N9/0071 , C12N9/0083 , C12N9/1007 , C12P33/06
摘要： This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as Saccharomyces cerevisiae. It also relates to various enzymes catalyzing the reduction of the double bond at position 24 of lanosterol, dimethyl zymosterol, methyl zymosterol, zymosterol, cholesta-7, 24-dienol, or cholesta-5,7,24-trienol; or the hydroxylation at position 25 of ergosterol, 7-dehydrocholesterol, cholesta-8-enol, and cholesta-7-enol. It also relates to various nucleic acids encoding cholesterol C25-hydroxylases and sterol Δ24-reductases and their use to produce and hydroxylate 7-dehydrocholesterol or ergosterol. It also relates to the yeast strains so produced, and methods of making these sterols that include the steps of cultivaton a transformed yeast cell, and harvesting the resulting sterol(s).

8. 发明申请

US20120231495A1 PRODUCTION OF NON-YEAST STEROLS BY YEAST 有权
标题翻译： YEAST生产非YEAST STEROLS
公开(公告)号：US20120231495A1
公开(公告)日：2012-09-13
申请号：US13510636
申请日：2010-11-24
申请人： Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
发明人： Hans-Peter Hohmann , Martin Lehmann , Muriel Merkamm , Denis Pompon
IPC分类号： C12P33/06 , C12N15/53 , C12N15/81 , C12N1/19
CPC分类号： C12P33/00 , C12N9/001 , C12N9/0067 , C12N9/0071 , C12N9/0083 , C12N9/1007 , C12P33/06
摘要： This invention relates to the production of 7-dehydrocholesterol, 25-hydroxy-7-dehydrocholesterol, and 25-hydroxy ergosterol in yeast such as Saccharomyces cerevisiae. It also related to various enzymes catalyzing the reduction of the double bond at position 24 of lanosterol, dimethyl zymosterol, methyl zymosterol, zymosterol, cholesta-7, 24-dienol, or cholesta-5,7,24-trienol; or the hydroxylation at position 25 of ergosterol, 7-dehydrocholesterol, cholesta-8-enol, and cholesta-7-enol. It also relates to various nucleic acids encoding cholesterol C25-hydroxylases and sterol Δ24-reductases and their use to produce and hydroxylate 7-dehydrocholesterol or ergosterol. It also relates to the yeast strains so produced, and methods of making these sterols comprising the steps of cultivation a transformed yeast cell, and harvesting the resulting sterol(s).
摘要翻译：本发明涉及酵母如酿酒酵母中7-脱氢胆固醇，25-羟基-7-脱氢胆固醇和25-羟基麦角甾醇的生产。它还涉及催化羊毛甾醇，二甲基十八烷醇，甲基十八醇，十八酮，胆甾烯-7,24-二烯醇或胆石酯-5,7,24-三烯酚在位置24处双键还原的各种酶。或麦角甾醇，7-脱氢胆固醇，胆甾烯-8-烯醇和胆甾烯-7-烯醇在25位的羟基化。它还涉及编码胆固醇C25-羟化酶和甾醇和Dgr; 24-还原酶的各种核酸，以及它们用于产生7-羟基胆固醇或麦角固醇羟基化的用途。它还涉及如此制备的酵母菌株，以及制备这些甾醇的方法包括培养转化的酵母细胞的步骤，并收获所得的甾醇。

9. 发明授权

US07695949B2 Process for producing a target fermentation product 有权
标题翻译：生产靶发酵产物的方法
公开(公告)号：US07695949B2
公开(公告)日：2010-04-13
申请号：US10681086
申请日：2003-10-08
申请人： Hans-Peter Hohmann , Nigel John Mouncey , Heinrich Winfred Schlieker , Jeffrey W. Stebbins
发明人： Hans-Peter Hohmann , Nigel John Mouncey , Heinrich Winfred Schlieker , Jeffrey W. Stebbins
IPC分类号： C12N1/20 , C07H21/04
CPC分类号： C12P25/00 , C12P13/02 , C12P13/04 , C12P17/182
摘要： The present invention provides a process for producing a target fermentation product. This process includes providing a fermentation medium containing a recombinantly-produced microorganism that over-produces a fermentation product and contains a mutation which causes auxotrophic growth of the microorganism wherein the auxotrophy within the microorganism does not compromise the ability of the microorganism to produce the fermentation product. The medium is then supplied in excess with all substrates required for production of the fermentation product and in growth limiting amounts with a substrate complementing the auxotrophy. Host cells, vectors, and polynucleotide sequences used in the process are also provided. The polynucleotide sequences of the present invention include sequences derived from the biotin operon of B. subtilis and in particular the bioFDB gene cassette.
摘要翻译：本发明提供了生产靶发酵产物的方法。该方法包括提供含有重组产生的微生物的发酵培养基，其过量产生发酵产物并含有导致微生物营养缺陷型生长的突变，其中微生物内的营养缺陷不损害微生物产生发酵产物的能力。然后将培养基过量供给用于生产发酵产物所需的所有底物，并且与补充营养缺陷型的底物一起生长限制量。还提供了用于该方法的宿主细胞，载体和多核苷酸序列。本发明的多核苷酸序列包括衍生自枯草芽孢杆菌的生物素操纵子，特别是bioFDB基因盒的序列。

10. 发明授权

US6087152A Fermentative carotenoid production 失效
标题翻译：发酵类胡萝卜素生产
公开(公告)号：US6087152A
公开(公告)日：2000-07-11
申请号：US660645
申请日：1996-06-07
申请人： Hans-Peter Hohmann , Luis Pasamontes , Michel Tessier , Adolphus van Loon
发明人： Hans-Peter Hohmann , Luis Pasamontes , Michel Tessier , Adolphus van Loon
IPC分类号： A23K1/16 , A23L1/30 , C07H21/04 , C07K14/195 , C12N1/21 , C12N15/09 , C12N15/52 , C12N15/53 , C12P7/04 , C12P23/00 , C12R1/05 , C12R1/125 , C12R1/19 , C12R1/20 , C12N1/20
CPC分类号： C12N15/52 , C07K14/195 , C12P23/00
摘要： Novel proteins of Flavobacterium sp. R1534 and the DNA sequences which encode these proteins are disclosed which provide an improved biosynthetic pathway from farnesyl pyrophosphate and isopentyl pyrophosphate to various carotenoid precursors and carotenoids, especially .beta.-carotene, lycopene, zeaxanthin and cantaxanthin.
摘要翻译：黄杆菌新蛋白公开了编码这些蛋白质的DNA序列，其提供了从法呢基焦磷酸盐和异戊基焦磷酸盐到各种类胡萝卜素前体和类胡萝卜素，特别是β-胡萝卜素，番茄红素，玉米黄质和角质黄素的改进的生物合成途径。

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