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1. 发明授权

US07989679B2 Methods and compositions for improved enzyme activity in transgenic plants 失效
标题翻译：在转基因植物中改善酶活性的方法和组合物
公开(公告)号：US07989679B2
公开(公告)日：2011-08-02
申请号：US11681285
申请日：2007-03-02
申请人： Michael G. Koziel , Laura Cooper Schouten , Brian Vande Berg
发明人： Michael G. Koziel , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/82
CPC分类号： C12N15/8261 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： Compositions and methods for increasing enzyme activity across a broad physiological spectrum in plants, plant cells, tissues and seeds are provided. Compositions include plants or plant parts comprising two or more polynucleotides encoding polypeptides that are active across a broader physiological spectrum than when either polynucleotide is expressed alone. Vectors comprising these polynucleotide molecules as well as host cells comprising the vectors are further provided. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In addition, methods are provided for producing the plants, plant cells, tissues and seeds of the invention. Methods for increasing plant yield and methods for conferring resistance to an herbicide in a plant are further provided.
摘要翻译：提供了用于增加植物，植物细胞，组织和种子中广泛生理光谱的酶活性的组合物和方法。组合物包括植物或植物部分，其包含两个或更多个编码与更多生理光谱相关的多肽的多核苷酸，而不是单个表达。还提供了包含这些多核苷酸分子的载体以及包含载体的宿主细胞。组合物还包括转化的细菌，植物，植物细胞，组织和种子。此外，提供了用于生产本发明的植物，植物细胞，组织和种子的方法。还提供了增加植物产量的方法和赋予植物中除草剂抗性的方法。

2. 发明授权

US08247535B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US08247535B2
公开(公告)日：2012-08-21
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/88 , A61K38/43 , A61K380/00
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

3. 发明授权

US07960616B2 Directed evolution of GRG31 EPSP synthase enzyme 有权
标题翻译： GRG31 EPSP合成酶的定向进化
公开(公告)号：US07960616B2
公开(公告)日：2011-06-14
申请号：US12362774
申请日：2009-01-30
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N15/82 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

4. 发明授权

US08450267B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08450267B2
公开(公告)日：2013-05-28
申请号：US12856082
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C07K14/00 , C12N15/11
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:9, 11, 13, 15, 17, 19, 21, 23, 25, 27, 29, 31, 33, or 35, or the nucleotide sequence set forth in SEQ ID NO:6, 8, 10, 12, 14, 16, 18, 20, 22, 24, 26, 28, 30, 32, or 34.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了分离的多核苷酸，其含有编码SEQ ID NO：9,11,13,15,17,19,21,23,25,27,29,31,33，或35，或SEQ ID NO：6,8,10,12,14,16,18,20,22,24,26,28,30,32或34所示的核苷酸序列。

5. 发明授权

US08283523B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08283523B2
公开(公告)日：2012-10-09
申请号：US12856072
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。

6. 发明授权

US08252981B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252981B2
公开(公告)日：2012-08-28
申请号：US12856058
申请日：2010-08-13
申请人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
发明人： Laura Cooper Schouten , Brian Vande Berg , Volker Heinrichs
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 33, or the nucleotide sequence set forth in SEQ ID NO: 32.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：33所示的氨基酸序列的核苷酸序列或SEQ ID NO：32所示的核苷酸序列的分离的多核苷酸。

7. 发明申请

US20110223648A1 DIRECTED EVOLUTION OF GRG31 EPSP SYNTHASE ENZYME 有权
标题翻译： GRG31 EPSP合成酶的指导进化
公开(公告)号：US20110223648A1
公开(公告)日：2011-09-15
申请号：US13101351
申请日：2011-05-05
申请人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
发明人： Volker Heinrichs , Laura Cooper Schouten , Brian Vande Berg
IPC分类号： C12N9/10
CPC分类号： C12N15/8275 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided, particularly polypeptide variants of SEQ ID NO:2 and 4. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:7-28, or the nucleotide sequence set forth in SEQ ID NO:29 or 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码抗草甘膦抗性或耐受性多肽的分离的多核苷酸，特别是SEQ ID NO：2和4的多肽变体。此外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：7-28所示的氨基酸序列的核苷酸序列或SEQ ID NO：29或30所示的核苷酸序列的分离的多核苷酸。

8. 发明授权

US08309332B2 GRG23 and GRG51 polypeptides having herbicide resistance activity 有权
标题翻译：具有除草剂抗性活性的GRG23和GRG51多肽
公开(公告)号：US08309332B2
公开(公告)日：2012-11-13
申请号：US12468205
申请日：2009-05-19
申请人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Burdette Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N9/00
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

9. 发明授权

US08252980B2 GRG23 EPSP synthases: compositions and methods of use 有权
标题翻译： GRG23 EPSP合成酶：组合物和使用方法
公开(公告)号：US08252980B2
公开(公告)日：2012-08-28
申请号：US12856021
申请日：2010-08-13
申请人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
发明人： Laura Cooper Schouten , Cheryl L. Peters , Brian Vande Berg
IPC分类号： C12N1/21 , C12N15/52 , C12N15/82
CPC分类号： C12N15/8275 , C12N9/1085 , C12N9/1092
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also include transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides containing nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO: 31, or the nucleotide sequence set forth in SEQ ID NO: 30.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了含有编码SEQ ID NO：31所示的氨基酸序列的核苷酸序列或SEQ ID NO：30所示的核苷酸序列的分离的多核苷酸。

10. 发明授权

US07674958B2 GRG23 and GRG51 genes conferring herbicide resistance 有权
标题翻译：赋予除草剂抗性的GRG23和GRG51基因
公开(公告)号：US07674958B2
公开(公告)日：2010-03-09
申请号：US11605824
申请日：2006-11-29
申请人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
发明人： Cheryl L. Peters , Jill Hinson , Philip E. Hammer , Brian Vande Berg , Laura Cooper Schouten , Brian Carr
IPC分类号： C12N15/82 , C12N15/63 , C12N15/52
CPC分类号： C12N15/8275 , C07K14/195 , C07K14/415 , C12N15/8274 , C12Q1/42
摘要： Compositions and methods for conferring herbicide resistance or tolerance to bacteria, plants, plant cells, tissues and seeds are provided. Compositions include polynucleotides encoding herbicide resistance or tolerance polypeptides, vectors comprising those polynucleotides, and host cells comprising the vectors. The nucleotide sequences of the invention can be used in DNA constructs or expression cassettes for transformation and expression in organisms, including microorganisms and plants. Compositions also comprise transformed bacteria, plants, plant cells, tissues, and seeds. In particular, isolated polynucleotides encoding glyphosate resistance or tolerance polypeptides are provided. Additionally, amino acid sequences corresponding to the polynucleotides are encompassed. In particular, the present invention provides for isolated polynucleotides comprising nucleotide sequences encoding the amino acid sequence shown in SEQ ID NO:2, 4, or 6, or the nucleotide sequence set forth in SEQ ID NO:1, 3, or 5. The present invention additionally provides a method to measure enzyme kinetic activity using fluorogenic substrates.
摘要翻译：提供了赋予除草剂抗性或对细菌，植物，植物细胞，组织和种子的耐受性的组合物和方法。组合物包括编码除草剂抗性或耐受性多肽的多核苷酸，包含那些多核苷酸的载体和包含载体的宿主细胞。本发明的核苷酸序列可用于DNA构建体或表达盒中用于在生物体（包括微生物和植物）中转化和表达。组合物还包括转化的细菌，植物，植物细胞，组织和种子。特别地，提供了编码草甘膦抗性或耐受性多肽的分离的多核苷酸。另外，包括对应于多核苷酸的氨基酸序列。特别地，本发明提供了包含编码SEQ ID NO：2,4或6所示的氨基酸序列的核苷酸序列或SEQ ID NO：1,3或5所示的核苷酸序列的分离的多核苷酸。本发明另外提供了使用荧光底物测量酶动力学活性的方法。

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