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1. 发明授权

US5891859A Method for regulating cold and dehydration regulatory genes in a plant 失效
标题翻译：调节植物中冷和脱水调节基因的方法
公开(公告)号：US5891859A
公开(公告)日：1999-04-06
申请号：US949603
申请日：1997-10-14
申请人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Daniel Zarka , Sarah Jane Gilmour
发明人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Daniel Zarka , Sarah Jane Gilmour
IPC分类号： C07K14/395 , C07K14/415 , C12N15/12 , C12N15/82 , C07H21/04 , C07K1/00
CPC分类号： C12N15/8273 , C07K14/395 , C07K14/415
摘要： A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.
摘要翻译：描述了编码蛋白质CBF1的基因，CBF1结合调节植物中冷温度和脱水耐受性的基因表达的区域。 CBF1用于转化微生物，可用于转化植物。

2. 发明授权

US06417428B1 Plant having altered environmental stress tolerance 有权
标题翻译：植物具有改变的环境胁迫耐受性
公开(公告)号：US06417428B1
公开(公告)日：2002-07-09
申请号：US09198119
申请日：1998-11-23
申请人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Sarah Jane Gilmour , Daniel Zarka , Cai-Zhong Jiang
发明人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Sarah Jane Gilmour , Daniel Zarka , Cai-Zhong Jiang
IPC分类号： A01H100
CPC分类号： C07K14/395 , C07K14/415 , C12N15/8216 , C12N15/8222 , C12N15/8237 , C12N15/8273
摘要： A transformed plant is provided which comprises one or more environmental stress tolerance genes; a DNA regulatory sequence which regulates expression of the one or more environmental stress tolerance genes; a sequence encoding a binding protein capable of binding to the DNA regulatory sequence and inducing expression of the one or more environmental stress tolerance genes; and a recombinant promoter which regulates expression of the gene encoding the binding protein. A method for altering an environmental stress tolerance of a plant is also provided which comprises the steps of transforming a plant with a promoter which regulates expression of at least one copy of a gene encoding a binding protein capable of binding to a DNA regulatory sequence which regulates one or more environmental stress tolerance genes in the plant; expressing the binding protein encoded by the gene; and stimulating expression of at least one environmental stress tolerance gene through binding of the binding protein to the DNA regulatory sequence.
摘要翻译：提供了包含一个或多个环境胁迫耐受基因的转化植物; 调节一个或多个环境胁迫耐受基因表达的DNA调控序列; 编码能够结合DNA调节序列并诱导一种或多种环境胁迫耐受基因的表达的结合蛋白的序列; 以及调节编码结合蛋白的基因表达的重组启动子。还提供了一种用于改变植物的环境胁迫耐受性的方法，其包括用启动子转化植物的步骤，所述启动子调节至少一个拷贝的表达，该拷贝编码能够结合调节DNA调节序列的结合蛋白植物中一个或多个环境胁迫耐受基因; 表达由该基因编码的结合蛋白; 并通过结合蛋白质与DNA调节序列结合来刺激至少一种环境胁迫耐受基因的表达。

3. 发明授权

US5892009A DNA and encoded protein which regulates cold and dehydration regulated genes 失效
标题翻译： DNA和编码的蛋白质调节冷和脱水调节基因
公开(公告)号：US5892009A
公开(公告)日：1999-04-06
申请号：US706270
申请日：1996-09-04
申请人： Michael F. Thomashow , Eric J. Stockinger
发明人： Michael F. Thomashow , Eric J. Stockinger
IPC分类号： C07K14/395 , C07K14/415 , C12N15/12 , C12N15/82 , C07H21/02 , A01N37/18 , C07K1/00
CPC分类号： C12N15/8273 , C07K14/395 , C07K14/415
摘要： A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.
摘要翻译：描述了编码蛋白质CBF1的基因，CBF1结合调节植物中冷温度和脱水耐受性的基因表达的区域。 CBF1用于转化微生物，可用于转化植物。

4. 发明授权

US5965705A DNA and encoded protein which regulates cold and dehydration regulated genes 失效
公开(公告)号：US5965705A
公开(公告)日：1999-10-12
申请号：US950172
申请日：1997-10-14
申请人： Michael F. Thomashow , Eric J. Stockinger
发明人： Michael F. Thomashow , Eric J. Stockinger
IPC分类号： C07K14/395 , C07K14/415 , C12N15/12 , C12N15/82 , C07K1/00 , A01H1/04 , A01N43/04
CPC分类号： C12N15/8273 , C07K14/395 , C07K14/415
摘要： A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.

5. 发明授权

US5929305A Plant material containing non-naturally introduced binding protein for regulating cold and dehydration regulatory genes 失效
标题翻译：含有非天然引入的结合蛋白的植物材料用于调节冷和脱水调节基因
公开(公告)号：US5929305A
公开(公告)日：1999-07-27
申请号：US949580
申请日：1997-10-14
申请人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Daniel Zarka
发明人： Michael F. Thomashow , Eric J. Stockinger , Kirsten Jaglo-Ottosen , Daniel Zarka
IPC分类号： C07K14/415 , C12N15/19 , C12N15/82 , A01H5/00 , C12N5/04
CPC分类号： C12N15/8273 , C07K14/415
摘要： A gene, designated as CBF1, encoding a protein, CBF1, which binds to a region regulating expression of genes which promote cold temperature and dehydration tolerance in plants is described. CBF1 is used to transform microorganisms and can be used to transform plants.
摘要翻译：描述了编码蛋白质CBF1的基因，CBF1结合调节植物中冷温度和脱水耐受性的基因表达的区域。 CBF1用于转化微生物，可用于转化植物。

6. 发明授权

US06706866B1 Plant having altered environmental stress tolerance 有权
标题翻译：植物具有改变的环境胁迫耐受性
公开(公告)号：US06706866B1
公开(公告)日：2004-03-16
申请号：US09601802
申请日：2000-09-15
申请人： Michael Thomashow , Sarah Jane Gilmour , Cai-Zhong Jiang , Michael Fromm , Daniel Zarka , Kirsten Jaglo-Ottosen , Eric J. Stockinger , James Zhang , Volker Haake
发明人： Michael Thomashow , Sarah Jane Gilmour , Cai-Zhong Jiang , Michael Fromm , Daniel Zarka , Kirsten Jaglo-Ottosen , Eric J. Stockinger , James Zhang , Volker Haake
IPC分类号： C07H2100
CPC分类号： C12N15/8216 , C07K14/395 , C07K14/415 , C12N15/8222 , C12N15/8237 , C12N15/8273
摘要： The present invention provides for a non-naturally occurring binding protein comprising an amino acid sequence capable of binding to a CCG regulatory sequence and an amino acid sequence which forms a transcription activation region. In particular the invention comprises a sequence selected from the group consisting of an AP2 domain of SEQ. I.D. Nos. 13, 15, 39, 41, 43, 45, 47, 49, 51, 53, 55, 57, 59, 61, 63, 65, 67, 69, 71, 73, 75, 77, 79, 81, 83, 85, 87, 89, 91, 93, and 95. When these proteins are overexpressed in a plant the plant exhibits increased tolerance to environmental stresses such as cold, freezing, drought or high salinity.
摘要翻译：本发明提供了非天然存在的结合蛋白，其包含能够结合CCG调节序列的氨基酸序列和形成转录活化区的氨基酸序列。特别地，本发明包括选自SEQ ID NO：2的AP2结构域的序列。 ID。第13号，第15号，第39条，第41条，第43条，第45条，第47条，第49条，第51条，第53条，第55条，第57条，第59条，第61条，第63条，第65条，第67条，第69条，第71条，第73条，第75条， 83,85,87,89,91,93和95.当这些蛋白质在植物中过表达时，植物对环境胁迫如冷，冷冻，干旱或高盐度表现出增加的耐受性。

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