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1. 发明授权

US08993294B2 Cross-flow membrane filtration-based process for protein recovery 有权
标题翻译：基于过滤膜过滤的蛋白质回收方法
公开(公告)号：US08993294B2
公开(公告)日：2015-03-31
申请号：US13389251
申请日：2010-08-10
申请人： Michael Bodo , Meng H. Heng
发明人： Michael Bodo , Meng H. Heng
IPC分类号： C12N9/00 , C12P21/06 , B01D61/14 , B01D61/16 , B01D61/22 , C07K14/37
CPC分类号： B01D61/142 , B01D61/16 , B01D61/22 , B01D2311/04 , B01D2311/16 , B01D2311/18 , B01D2311/2642 , B01D2315/14 , B01D2315/16 , B01D2317/022 , C07K14/37 , B01D2311/14 , B01D2311/25
摘要： The present methods relate to the isolation and concentration of proteins using cross-flow membrane filtration, and to proteins produced by such methods. A feature of the method is that the protein of interest is retained by a cross-flow membrane under certain conditions that promote retention, while under other condition the protein passes through the membrane.
摘要翻译：本方法涉及使用交叉膜过滤的蛋白质的分离和浓缩以及通过这些方法产生的蛋白质。该方法的特征在于，在促进保留的某些条件下，交叉流动膜保留目的蛋白质，而在其它条件下，蛋白质通过膜。

2. 发明申请

US20120252066A1 METHODS OF FOAM CONTROL 审中-公开
标题翻译：泡沫控制方法
公开(公告)号：US20120252066A1
公开(公告)日：2012-10-04
申请号：US13433036
申请日：2012-03-28
申请人： Meng H. Heng , Michael Bodo
发明人： Meng H. Heng , Michael Bodo
IPC分类号： C12P21/00 , C12P19/44
CPC分类号： C12P19/44 , C07K14/37 , C12P7/64
摘要： The invention relates to a method for decreasing foam formation as well as maximizing expression of a biosurfactant in a microorganism. The methods encompasses precipitating a biosurfactant from the microorganism which results in decreased form formation.
摘要翻译：本发明涉及减少泡沫形成以及使微生物中生物表面活性剂表达最大化的方法。所述方法包括从微生物中沉淀生物表面活性剂，导致形成形成减少。

3. 发明申请

US20120220009A1 Cross-Flow Membrane Filtration-Based Process For Protein Recovery 有权
标题翻译：基于跨流膜过滤的蛋白质回收方法
公开(公告)号：US20120220009A1
公开(公告)日：2012-08-30
申请号：US13389251
申请日：2010-08-10
申请人： Michael Bodo , Meng H. Heng
发明人： Michael Bodo , Meng H. Heng
IPC分类号： C07K1/34 , C07K1/36 , C12N9/00 , C07K14/37
CPC分类号： B01D61/142 , B01D61/16 , B01D61/22 , B01D2311/04 , B01D2311/16 , B01D2311/18 , B01D2311/2642 , B01D2315/14 , B01D2315/16 , B01D2317/022 , C07K14/37 , B01D2311/14 , B01D2311/25
摘要： The present methods relate to the isolation and concentration of proteins using cross-flow membrane filtration, and to proteins produced by such methods. A feature of the method is that the protein of interest is retained by a cross-flow membrane under certain conditions that promote retention, while under other condition the protein passes through the membrane.
摘要翻译：本方法涉及使用交叉膜过滤的蛋白质的分离和浓缩以及通过这些方法产生的蛋白质。该方法的特征在于，在促进保留的某些条件下，交叉流动膜保留目的蛋白质，而在其它条件下，蛋白质通过膜。

4. 发明申请

US20110189344A1 RECOVERY OF INSOLUBLE ENZYME FROM FERMENTATION BROTH AND FORMULATION OF INSOLUBLE ENZYME 审中-公开
标题翻译：从发酵中恢复不溶性酵素和制备不溶性酶
公开(公告)号：US20110189344A1
公开(公告)日：2011-08-04
申请号：US13062480
申请日：2009-06-09
申请人： Michael Bodo , Robert I. Christensen , Rajdeep S. Dhaliwal , Meng H. Heng
发明人： Michael Bodo , Robert I. Christensen , Rajdeep S. Dhaliwal , Meng H. Heng
IPC分类号： A23L1/30 , C12N9/00 , C12N9/98 , D06M16/00 , C11D3/386 , A23K1/165
CPC分类号： C12N9/98 , A23K20/189 , A23K40/10 , A23K40/30 , A23L29/06 , C11D3/38609 , C11D3/38672 , C12N9/00 , C12N9/2417 , C12N9/2428 , C12N9/54 , C12Y302/01001 , C12Y302/01003 , C12Y304/21062
摘要： Methods are provided for recovery and formulation of insoluble enzymes from a microbial fermentation broth, without removal of microbial cells or cell debris. Granular and liquid formulations comprising insoluble enzymes are also provided.
摘要翻译：提供了用于从微生物发酵液中回收和配制不溶性酶的方法，而不去除微生物细胞或细胞碎片。还提供了包含不溶性酶的颗粒和液体制剂。

5. 发明授权

US07626004B2 Protein purification 有权
标题翻译：蛋白质纯化
公开(公告)号：US07626004B2
公开(公告)日：2009-12-01
申请号：US11717510
申请日：2007-03-13
申请人： Robin Fong , Meng H. Heng
发明人： Robin Fong , Meng H. Heng
IPC分类号： C07K1/16
CPC分类号： C07K16/06 , C07K2317/55
摘要： The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatography (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.
摘要翻译：本发明涉及从其融合类似物纯化目的蛋白质的方法，包括获得包含目的蛋白质及其融合类似物的蛋白质溶液; 用合适的缓冲液调节蛋白质溶液的pH和/或离子强度，以与疏水电荷诱导色谱（HCIC）树脂一起使用，使蛋白质溶液和HCIC树脂柱接触，以使目标蛋白和其融合类似物，洗涤树脂并通过pH梯度从树脂中洗脱目的蛋白质; 其中所述目标蛋白基本上不含其融合类似物。

6. 发明授权

US07374684B2 Method of separating components in a sample using silane-treated silica filter media 有权
标题翻译：使用硅烷处理的二氧化硅过滤介质分离样品中组分的方法
公开(公告)号：US07374684B2
公开(公告)日：2008-05-20
申请号：US11828275
申请日：2007-07-25
申请人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
发明人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
IPC分类号： B01D37/00
CPC分类号： B01J20/3259 , B01D15/361 , B01D15/3804 , B01J20/103 , B01J20/14 , B01J20/286 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3257 , B01J2220/4887 , Y10T436/25375
摘要： The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.
摘要翻译：本发明提供了从含有微粒和可溶性材料的样品中分离出一种或多种感兴趣组分的方法。该方法包括以下步骤：（a）通过其表面硅烷醇基团与一种或多种硅烷反应的二氧化硅过滤介质过滤样品，和（b）同时捕获微粒并将可溶组分结合到二氧化硅过滤介质上。随后从二氧化硅过滤介质中洗脱所结合的可溶性成分。在本发明的一个实施方案中，不希望的可溶物质被经处理的二氧化硅过滤介质捕获，并且所需的目的组分从流通中回收。在另一个实施方案中，感兴趣的不同成分从洗脱液和流通中回收。优选的经处理的二氧化硅过滤介质是具有功能季铵基或功能磺酸盐基团的硅烷处理的稻壳灰或硅藻土。适用于本发明的微粒例如是微生物。

7. 发明授权

US06593118B2 Method for rapidly obtaining crystals with desirable morphologies 有权
公开(公告)号：US06593118B2
公开(公告)日：2003-07-15
申请号：US10053199
申请日：2001-11-02
申请人： Meng H. Heng
发明人： Meng H. Heng
IPC分类号： C12N9100
CPC分类号： C12N9/54 , C07K1/306 , C30B7/00 , C30B29/58 , Y10S435/814 , Y10S435/816
摘要： The present invention provides a crystallization process wherein a starting temperature is selected such that a desirable crystal morphology (e.g., square) is obtained. A temperature shift is then introduced, providing that the shift is not enough to induce further nucleation, where the crystals continue to grow in the desirable fashion, but with different kinetics, e.g., a higher rate of crystallization. As a result, the process gives a crystalline product with desirable morphology at a higher crystallization rate.

8. 发明授权

US07208585B2 Protein purification 有权
标题翻译：蛋白质纯化
公开(公告)号：US07208585B2
公开(公告)日：2007-04-24
申请号：US10663483
申请日：2003-09-15
申请人： Robin Fong , Meng H. Heng
发明人： Robin Fong , Meng H. Heng
IPC分类号： C07K1/16
CPC分类号： C07K16/06 , C07K2317/55
摘要： The present invention relates to a method of purifying a protein of interest from its fusion analog, comprising obtaining a protein solution comprising a protein of interest and its fusion analog; adjusting the pH and/or ionic strength of the protein solution with an appropriate buffer for use with a Hydrophobic Charge Induction Chromatograph (HCIC) resin, contacting the protein solution and HCIC resin column to allow binding of the protein of interest and its fusion analog, washing the resin and eluting the protein of interest from the resin by a pH gradient; wherein the protein of interest is substantially free of its fusion analog.
摘要翻译：本发明涉及从其融合类似物纯化目的蛋白质的方法，包括获得包含目的蛋白质及其融合类似物的蛋白质溶液; 用合适的缓冲液调节蛋白质溶液的pH和/或离子强度，以与疏水电荷诱导色谱（HCIC）树脂一起使用，使蛋白质溶液和HCIC树脂柱接触，以使目标蛋白与其融合模拟物，洗涤树脂并通过pH梯度从树脂中洗脱目的蛋白质; 其中所述目标蛋白基本上不含其融合类似物。

9. 发明申请

US20080185333A1 SILANE-TREATED SILICA FILTER MEDIA 失效
标题翻译：硅烷处理二氧化硅过滤介质
公开(公告)号：US20080185333A1
公开(公告)日：2008-08-07
申请号：US12101743
申请日：2008-04-11
申请人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
发明人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
IPC分类号： B01D39/00
CPC分类号： B01J20/3259 , B01D15/361 , B01D15/3804 , B01J20/103 , B01J20/14 , B01J20/286 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3257 , B01J2220/4887 , Y10T436/25375
摘要： The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quarternary ammonium group or functional sulphonate group. The present invention also provides silane-treated silica filter media.
摘要翻译：本发明提供了从含有微粒和可溶性材料的样品中分离出一种或多种感兴趣组分的方法。该方法包括以下步骤：（a）通过其表面硅烷醇基团与一种或多种硅烷反应的二氧化硅过滤介质过滤样品，和（b）同时捕获微粒并将可溶组分结合到二氧化硅过滤介质上。随后从二氧化硅过滤介质中洗脱所结合的可溶性成分。在本发明的一个实施方案中，不希望的可溶物质被经处理的二氧化硅过滤介质捕获，并且所需的目的组分从流通中回收。在另一个实施方案中，感兴趣的不同成分从洗脱液和流通中回收。优选的经处理的二氧化硅过滤介质是具有功能季铵基或功能磺酸盐基团的硅烷处理的稻壳灰或硅藻土。本发明还提供硅烷处理的二氧化硅过滤介质。

10. 发明授权

US07264728B2 Method of separating components in a sample using silane-treated silica filter media 有权
标题翻译：使用硅烷处理的二氧化硅过滤介质分离样品中组分的方法
公开(公告)号：US07264728B2
公开(公告)日：2007-09-04
申请号：US10830935
申请日：2004-04-23
申请人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
发明人： Gary L. Gibson , Keith Hayes , Meng H. Heng , Csilla Kollar , Thomas H. Lane , Anthony Revis , Landon M. Steele
IPC分类号： B01D37/00
CPC分类号： B01J20/3259 , B01D15/361 , B01D15/3804 , B01J20/103 , B01J20/14 , B01J20/286 , B01J20/3204 , B01J20/3242 , B01J20/3244 , B01J20/3257 , B01J2220/4887 , Y10T436/25375
摘要： The present invention provides methods for separating one or more components of interest from a sample containing particulates and soluble materials. The method comprises the steps of: (a) filtering a sample through silica filter media whose surface silanol groups have reacted with one or more silanes, and (b) simultaneously capturing particulates and binding a soluble component to the silica filter media. The bound soluble component of interest is subsequently eluted from the silica filter media. In one embodiment of the invention, unwanted soluble materials are captured by the treated silica filter media and desired component of interest is recovered from the flow-through. In another embodiment of the invention, different components of interest are recovered from both the eluate and the flow-through. Preferred treated silica filter media are silane-treated rice hull ash or diatomaceous earth with functional quaternary ammonium group or functional sulphonate group. Particulates suitable for the present invention, for example, are microorganisms.
摘要翻译：本发明提供了从含有微粒和可溶性材料的样品中分离出一种或多种感兴趣组分的方法。该方法包括以下步骤：（a）通过其表面硅烷醇基团与一种或多种硅烷反应的二氧化硅过滤介质过滤样品，和（b）同时捕获微粒并将可溶组分结合到二氧化硅过滤介质上。随后从二氧化硅过滤介质中洗脱所结合的可溶性成分。在本发明的一个实施方案中，不希望的可溶物质被经处理的二氧化硅过滤介质捕获，并且所需的目的组分从流通中回收。在本发明的另一个实施方案中，感兴趣的不同成分从洗脱液和流通液中回收。优选的经处理的二氧化硅过滤介质是具有功能性季铵基团或官能磺酸盐基团的硅烷处理的稻壳灰或硅藻土。适用于本发明的微粒例如是微生物。

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