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    • 6. 发明申请
    • Extraction of nucleic acid
    • 提取核酸
    • US20050053941A1
    • 2005-03-10
    • US10496449
    • 2002-11-20
    • Matthew BakerMatthew TaylorShilpa Uppal
    • Matthew BakerMatthew TaylorShilpa Uppal
    • C12N15/09C12N15/10C12Q1/68C12Q1/6806C12N1/08
    • C12N15/1003C12Q1/6806
    • Methods of obtaining a sample of target nucleic acid from cells containing the target nucleic acid and genomic DNA or RNA are disclosed. In contrast to prior art protocols, this method does not require the cells containing the target nucleic acid to be lysed and instead is based on the observation when cells are suspended in an aqueous medium and the target nucleic acid are released into the medium through the cell walls. The invention therefore helps to avoid the use of cell lysis, heating, extremes of pH, water immiscible solvents, and electrical fields used in prior art nucleic acid extraction methods. The present invention is particularly applicable to the separation of non-genomic nucleic acid, such as cellular vector DNA or RNA, self-replicating satellite nucleic acids or plasmid DNA, from genomic nucleic acids, such as host cell chromosomes and ribosomal RNA.
    • 公开了从含有靶核酸和基因组DNA或RNA的细胞获得靶核酸样品的方法。 与现有技术方案相反,该方法不需要含有靶核酸的细胞被裂解,而是基于当细胞悬浮在水性介质中并且靶核酸通过细胞释放到培养基中时的观察 墙壁 因此,本发明有助于避免使用现有技术的核酸提取方法中使用的细胞裂解,加热,极端pH,水不混溶溶剂和电场。 本发明特别适用于从诸如宿主细胞染色体和核糖体RNA的基因组核酸分离非基因组核酸如细胞载体DNA或RNA,自复制卫星核酸或质粒DNA。
    • 7. 发明授权
    • Method and apparatus for detecting collisions on a network using multi-cycle waveform pulses
    • 用于使用多周期波形脉冲检测网络上的碰撞的方法和装置
    • US06735217B1
    • 2004-05-11
    • US09153635
    • 1998-09-15
    • Harold H. Webber, Jr.Jeremiah M. JeffressMatthew Taylor
    • Harold H. Webber, Jr.Jeremiah M. JeffressMatthew Taylor
    • H04L12413
    • H04L12/40032H04L12/413
    • A method and apparatus for detecting a collision between signal transmissions on a carrier medium, such as UTP wiring, are described. The method commences with the transmission of an identifier from a transmitter of a network device. The transmitted identifier has a predetermined duration and includes a pulse at a temporal location within the transmitted identifier specific thereto. A identifier is also received at a receiver of the network device. The received identifier also has a predetermined duration, and includes a pulse at a temporal location therein. A determination is then made as to whether the temporal location of the pulse within the received identifier corresponds to the temporal location of the pulse within the transmitted identifier. If not, a collision is detected on the network.
    • 描述了用于检测诸如UTP布线之类的载体介质上的信号传输之间的冲突的方法和装置。 该方法开始于从网络设备的发射机发送标识符。 所发送的标识符具有预定的持续时间,并且包括在所发送的标识符中的时间位置处的脉冲。 还在网络设备的接收机处接收标识符。 所接收的标识符也具有预定的持续时间,并且包括其中的时间位置处的脉冲。 然后确定接收的标识符内的脉冲的时间位置是否对应于所发送的标识符内的脉冲的时间位置。 如果没有,则在网络上检测到冲突。