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1. 发明申请

US20050239096A1 Detection of immobilized nucleic acid 有权
标题翻译：检测固定化核酸
公开(公告)号：US20050239096A1
公开(公告)日：2005-10-27
申请号：US10956868
申请日：2004-09-30
申请人： Matthew Beaudet , W. Cox , Stephen Yue
发明人： Matthew Beaudet , W. Cox , Stephen Yue
IPC分类号： C07D413/02 , C07D417/02 , C07D417/06 , C09B23/02 , C12Q1/68 , C07D43/02
CPC分类号： G01N21/64 , C07D417/06 , C09B23/02 , C09B23/04 , C12Q1/6816 , C12Q1/6825 , G01N1/30 , G01N2001/302 , Y10T436/143333 , C12Q2563/173 , C12Q2565/125
摘要： The present invention provides methods for determining the presence of immobilized nucleic acid employing unsymmetrical cyanine dyes that are derivatives of thiazole orange, a staining solution and select fluorogenic compounds that are characterized as being essentially non-genotoxic. The methods comprise immobilizing nucleic acid, single or double stranded DNA, RNA or a combination thereof, on a solid or semi solid support, contacting the immobilized nucleic acid with an unsymmetrical cyanine dye compound and then illuminating the immobilized nucleic acid with an appropriate wavelength whereby the presence of the nucleic acid is determined. The cyanine dye compounds are typically present in an aqueous staining solution comprising the dye compound and a tris acetate or tris borate buffer wherein the solution facilitates the contact of the dye compound and the immobilized nucleic acid. Typically the solid or semi-solid support is selected from the group consisting of a polymeric gel, a membrane, an array, a glass bead, a glass slide, and a polymeric microparticle. Preferably, the polymeric gel is agarose or polyacrylamide. The methods employing the non-genotoxic compounds represent an improvement over commonly used methods employing ethidium bromide wherein the present methods retain the advantages of ethidium bromide, ease of use and low cost, but without the disadvantageous, known mutagen requiring special handling and waste procedures.
摘要翻译：本发明提供了使用作为噻唑橙衍生物的不对称花青染料确定固定化核酸的存在的方法，染色溶液和选择性荧光化合物，其特征在于基本上是非遗传毒性的。所述方法包括在固体或半固体支持物上固定核酸，单链或双链DNA，RNA或其组合，使固定的核酸与不对称的花青染料化合物接触，然后用合适的波长照射固定的核酸，由此确定核酸的存在。花青染料化合物通常存在于包含染料化合物和三乙酸盐或三硼酸盐缓冲液的水性染色溶液中，其中溶液促进染料化合物和固定化核酸的接触。通常，固体或半固体支持物选自聚合物凝胶，膜，阵列，玻璃珠，载玻片和聚合物微粒。优选地，聚合物凝胶是琼脂糖或聚丙烯酰胺。使用非遗传毒性化合物的方法代表了使用溴化乙锭的常用方法的改进，其中本发明的方法保留了溴化乙锭的优点，易于使用和低成本，但没有不利的已知的需要特殊处理和废物方法的诱变剂。

2. 发明申请

US20060085280A1 Internet search engine with integrated e-commerce functionality 审中-公开
标题翻译：具有集成电子商务功能的互联网搜索引擎
公开(公告)号：US20060085280A1
公开(公告)日：2006-04-20
申请号：US11236219
申请日：2005-09-27
申请人： Dan Murnan , Daniel Porter , W. Cox
发明人： Dan Murnan , Daniel Porter , W. Cox
IPC分类号： G06Q30/00 , G06F17/30
CPC分类号： G06Q30/00 , G06F16/951 , G06Q30/0601 , G06Q40/04
摘要： Various embodiments of the present invention are directed to an Internet search engine with e-commerce functionality (“e-commerce search engine”). In one embodiment of the present invention, users can use the e-commerce search engine to locate retail items by typing in a key-word search. The search results displayed by the e-commerce search engine are vendor-website listings for vendors that sell retail items related to search terms. Some vendor-website listings may contain quick links through which a user can shop for, and purchase, retail items from the vendor through the e-commerce search engine, without being re-directed to the vendor's website. Transaction information is later relayed from the e-commerce search engine to the vendor. The vendor can then confirm the order and the shipping information with the user and complete the transaction.
摘要翻译：本发明的各种实施例涉及具有电子商务功能的因特网搜索引擎（“电子商务搜索引擎”）。在本发明的一个实施例中，用户可以使用电子商务搜索引擎通过键入关键字搜索来定位零售商品。电子商务搜索引擎显示的搜索结果是供应商网站列表，供应商出售与搜索字词相关的零售商品。一些供应商网站列表可能包含用户可以通过电子商务搜索引擎购买，以及从供应商购买零售商品的快速链接，而不会被重定向到供应商的网站。交易信息随后从电子商务搜索引擎转发给供应商。供应商可以确认订单和运送信息与用户完成交易。

3. 发明申请

US20050208598A1 Biotin recognition sensors and high-throughput assays 审中-公开
标题翻译：生物素识别传感器和高通量测定
公开(公告)号：US20050208598A1
公开(公告)日：2005-09-22
申请号：US11058345
申请日：2005-02-14
申请人： W. Cox , Iain Johnson
发明人： W. Cox , Iain Johnson
IPC分类号： C07D498/02 , C07F5/02 , C12M1/34 , C12Q1/68 , G01N33/53 , G01N33/542 , G01N33/543
CPC分类号： G01N33/5308 , G01N33/542
摘要： In one embodiment is provided a new class of biotin recognition sensors which comprise a biotin recognition compound (containing a biotin binding moiety), a fluorescent donor moiety, and an acceptor moiety. These compounds are useful for detecting biotin in a sample or on a carrier molecule. In addition to compounds, the invention also provides methods and kits for detecting the presence of biotin in a sample or on a carrier molecule.
摘要翻译：在一个实施方案中提供了一类新的生物素识别传感器，其包含生物素识别化合物（含有生物素结合部分），荧光供体部分和受体部分。这些化合物可用于检测样品或载体分子上的生物素。除了化合物之外，本发明还提供用于检测样品或载体分子上生物素的存在的方法和试剂盒。

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