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    • 5. 发明授权
    • Chromatographic two-layer particles
    • 色谱双层颗粒
    • US07208093B2
    • 2007-04-24
    • US10526088
    • 2003-08-18
    • Hans BergPhilippe BussonMats Carlsson
    • Hans BergPhilippe BussonMats Carlsson
    • B01D15/08
    • B01D15/363B01D15/1807B01J20/285B01J20/286B01J20/3285B01J20/3293B01J39/26B01J41/20
    • The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.
    • 本发明是一种制备色谱分离基质的方法,其中通过(a)在其表面上提供具有反应性基团的多孔聚合物颗粒,在两相体系中制备由具有不同性质的两层组成的多孔聚合物颗粒。 (b)用第一溶剂洗涤所述颗粒并排出颗粒以包围第一相; (c)通过加入基本不溶于第一溶剂的第二溶剂润湿颗粒外层; (d)通过添加在第一溶剂中基本上不反应的试剂使外层中的反应性基团反应; 和(e)色谱结合基团与内层中的反应性基团的偶合。 本发明还包括适合用作色谱分离基质的多孔聚合物颗粒和分离方法,其中使用根据本发明的基质。
    • 8. 发明授权
    • Manufacturing process for porous material
    • 多孔材料的制造工艺
    • US06984733B2
    • 2006-01-10
    • US10451194
    • 2001-12-19
    • Hans BergMats Carlsson
    • Hans BergMats Carlsson
    • C08B37/00
    • C08J9/28C08B37/0039C08J3/075C08J2201/0544C08J2305/12
    • A method for the manufacture of a solid porous separation material based on a polysaccharide, said method comprising the steps of: (a) providing an aqueous solution (I) of a polysaccharide, (b) solidifying the solution, preferably by transforming the solution to a gel, and (c) optionally crosslinking the polysaccharide, with the proviso that, if step (c) is present, steps (b) and (c) may be carried out simultaneously. The method is characterized in that the polysaccharide provided in step (a) is modified by being inter-molecularly crosslinked to an extent such that the viscosity of the solution (I) is at least 110%, preferably at least 200%, of the viscosity of an aqueous solution (II) of the corresponding polysaccharide which has not been intermolecularly crosslinked and which is present in the same concentration as the inter-molecularly crosslinked polysaccharide is in solution (I)
    • 一种制备基于多糖的固体多孔分离材料的方法,所述方法包括以下步骤:(a)提供多糖的水溶液(I),(b)固化溶液,优选通过将溶液转化为 凝胶,和(c)任选地交联多糖,条件是如果存在步骤(c),则可以同时进行步骤(b)和(c)。 该方法的特征在于,步骤(a)中提供的多糖通过分子间交联而被改性,使得溶液(I)的粘度至少为粘度的110%,优选至少为200% 未分子交联并且以与分子内交联的多糖相同的浓度存在的相应多糖的水溶液(II)在溶液(I)中,
    • 9. 发明申请
    • Chromatographic two-layer particles
    • 色谱双层颗粒
    • US20050242037A1
    • 2005-11-03
    • US10526088
    • 2003-08-18
    • Hans BergPhilippe BussonMats Carlsson
    • Hans BergPhilippe BussonMats Carlsson
    • B01J20/281B01D15/18B01J20/28B01J20/285B01J20/286G01N30/00G01N30/88B01D15/08
    • B01D15/363B01D15/1807B01J20/285B01J20/286B01J20/3285B01J20/3293B01J39/26B01J41/20
    • The present invention is a method of producing a chromatographic separation matrix, wherein porous polymeric particles comprised of two layers with different properties are prepared in a two-phase system by (a) providing porous polymeric particles with reactive groups on their surfaces; (b) washing said particles with a first solvent and draining the particles to enclose a first phase; (c) wetting the outer layer of the particles by adding a second solvent, which is essentially insoluble in the first solvent; (d) reacting the reactive groups in the outer layer by adding a reagent, which is essentially non-reactive in the first solvent; and (e) coupling of chromatographic binding groups to the reactive groups in the inner layer. The invention also encompasses a porous polymer particle suitable for use as a chromatographic separation matrix and a process of separation, wherein a matrix according to the invention is used.
    • 本发明是一种制备色谱分离基质的方法,其中通过(a)在其表面上提供具有反应性基团的多孔聚合物颗粒,在两相体系中制备由具有不同性质的两层组成的多孔聚合物颗粒。 (b)用第一溶剂洗涤所述颗粒并排出颗粒以包围第一相; (c)通过加入基本不溶于第一溶剂的第二溶剂润湿颗粒外层; (d)通过添加在第一溶剂中基本上不反应的试剂使外层中的反应性基团反应; 和(e)色谱结合基团与内层中的反应性基团的偶合。 本发明还包括适合用作色谱分离基质的多孔聚合物颗粒和分离方法,其中使用根据本发明的基质。
    • 10. 发明授权
    • Process for the production of polysaccharide beads
    • 生产多糖珠粒的方法
    • US06590096B1
    • 2003-07-08
    • US09806078
    • 2001-05-18
    • Hans BergMats Carlsson
    • Hans BergMats Carlsson
    • C07H504
    • C08J3/16C08J2305/00
    • The present invention relates to a method for producing porous polysaccharide beads, comprising: (a) mixing a water-based solution of a polysaccharide under stirring with an essentially water-immiscible first organic phase to form an emulsion which separates into two continuous phases; (b) adding a second organic phase comprising an emulsifier and an organic solvent to form a three-phase system; (c) allowing the three-phase system to emulsify; and (d) decreasing the temperature below the gelling point of the polysaccharide, thereby obtaining beads having two sets of pores, said method being characterised in that the emulsifier is a water-insoluble polymer capable of stabilizing said three-phase system.
    • 本发明涉及一种生产多孔多糖珠粒的方法,包括:(a)将搅拌下的多糖的水基溶液与基本上与水不混溶的第一有机相混合以形成分离成两个连续相的乳液; (b)加入包含乳化剂和有机溶剂的第二有机相以形成三相体系; (c)允许三相体系乳化; 和(d)降低低于多糖胶凝点的温度,从而获得具有两组孔的珠粒,所述方法的特征在于乳化剂是能够稳定所述三相体系的水不溶性聚合物。