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1. 发明授权

US08178322B2 Method for producing an L-amino acid or a nucleic acid 有权
标题翻译： L-氨基酸或核酸的制造方法
公开(公告)号：US08178322B2
公开(公告)日：2012-05-15
申请号：US12541204
申请日：2009-08-14
申请人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
发明人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
IPC分类号： C12P19/40 , C12P19/30 , C12P19/34 , C12P13/04 , C12P13/22 , C12P13/12 , C12P13/08 , C12P13/06
CPC分类号： C12P13/04 , C12P13/08 , C12P13/14 , C12P13/22 , C12P19/32 , C12P19/40
摘要： A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
摘要翻译：描述了通过在含有搅拌叶轮的发酵罐中培养具有在液体培养基中产生L-氨基酸或核酸的能力的微生物来生产L-氨基酸或核酸的方法，并且任选地加入晶种在培养基中产生并积累L-氨基酸或核酸的晶体并从培养物中收集L-氨基酸或核酸的晶体的培养基中。搅拌叶轮的功率密度在晶体沉淀或加入晶种后控制在2.4 kW / m3或更低。

2. 发明申请

US20100062493A1 METHOD FOR PRODUCING AN L-AMINO ACID OR A NUCLEIC ACID 有权
标题翻译：生产L-氨基酸或核酸的方法
公开(公告)号：US20100062493A1
公开(公告)日：2010-03-11
申请号：US12541204
申请日：2009-08-14
申请人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
发明人： Masayuki Araki , Yusuke Takahashi , Akihiro Watanabe , Fumito Ohnishi , Takahiro Asano , Kazuya Kondo , Wataru Hibino , Shintaro Iwatani , Satoshi Okutani
IPC分类号： C12P19/34 , C12P13/04 , C12P13/22 , C12P13/06 , C12P13/08 , C12P13/14 , C12P13/12
CPC分类号： C12P13/04 , C12P13/08 , C12P13/14 , C12P13/22 , C12P19/32 , C12P19/40
摘要： A method is described for producing an L-amino acid or a nucleic acid by culturing a microorganism having an ability to produce the L-amino acid or nucleic acid in a liquid medium in a fermentation tank containing a stirring impeller, and optionally adding seed crystals to the medium as required to produce and accumulate crystals of the L-amino acid or nucleic acid in the medium, and collecting crystals of the L-amino acid or nucleic acid from the culture. The power density of the stirring impeller is controlled to be 2.4 kW/m3 or lower after either precipitation of the crystals or addition of the seed crystals.
摘要翻译：描述了通过在含有搅拌叶轮的发酵罐中培养具有在液体培养基中产生L-氨基酸或核酸的能力的微生物来生产L-氨基酸或核酸的方法，并且任选地加入晶种在培养基中产生并积累L-氨基酸或核酸的晶体并从培养物中收集L-氨基酸或核酸的晶体的培养基中。搅拌叶轮的功率密度在晶体沉淀或加入晶种后控制在2.4 kW / m3或更低。

3. 发明授权

US06852516B2 Method for producing L-glutamic acid by fermentation 失效
标题翻译：通过发酵生产L-谷氨酸的方法
公开(公告)号：US06852516B2
公开(公告)日：2005-02-08
申请号：US10307320
申请日：2002-12-02
申请人： Wataru Hibino , Yasuhiko Yoshihara , Masakazu Sugimoto , Harufumi Miwa
发明人： Wataru Hibino , Yasuhiko Yoshihara , Masakazu Sugimoto , Harufumi Miwa
IPC分类号： C12N15/09 , C07C51/00 , C12N1/21 , C12N15/00 , C12P13/14 , C12R1/13 , C12R1/15 , C07H21/04 , C12N1/20 , C12N9/00
CPC分类号： C12P13/14
摘要： A coryneform bacterium which has enhanced intracellular pyruvate carboxylase activity obtained by increasing copy number of a gene encoding the intracellular pyruvate carboxylase, or by enhancing function of a expression regulatory sequence for the gene, and has L-glutamic acid-producing ability is cultured in a medium so that L-glutamic acid should be produced and accumulated in the culture, and L-glutamic acid is collected from the culture.
摘要翻译：通过增加编码细胞内丙酮酸羧化酶的基因的拷贝数或通过增强基因的表达调节序列的功能而获得的具有增强的细胞内丙酮酸羧化酶活性并具有L-谷氨酸生产能力的棒状细菌被培养在培养基中生成L-谷氨酸并在培养物中积累，从培养物中收集L-谷氨酸。

4. 发明授权

US06258573B1 Method of producing L-serine by fermentation 失效
标题翻译：通过发酵生产L-丝氨酸的方法
公开(公告)号：US06258573B1
公开(公告)日：2001-07-10
申请号：US09222786
申请日：1998-12-30
申请人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
发明人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
IPC分类号： C12P1306
CPC分类号： C12N9/0006 , C12P13/06 , C12R1/13 , C12R1/15 , C12R1/19
摘要： Disclosed is a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity. Also, disclosed are D-3-phosphoglycerate dehydrogenase derived from a coryneform bacterium, in which feedback inhibition by L-serine is desensitized; the D-3-phosphoglycerate dehydrogenase, which is obtainable from a coryneform bacterium having resistance to azaserine or &bgr;-(2-thienyl)-DL-alanine and having L-serine productivity; the D-3-phosphoglycerate dehydrogenase having an amino acid sequence depicted in SEQ ID NO: 12 in Sequence Listing or the sequence including substitution, addition or deletion of one or more amino acids, wherein an amino acid residue corresponding to the 325th glutamic acid residue of the amino acid sequence in the SEQ ID NO: 12 is replaced with an amino acid other than glutamic acid; the D-3-phosphoglycerate dehydrogenase that has an amino acid sequence depicted in SEQ ID NO: 11 in Sequence Listing; a DNA coding for the D-3-phosphoglycerate dehydrogenase described above; the DNA that has a base sequence depicted in SEQ ID NO: 13 in Sequence Listing; a coryneform bacterium which harbors a recombinant DNA containing the DNA; and a method of producing L-serine, comprising the steps of cultivating the bacterium described above in a medium to allow accumulation of L-serine in the medium, and collecting the L-serine from the medium.
摘要翻译：公开了具有对丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有L-丝氨酸生产力的抗性的棒状细菌。此外，公开了衍生自棒状细菌的D-3-磷酸甘油酸脱氢酶，其中L-丝氨酸的反馈抑制被脱敏; D-3-磷酸甘油酸脱氢酶，其可从具有对β-丝氨酸或β-（2-噻吩基）-DL-丙氨酸具有抗丝氨酸生产力的棒状细菌获得; 具有序列表中SEQ ID NO：12所示氨基酸序列的D-3-磷酸甘油酸脱氢酶或包含一个或多个氨基酸的取代，添加或缺失的序列，其中对应于第325位谷氨酸残基的氨基酸残基 SEQ ID NO：12中的氨基酸序列被除了谷氨酸以外的氨基酸替换; 具有序列表中SEQ ID NO：11所示的氨基酸序列的D-3-磷酸甘油酸脱氢酶; 编码上述D-3-磷酸甘油酸脱氢酶的DNA; 具有序列表中SEQ ID NO：13所示碱基序列的DNA; 含有含DNA的重组DNA的棒状细菌; 以及生产L-丝氨酸的方法，包括以下步骤：在培养基中培养上述细菌以允许L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

5. 发明授权

US6037154A Method of producing L-serine by fermentation 有权
标题翻译：通过发酵生产L-丝氨酸的方法
公开(公告)号：US6037154A
公开(公告)日：2000-03-14
申请号：US222817
申请日：1998-12-30
申请人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
发明人： Mikiko Suga , Masakazu Sugimoto , Tsuyoshi Osumi , Tsuyoshi Nakamatsu , Wataru Hibino , Mika Ito
IPC分类号： C12N15/09 , C12N1/20 , C12N1/21 , C12N9/04 , C12N15/53 , C12P13/06 , C12R1/13 , C12N1/12 , C12N9/14
CPC分类号： C12N9/0006 , C12P13/06 , C12R1/13 , C12R1/15 , C12R1/19
摘要： L-serine is produced by cultivating in a medium a coryneform bacterium having L-serine productivity in which an activity of at least one of phosphoserine phosphatase and phosphoserine transaminase is enhanced, preferably, further having introduced therein a gene coding for D-3-phosophoglycerate dehydrogenase in which feedback inhibition by L-serine is desensitized, allowing L-serine to accumulate in the medium, and collecting the L-serine from the medium.
摘要翻译：通过在培养基中培养具有L-丝氨酸生产力的棒状细菌，其中磷酸丝氨酸磷酸酶和磷酸丝氨酸转氨酶中的至少一种的活性增强，优选进一步在其中引入编码D-3-磷酸甘油酸的基因而产生L-丝氨酸其中L-丝氨酸的反馈抑制脱敏，使L-丝氨酸积累在培养基中，并从培养基中收集L-丝氨酸。

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