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    • 8. 发明申请
    • METHOD OF ESTIMATING SECONDARY STRUCTURE IN RNA AND PROGRAM AND APPARATUS THEREFOR
    • 估计RNA及其程序中的二级结构的方法及其设备
    • US20100063745A1
    • 2010-03-11
    • US12518854
    • 2007-12-13
    • Shingo Nakamura
    • Shingo Nakamura
    • G06F19/00G06F17/18
    • G06Q50/22G16B15/00G16B45/00
    • Assuming frame F having specific short length L2 on a transcript, a structure in a small-section is sequentially analyzed while shifting the frame F finely in a step-like manner by constant value t, and first probability for a specific secondary structure in a specific position in each small-section is determined. Then, at least one specific position proved in each small-section is arranged in corresponding position on the original transcript, and the magnitude of degree of overlapping between the specific positions is determined as second probability. By focusing on these two probabilities, it is possible to predict whether an intended secondary structure is actually present in RNA such as mRNA with higher reliability.
    • 假定帧F在转录本上具有特定的短长度L2,则以阶梯状方式将帧F精细地移动恒定值t,依次分析小部分中的结构,并且将特定二级结构的特定的第一概率 确定每个小节中的位置。 然后,在每个小部分中证明的至少一个具体位置被布置在原始誊本上的对应位置,并且确定特定位置之间的重叠程度的大小作为第二概率。 通过关注这两个概率,可以预测目标二级结构实际上是否存在于RNA中,如具有较高可靠性的mRNA。
    • 9. 发明申请
    • Method Of Screening Compound Regulating The Translation Of Specific mRNA
    • 筛选化合物调节特异性mRNA翻译的方法
    • US20090019042A1
    • 2009-01-15
    • US11791161
    • 2005-11-18
    • Shingo NakamuraHiroyuki Ueno
    • Shingo NakamuraHiroyuki Ueno
    • G06F7/20G06F17/30
    • C12N15/111C12N15/113C12N15/67C12N2310/11C12N2320/11G16B15/00G16B20/00G16B30/00G16B35/00G16C20/60
    • The present invention provides a screening method for a compound capable of preventing/treating a particular disease, which comprises (1) a step for selecting one or more mRNAs whose regulation of translation can result in the prevention/treatment of the disease, (2) a step for searching for mRNA(s) having a sequence, in the molecule, capable of assuming a local secondary structure capable of regulating the translation of the mRNA(s) from among the mRNAs selected in (1) above, (3) a step for selecting a particular sequence from among sequences capable of assuming a local secondary structure in the particular mRNA extracted in (2) above, (4) a step for confirming that a partial structure capable of interacting with the compound in the particular sequence selected in (3) above is not present in the region involved in the regulation of the translation of other mRNA, or even if present, is incapable of regulating the translation, and (5) a step for bringing an RNA strand comprising a particular sequence confirmed to be not present in the region involved in the regulation of the translation of other mRNA in (4) above and a test compound into contact with each other, and measuring changes in the stability of the secondary structure of the RNA strand.
    • 本发明提供了能够预防/治疗特定疾病的化合物的筛选方法,其包括(1)选择一种或多种mRNA的步骤,所述mRNA的调节可导致疾病的预防/治疗,(2) 在分子中搜索具有能够在上述(1)中选择的mRNA中能够调节mRNA的翻译的局部二级结构的分子中具有序列的mRNA的步骤,(3)a 从在上述(2)中提取的特定mRNA中能够呈现局部二级结构的序列中选择特定序列的步骤,(4)确认能够与特定序列中化合物相互作用的部分结构的步骤 (3)不存在于涉及其他mRNA的翻译调节的区域中,或者即使存在也不能调节翻译,以及(5)使包含一个或多个mRNA的RNA链的步骤 确认在涉及上述(4)中其他mRNA的翻译调节区域和测试化合物相互接触并测定RNA链二级结构稳定性变化的区域中不存在细目序列。