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1. 发明申请

US20090019605A1 PLANT HAVING REDUCED LIGNIN AND CELLULOSE CONTENTS WITHOUT REDUCING GLUCAN CONTENT, METHOD OF PRODUCING THE SAME AND UTILIZATION THEREOF 有权
标题翻译：具有减少葡萄糖含量的减少木质素和纤维素含量的植物，其生产方法及其利用
公开(公告)号：US20090019605A1
公开(公告)日：2009-01-15
申请号：US12280722
申请日：2007-02-27
申请人： Masaru Takagi , Nobutaka Mitsuda , Akira Iwase , Keiichiro Hiratsu
发明人： Masaru Takagi , Nobutaka Mitsuda , Akira Iwase , Keiichiro Hiratsu
IPC分类号： C12N15/11 , A01H5/00 , C12N15/82
CPC分类号： C12N15/8246 , C12N15/8216 , C12N15/8255 , G01N33/5097 , G01N33/56961
摘要： By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.
摘要翻译：通过抑制促进与木质素和纤维素的量相关的基因的转录的转录因子的功能，产生其中木素和纤维素的量减少而不减少葡聚糖的量的植物。在该植物中，获得的细胞壁组分中的葡聚糖处于非常容易进行糖化的状态。此外，在这种植物中，荚的自然开裂被抑制。抑制转录因子的方法包括将转录因子基因和能够将转录因子转录成转录抑制子的功能性肽的多核苷酸之间的嵌合基因引入植物细胞中，使嵌合蛋白质在植物细胞中产生转录因子与功能性肽融合的转录因子，以及抑制转录因子表达的方法，例如敲除方法或RNAi方法。因此，提供了在不降低葡聚糖的量的情况下还原木质素和纤维素的量的植物。

2. 发明授权

US08183432B2 Plant having reduced lignin and cellulose contents without reducing glucan content, method of producing the same and utilization thereof 有权
标题翻译：具有降低木素和纤维素含量而不降低葡聚糖含量的植物，其生产方法及其利用
公开(公告)号：US08183432B2
公开(公告)日：2012-05-22
申请号：US12280722
申请日：2007-02-27
申请人： Masaru Takagi , Nobutaka Mitsuda , Akira Iwase , Keiichiro Hiratsu
发明人： Masaru Takagi , Nobutaka Mitsuda , Akira Iwase , Keiichiro Hiratsu
IPC分类号： C12N15/82 , C12N15/10 , C12N15/00 , A01H5/00
CPC分类号： C12N15/8246 , C12N15/8216 , C12N15/8255 , G01N33/5097 , G01N33/56961
摘要： By inhibiting the function of a transcription factor that promotes transcription of a gene associated with the amounts of lignin and cellulose, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is produced. In this plant, glucan in the obtained cell wall components is in the state of highly easily undergoing saccharification. In this plant, moreover, natural dehiscence of pods is suppressed. A method of inhibiting the transcription factor includes a method in which a chimeric gene between a transcription factor gene and a polynucleotide that encodes a functional peptide capable of converting the transcription factor into a transcription repressor is introduced into a plant cell so that a chimeric protein in which the transcription factor is fused with the functional peptide is produced in a plant cell, and a method of inhibiting the expression of the transcription factor, such as knockout method or RNAi method. Thus, a plant in which the amounts of lignin and cellulose are reduced without reducing the amount of glucan is provided.
摘要翻译：通过抑制促进与木质素和纤维素的量相关的基因的转录的转录因子的功能，产生其中木素和纤维素的量减少而不减少葡聚糖的量的植物。在该植物中，获得的细胞壁组分中的葡聚糖处于非常容易进行糖化的状态。此外，在这种植物中，荚的自然开裂被抑制。抑制转录因子的方法包括将转录因子基因和能够将转录因子转录成转录抑制子的功能性肽的多核苷酸之间的嵌合基因引入植物细胞中，使嵌合蛋白质在植物细胞中产生转录因子与功能性肽融合的转录因子，以及抑制转录因子表达的方法，例如敲除方法或RNAi方法。因此，提供了在不降低葡聚糖的量的情况下还原木质素和纤维素的量的植物。

3. 发明申请

US20110099664A1 Producing process of sterile plants, plants obtained by the process, and use of the plants 审中-公开
标题翻译：无菌植物的生产过程，通过该过程获得的植物和植物的使用
公开(公告)号：US20110099664A1
公开(公告)日：2011-04-28
申请号：US10574470
申请日：2005-01-07
申请人： Masaru Takagi , Keiichiro Hiratsu , Nobutaka Mitsuda
发明人： Masaru Takagi , Keiichiro Hiratsu , Nobutaka Mitsuda
IPC分类号： A01H5/00 , C12N15/82 , C12N5/04 , A01H5/10 , C12N15/63
CPC分类号： C12N15/8289 , C12N15/829
摘要： Transcription of a gene associated with formation of floral organs is suppressed to produce a sterile plant. A plant cell is transfected with a chimeric gene that includes (i) a coding gene of a transcription factor that promotes expression of a gene associated with formation of floral organs, and (ii) a polynucleotide that encodes a functional peptide that converts an arbitrary transcription factor into a transcription repressor, and a chimeric protein in which the transcription factor is fused with the functional peptide is expressed in the plant cell. The expression of the gene associated with formation of floral organs is dominantly suppressed by the chimeric protein, and as a result a male sterile plant is produced that cannot properly form pollen. The chimeric protein also suppresses expression of a gene associated with dehiscence of anther, and as a result a plant is produced in which dehiscence of anther is suppressed. Further, the chimeric protein suppresses expression of target genes of a transcription factor associated with formation of stamen and pistil, and as a result a double flowered plant is produced.
摘要翻译：抑制与花器官形成相关的基因的转录以产生无菌植物。用嵌合基因转染植物细胞，该嵌合基因包括（i）促进与花器官形成有关的基因表达的转录因子的编码基因，和（ii）编码功能性肽的多核苷酸，其转换任意转录因子转录入抑制子，并且其中转录因子与功能性肽融合的嵌合蛋白在植物细胞中表达。与花器官形成相关的基因的表达主要被嵌合蛋白抑制，结果产生不能正确形成花粉的雄性不育植物。嵌合蛋白还抑制与花药开裂有关的基因的表达，结果产生了抑制花药开裂的植物。此外，嵌合蛋白质抑制与雄蕊和雌蕊形成相关的转录因子的靶基因的表达，结果产生双花植物。

4. 发明申请

US20120331591A1 METHOD OF PRODUCING CYCLAMEN WITH MULTI-PETALED FLOWERS 有权
标题翻译：用多花瓣生产环孢菌素的方法
公开(公告)号：US20120331591A1
公开(公告)日：2012-12-27
申请号：US13516505
申请日：2010-12-16
申请人： Teruhiko Terakawa , Tomomichi Yamamura , Masao Sugiyama , Masaru Takagi , Nobutaka Mitsuda
发明人： Teruhiko Terakawa , Tomomichi Yamamura , Masao Sugiyama , Masaru Takagi , Nobutaka Mitsuda
IPC分类号： A01N43/50 , A01P21/00 , A01H5/00
CPC分类号： C12N15/827 , C12N15/8216
摘要： A method of producing a multi-petaled cyclamen plant having an increased number of petals, including at least inhibiting the function of a transcription factor involved in morphogenesis of a floral organ of cyclamen.
摘要翻译：一种生产花瓣数目增多的多花瓣仙客花植物的方法，包括至少抑制参与仙客来花器官形态发生的转录因子的功能。

5. 发明授权

US08847011B2 Genes that increase plant oil and method for using the same 有权
标题翻译：增加植物油的基因及其使用方法
公开(公告)号：US08847011B2
公开(公告)日：2014-09-30
申请号：US12746577
申请日：2008-12-05
申请人： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
发明人： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
IPC分类号： C12N15/82 , C12P21/02 , C12N15/09 , C07H21/04
CPC分类号： C12N15/8247 , C07K7/06 , C07K14/415 , C12N15/8216 , C12N15/8217 , C12N15/8245 , C12N15/8261 , Y02A40/146
摘要： This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
摘要翻译：本发明旨在用于搜索具有增加单个植物的重量，增加每个植物的给定组织的重量或提高每个植物的给定物质的生产力的新功能的转录因子并改善植物中的这种特性。单个植物的重量增加，每个植物的给定组织的重量增加，每个植物的给定物质的生产力得到改善，或者通过表达增加每个给定组织的给定物质的含量已被修饰以抑制转录加速活性的转录因子。

6. 发明授权

US09376688B2 Method of producing cyclamen with multi-petaled flowers 有权
标题翻译：用多花瓣生产仙客来的方法
公开(公告)号：US09376688B2
公开(公告)日：2016-06-28
申请号：US13516505
申请日：2010-12-16
申请人： Teruhiko Terakawa , Tomomichi Yamamura , Masao Sugiyama , Masaru Takagi , Nobutaka Mitsuda
发明人： Teruhiko Terakawa , Tomomichi Yamamura , Masao Sugiyama , Masaru Takagi , Nobutaka Mitsuda
IPC分类号： C12N15/82 , C12N15/29
CPC分类号： C12N15/827 , C12N15/8216
摘要： A method of producing a multi-petaled cyclamen plant having an increased number of petals, including at least inhibiting the function of a transcription factor involved in morphogenesis of a floral organ of cyclamen.
摘要翻译：一种生产花瓣数目增多的多花瓣仙客花植物的方法，包括至少抑制参与仙客来花器官形态发生的转录因子的功能。

7. 发明申请

US20110010804A1 GENES THAT INCREASE PLANT OIL AND METHOD FOR USING THE SAME 有权
标题翻译：增加植物油的基因和使用它们的方法
公开(公告)号：US20110010804A1
公开(公告)日：2011-01-13
申请号：US12746577
申请日：2008-12-05
申请人： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
发明人： Hiroshi Chatani , Chikara Ohto , Yukio Okamura , Norihiro Mitsukawa , Nobuhiko Muramoto , Masaru Takagi , Nobutaka Mitsuda , Tomotsugu Koyama , Kyoko Matsui
IPC分类号： C12N15/63 , A01H5/00 , C07K19/00 , C07H21/04 , C12N5/00 , C10L1/14 , A23D9/02
CPC分类号： C12N15/8247 , C07K7/06 , C07K14/415 , C12N15/8216 , C12N15/8217 , C12N15/8245 , C12N15/8261 , Y02A40/146
摘要： This invention is intended to be used to search for a transcription factor having novel functions of increasing the weight of an individual plant, increasing the weight of a given tissue per individual plant, or improving the productivity of a given substance per individual plant and to improve such properties in the plant. The weight of an individual plant is increased, the weight of a given tissue per individual plant is increased, the productivity of a given substance per individual plant is improved, or the content of a given substance per given tissue of a plant is increased via expression of a transcription factor that has been modified to suppress transcription accelerating activity.
摘要翻译：本发明旨在用于搜索具有增加单个植物的重量，增加每个植物的给定组织的重量或提高每个植物的给定物质的生产力的新功能的转录因子并改善植物中的这种特性。单个植物的重量增加，每个植物的给定组织的重量增加，每个植物的给定物质的生产力得到改善，或者通过表达增加每个给定组织的给定物质的含量已被修饰以抑制转录加速活性的转录因子。

8. 发明授权

US09303265B2 Gene for increasing plant weight and method for using the same 有权
标题翻译：增加植物体重的基因及其使用方法
公开(公告)号：US09303265B2
公开(公告)日：2016-04-05
申请号：US13376138
申请日：2010-06-04
申请人： Satoshi Kondo , Chikara Ohto , Masaru Takagi , Kyoko Matsui , Tomotsugu Koyama , Nobutaka Mitsuda , Nobuhiko Muramoto , Norihiro Mitsukawa , Tomoko Tanaka
发明人： Satoshi Kondo , Chikara Ohto , Masaru Takagi , Kyoko Matsui , Tomotsugu Koyama , Nobutaka Mitsuda , Nobuhiko Muramoto , Norihiro Mitsukawa , Tomoko Tanaka
IPC分类号： C12N15/82 , C07K14/415
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/82 , C12N15/8261
摘要： A gene having novel functions is searched for, by which plant weight (that is, biomass level) can be increased and by which substance productivity can be increased or decreased. A chimeric protein is expressed in which a transcriptional factor comprising the amino acid sequence shown in SEQ ID NO: 2, 4, or 6 is fused to a functional peptide that converts an arbitrary transcriptional factor into a transcriptional repression factor.
摘要翻译：搜索具有新功能的基因，通过该基因可以增加植物体重（即生物量），并且可以提高或降低物质生产力。表达嵌合蛋白，其中将包含SEQ ID NO：2,4或6所示氨基酸序列的转录因子与将任意转录因子转化为转录抑制因子的功能肽融合。

9. 发明申请

US20120159673A1 GENE FOR INCREASING PLANT WEIGHT AND METHOD FOR USING THE SAME 有权
标题翻译：用于增加植物重量的基因和使用它们的方法
公开(公告)号：US20120159673A1
公开(公告)日：2012-06-21
申请号：US13376138
申请日：2010-06-04
申请人： Satoshi Kondo , Chikara Ohto , Masaru Takagi , Kyoko Matsui , Tomotsugu Koyama , Nobutaka Mitsuda , Nobuhiko Muramoto , Norihiro Mitsukawa , Tomoko Tanaka
发明人： Satoshi Kondo , Chikara Ohto , Masaru Takagi , Kyoko Matsui , Tomotsugu Koyama , Nobutaka Mitsuda , Nobuhiko Muramoto , Norihiro Mitsukawa , Tomoko Tanaka
IPC分类号： A01H5/00 , C12N5/10 , C12N15/82 , C07K14/415 , C12N15/29
CPC分类号： C12N15/8247 , C07K14/415 , C12N15/82 , C12N15/8261
摘要： A gene having novel functions is searched for, by which plant weight (that is, biomass level) can be increased and by which substance productivity can be increased or decreased. A chimeric protein is expressed in which a transcriptional factor comprising the amino acid sequence shown in SEQ ID NO: 2, 4, or 6 is fused to a functional peptide that converts an arbitrary transcriptional factor into a transcriptional repression factor.
摘要翻译：搜索具有新功能的基因，通过该基因可以增加植物体重（即生物量），并且可以提高或降低物质生产力。表达嵌合蛋白，其中将包含SEQ ID NO：2,4或6所示氨基酸序列的转录因子与将任意转录因子转化为转录抑制因子的功能肽融合。

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