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    • 2. 发明授权
    • Method for preparation of a recombinant DNA phage
    • 重组DNA噬菌体的制备方法
    • US4348478A
    • 1982-09-07
    • US87167
    • 1979-10-22
    • Eiichi NakanoTsutomu MasudaNarimasa SaitoDanji Fukushima
    • Eiichi NakanoTsutomu MasudaNarimasa SaitoDanji Fukushima
    • C12N15/09C12N15/00C12P19/34C12R1/91
    • C12N15/00
    • A method for the preparation of a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a temperate phage DNA having an endonuclease-sensitive region not in the DNA segment participating in the replication of phage DNA and the integration of phage DNA into a host chromosome but at least in the DNA segment carrying genetic information for the coat protein production and another DNA carrying intended genetic information, (2) adding DNA-ligase to the mixture of both cleft DNA's, and (3) recovering from the mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segments carrying genetic information for the coat protein production with a DNA fragment carrying the intended information.
    • 一种制备新的重组DNA的方法,其包括(1)用核酸内切酶切割温和的噬菌体DNA,所述温带噬菌体DNA具有参与噬菌体DNA复制的DNA片段中不具有内切核酸酶敏感区域,并将噬菌体DNA整合到 宿主染色体,但至少在DNA片段中携带外壳蛋白生产的遗传信息和携带预期遗传信息的另一个DNA,(2)向裂解DNA的混合物中加入DNA连接酶,和(3)从混合物中回收噬菌体 通过用携带预期信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段,缺失其外壳蛋白产生能力的DNA。
    • 4. 发明授权
    • Method for preparing a recombinant DNA phage
    • 制备重组DNA噬菌体的方法
    • US4348477A
    • 1982-09-07
    • US87166
    • 1979-10-22
    • Eiichi NakanoTsutomu MasudaNarimasa SaitoDanji Fukushima
    • Eiichi NakanoTsutomu MasudaNarimasa SaitoDanji Fukushima
    • C12N15/09C12N15/00C12P19/34C12R1/91
    • C12N15/00
    • A method for preparing a novel recombinant DNA, which comprises (1) cleaving with an endonuclease a phage DNA having an endonuclease-sensitive region not in the DNA segment participating in temperate phage DNA replication and integration of DNA into a host chromosome but in other DNA segments, a temperate phage DNA having an endonuclease-sensitive region in the DNA segment carrying genetic information for the production of coat protein, and a DNA carrying the intended genetic information, (2) mixing together all fragments produced by said cleaving, (3) adding DNA ligase to the mixture, and (4) recovering from the resulting mixture a phage DNA having its coat protein producing ability deleted by the replacement of the DNA segment carrying genetic information for coat protein production with a DNA fragment carrying the intended genetic information.
    • 一种制备新型重组DNA的方法,其包括(1)用核酸内切酶切割具有不在DNA片段中的内切核酸酶敏感区域的噬菌体DNA,其参与温和噬菌体DNA复制并将DNA整合到宿主染色体中,但在其他DNA (2)将由所述切割产生的所有片段混合在一起,(3)将所述切割产生的所有片段混合在一起,其中所述DNA片段具有用于产生外壳蛋白的遗传信息的DNA段中具有内切核酸酶敏感区的温带噬菌体DNA, 向混合物中加入DNA连接酶,(4)从所得混合物中回收具有外壳蛋白产生能力的噬菌体DNA,通过用携带预期遗传信息的DNA片段替代携带用于外壳蛋白生产的遗传信息的DNA片段而缺失。