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1. 发明授权

US4352881A Method of measuring creatine kinase activity 失效
标题翻译：测定肌酸激酶活性的方法
公开(公告)号：US4352881A
公开(公告)日：1982-10-05
申请号：US302868
申请日：1981-09-16
申请人： Masanobu Inagawa , Toshiyuki Sai , Seiichi Kawarabuki , Naoto Miwa
发明人： Masanobu Inagawa , Toshiyuki Sai , Seiichi Kawarabuki , Naoto Miwa
IPC分类号： G01N33/70 , C12Q1/32 , C12Q1/48 , C12Q1/50 , G01N33/72
CPC分类号： C12Q1/32 , C12Q1/50
摘要： A method and composition for use therein for measuring the activity of creatine kinase (hereinafter referred to as "CK") having good storage stability is disclosed. More particularly, a composition for measurement of CK activity which comprises as main components phosphoglycerate kinase (hereinafter referred to a "PGK") which catalyzes the following reaction 2 and glyceraldehyde-3-phosphate dehydrogenase (hereinafter referred to as "GAPDH") which catalyzes the following reaction 3. Reaction 1 is catalyzed by the creatine kinase.Reaction 1Creatinephosphate+Adenosine-5'-diphosphate (hereinafter referred to as "ADP").revreaction.Creatine+Adenosine-5'-triphosphate (hereinafter referred to as "ATP")Reaction 2ATP+3-Phosphoglycerate.revreaction.ADP+1,3-DiphosphoglycerateReaction 31,3-Diphosphoglycerate+Reduce .beta.-nicotinamideadenine dinucleotide (hereinafter referred to as "NADH").revreaction.Glyceraldehyde-3-phosphate+.beta.-nicotinamideadenine dinucleotide (hereinafter referred to as "NAD")+Inorganic phoshate
摘要翻译：公开了一种用于测量具有良好储存稳定性的肌酸激酶（以下称为“CK”）活性的方法和组合物。更具体地，涉及用于测定CK活性的组合物，其包含催化以下反应的磷酸甘油酸激酶（以下称为“PGK”）和催化下列反应的甘油醛-3-磷酸脱氢酶（以下称为“GAPDH”）的主要成分以下反应3.反应1由肌酸激酶催化。反应1磷酸肌酸+腺苷-5'-二磷酸（以下称为“ADP”） - >肌酸+腺苷-5'-三磷酸（以下称为“ATP”）反应2 ATP + 3-磷酸甘油 - -ADP + 1,3-二磷酸甘油酸反应3 1,3-二磷酸甘油酸+减少β-烟酰胺腺苷二核苷酸（以下简称“NADH”） - > - - 甘油醛-3-磷酸+β-烟酰胺腺嘌呤二核苷酸

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