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1. 发明申请

US20100151531A1 METHOD FOR AMPLIFICATION OF DNA FROM BLOOD SAMPLE AND DNA AMPLIFICATION KIT THEREFOR 有权
标题翻译：用于从血液样品和DNA扩增试剂盒中放大DNA的方法
公开(公告)号：US20100151531A1
公开(公告)日：2010-06-17
申请号：US12637239
申请日：2009-12-14
申请人： Masamitsu SHIKATA
发明人： Masamitsu SHIKATA
IPC分类号： C12P19/34 , C12N9/96
CPC分类号： C12Q1/6846 , C12Q2537/119 , C12Q2527/119
摘要： A DNA amplification method including: subjecting a blood sample having DNA to be amplified, to a pretreatment using an alkaline aqueous solution under ordinary temperature, so as to extract double-stranded DNA from the blood sample and dissociate the double-stranded DNA into a single-stranded DNA to obtain a blood-derived sample including the single-stranded DNA; preparing an isothermal amplification reaction solution comprising a mixture of the blood-derived sample, a primer, dNTP, a strand-displacing DNA polymerase, a magnesium salt and a buffer, to establish an isothermal amplification reaction system meeting optimum conditions for the strand-displacing DNA polymerase; and amplifying DNA in the isothermal amplification reaction system using the single-stranded DNA as a template.
摘要翻译：一种DNA扩增方法，其特征在于，将通过DNA扩增的血液样品在常温下使用碱性水溶液进行预处理，从血液样品中提取双链DNA，将双链DNA解离为单一DNA 品种DNA以获得包含单链DNA的血液衍生物样品; 制备包含血液衍生的样品，引物，dNTP，链置换DNA聚合酶，镁盐和缓冲液的混合物的等温扩增反应溶液，以建立满足链取代的最佳条件的等温扩增反应系统 DNA聚合酶; 并使用单链DNA作为模板在等温扩增反应体系中扩增DNA。

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