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1. 发明授权

US08465919B2 Method of detecting methylated cytosine 有权
标题翻译：检测甲基化胞嘧啶的方法
公开(公告)号：US08465919B2
公开(公告)日：2013-06-18
申请号：US12674648
申请日：2008-08-20
申请人： Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
发明人： Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
IPC分类号： C12Q1/68 , C07H21/00 , C07H21/02 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q2537/164 , C12Q2525/119 , C12Q2523/115
摘要： The method for detecting a methylated cytosine of the present invention comprises the steps of: hybridizing a sample DNA with an oligonucleotide which can hybridize with a region of the sample DNA containing a cytosine suspected of being methylated and has an a basic site at the position complementary to the cytosine; reacting the hybridized sample DNA obtained in the previous step with an oxidizing agent to oxidize the cytosine when it is methylated; and detecting the oxidized methylated cytosine.
摘要翻译：检测本发明的甲基化胞嘧啶的方法包括以下步骤：将样品DNA与能够与疑似被甲基化的胞嘧啶的样品DNA的区域杂交的寡核苷酸杂交，并且在互补位置具有基本位点到胞嘧啶; 使上一步获得的杂交样品DNA与氧化剂反应，以在甲基化时氧化胞嘧啶; 并检测氧化的甲基化胞嘧啶。

2. 发明申请

US20100240042A1 METHOD OF DETECTING METHYLATED CYTOSINE 有权
标题翻译：检测甲基化胞嘧啶的方法
公开(公告)号：US20100240042A1
公开(公告)日：2010-09-23
申请号：US12674648
申请日：2008-08-20
申请人： Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
发明人： Masahiro Kajita , Noriko Oka , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
IPC分类号： C12Q1/68 , C07H21/04 , C12M1/34
CPC分类号： C12Q1/6827 , C12Q2537/164 , C12Q2525/119 , C12Q2523/115
摘要： The method for detecting a methylated cytosine of the present invention comprises the steps of:hybridizing a sample DNA with an oligonucleotide which can hybridize with a region of the sample DNA containing a cytosine suspected of being methylated and has an a basic site at the position complementary to the cytosine;reacting the hybridized sample DNA obtained in the previous step with an oxidizing agent to oxidize the cytosine when it is methylated; anddetecting the oxidized methylated cytosine.
摘要翻译：检测本发明的甲基化胞嘧啶的方法包括以下步骤：将样品DNA与能够与疑似被甲基化的胞嘧啶的样品DNA的区域杂交的寡核苷酸杂交，并且在互补位置具有基本位点到胞嘧啶; 使上一步获得的杂交样品DNA与氧化剂反应，以在甲基化时氧化胞嘧啶; 并检测氧化的甲基化胞嘧啶。

3. 发明授权

US08906623B2 Kit and method for determining whether or not unmethylated cytosine conversion treatment is properly carried out and method for analyzing methylated DNA using the same 有权
标题翻译：用于确定是否正确进行非甲基化胞嘧啶转化处理的试剂盒和方法以及使用其分析甲基化DNA的方法
公开(公告)号：US08906623B2
公开(公告)日：2014-12-09
申请号：US12935846
申请日：2009-03-16
申请人： Kaya Tai , Ayako Sakai , Noriaki Yamamoto , Noriko Oka , Masahiro Kajita
发明人： Kaya Tai , Ayako Sakai , Noriaki Yamamoto , Noriko Oka , Masahiro Kajita
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q2523/125
摘要： Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.
摘要翻译：通过使用包含多个引物的第一引物组来确定未甲基化胞嘧啶转化处理的成功或失败，所述多个引物与包含未经甲基化胞嘧啶转化处理的生物DNA的核苷酸序列中不含胞嘧啶的核苷酸序列的核酸杂交，第二引物组，其包含与包含核苷酸序列的核酸杂交的多个引物，所述核酸序列在生物DNA的核苷酸序列中将含有胞嘧啶并且不含有CpG位点的核苷酸序列中的胞嘧啶转化为除胞嘧啶以外的碱基。

4. 发明申请

US20110027799A1 KIT AND METHOD FOR DETERMINING WHETHER OR NOT UNMETHYLATED CYTOSINE CONVERSION TREATMENT IS PROPERLY CARRIED OUT AND METHOD FOR ANALYZING METHYLATED DNA USING THE SAME 有权
标题翻译：用于确定是否合成非甲基化胞嘧啶转化酶处理的试剂盒和方法以及使用其分析甲基化DNA的方法
公开(公告)号：US20110027799A1
公开(公告)日：2011-02-03
申请号：US12935846
申请日：2009-03-16
申请人： Kaya Tai , Ayako Sakai , Noriaki Yamamoto , Noriko Oka , Masahiro Kajita
发明人： Kaya Tai , Ayako Sakai , Noriaki Yamamoto , Noriko Oka , Masahiro Kajita
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6827 , C12Q2523/125
摘要： Success or failure of unmethylated cytosine conversion treatment is determined by using a first primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence not containing cytosine in the nucleotide sequence of biological DNA that is subject to an unmethylated cytosine conversion treatment and a second primer set comprising plural primers that hybridize with a nucleic acid comprising a nucleotide sequence in which cytosine in a nucleotide sequence containing cytosine and not containing a CpG site is converted into a base other than cytosine, in the nucleotide sequence of biological DNA.
摘要翻译：通过使用包含多个引物的第一引物组来确定未甲基化胞嘧啶转化处理的成功或失败，所述多个引物与包含未经甲基化胞嘧啶转化处理的生物DNA的核苷酸序列中不含胞嘧啶的核苷酸序列的核酸杂交，第二引物组，其包含与包含核苷酸序列的核酸杂交的多个引物，所述核酸序列在生物DNA的核苷酸序列中将含有胞嘧啶并且不含有CpG位点的核苷酸序列中的胞嘧啶转化为除胞嘧啶以外的碱基。

5. 发明申请

US20080096213A1 Method for confirming conversion treatment and nucleic acid molecule used therefor 审中-公开
标题翻译：用于确认转化处理的方法和用于其的核酸分子
公开(公告)号：US20080096213A1
公开(公告)日：2008-04-24
申请号：US11902254
申请日：2007-09-20
申请人： Masahiro Kajita , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
发明人： Masahiro Kajita , Noriaki Yamamoto , Ayako Sakai , Hideki Ishihara
IPC分类号： C12Q1/68 , C07H21/00
CPC分类号： C12Q1/6827 , C12Q2565/1015 , C12Q2545/114 , C12Q2545/101
摘要： There is provided a method for confirming conversion treatment of non-methylated cytosine, including a step of mixing a DNA taken from a living body, with a nucleic acid for accuracy control having a sequence A which is not contained in a genome of the living body and contains cytosine, a step of converting non-methylated cytosine in the mixture into a base other than cytosine, a step of detecting the sequence A and/or a sequence A′ which has a sequence obtained by converting cytosine in the sequence A into the base other than cytosine, and a step of determining whether the conversion step has been properly performed or not based on the result of the detection step.
摘要翻译：提供了一种确认非甲基化胞嘧啶的转化处理的方法，包括将从活体取得的DNA混合的步骤与用于精确控制的核酸的步骤，所述序列A不包含在生物体的基因组中并含有胞嘧啶，将混合物中的非甲基化胞嘧啶转化为除胞嘧啶以外的碱基的步骤，检测序列A和/或A序列的步骤，其具有通过将序列A中的胞嘧啶转化为碱基以外的碱基，以及基于检测步骤的结果确定转化步骤是否已经被适当地进行的步骤。

6. 发明申请

US20090123923A1 Method for obtaining information regarding quantity of DNA after non-methylated cytosine converting treatment in analysis of DNA methylation 审中-公开
标题翻译：在DNA甲基化分析中获得非甲基化胞嘧啶转化处理后的DNA量的方法
公开(公告)号：US20090123923A1
公开(公告)日：2009-05-14
申请号：US11987461
申请日：2007-11-30
申请人： Noriaki Yamamoto , Masahiro Kajita , Takayuki Takahata , Ayako Sakai , Hideki Ishihara
发明人： Noriaki Yamamoto , Masahiro Kajita , Takayuki Takahata , Ayako Sakai , Hideki Ishihara
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6851 , C12Q2523/125 , C12Q2531/113
摘要： The present invention provides an easy and accurate method for obtaining information regarding a quantity of DNA included in a sample used for analyzing a methylation of a target DNA, comprising steps of: treating a biological sample containing DNA including the target DNA with a non-methylated cytosine converting agent for converting non-methylated cytosine of the DNA into another base; and obtaining information regarding a quantity of DNA included in the sample treated in the treating step by using an oligonucleotide complementary to a cytosine-free region of the target DNA.
摘要翻译：本发明提供了一种用于获得关于用于分析靶DNA的甲基化的样品中包含的DNA量的信息的简单且准确的方法，包括以下步骤：用含有非甲基化的DNA包含靶DNA的生物样品用于将DNA的非甲基化胞嘧啶转化成另一种碱基的胞嘧啶转化剂; 并且通过使用与靶DNA的无胞嘧啶区域互补的寡核苷酸获得关于处理步骤中处理的样品中包含的DNA的量的信息。

7. 发明申请

US20090111090A1 METHOD FOR DETECTING INTEGRATED HPV DNA 有权
标题翻译：检测整合型HPV DNA的方法
公开(公告)号：US20090111090A1
公开(公告)日：2009-04-30
申请号：US12262508
申请日：2008-10-31
申请人： Noriko OKA , Masahiro Kajita , Hideki Ishihara
发明人： Noriko OKA , Masahiro Kajita , Hideki Ishihara
IPC分类号： C12Q1/70
CPC分类号： C12Q1/6886
摘要： A method for detecting integrated HPV DNA is described herein. This method comprises obtaining first and second samples, obtaining first and second information, and detecting, based on the first and second information, the HPV DNA integrated into the genome of a cell derived from a subject. The second sample comprises DNA derived from the cell, which is treated with an enzyme having exonuclease activity. The first information is related to the amount of HPV DNA in the first sample, and the second information is related to the amount of HPV DNA in the second sample.
摘要翻译：本文描述了用于检测整合的HPV DNA的方法。该方法包括获得第一和第二样本，获得第一和第二信息，并且基于第一和第二信息检测整合到从受试者得到的细胞的基因组中的HPV DNA。第二个样品包含源自细胞的DNA，其用具有核酸外切酶活性的酶处理。第一个信息与第一个样本中HPV DNA的数量相关，第二个信息与第二个样品中HPV DNA的数量有关。

8. 发明申请

US20090246784A1 METHOD OF ANALYZING METHYLATED DNA 有权
标题翻译：分析甲基化DNA的方法
公开(公告)号：US20090246784A1
公开(公告)日：2009-10-01
申请号：US12403167
申请日：2009-03-12
申请人： Ayako SAKAI , Masahiro Kajita , Hideki Ishihara
发明人： Ayako SAKAI , Masahiro Kajita , Hideki Ishihara
IPC分类号： C12Q1/68 , C07H21/04
CPC分类号： C12Q1/6827 , C12Q2521/331 , C12Q2531/113
摘要： The present invention provides a method of analyzing methylated DNA, comprising steps of: (A) treating a DNA-containing sample with a restriction enzyme to obtain a sample containing a DNA fragment; (B) concentrating methylated DNA contained in the sample obtained in step (A) to obtain a methylated DNA concentrate; (C) subjecting the methylated DNA concentrate obtained in step (B) and a primer set to nucleic acid amplification reaction, wherein the primer set performs the nucleic acid amplification reaction in step (C) by using a template DNA which does not have a CpG site; (D) detecting an amplification product obtained in step (C); (E) judging whether the methylated DNA concentrate obtained in step (B) is appropriate as a sample for detection of methylated DNA, on the basis of the detection result of the amplification product in step (D); and(F) analyzing the methylated DNA contained in the methylated DNA concentrate.
摘要翻译：本发明提供一种分析甲基化DNA的方法，包括以下步骤：（A）用限制酶处理含有DNA的样品，得到含有DNA片段的样品; （B）浓缩步骤（A）中获得的样品中所含的甲基化DNA以获得甲基化DNA浓缩物; （C）使步骤（B）中获得的甲基化DNA浓缩物和引物组进行核酸扩增反应，其中引物组通过使用不具有CpG的模板DNA进行步骤（C）中的核酸扩增反应现场; （D）检测步骤（C）中获得的扩增产物; （E）基于步骤（D）中的扩增产物的检测结果判断步骤（B）中获得的甲基化DNA浓缩物是否适合作为检测甲基化DNA的样品; 和（F）分析甲基化DNA浓缩物中所含的甲基化DNA。

9. 发明授权

US08257916B2 Method for detecting integrated HPV DNA 有权
标题翻译：检测HPV整合的方法
公开(公告)号：US08257916B2
公开(公告)日：2012-09-04
申请号：US12262508
申请日：2008-10-31
申请人： Noriko Oka , Masahiro Kajita , Hideki Ishihara
发明人： Noriko Oka , Masahiro Kajita , Hideki Ishihara
IPC分类号： C12Q1/70 , C12P19/34
CPC分类号： C12Q1/6886
摘要： A method for detecting integrated HPV DNA is described herein. This method comprises obtaining first and second samples, obtaining first and second information, and detecting, based on the first and second information, the HPV DNA integrated into the genome of a cell derived from a subject. The second sample comprises DNA derived from the cell, which is treated with an enzyme having exonuclease activity. The first information is related to the amount of HPV DNA in the first sample, and the second information is related to the amount of HPV DNA in the second sample.
摘要翻译：本文描述了用于检测整合的HPV DNA的方法。该方法包括获得第一和第二样本，获得第一和第二信息，并且基于第一和第二信息检测整合到从受试者得到的细胞的基因组中的HPV DNA。第二个样品包含源自细胞的DNA，其用具有核酸外切酶活性的酶处理。第一个信息与第一个样本中HPV DNA的数量相关，第二个信息与第二个样品中HPV DNA的数量有关。

10. 发明授权

US08163490B2 Method of analyzing methylated DNA 有权
标题翻译：分析甲基化DNA的方法
公开(公告)号：US08163490B2
公开(公告)日：2012-04-24
申请号：US12403167
申请日：2009-03-12
申请人： Ayako Sakai , Masahiro Kajita , Hideki Ishihara
发明人： Ayako Sakai , Masahiro Kajita , Hideki Ishihara
IPC分类号： C12Q1/68 , C12P19/34
CPC分类号： C12Q1/6827 , C12Q2521/331 , C12Q2531/113
摘要： The present invention provides a method of analyzing methylated DNA, comprising steps of: (A) treating a DNA-containing sample with a restriction enzyme to obtain a sample containing a DNA fragment; (B) concentrating methylated DNA contained in the sample obtained in step (A) to obtain a methylated DNA concentrate; (C) subjecting the methylated DNA concentrate obtained in step (B) and a primer set to nucleic acid amplification reaction, wherein the primer set performs the nucleic acid amplification reaction in step (C) by using a template DNA which does not have a CpG site; (D) detecting an amplification product obtained in step (C); (E) judging whether the methylated DNA concentrate obtained in step (B) is appropriate as a sample for detection of methylated DNA, on the basis of the detection result of the amplification product in step (D); and (F) analyzing the methylated DNA contained in the methylated DNA concentrate.
摘要翻译：本发明提供一种分析甲基化DNA的方法，包括以下步骤：（A）用限制酶处理含有DNA的样品，得到含有DNA片段的样品; （B）浓缩步骤（A）中获得的样品中所含的甲基化DNA以获得甲基化DNA浓缩物; （C）使步骤（B）中获得的甲基化DNA浓缩物和引物组进行核酸扩增反应，其中引物组通过使用不具有CpG的模板DNA进行步骤（C）中的核酸扩增反应现场; （D）检测步骤（C）中获得的扩增产物; （E）基于步骤（D）中的扩增产物的检测结果判断步骤（B）中获得的甲基化DNA浓缩物是否适合作为检测甲基化DNA的样品; 和（F）分析甲基化DNA浓缩物中所含的甲基化DNA。

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