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    • 3. 发明授权
    • Dual-state nutritional medium for the transport and maintenance of cells
    • 用于运输和维持细胞的双态营养培养基
    • US5593880A
    • 1997-01-14
    • US194575
    • 1994-02-10
    • William J. NorthamJohn D. BarryRichard L. Heberling
    • William J. NorthamJohn D. BarryRichard L. Heberling
    • C12M1/02C12N5/02A01N1/02C12M1/16C12M1/18
    • A01N1/0231A01N1/0273A01N1/0284
    • A nutritional medium for enabling the growth, maintenance and transport of cells in a container of virtually any size or shape, including a substantially flat, multi-well plastic plate. The nutritional medium is capable of assuming a substantially liquid state above a first temperature and a substantially solid state below a second temperature. Both the first and second temperatures are generally compatible with the survival of the cells in the container. The cells are covered with the medium while the medium is in a substantially liquid state, and the temperature of the medium is then reduced to a temperature at which the medium assumes a substantially solid state. The medium is maintained in a substantially solid state while the cells are transported. Upon arrival at the end user, the temperature of the medium is increased to a temperature at which the medium assumes a substantially liquid state. Following a change to fresh maintenance medium, the cells in the container are ready for immediate use.
    • 一种营养培养基,用于使细胞生长,维持和运输在几乎任何尺寸或形状的容器中,包括基本平坦的多孔塑料板。 营养培养基能够在低于第二温度的第一温度和基本上固体状态下呈现基本上液体的状态。 第一和第二温度都通常与容器中的细胞的存活相容。 当培养基处于基本上液体状态时,细胞被培养基覆盖,然后将培养基的温度降低到培养基呈现基本上固态的温度。 当传送细胞时,介质保持基本上固态。 在到达最终用户时,介质的温度升高到介质呈现基本上液态的温度。 在更换新的维护介质后,容器中的电池可以立即使用。