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1. 发明授权

US08557576B2 Mineralized three-dimensional bone constructs 有权
标题翻译：矿化三维骨构造
公开(公告)号：US08557576B2
公开(公告)日：2013-10-15
申请号：US12781482
申请日：2010-05-17
申请人： Mark S. F. Clarke , Alamelu Sundaresan , Neal R. Pellis
发明人： Mark S. F. Clarke , Alamelu Sundaresan , Neal R. Pellis
IPC分类号： C12N5/07 , A61F2/28
CPC分类号： A61L27/3847 , A61L27/3821 , A61L27/3891 , A61L27/3895 , A61L2430/02 , C12N5/00 , C12N5/0654 , C12N2500/42 , C12N2501/22 , C12N2501/39 , C12N2501/60 , C12N2502/11 , C12N2525/00
摘要： The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.
摘要翻译：本公开提供离体衍生的矿化三维骨构建体。通过在随机重力载体条件下培养成骨细胞和破骨细胞前体获得骨构建体。优选地，使用低剪切应力旋转生物反应器（例如高纵横比容器（HARV）培养系统）获得随机重力矢量条件。本公开的骨构建体在骨形成和骨骼功能，药物发现和整形外科的生理学研究中具有实用性。

2. 发明授权

US08076136B2 Mineralized three-dimensional bone constructs 有权
标题翻译：矿化三维骨构造
公开(公告)号：US08076136B2
公开(公告)日：2011-12-13
申请号：US11693662
申请日：2007-03-29
申请人： Mark S. F. Clarke , Alamelu Sundaresan , Neal R. Pellis
发明人： Mark S. F. Clarke , Alamelu Sundaresan , Neal R. Pellis
IPC分类号： C12N5/07
CPC分类号： A61L27/3847 , A61L27/3821 , A61L27/3891 , A61L27/3895 , A61L2430/02 , C12N5/00 , C12N5/0654 , C12N2500/42 , C12N2501/22 , C12N2501/39 , C12N2501/60 , C12N2502/11 , C12N2525/00
摘要： The present disclosure provides ex vivo-derived mineralized three-dimensional bone constructs. The bone constructs are obtained by culturing osteoblasts and osteoclast precursors under randomized gravity vector conditions. Preferably, the randomized gravity vector conditions are obtained using a low shear stress rotating bioreactor, such as a High Aspect Ratio Vessel (HARV) culture system. The bone constructs of the disclosure have utility in physiological studies of bone formation and bone function, in drug discovery, and in orthopedics.
摘要翻译：本公开提供离体衍生的矿化三维骨构建体。通过在随机重力载体条件下培养成骨细胞和破骨细胞前体获得骨构建体。优选地，使用低剪切应力旋转生物反应器（例如高纵横比容器（HARV）培养系统）获得随机重力矢量条件。本公开的骨构建体在骨形成和骨骼功能，药物发现和整形外科的生理学研究中具有实用性。

3. 发明授权

US08506982B2 Development of a human colloidal bone graft material 失效
标题翻译：开发人体胶体骨移植材料
公开(公告)号：US08506982B2
公开(公告)日：2013-08-13
申请号：US12742806
申请日：2008-11-14
申请人： Alamelu Sundaresan , Mark S. F. Clarke , Mark Brinker
发明人： Alamelu Sundaresan , Mark S. F. Clarke , Mark Brinker
IPC分类号： A61F2/00
CPC分类号： A61L27/365 , A61L27/3608 , A61L27/38 , A61L2430/02
摘要： The present disclosure provides colloidal bone graft compositions comprising a plurality of mineralized three-dimensional bone constructs, a polymerizable biocompatible matrix, an osteoblast differentiation agent, and at least one osteoblast mineralization agent. The colloidal bone graft compositions of the disclosure are used to perform bone grafts in human or animal patients.
摘要翻译：本公开提供了包含多个矿化的三维骨构建体，可聚合生物相容性基质，成骨细胞分化剂和至少一种成骨细胞矿化剂的胶体骨移植组合物。本公开的胶体骨移植组合物用于在人或动物患者中进行骨移植。

4. 发明授权

US07763229B2 Isolation and purification of single walled carbon nanotube structures 有权
标题翻译：单壁碳纳米管结构的分离和纯化
公开(公告)号：US07763229B2
公开(公告)日：2010-07-27
申请号：US11656690
申请日：2007-01-23
申请人： Pavel Nikolaev , Sivaram Arepalli , Mark S. F. Clarke , Daniel L. Feeback
发明人： Pavel Nikolaev , Sivaram Arepalli , Mark S. F. Clarke , Daniel L. Feeback
IPC分类号： D01F9/12 , C09C1/56
CPC分类号： B82Y30/00 , D01F9/127 , Y10S977/745 , Y10S977/845 , Y10S977/847
摘要： Disclosed are methods for isolating and purifying single wall carbon nanotubes from contaminant matrix material, methods for forming arrays of substantially aligned nanotubes, and products and apparatus comprising a plurality of nanotube structures.
摘要翻译：公开了用于从污染物基体材料中分离和纯化单壁碳纳米管的方法，用于形成基本排列的纳米管阵列的方法，以及包括多个纳米管结构的产物和设备。

5. 发明授权

US6008009A Centrifuge-operated specimen staining method and apparatus 失效
标题翻译：离心机样品染色方法及装置
公开(公告)号：US6008009A
公开(公告)日：1999-12-28
申请号：US174380
申请日：1998-10-15
申请人： Mark S. F. Clarke , Daniel L. Feeback
发明人： Mark S. F. Clarke , Daniel L. Feeback
IPC分类号： G01N1/30 , G01N1/31 , G01N35/00 , C12Q1/00 , C12Q1/08
CPC分类号： G01N1/30 , G01N1/31 , G01N2035/00336 , Y10S435/96
摘要： A method of staining preselected, mounted specimens of either biological or nonbiological material enclosed within a staining chamber where the liquid staining reagents are applied and removed from the staining chamber using hypergravity as the propelling force. In the preferred embodiment, a spacecraft-operated centrifuge and method of diagnosing biological specimens while in orbit, characterized by hermetically sealing a shell assembly. The assembly contains slide stain apparatus with computer control therefor, the operative effect of which is to overcome microgravity, for example on board an International Space Station.
摘要翻译：将预先选定的，安装在染色室内的生物或非生物材料的样品的染色方法，其中使用超重力作为推进力施加液体染色试剂并从染色室除去。在优选实施例中，航天器操作的离心机和在轨道上诊断生物样本的方法，其特征在于密封壳体组件。该组件包含具有计算机控制的滑动污渍装置，其操作效果是克服微重力，例如在国际空间站上。

6. 发明授权

US06221666B1 Method and apparatus for cytoplasmic loading using an impact-mediated procedure 失效
标题翻译：使用冲击介导程序进行细胞质负载的方法和装置
公开(公告)号：US06221666B1
公开(公告)日：2001-04-24
申请号：US09094548
申请日：1998-06-12
申请人： Mark S. F. Clarke , Rob G. Lucas-Dean , Daniel L. Feeback , Charles R. Vanderburg , Michael M. Withey
发明人： Mark S. F. Clarke , Rob G. Lucas-Dean , Daniel L. Feeback , Charles R. Vanderburg , Michael M. Withey
IPC分类号： C12N1587
CPC分类号： C12N1/066 , C12M35/04
摘要： The present invention is directed to the method and apparatus for the cytoplasmic loading of macromolecules into living cells by an impact-mediated procedure that impacts the cells with a predetermined number of solid particles in a blast of propellant gas. More specifically, the present invention is directed to an impact-mediated procedure that is altered by gravitational conditions and is preferably carried out under hypergravity conditions. Further, the present invention is directed to an IML method and apparatus for consistently and reproducibly loading macromolecules into the cytoplasm of living cells via membrane wounding at significantly higher efficiencies than can be accomplished using existing methodologies. The IML procedure directs a blast of propellant gas through a rupturable membrane on which solid particles are supported in order to achieve insertion of a predetermined number of particles into the propellant blast. Another preferred embodiment is a particle containment shell that includes a particle support membrane that ruptures at a predetermined propellant blast pressure and has a predetermined number of particles resting on the surface of the support membrane.
摘要翻译：本发明涉及通过冲击介导的方法将大分子细胞质负载到活细胞中的方法和装置，所述方法和装置在推进剂气体的鼓风中以预定数量的固体颗粒撞击细胞。更具体地，本发明涉及通过重力条件改变的冲击介导过程，并且优选在超重力条件下进行。此外，本发明涉及用于将大分子一贯地和可重复地加载到通过膜损伤的活细胞的细胞质以显着更高的效率比使用现有方法可以实现。 IML程序通过支撑固体颗粒的可破裂的膜引导爆炸的推进剂气体，以便将预定数量的颗粒插入到推进剂爆炸物中。另一个优选的实施方案是颗粒容纳壳，其包括以预定的推进剂鼓风压力破裂并且具有预定数量的颗粒搁置在支撑膜的表面上的颗粒支撑膜。

7. 发明授权

US07731929B2 Spatial localization of dispersed single walled carbon nanotubes into useful structures 有权
标题翻译：分散的单壁碳纳米管的空间定位成有用的结构
公开(公告)号：US07731929B2
公开(公告)日：2010-06-08
申请号：US10943018
申请日：2004-09-17
申请人： Mark S. F. Clarke
发明人： Mark S. F. Clarke
IPC分类号： D01F9/12
CPC分类号： B82Y30/00 , D01F11/14 , Y10S977/745 , Y10S977/845 , Y10T428/2918
摘要： Methods of aligning single walled carbon nanotube structures into selected orientations for a variety of different applications are achieved by initially dispersing the nanotube structures in aqueous solutions utilizing a suitable dispersal agent. The dispersal agent coats each individual nanotube structure in solution. The dispersal agent may be substituted with a suitable functional group that reacts with a corresponding binding site. Dispersed nanotube structures coated with substituted dispersal agents are exposed to a selected array of binding sites such that the nanotubes align with the binding sites due to the binding of the substituted functional groups with such binding sites. Alternatively, crystalline nanotube material is formed upon deposition of dispersed nanotube structures within solution into channels disposed on the surface of the substrate. Combining dispersal agent chemical modification techniques with deposition of the nanotubes into substrate channels is also utilized to produce useful structures.
摘要翻译：通过使用合适的分散剂将纳米管结构初步分散在水溶液中来实现将单壁碳纳米管结构对准用于各种不同应用的选定取向的方法。分散剂在溶液中涂覆每个单独的纳米管结构。分散剂可以用与相应结合位点反应的合适官能团取代。涂覆有取代的分散剂的分散的纳米管结构暴露于所选择的结合位点阵列，使得由于取代的官能团与这种结合位点的结合，纳米管与结合位点对准。或者，在分散的纳米管结构在溶液中沉积到设置在基底表面上的通道中之后形成结晶纳米管材料。将分散剂化学修饰技术与纳米管沉积到基底通道中相结合也被用于产生有用的结构。

8. 发明授权

US07166266B2 Isolation and purification of single walled carbon nanotube structures 有权
标题翻译：单壁碳纳米管结构的分离和纯化
公开(公告)号：US07166266B2
公开(公告)日：2007-01-23
申请号：US10191631
申请日：2002-07-09
申请人： Pavel Nikolaev , Sivaram Arepalli , Mark S. F. Clarke , Daniel L. Feeback
发明人： Pavel Nikolaev , Sivaram Arepalli , Mark S. F. Clarke , Daniel L. Feeback
IPC分类号： C01B31/02
CPC分类号： B82Y30/00 , D01F9/127 , Y10S977/745 , Y10S977/845 , Y10S977/847
摘要： Disclosed are methods for isolating and purifying single wall carbon nanotubes from contaminant matrix material, methods for forming arrays of substantially aligned nanotubes, and products and apparatus comprising a plurality of nanotube structures.
摘要翻译：公开了用于从污染物基体材料中分离和纯化单壁碳纳米管的方法，用于形成基本排列的纳米管阵列的方法，以及包括多个纳米管结构的产物和设备。

9. 发明授权

US06743632B2 Directional acceleration vector-driven displacement of fluids (DAVD-DOF) 失效
标题翻译：流体的定向加速度矢量驱动位移（DAVD-DOF）
公开(公告)号：US06743632B2
公开(公告)日：2004-06-01
申请号：US09805057
申请日：2001-03-14
申请人： Mark S. F. Clarke , Daniel L. Feeback
发明人： Mark S. F. Clarke , Daniel L. Feeback
IPC分类号： G01N3500
CPC分类号： B01L3/502707 , B01L3/502723 , B01L3/50273 , B01L9/527 , B01L2200/0689 , B01L2300/0803 , B01L2400/0409 , B01L2400/0633 , G01N1/31 , G01N21/07 , G01N2035/00178 , G01N2035/00336 , Y10T436/11 , Y10T436/111666 , Y10T436/112499
摘要： Centrifugal analyzer and method for staining biological or non-biological samples in microgravity, wherein the method utilizes an increase in weight of a fluid sample as a function of g-load, to overcome cohesive and frictional forces from preventing its movement in a preselected direction. Apparatus is characterized by plural specimen reservoirs and channels in a slide, each channel being of differing cross-section, wherein respective samples are selectively dispensed, from the reservoirs in response to an imposed g-factor, precedent to sample staining. Within the method, one thus employs microscope slides which define channels, each being of a differing cross-section dimension relative to others. In combination therewith, centrifugal slide mounting apparatus controllably imposes g-vectors of differing magnitudes within a defined structure of the centrifuge such as a chip array.
摘要翻译：用于在微重力中染色生物或非生物样品的离心分析仪和方法，其中所述方法利用作为g-载荷的函数的流体样品的重量增加，以克服粘性和摩擦力以防止其沿预选方向移动。装置的特征在于载玻片中的多个样品储存器和通道，每个通道具有不同的横截面，其中响应于施加的g因子，样品染色的先例，从储存器中选择性地分配相应的样品。在该方法中，因此使用限定通道的显微镜载玻片，每个通道相对于其它通道具有不同的横截面尺寸。与此同时，离心式滑动安装装置可控制地将不同大小的g向量施加在离心机的限定结构内，例如芯片阵列。

10. 发明授权

US06896864B2 Spatial localization of dispersed single walled carbon nanotubes into useful structures 有权
标题翻译：分散的单壁碳纳米管的空间定位成有用的结构
公开(公告)号：US06896864B2
公开(公告)日：2005-05-24
申请号：US09983830
申请日：2001-10-26
申请人： Mark S. F. Clarke
发明人： Mark S. F. Clarke
IPC分类号： B82B3/00 , C01B31/02 , D01F11/14 , D01F9/12
CPC分类号： B82Y30/00 , D01F11/14 , Y10S977/745 , Y10S977/845 , Y10T428/2918
摘要： Methods of aligning single walled carbon nanotube structures into selected orientations for a variety of different applications are achieved by initially dispersing the nanotube structures in aqueous solutions utilizing a suitable dispersal agent. The dispersal agent coats each individual nanotube structure in solution. The dispersal agent may be substituted with a suitable functional group that reacts with a corresponding binding site. Dispersed nanotube structures coated with substituted dispersal agents are exposed to a selected array of binding sites such that the nanotubes align with the binding sites due to the binding of the substituted functional groups with such binding sites. Alternatively, crystalline nanotube material is formed upon deposition of dispersed nanotube structures within solution into channels disposed on the surface of the substrate. Combining dispersal agent chemical modification techniques with deposition of the nanotubes into substrate channels is also utilized to produce useful structures.
摘要翻译：通过使用合适的分散剂将纳米管结构初步分散在水溶液中来实现将单壁碳纳米管结构对准用于各种不同应用的选定取向的方法。分散剂在溶液中涂覆每个单独的纳米管结构。分散剂可以用与相应结合位点反应的合适的官能团代替。涂覆有取代的分散剂的分散的纳米管结构暴露于所选择的结合位点阵列，使得由于取代的官能团与这种结合位点的结合，纳米管与结合位点对准。或者，在分散的纳米管结构在溶液中沉积到设置在基底表面上的通道中之后形成结晶纳米管材料。将分散剂化学修饰技术与纳米管沉积到基底通道中相结合也被用于产生有用的结构。

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