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2. 发明申请

US20120005773A1 TRANSGENIC PLANTS WITH ENHANCED AGRONOMIC TRAITS 审中-公开
标题翻译：具有增强农业条件的转基因植物
公开(公告)号：US20120005773A1
公开(公告)日：2012-01-05
申请号：US12998243
申请日：2009-09-30
申请人： Eric D. Aasen , Mark Scott Abad , Edwards Allen , Thandoni Rao Ambika , Veena S. Anil , Alice Clara Augustine , Stephanie L. Back , Pranesh Badami , Amarjit Basra , Kraig L. Brustad , Molian Deng , Todd Dezwaan , Charles Dietrich , Stephen M. Duff , Karen K. Gabbert , Steve He , Bill L. Hendrix , Hongwu Jia , Vidya Kammaradi Ramanath Bhat , Hongwu Jia , Vidya Kammaradi Ramanath Bhat , Balasulojini Karunanandaa , Kevin R. Kosola , Kempagangaiah Krishnamurthy , Ganesh Kumar , Saritha V. Kuriakose , Shantala Lakkanna , Mark E. Leibman , Linda Lutfiyya , Savitha Madappa , Sivalinganna Manjunath , Robert J. Meister , Bellur Narasimba Prasad , Sasha Preuss , Qungang Qi , Dhanalakshmi Ramachandra , S. Sangeetha , Scott Saracco , Chitresh Sharma , Vijay K. Sharma , Rajani Monnanda Somaiah , Padmini Sudarshana , Rebecca L. Thompson-Mize , Srikanth Venkatachalayya , Tyamagondlu V. Venkatesh , Huai Wang , Jingrui Wu , Xiaoyun Wu , Xiaofeng Sean Yang , Qin Zeng , Jianmin Zhao
发明人： Eric D. Aasen , Mark Scott Abad , Edwards Allen , Thandoni Rao Ambika , Veena S. Anil , Alice Clara Augustine , Stephanie L. Back , Pranesh Badami , Amarjit Basra , Kraig L. Brustad , Molian Deng , Todd Dezwaan , Charles Dietrich , Stephen M. Duff , Karen K. Gabbert , Steve He , Bill L. Hendrix , Hongwu Jia , Vidya Kammaradi Ramanath Bhat , Hongwu Jia , Vidya Kammaradi Ramanath Bhat , Balasulojini Karunanandaa , Kevin R. Kosola , Kempagangaiah Krishnamurthy , Ganesh Kumar , Saritha V. Kuriakose , Shantala Lakkanna , Mark E. Leibman , Linda Lutfiyya , Savitha Madappa , Sivalinganna Manjunath , Robert J. Meister , Bellur Narasimba Prasad , Sasha Preuss , Qungang Qi , Dhanalakshmi Ramachandra , S. Sangeetha , Scott Saracco , Chitresh Sharma , Vijay K. Sharma , Rajani Monnanda Somaiah , Padmini Sudarshana , Rebecca L. Thompson-Mize , Srikanth Venkatachalayya , Tyamagondlu V. Venkatesh , Huai Wang , Jingrui Wu , Xiaoyun Wu , Xiaofeng Sean Yang , Qin Zeng , Jianmin Zhao
IPC分类号： A01H1/02 , C12N15/63 , A01G7/00 , A01H5/00 , A01H5/10 , A23L1/20 , C12N15/29 , C12N5/10
CPC分类号： C12N15/8251 , C12N15/8247 , C12N15/8261 , C12N15/8273 , C12N15/8274 , C12N15/8275 , Y02A40/146
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced trait.
摘要翻译：本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐冷性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

3. 发明授权

US08357833B2 Corn plants and seed enhanced for asparagine and protein 有权
公开(公告)号：US08357833B2
公开(公告)日：2013-01-22
申请号：US13042433
申请日：2011-03-07
申请人： Scott Andersen , James H. Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven E. Screen
发明人： Scott Andersen , James H. Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven E. Screen
IPC分类号： A01H1/00 , C12N15/52 , C07H21/04 , C07K14/415
CPC分类号： C12N15/8251 , C12N9/93
摘要： The present invention relates to a corn plant and seed with enhanced levels of protein and amino acids. The invention also relates to DNA constructs that provide expression in transgenic corn cells of an asparagine synthetase enzyme. The DNA constructs are used in a method to produce transgenic corn plants and seeds and to select for plants and seeds with enhanced levels of protein and amino acids.

4. 发明申请

US20090081353A1 CORN PLANTS AND SEED ENHANCED FOR ASPARAGINE AND PROTEIN 审中-公开
标题翻译：玉米植物和种子增强了阿糖胞苷和蛋白质
公开(公告)号：US20090081353A1
公开(公告)日：2009-03-26
申请号：US12106048
申请日：2008-04-18
申请人： Scott Andersen , James Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven Screen
发明人： Scott Andersen , James Crowley , Stephen M. Duff , Bradon J. Fabbri , Qungang Qi , Bo-Xing Qiu , Steven Screen
IPC分类号： A23L1/10 , C12N15/11 , C12N15/82 , A23K1/16 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8251 , C12N9/93
摘要： The present invention relates to a corn plant and seed with enhanced levels of protein and amino acids. The invention also relates to DNA constructs that provide expression in transgenic corn cells of an asparagine synthetase enzyme. The DNA constructs are used in a method to produce transgenic corn plants and seeds and to select for plants and seeds with enhanced levels of protein and amino acids.
摘要翻译：本发明涉及具有增强蛋白质和氨基酸水平的玉米植物和种子。本发明还涉及在天冬酰胺合成酶的转基因玉米细胞中提供表达的DNA构建体。 DNA构建体用于生产转基因玉米植物和种子的方法，并选择具有增强蛋白质和氨基酸水平的植物和种子。

5. 发明授权

US07238855B2 TyrA genes and uses thereof 有权
标题翻译： TyrA基因及其用途
公开(公告)号：US07238855B2
公开(公告)日：2007-07-03
申请号：US10137310
申请日：2002-05-03
申请人： Henry E. Valentin , Timothy A. Mitsky , Ming Hao , Balasulojini Karunanandaa , Qungang Qi
发明人： Henry E. Valentin , Timothy A. Mitsky , Ming Hao , Balasulojini Karunanandaa , Qungang Qi
IPC分类号： C12N15/82 , A01H5/00
CPC分类号： C12N9/001 , C07K14/47 , C12N9/90 , C12N15/8243
摘要： The present invention is in the field of plant genetics and biochemistry. More specifically, the invention relates to genes associated with the tocopherol biosynthesis pathway. The present invention provides and includes nucleic acid molecules, proteins, and antibodies associated with the genes of the tocopherol biosynthesis pathway. The present invention also provides methods for utilizing such agents, for example in gene isolation, gene analysis and the production of transgenic plants. Moreover, the present invention includes transgenic plants modified to express proteins associated with the tocopherol pathway. In addition, the present invention includes methods for the production of products from the tocopherol biosynthesis pathway.
摘要翻译：本发明在植物遗传学和生物化学领域。更具体地，本发明涉及与生育酚生物合成途径相关的基因。本发明提供并包括与生育酚生物合成途径的基因相关的核酸分子，蛋白质和抗体。本发明还提供了利用这些试剂的方法，例如在基因分离，基因分析和转基因植物的生产中。此外，本发明包括被修饰以表达与生育酚途径相关的蛋白质的转基因植物。此外，本发明包括从生育酚生物合成途径生产产物的方法。

6. 发明授权

US09090882B2 Compositions and methods for enhanced amino acid levels in plants conferred by lysine and/or threonine feedback insensitive aspartate kinase proteins 有权
标题翻译：赖氨酸和/或苏氨酸反馈不敏感天冬氨酸激酶蛋白质赋予植物氨基酸水平的组合物和方法
公开(公告)号：US09090882B2
公开(公告)日：2015-07-28
申请号：US12997860
申请日：2009-06-30
申请人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , William D. Rapp
发明人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , William D. Rapp
IPC分类号： C12N9/12 , C12N15/82
CPC分类号： C12N9/1217 , C12N15/8251 , C12N15/8254
摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feedback-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
摘要翻译：苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。本发明提供了一种通过在开发大豆植物和种子中反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。这些修饰提供了增强植物氮代谢和作物生长性能的方法。

7. 发明申请

US20110191898A1 Compositions and Methods for Enhanced Amino Acid Levels in Plants 有权
标题翻译：植物中增强的氨基酸水平的组成和方法
公开(公告)号：US20110191898A1
公开(公告)日：2011-08-04
申请号：US12997860
申请日：2009-06-30
申请人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , Willam D. Rapp
发明人： James H. Crowley , Barry S. Goldman , Jintai Huang , Qungang Qi , Willam D. Rapp
IPC分类号： C12N15/87 , C07H21/04 , C12N9/12 , C12N5/10 , A01H5/00
CPC分类号： C12N9/1217 , C12N15/8251 , C12N15/8254
摘要： Threonine is an essential amino acid for humans and in the animal feed industry where its levels in feed rations can significantly impact the cost of production of important meat sources, such as swine and poultry. Threonine as well as essential amino acids lysine and methionine are all synthesized via the aspartate family pathway. Aspartate kinase (AK) is the first enzyme in the pathway, and catalyzes the ATP-dependent phosphorylation of aspartate to form β-aspartyl phosphate. AK constitutes the main regulatory step controlling the metabolic flux through the pathway, and is subject to end product inhibition by Lys and/or Thr. The current invention provides a method to produce a transgenic high free threonine soybean via the overexpression of feed-back-resistant AK enzymes in developing soybean plants and seeds. These modifications provide a method to enhance both plant nitrogen metabolism and crop growth performance.
摘要翻译：苏氨酸是人类和动物饲料工业中必不可少的氨基酸，其饲料配给水平可以显着影响重要肉源的生产成本，如猪和家禽。苏氨酸以及必需氨基酸赖氨酸和甲硫氨酸均通过天冬氨酸家族途径合成。天冬氨酸激酶（AK）是途径中的第一个酶，并催化天冬氨酸形成磷酸二酯的ATP依赖性磷酸化。 AK构成控制通过该途径的代谢通量的主要调节步骤，并且受到Lys和/或Thr的最终产物抑制。本发明提供了一种在开发大豆植物和种子中通过反馈抗性AK酶的过表达产生转基因高游离苏氨酸大豆的方法。这些修饰提供了增强植物氮代谢和作物生长性能的方法。

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