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1. 发明申请

US20110265221A1 Transgenic plants with enhanced agronomic traits 审中-公开
标题翻译：具有增强农学性状的转基因植物
公开(公告)号：US20110265221A1
公开(公告)日：2011-10-27
申请号：US12218251
申请日：2008-07-10
申请人： Mark Abad , Jeffrey E. Ahrens , Alice Clara Augustine , Erin Bell , Robert J. Bensen , Paolo Castiglioni , R. Eric Cerny , Shobha Char , Xianfeng Chen , Jaishree M. Chittoor-Vijayanath , Maureen Daley , Jill Deikman , Molian Deng , Todd Dezwaan , Stephen Duff , Michael D. Edgerton , Bradon J. Fabbri , Jason Fenner , Karen K. Gabbert , Meghan Galligan Donnarummo , Barry S. Goldman , Deborah J. Hawkins , Steve He , G. Richard Johnson , Balasulojini Karunanandaa , Garrett J. Lee , Paul Loida , Savitha Madappa , Robert J. Meister , Donald E. Nelson , Anand Pandravada , Obed Patty , Ming Peng , Marie Petracek , M.S. Rajani , Dhanalakshmi Ramachandra , Monica Ravanello , Thomas G. Ruff , Rick A. Sanders , Beth Savidge , Steven H. Schwartz , Padmini Sudarshana , Jindong Sun , Rebecca L. Thompson-Mize , Virginia Ursin , Dale L. Val , Srikanth Venkatachalayya , T.V. Venkatesh , K.R. Vidya , K. Vijayalakshmi , Todd C. Weber , Jingrui Wu , Zhidong Xie , Wei Zheng
发明人： Mark Abad , Jeffrey E. Ahrens , Alice Clara Augustine , Erin Bell , Robert J. Bensen , Paolo Castiglioni , R. Eric Cerny , Shobha Char , Xianfeng Chen , Jaishree M. Chittoor-Vijayanath , Maureen Daley , Jill Deikman , Molian Deng , Todd Dezwaan , Stephen Duff , Michael D. Edgerton , Bradon J. Fabbri , Jason Fenner , Karen K. Gabbert , Meghan Galligan Donnarummo , Barry S. Goldman , Deborah J. Hawkins , Steve He , G. Richard Johnson , Balasulojini Karunanandaa , Garrett J. Lee , Paul Loida , Savitha Madappa , Robert J. Meister , Donald E. Nelson , Anand Pandravada , Obed Patty , Ming Peng , Marie Petracek , M.S. Rajani , Dhanalakshmi Ramachandra , Monica Ravanello , Thomas G. Ruff , Rick A. Sanders , Beth Savidge , Steven H. Schwartz , Padmini Sudarshana , Jindong Sun , Rebecca L. Thompson-Mize , Virginia Ursin , Dale L. Val , Srikanth Venkatachalayya , T.V. Venkatesh , K.R. Vidya , K. Vijayalakshmi , Todd C. Weber , Jingrui Wu , Zhidong Xie , Wei Zheng
IPC分类号： A01H1/02 , C40B30/00 , A01H5/00 , A01H5/10 , C12N5/10 , C12N15/82
CPC分类号： C12N15/8273 , A01H1/02 , C12N15/8241 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12Q1/6895 , C12Q2600/13 , Y02A40/146
摘要： This invention provides recombinant DNA constructs, transgenic plant nuclei and cells with such recombinant DNA construct for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译：本发明提供重组DNA构建体，转基因植物核和具有这样的重组DNA构建体的细胞，用于表达可用于赋予转基因作物植物增强的农艺性状的蛋白质。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

2. 发明申请

US20120227131A1 Transgenic plants with enhanced agronomic traits 有权
标题翻译：具有增强农学性状的转基因植物
公开(公告)号：US20120227131A1
公开(公告)日：2012-09-06
申请号：US13199773
申请日：2011-09-08
申请人： Mark S. Abad , Thomas R. Adams , Ameeta K. Argarwal , James A. Ball , Paolo Castiglioni , Banu Gopalan , Erin Bell , Shobha Char , Robert J. Bensen , Raghava S. Boddupalli , Alessandra Frizzi , Jill Deikman , Robert D'Ordine , Michael D. Edgerton , Xianfeng Chen , Mary Fernandes , Paul S. Chomet , Stephen Duff , Deborah G. Rucker , Changlin Fu , Jeffrey E. Ahrens , Stanton B. Dotson , Richard E. Finger , Jinzhuo Dong , Molian Deng
发明人： Mark S. Abad , Thomas R. Adams , Ameeta K. Argarwal , James A. Ball , Paolo Castiglioni , Banu Gopalan , Erin Bell , Shobha Char , Robert J. Bensen , Raghava S. Boddupalli , Alessandra Frizzi , Jill Deikman , Robert D'Ordine , Michael D. Edgerton , Xianfeng Chen , Mary Fernandes , Paul S. Chomet , Stephen Duff , Deborah G. Rucker , Changlin Fu , Jeffrey E. Ahrens , Stanton B. Dotson , Richard E. Finger , Jinzhuo Dong , Molian Deng
IPC分类号： A01H5/00 , A01H1/02 , C12Q1/68 , C12N5/10 , A01H5/10
CPC分类号： C12N15/8209 , A01H5/10 , C07K14/4702 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8273 , C12N15/8274
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译：本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

3. 发明申请

US20090100536A1 Transgenic plants with enhanced agronomic traits 审中-公开
标题翻译：具有增强农学性状的转基因植物
公开(公告)号：US20090100536A1
公开(公告)日：2009-04-16
申请号：US11879785
申请日：2007-07-18
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
IPC分类号： A01H1/02 , C12N1/00 , C12N5/00 , A01H5/00
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译：本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

4. 发明申请

US20110258734A1 GENE SEQUENCES AND USES THEREOF IN PLANTS 审中-公开
标题翻译：基因序列及其在植物中的应用
公开(公告)号：US20110258734A1
公开(公告)日：2011-10-20
申请号：US13046601
申请日：2011-03-11
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K.R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
IPC分类号： A01H1/02 , A01D45/00 , A01H5/10 , C12N5/10 , A01H5/00
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.
摘要翻译：本发明提供具有用于表达蛋白质的重组DNA的转基因植物细胞，其可用于赋予转基因作物植物增强的农艺性状。本发明还提供包含转基因植物细胞的转基因植物和后代种子，其中植物被选择用于具有增强的性状，所述性状选自由增强的水利用效率，增强的耐寒性，增加的产量，增强的氮利用效率，增强的氮利用效率，种子蛋白和增强种子油。还公开了用于制备具有增强特征的转基因种子和植物的方法。

5. 发明申请

US20130074202A1 GENE SEQUENCES AND USES THEREOF IN PLANTS 审中-公开
公开(公告)号：US20130074202A1
公开(公告)日：2013-03-21
申请号：US13470467
申请日：2012-05-14
申请人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K. R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
发明人： Thomas Adams , Ameeta K. Agarwa , Jeffrey Ahrens , James A. Ball , Amarjit Basra , Erin Bell , Terry L. Bradshaw , Paul S. Chomet , James H. Crowley , Jill Deikman , Molian Deng , Meghan Donnarummo , Kimberly Faye Zobrist Duff , Stephen Duff , Michael D. Edgerton , Banu Gopalan , Xiaoping He , Mackenzie Heal , Brendan S. Hinchey , Shihshieh Huang , Richard G. Johnson , Vincent Jung , Keith Kretzmer , Lucille B. Laccetti , Chao Qiang Lai , Garrett J. Lee , Michael H. Luethy , Timothy J. Leland , Jingdong Liu , Bin Lu , Adrian A. Lund , Linda L. Madson , Kathleen P. Malloy , Marcus Paul McNabnay , Manchikanti Padmavati , Thomas Ruff , William Start , Daniel Tennessen , Carl P. Urwin , Gabriela Vaduva , K. R. Vidya , Steven Thomas Voss , Christine Voyles , Zhango Xin , Nanfei Xu , Qingzhang Xu , Qiang Zhang , Yajuan Zhao , Li Zhou
IPC分类号： C12N15/82 , A01D45/00
CPC分类号： C12N15/8279 , A01D45/00 , A01N25/34 , A01N31/16 , A01N41/10 , A01N43/40 , A01N43/653 , A01N43/78 , A01N43/80 , A01N55/02 , C07K14/415 , C12N9/0071 , C12N15/8241 , C12N15/8243 , C12N15/8247 , C12N15/8251 , C12N15/8261 , C12N15/8271 , C12N15/8273 , C12N15/8274 , C12N15/8275 , C12Y114/12017 , A01N25/10 , A01N25/02 , A01N2300/00
摘要： This invention provides transgenic plant cells with recombinant DNA for expression of proteins that are useful for imparting enhanced agronomic trait(s) to transgenic crop plants. This invention also provides transgenic plants and progeny seed comprising the transgenic plant cells where the plants are selected for having an enhanced trait selected from the group of traits consisting of enhanced water use efficiency, enhanced cold tolerance, increased yield, enhanced nitrogen use efficiency, enhanced seed protein and enhanced seed oil. Also disclosed are methods for manufacturing transgenic seed and plants with enhanced traits.

6. 发明申请

US20050251882A1 Sucrose phosphate synthase nucleic acid molecules and uses therefor 审中-公开
标题翻译：蔗糖磷酸合成酶核酸分子及其用途
公开(公告)号：US20050251882A1
公开(公告)日：2005-11-10
申请号：US10336263
申请日：2003-01-03
申请人： Robert D'Ordine , Stanton Dotson , Pamela Sisson , Stephen Duff
发明人： Robert D'Ordine , Stanton Dotson , Pamela Sisson , Stephen Duff
IPC分类号： A01H1/00 , A01H5/00 , C07H21/04 , C12N5/04 , C12N9/10 , C12N15/82
CPC分类号： C12N15/8245 , C12N9/1066
摘要： The present invention relates generally to plant molecular biology and genetic engineering. In one embodiment, the present invention relates to isolated nucleic acids from cyanobacteria encoding sucrose phosphate synthase (SPS) or SPS-like proteins, in another embodiment, the present invention relates to isolated nucleic acids from maize plants encoding sucrose phosphate synthase (SPS) proteins. Each protein disclosed has utility in improving agronomic, horticultural and/or quality traits of plants, including yield.
摘要翻译：本发明一般涉及植物分子生物学和遗传工程。在一个实施方案中，本发明涉及来自编码蔗糖磷酸合成酶（SPS）或SPS样蛋白质的蓝细菌的分离的核酸，在另一个实施方案中，本发明涉及编码蔗糖磷酸合成酶（SPS）蛋白质的玉米植物的分离的核酸。所公开的每种蛋白质都可用于改善植物的农艺，园艺和/或品质性状，包括产量。

7. 发明授权

US06936749B1 Nonsymbiotic plant hemoglobins to maintain cell energy status 失效
标题翻译：非共生植物血红蛋白维持细胞能量状态
公开(公告)号：US06936749B1
公开(公告)日：2005-08-30
申请号：US09720206
申请日：1999-06-24
申请人： Phillip Guy , Stephen Duff , Nie Xianzhou , Robert Hill , Douglas Durnin , Aleksander Sowa
发明人： Phillip Guy , Stephen Duff , Nie Xianzhou , Robert Hill , Douglas Durnin , Aleksander Sowa
IPC分类号： A01H5/00 , A61K35/12 , C07K14/415 , C07K14/805 , C12N1/20 , C12N15/29 , C12N15/82
CPC分类号： C07K14/415 , A61K35/12 , C07K14/805 , C12N1/20 , C12N15/8261 , Y02A40/146
摘要： Nonsymbiotic hemoglobins are broadly present across evolution; however, the function of these proteins is unknown. Cultured maize cells have been transformed to constitutively express a barley hemoglobin gene in either the sense (HB+) or antisense (HB−) orientation. Hemoglobin protein in the transformed cell lines was correspondingly higher or lower than in wild type cells under normal atmospheric conditions. Limiting oxygen availability, by placing the cells in a nitrogen atmosphere for 12 hours, had little effect on the energy status of cells constitutively expressing hemoglobin, but had a pronounced effect on both wild type and HB− cells, where ATP levels declined by 27% and 61% respectively. Energy charge was relatively unaffected by the treatment in HB+ and wild type cells, but was reduced from 0.91 to 0.73 in HB− cells suggesting that the latter were incapable of maintaining their energy status under the low oxygen regime. Similar results were observed with P. aeruginosa cells transformed with an Hb expression vector. It is suggested that nonsymbiotic hemoglobins act to maintain the energy status of cells in low oxygen environments and that they accomplish this effect by promoting glycolytic flux through NADH oxidation, resulting in increased substrate level phosphorylation. Nonsymbiotic hemoglobins are likely ancestors of an early form of hemoglobin that sequestered oxygen in low oxygen environments, providing a source of oxygen to oxidize NADH to provide ATP for cell growth and development. This in turn suggests that cells containing increased levels of Hb protein will survive longer under low oxygen tension or high energy demand.
摘要翻译：非共生血红蛋白在进化中广泛存在; 然而，这些蛋白质的功能是未知的。培养的玉米细胞已被转化以组合式表达大鼠血红蛋白基因（HB ^{+ />）或反义（HB ）方向。在正常大气条件下，转化细胞系中的血红蛋白蛋白质相对较高或低于野生型细胞。通过将细胞置于氮气气氛中12小时来限制氧气供应对组成型表达血红蛋白的细胞的能量状态几乎没有影响，但对野生型和HB 细胞都具有显着的作用， ATP水平分别下降了27％和61％。能量电荷相对不受HB +和/或野生型细胞治疗的影响，但在HB 细胞中从0.91降低至0.73，表明后者不能维持低氧状态下的能量状态。用Hb表达载体转化的铜绿假单胞菌细胞观察到类似的结果。建议非共生血红蛋白起作用以维持低氧环境中细胞的能量状态，并通过促进糖酵解通过NADH氧化来实现这一作用，从而增加底物水平的磷酸化。非共生血红蛋白可能是早期形式的血红蛋白的祖先，其在低氧环境中螯合氧，提供氧气来氧化NADH以提供ATP用于细胞生长和发育。这反过来表明，含有较高水平的Hb蛋白的细胞将在低氧压力或高能量需求下生存更长时间。}

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