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1. 发明授权

US06610540B1 Low oxygen culturing of central nervous system progenitor cells 失效
标题翻译：低氧培养中枢神经系统祖细胞
公开(公告)号：US06610540B1
公开(公告)日：2003-08-26
申请号：US09425462
申请日：1999-10-22
申请人： Marie Csete , John Doyle , Barbara J. Wold , Ron McKay , Lorenz Studer
发明人： Marie Csete , John Doyle , Barbara J. Wold , Ron McKay , Lorenz Studer
IPC分类号： C12N500
CPC分类号： C12N5/0658 , A61K35/12 , A61K38/1816 , A61K48/00 , C12N5/0606 , C12N5/0619 , C12N5/0623 , C12N2500/02 , C12N2501/115 , C12N2501/119 , C12N2501/14 , C12N2503/02
摘要： The present invention relates to the growth of cells in culture under conditions that promote cell survival, proliferation, and/or cellular differentiation. The present inventors have found that proliferation was promoted and apoptosis reduced when cells were grown in lowered oxygen as compared to environmental oxygen conditions traditionally employed in cell culture techniques. Further, the inventors found that differentiation of precursor cells to specific fates also was enhanced in lowered oxygen where a much greater number and fraction of dopaminergic neurons were obtained when mesencephalic precursors were expanded and differentiated in lowered oxygen conditions. Thus at more physiological oxygen levels the proliferation and differentiation of CNS precursors is enhanced, and lowered oxygen is a useful adjunct for ex vivo generation of specific neuron types. Methods and compositions exploiting these findings are described.
摘要翻译：本发明涉及在促进细胞存活，增殖和/或细胞分化的条件下培养细胞的生长。本发明人已经发现，与细胞培养技术中传统使用的环境氧条件相比，当细胞在低氧气中生长时，促进增殖并减少细胞凋亡。此外，本发明人发现，当降低氧气时，前体细胞分化为特定命运也得到增强，其中当中脑前体在降低的氧条件下扩增和分化时，获得了更多数量和分数的多巴胺能神经元。因此，在更多的生理氧水平下，CNS前体的增殖和分化被增强，并且降低的氧是用于特定神经元类型的体外产生的有用的辅助剂。描述了利用这些发现的方法和组合物。

2. 发明申请

US20120094381A1 METHODS OF NEURAL CONVERSION OF HUMAN EMBRYONIC STEM CELLS 有权
标题翻译：人胚胎干细胞神经转换方法
公开(公告)号：US20120094381A1
公开(公告)日：2012-04-19
申请号：US13201137
申请日：2010-02-17
申请人： Stuart Chambers , Lorenz Studer
发明人： Stuart Chambers , Lorenz Studer
IPC分类号： C12N5/071 , C12N5/0793 , C12N5/079
CPC分类号： C12N5/0619 , C12N2500/90 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2501/415 , C12N2501/60 , C12N2501/998 , C12N2506/02 , C12N2506/45
摘要： The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.
摘要翻译：本发明一般涉及干细胞的细胞生物学领域，更具体地涉及多能或多潜能干细胞的定向分化，包括人胚胎干细胞（hESC），体细胞干细胞和诱导的人多能干细胞（hiPSC），其使用新颖的文化条件。具体地，提供了用于获得神经组织，底板细胞和细胞凋亡的方法，包括在hESC中诱导神经平板发育以获得中脑多巴胺（DA）神经元，运动神经元和感觉神经元。此外，使用本发明的方法获得的神经平板组织预期用于与其他组织的共培养物，作为用于移动分化途径的诱导剂，即图案化。

3. 发明授权

US09487751B2 Method to isolate, identify, and use embryonic stem cells directed to forebrain interneuron fate 有权
标题翻译：用于分离，鉴定和使用针对前脑中间神经元命运的胚胎干细胞的方法
公开(公告)号：US09487751B2
公开(公告)日：2016-11-08
申请号：US13376039
申请日：2010-06-02
申请人： Stewart A. Anderson , Asif Maroof , Lorenz Studer
发明人： Stewart A. Anderson , Asif Maroof , Lorenz Studer
IPC分类号： C12N5/10 , C12N15/113 , C12N15/63 , C12N5/079 , C12N5/0793 , A61K35/30 , A61K35/12
CPC分类号： C12N5/0619 , A61K35/12 , A61K35/30 , C12N2500/44 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2506/02 , C12N2506/45 , C12N2510/00 , C12N2533/90
摘要： The present invention relates to methods of isolating a purified or enriched population of cortical or striatal immature interneuron progenitor cells and the isolated purified or enriched population of immature interneuron progenitor cells. Methods of treating a condition mediated by a loss or deficiency of interneuron function using the purified or enriched population of immature interneuron progenitor cells are also disclosed.
摘要翻译：本发明涉及分离纯化或富集的皮质或纹状体未成熟中间神经祖细胞群体和分离的纯化或富集的未成熟中间神经祖细胞群体的方法。还公开了使用纯化或富集的未成熟中间神经祖细胞群体治疗由中间神经功能丧失或缺乏介导的病症的方法。

4. 发明授权

US09453198B2 Method of nociceptor differentiation of human embryonic stem cells and uses thereof 有权
标题翻译：人胚胎干细胞伤害感受器分化方法及其用途
公开(公告)号：US09453198B2
公开(公告)日：2016-09-27
申请号：US13697274
申请日：2011-05-19
申请人： Lorenz Studer , Stuart M. Chambers , Yuchen Qi , Yvonne Marissa Mica
发明人： Lorenz Studer , Stuart M. Chambers , Yuchen Qi , Yvonne Marissa Mica
IPC分类号： C12N5/00 , C12N5/0793 , C12N5/071
CPC分类号： C12N5/062 , C12N5/0618 , C12N5/0626 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/40 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/45 , G01N33/5023 , G01N33/5058
摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.
摘要翻译：本发明涉及干细胞生物学领域，特别是多能干细胞或多能干细胞的细胞特异性分化，其可包括但不限于人胚胎干细胞（hESC），人诱导多能干细胞（hiPSC ），体细胞干细胞，癌症干细胞或能够谱系特异性分化的任何其他细胞。具体描述的是使用新的培养条件将hESC和/或hiPSC的谱系特异性分化指向伤害感受器（即伤害感受器细胞）的方法。使用本发明的方法制备的伤害感受器进一步考虑用于各种用途，包括但不限于用于体外药物发现测定，疼痛研究以及作为逆转外周神经系统疾病或损害的治疗剂（PNS）。此外，提供用于从人多能干细胞产生用于疾病建模的黑素细胞的组合物和方法。

5. 发明授权

US08642334B2 Methods of neural conversion of human embryonic stem cells 有权
标题翻译：人胚胎干细胞神经转换方法
公开(公告)号：US08642334B2
公开(公告)日：2014-02-04
申请号：US13201137
申请日：2010-02-17
申请人： Stuart Chambers , Lorenz Studer
发明人： Stuart Chambers , Lorenz Studer
IPC分类号： C12N5/00 , C12N5/02
CPC分类号： C12N5/0619 , C12N2500/90 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2501/415 , C12N2501/60 , C12N2501/998 , C12N2506/02 , C12N2506/45
摘要： The present invention relates generally to the field of cell biology of stem cells, more specifically the directed differentiation of pluripotent or multipotent stem cells, including human embryonic stem cells (hESC), somatic stem cells, and induced human pluripotent stem cells (hiPSC) using novel culture conditions. Specifically, methods are provided for obtaining neural tissue, floor plate cells, and placode including induction of neural plate development in hESCs for obtaining midbrain dopamine (DA) neurons, motorneurons, and sensory neurons. Further, neural plate tissue obtained using methods of the present inventions are contemplated for use in co-cultures with other tissues as inducers for shifting differentiation pathways, i.e. patterning.
摘要翻译：本发明一般涉及干细胞的细胞生物学领域，更具体地涉及多能或多潜能干细胞的定向分化，包括人胚胎干细胞（hESC），体细胞干细胞和诱导的人多能干细胞（hiPSC），其使用新颖的文化条件。具体地，提供了用于获得神经组织，底板细胞和细胞凋亡的方法，包括在hESC中诱导神经平板发育以获得中脑多巴胺（DA）神经元，运动神经元和感觉神经元。此外，使用本发明的方法获得的神经平板组织预期用于与其他组织的共培养物作为用于移动分化途径的诱导剂，即图案化。

6. 发明申请

US20120148549A1 METHOD TO ISOLATE, IDENTIFY, AND USE EMBRYONIC STEM CELLS DIRECTED TO FOREBRAIN INTERNEURON FATE 有权
标题翻译：分离，鉴定和使用指导胚胎干细胞的方法
公开(公告)号：US20120148549A1
公开(公告)日：2012-06-14
申请号：US13376039
申请日：2010-06-02
申请人： Stewart A. Anderson , Asif Maroof , Lorenz Studer
发明人： Stewart A. Anderson , Asif Maroof , Lorenz Studer
IPC分类号： A61K35/12 , C12N5/10 , A61P25/18 , A61P25/08 , A61P25/00 , A61P25/22 , C12P21/06 , A61P25/16
CPC分类号： C12N5/0619 , A61K35/12 , A61K35/30 , C12N2500/44 , C12N2501/115 , C12N2501/15 , C12N2501/155 , C12N2501/41 , C12N2506/02 , C12N2506/45 , C12N2510/00 , C12N2533/90
摘要： The present invention relates to methods of isolating a purified or enriched population of cortical or striatal immature interneuron progenitor cells and the isolated purified or enriched population of immature interneuron progenitor cells. Methods of treating a condition mediated by a loss or deficiency of interneuron function using the purified or enriched population of immature interneuron progenitor cells are also disclosed.
摘要翻译：本发明涉及分离纯化或富集的皮质或纹状体未成熟中间神经祖细胞群体和分离的纯化或富集的未成熟中间神经祖细胞群体的方法。还公开了使用纯化或富集的未成熟中间神经祖细胞群体治疗由中间神经功能丧失或缺乏介导的病症的方法。

7. 发明申请

US20130183674A1 METHOD OF NOCICEPTOR DIFFERENTIATION OF HUMAN EMBRYONIC STEM CELLS AND USES THEREOF 有权
标题翻译：人胚胎干细胞标记分化方法及其用途
公开(公告)号：US20130183674A1
公开(公告)日：2013-07-18
申请号：US13697274
申请日：2011-05-19
申请人： Lorenz Studer , Stuart M. Chambers
发明人： Lorenz Studer , Stuart M. Chambers
IPC分类号： C12N5/0793
CPC分类号： C12N5/062 , C12N5/0618 , C12N5/0626 , C12N2501/13 , C12N2501/15 , C12N2501/155 , C12N2501/16 , C12N2501/40 , C12N2501/415 , C12N2501/727 , C12N2506/02 , C12N2506/45 , G01N33/5023 , G01N33/5058
摘要： The present invention relates to the field of stem cell biology, in particular the linage specific differentiation of pluripotent or multipotent stem cells, which can include, but is not limited to, human embryonic stem cells (hESC), human induced pluripotent stem cells (hiPSC), somatic stem cells, cancer stem cells, or any other cell capable of lineage specific differentiation. Specifically described are methods to direct the lineage specific differentiation of hESC and/or hiPSC to nociceptors (i.e. nociceptor cells) using novel culture conditions. The nociceptors made using the methods of the present invention are further contemplated for various uses including, but limited to, use in in vitro drug discovery assays, pain research, and as a therapeutic to reverse disease of, or damage to, the peripheral nervous system (PNS). Further, compositions and methods are provided for producing melanocytes from human pluripotent stem cells for use in disease modeling.
摘要翻译：本发明涉及干细胞生物学领域，特别是多能干细胞或多能干细胞的细胞特异性分化，其可包括但不限于人胚胎干细胞（hESC），人诱导多能干细胞（hiPSC ），体细胞干细胞，癌症干细胞或能够谱系特异性分化的任何其他细胞。具体描述的是使用新的培养条件将hESC和/或hiPSC的谱系特异性分化指向伤害感受器（即伤害感受器细胞）的方法。使用本发明的方法制备的伤害感受器进一步考虑用于各种用途，包括但不限于用于体外药物发现测定，疼痛研究以及作为逆转外周神经系统疾病或损害的治疗剂（PNS）。此外，提供用于从人多能干细胞产生用于疾病建模的黑素细胞的组合物和方法。

8. 发明授权

US06787356B1 Cell expansion system for use in neural transplantation 失效
标题翻译：用于神经移植的细胞扩增系统
公开(公告)号：US06787356B1
公开(公告)日：2004-09-07
申请号：US09744384
申请日：2001-03-16
申请人： Lorenz Studer , Ron D. McKay
发明人： Lorenz Studer , Ron D. McKay
IPC分类号： C12N502
CPC分类号： C12N5/0619 , C12N2500/10 , C12N2500/38 , C12N2501/815
摘要： The invention provides a method of culturing cells which includes a proliferating step in which the number of precursor cells is expanded and a differentiating step in which the expanded precursor cells develop into neuronal cells. The proliferating step includes the step of incubating the precursor cells in proliferating medium which includes basic fibroblast growth factor (bFGF). The differentiating step includes incubating the precursor cells in differentiation media in a manner effective to form a cellular aggregate that is not adhered to any surface of the incubation vessel. In a preferred embodiment, the cells arc incubated in a roller tube. The differentiation media can also include at least one differentiating agent. The invention also provides a method for treating a neurological disorder, such as Parkinson's disease, a method of introducing a gene product into a brain of a patient, an assay for neurologically active substances, and a cell culture.
摘要翻译：本发明提供一种培养细胞的方法，其包括扩增前体细胞数量的增殖步骤和扩增的前体细胞发育成神经元细胞的分化步骤。增殖步骤包括将前体细胞在包括碱性成纤维细胞生长因子（bFGF）的增殖培养基中孵育的步骤。微分步骤包括以有效形成未附着于培养容器的任何表面的细胞聚集体的方式将分化培养基中的前体细胞孵育。在优选的实施方案中，将细胞在辊管中保温。分化培养基还可以包括至少一种分化剂。本发明还提供了治疗诸如帕金森病的神经障碍的方法，将基因产物引入患者脑中的方法，神经活性物质的测定和细胞培养。

9. 发明申请

US20150086481A1 DIAGNOSIS AND TREATMENT OF PARKINSON'S DISEASE 审中-公开
标题翻译：诊断和治疗帕金森病
公开(公告)号：US20150086481A1
公开(公告)日：2015-03-26
申请号：US14235534
申请日：2012-07-27
申请人： Yosif Ganat , Lorenz Studer
发明人： Yosif Ganat , Lorenz Studer
IPC分类号： A61K47/48 , A61K31/197 , A61K51/08
CPC分类号： A61K51/088 , A61K31/197 , A61K38/17 , A61K47/64 , G01N33/6896 , G01N2333/988 , G01N2800/2835
摘要： GUCY2C receptor ligands conjugated to diagnostic and/or therapeutic moieties, methods of making such GUCY2C receptor ligands conjugates, and methods of using such GUCY2C receptor ligands conjugates, such as in the diagnosis and/or treatment of Parkinson's disease, are described.
摘要翻译：描述了与诊断和/或治疗部分缀合的GUCY2C受体配体，制备这种GUCY2C受体配体共轭物的方法，以及使用这种GUCY2C受体配体缀合物的方法，例如在帕金森病的诊断和/或治疗中。

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