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1. 发明申请

US20110014662A1 Methods for producing hyaluronan in a recombinant host cell 有权
标题翻译：在重组宿主细胞中产生透明质酸的方法
公开(公告)号：US20110014662A1
公开(公告)日：2011-01-20
申请号：US12891548
申请日：2010-09-27
申请人： Alan Sloma , Leslie Naggiar , Regine Behr , William Widner , Maria Tang , David Sternberg , Linda Sternberg , Stephen Brown
发明人： Alan Sloma , Leslie Naggiar , Regine Behr , William Widner , Maria Tang , David Sternberg , Linda Sternberg , Stephen Brown
IPC分类号： C12P19/04
CPC分类号： C12P19/26 , C12N9/1051 , C12N15/52
摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase
摘要翻译：本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选的一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列

2. 发明申请

US20110189737A1 Methods for producing hyaluronan in a recombinant host cell 有权
公开(公告)号：US20110189737A1
公开(公告)日：2011-08-04
申请号：US13084230
申请日：2011-04-11
申请人： Alan Sloma , Regine Behr , William Widner , Maria Tang , David Sternberg , Stephen Brown , Leslie Naggiar , Linda Sternberg
发明人： Alan Sloma , Regine Behr , William Widner , Maria Tang , David Sternberg , Stephen Brown , Leslie Naggiar , Linda Sternberg
IPC分类号： C12P19/04
CPC分类号： C12P19/26 , C12N9/1051 , C12N15/52
摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase

3. 发明申请

US20120149067A1 Methods for producing hyaluronan in a recombinant host cell 有权
标题翻译：在重组宿主细胞中产生透明质酸的方法
公开(公告)号：US20120149067A1
公开(公告)日：2012-06-14
申请号：US13401663
申请日：2012-02-21
申请人： Alan Sloma , Leslie Naggiar , Regine Behr , William Widner , Maria Tang , David Sternberg , Linda Sternberg , Stephen Brown
发明人： Alan Sloma , Leslie Naggiar , Regine Behr , William Widner , Maria Tang , David Sternberg , Linda Sternberg , Stephen Brown
IPC分类号： C12P19/28
CPC分类号： C12P19/26 , C12N9/1051 , C12N15/52
摘要： The present invention relates to methods for producing a hyaluronic acid, comprising: (a) cultivating a Bacillus host cell under conditions suitable for production of the hyaluronic acid, wherein the Bacillus host cell comprises a nucleic acid construct comprising a hyaluronan synthase encoding sequence operably linked to a promoter sequence foreign to the hyaluronan synthase encoding sequence; and (b) recovering the hyaluronic acid from the cultivation medium. The present invention also relates to an isolated nucleic acid sequence encoding a hyaluronan synthase operon comprising a hyaluronan synthase gene and a UDP-glucose 6-dehydrogenase gene, and optionally one or more genes selected from the group consisting of a UDP-glucose pyrophosphorylase gene, UDP-N-acetylglucosamine pyrophosphorylase gene, and glucose-6-phosphate isomerase gene. The present invention also relates to isolated nucleic acid sequences encoding a UDP-glucose 6-dehydrogenase, UDP-glucose pyrophosphorylase, and UDP-N-acetylglucosamine pyrophosphorylase
摘要翻译：本发明涉及透明质酸的制备方法，其包括：（a）在适于产生透明质酸的条件下培养芽孢杆菌属宿主细胞，其中所述芽孢杆菌宿主细胞包含可操作地连接的透明质酸合酶编码序列的核酸构建体涉及透明质酸合酶编码序列外源的启动子序列; 和（b）从培养基中回收透明质酸。本发明还涉及编码包含透明质酸合酶基因和UDP-葡萄糖-6-脱氢酶基因的透明质酸合酶操纵子的分离的核酸序列，以及任选地一种或多种选自UDP-葡萄糖焦磷酸化酶基因， UDP-N-乙酰葡糖胺焦磷酸化酶基因和葡萄糖-6-磷酸异构酶基因。本发明还涉及编码UDP-葡萄糖-6-脱氢酶，UDP-葡萄糖焦磷酸化酶和UDP-N-乙酰葡糖胺焦磷酸化酶的分离的核酸序列

4. 发明申请

US20100330618A1 Methods for producing biological substances in pigment-deficient mutants of bacillus cells 有权
公开(公告)号：US20100330618A1
公开(公告)日：2010-12-30
申请号：US12703550
申请日：2010-02-10
申请人： Maria Tang , Alan Sloma , Leslie Naggiar , David Sternberg , Linda Sternberg , Regine Behr
发明人： Maria Tang , Alan Sloma , Leslie Naggiar , David Sternberg , Linda Sternberg , Regine Behr
IPC分类号： C12P21/00 , C12N1/21 , C12N15/74
CPC分类号： C12N9/0077 , C07K14/32 , C12P1/04 , C12P19/00 , C12P21/02
摘要： The present invention relates to methods of producing a heterologous biological substance, comprising: (a) cultivating a mutant of a parent Bacillus cell under conditions conducive for the production of the heterologous biological substance, wherein (i) the mutant cell comprises a first nucleic acid sequence directing synthesis of the heterologous biological substance and a second nucleic acid sequence comprising a modification of at least one of the genes cypX and yvmC, which are involved in the production of a red pigment, and (ii) the mutant cell is deficient in the production of the red pigment compared to the parent Bacillus cell when cultivated under the same conditions; and (b) recovering the heterologous biological substance from the cultivation medium. The present invention also relates to mutants of Bacillus cells and methods for producing the mutants.

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